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Characterization of low-N responses in maize(Zea mays L.) cultivars with contrasting nitrogen use efficiency in the North China Plain

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Journal of Integrative Agriculture 2019年第9期18卷 2141-2152页

作者： LI xiang-ling GUO Li-guo ZHOU Bao-yuan tang xiang-ming CHEN Cong-cong ZHANG Lei ZHANG Shao-yun LI Chong-feng XIAO Kai DONG Wei-xin YIN Bao-zhong ZHANG Yue-chen College of Agronomy Hebei Agricultural University/Key Laboratory of Crop Growth Regulation of Hebei ProvinceBaoding 071001P.R.China Institute of Crop Sciences Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and ProductionMinistry of Agriculture and Rural AffairsBeijing 100081P.R.China College of Plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China Teaching Support and Service Center Hebei Radio and TV UniversityShijiazhuang 050080P.R.China

Over-use of N fertilizer in crop production has resulted in a series of environmental problems in the North China Plain(NCP).Thus,improvement of nitrogen use efficiency(NUE)in summer maize has become an effective strategy for promoting sustainable agriculture in this region.Using twenty maize cultivars,plant dry matter production,N absorption and accumulation,yield formation,and NUE in summer maize were investigated under three N levels in two growing seasons.Based on their yield and yield components,these maize cultivars were categorized into four groups including efficient-efficient(EE)cultivars,high-nitrogen efficient(HNE)cultivars,low-nitrogen efficient(LNE)cultivars and nonefficient-nonefficient(NN)cultivars.In both two seasons,the EE cultivars improved grain yield together with increased plant biomass,and enhanced accumulative amounts as well as higher average grain yields than the other cultivar groups under deficient-N conditions.Significant correlations were observed between yield and kernel numbers(KN),dry matter(DM)amount and N accumulation at both post-silking and maturity stages.DM and N accumulation at late growth stage(i.e.,from silking to maturity)contributed largely to the enhanced yield capacity and improved NUE under N-deficient conditions.Compared with the NN cultivars,the EE cultivars also showed increased N assimilation amount(NAA)and N remobilization content(NRC),and elevated N remobilization efficiency(NRE),NUE and nitrogen partial factor productivity(PFPN).Our investigation has revealed N-associated physiological processes and may provide guidance for cultivation and breeding of high yield and NUE summer maize under limited N conditions in the NCP.

关键词： summer maize N deficiency yield dry matter production N accumulative amount

太湖北部湖滨带芦苇湿地夏季水质时空变化特征分析

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环境生态学 2024年第3期6卷 99-106页

作者：吕家铭杨桂军邵克强高光汤祥明中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室江苏南京210008 中国科学院大学资源与环境学院北京100190 江南大学环境与土木工程学院江苏无锡214122

为了解太湖北部湖滨带芦苇湿地夏季水质时空变化,于2022年6—9月,在芦苇湿地时空分布调查的基础上,分别采集典型湖滨带芦苇湿地表层水体样品,测定pH、总氮(TN)、总磷(TP)、氨氮(NH+4-N)、高锰酸盐指数(COD Mn)、叶绿素a(Chl-a)等水质理... 详细信息

为了解太湖北部湖滨带芦苇湿地夏季水质时空变化,于2022年6—9月,在芦苇湿地时空分布调查的基础上,分别采集典型湖滨带芦苇湿地表层水体样品,测定pH、总氮(TN)、总磷(TP)、氨氮(NH+4-N)、高锰酸盐指数(COD Mn)、叶绿素a(Chl-a)等水质理化指标,并对湿地水质时空变化特征和水体富营养化状态进行分析。结果表明,所调查的7片芦苇湿地中pH、溶解氧(DO)、电导率(EC)、水温(WT)月间变化差异均极显著(p0.05)。所有水质理化指标在竺山湾、月亮湾、梅梁湾和贡湖湾芦苇湿地间均具有显著差异性(p<0.05)。湿地夏季水体基本呈中度或重度富营养化状态,时间上8月水质最差,地理位置上月亮湾最差。太湖北部湖滨带芦苇湿地水体属于磷限制型,限制磷元素的输入可有效控制湖滨带苇区水体富营养化的程度。

关键词：太湖芦苇湿地水质评价综合营养状态指标法时空变化

Corrosion behaviors and kinetics of nanoscale zero-valent iron in water:A review

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Journal of Environmental Sciences 2024年第1期135卷 391-406页

作者： Chenliu tang Xingyu Wang Yufei Zhang Nuo Liu xiang Hu Research Group of Water Pollution Control and Water Reclamation College of Chemical EngineeringBeijing University of Chemical TechnologyBeijing 100029China Shanghai Collaborative Innovation Centre for WEEE Recycling School of Resources and Environmental EngineeringShanghai Polytechnic UniversityShanghai 201209China

Knowledge on corrosion behaviors and kinetics of nanoscale zero-valent iron(nZVI)in aquatic environment is particularly significant for understanding the reactivity,longevity and stability of nZVI,as well as providing theoretical guidance for developing a cost-effective nZVI-based technology and designing large-scale applications.Herein,this review gives a holistic overview on the corrosion behaviors and kinetics of nZVI in water.Firstly,Eh-pH diagram is introduced to predict the thermodynamics trend of iron corrosion.The morphological,structural,and compositional evolution of(modified-)nZVI under different environmental conditions,assisted with microscopic and spectroscopic evidence,is then summarized.Afterwards,common analytical methods and characterization technologies are categorized to establish time-resolved corrosion kinetics of nZVI in water.Specifically,stable models for calculating the corrosion rate constant of nZVI as well as electrochemical methods for monitoring the redox reaction are discussed,emphasizing their capabilities in studying the dynamic iron corrosion processes.Finally,in the future,more efforts are encouraged to study the corrosion behaviors of nZVI in long-term practical application and further build nanoparticles with precisely tailored properties.We expect that our work can deepen the understanding of the nZVI chemistry in aquatic environment.

关键词： Nanoscale zero-valent iron(nZVI) Corrosion behaviors Corrosion kinetics nZVI evolution

Prognostic value of neutrophil-to-lymphocyte ratio in end-stage liver disease:A meta-analysis

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World Journal of Hepatology 2024年第3期16卷 477-489页

作者： xiang-Hao Cai Yun-ming tang Shu-Ru Chen Jia-Hui Pang Yu-Tian Chong Hong Cao Xin-Hua Li Department of Infectious Disease Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina

BACKGROUND The neutrophil-to-lymphocyte ratio(NLR)is commonly utilized as a prognostic indicator in end-stage liver disease(ESLD),encompassing conditions like liver failure and decompensated cirrhosis.Nevertheless,some studies have contested the prognostic value of NLR in ESLD.AIM To investigate the ability of NLR to predict ESLD.METHODS Databases,such as Embase,PubMed,Web of Science,Cochrane Library,China National Knowledge Infrastructure,Weipu,and Wanfang,were comprehensively searched to identify studies published before October 2022 assessing the prognostic ability of NLR to predict mortality in patients with ESLD.Effect sizes were calculated using comprehensive meta-analysis software and SATAT 15.1.RESULTS A total of thirty studies involving patients with end-stage liver disease(ESLD)were included in the evaluation.Among the pooled results of eight studies,it was observed that the Neutrophil-to-Lymphocyte Ratio(NLR)was significantly higher in non-survivors compared to survivors(random-effects model:standardized mean difference=1.02,95%confidence interval=0.67-1.37).Additionally,twenty-seven studies examined the associations between NLR and mortality in ESLD patients,reporting either hazard ratios(HR)or odds ratios(OR).The combined findings indicated a link between NLR and ESLD mortality(randomeffects model;univariate HR=1.07,95%CI=1.05-1.09;multivariate HR=1.07,95%CI=1.07-1.09;univariate OR=1.29,95%CI=1.18-1.39;multivariate OR=1.29,95%CI=1.09-1.49).Furthermore,subgroup and meta-regression analyses revealed regional variations in the impact of NLR on ESLD mortality,with Asian studies demonstrating a more pronounced effect.CONCLUSION Increased NLR in patients with ESLD is associated with a higher risk of mortality,particularly in Asian patients.NLR is a useful prognostic biomarker in patients with ESLD.

关键词： Neutrophil-to-lymphocyte ratio End stage liver diseases Prognosis Meta-analysis Mortality

Endoscopic-ultrasound-guided biliary drainage with placement of electrocautery-enhanced lumen-apposing metal stent for palliation of malignant biliary obstruction:Updated meta-analysis

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World Journal of Gastrointestinal Surgery 2024年第3期16卷 907-920页

作者： Zu-xiang Peng Fang-Fang Chen Wen tang Xu Zeng Hong-Juan Du Ru-Xian Pi Hong-ming Liu Xiao-Xiao Lu Department of Hepatobiliary Surgery Daping HospitalArmy Medical UniversityChongqing 400042China Department of Oncology Chongqing General HospitalChongqing UniversityChongqing 401147China Department of Gastroenterology and Hepatobiliary Surgery People’s Hospital of Fenggang CountyGuizhou 564200Guizhou ProvinceChina Hepatobiliary and Pancreatic Tumor Center Chongqing University Cancer HospitalChongqing 400030China

BACKGROUND Endoscopic ultrasound-guided biliary drainage using electrocautery-enhanced(ECE)delivery of lumen-apposing metal stent(LAMS)is gradually being re-cognized as a viable palliative technique for malignant biliary obstruction after endoscopic retrograde cholangiopancreatography(ERCP)failure.However,most of the studies that have assessed its efficacy and safety were small and hetero-geneous.Prior meta-analyses of six or fewer studies that were published 2 years ago were therefore underpowered to yield convincing evidence.AIM To update the efficacy and safety of ECE-LAMS for treatment of biliary ob-struction after ERCP failure.METHODS We searched PubMed,EMBASE,and Scopus databases from the inception of the ECE technique to May 13,2022.Primary outcome measure was pooled technical success rate,and secondary outcomes were pooled rates of clinical success,re-intervention,and adverse events.Meta-analysis was performed using a random-effects model following Freeman-Tukey double-arcsine transformation in R soft-ware(version 4.1.3).RESULTS Fourteen eligible studies involving 620 participants were ultimately included.The pooled rate of technical success was 96.7%,and clinical success was 91.0%.Adverse events were reported in 17.5%of patients.Overall reinter-vention rate was 7.3%.Subgroup analyses showed results were generally consistent.CONCLUSION ECE-LAMS has favorable success with acceptable adverse events in relieving biliary obstruction when ERCP is impossible.The consistency of results across most subgroups suggested that this is a generalizable approach.

关键词： Biliary obstruction Biliary drainage Electrocautery-enhanced lumen-apposing metal stents Endoscopic ultrasound Endoscopic retrograde cholangiopancreatography failure

Facet-dependent transformation and toxicity of nanoscale zinc oxide in the synthetic saliva

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Journal of Environmental Sciences 2024年第5期139卷 170-181页

作者： xiang Zhang Yunpeng Huang Jikun Wang Jie tang YangMei Nali Zhu Zhigang Li Lingxiangyu Li YaweiWang School of Environment Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Key Laboratory of Systems Health Science of Zhejiang Province Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Department of Chemistry University of PennsylvaniaPhiladelphia 19104USA

The nanoscale zinc oxide(n-ZnO)was used in food packages due to its superior antibac terial activity,resulting in potential intake of n-ZnO through the digestive system,wherein n-ZnO interacted with saliva.In recent,facet engineering,a technique for controlling the exposed facets,was applied to n-ZnO,whereas risk of n-ZnO with specific exposed facets in saliva was ignored.ZnO nanoflakes(ZnO-0001)and nanoneedles(ZnO-1010)with the pri mary exposed facets of{0001}and{1010}respectively were prepared in this study,investigat ing stability and toxicity of ZnO-0001 and ZnO-1010 in synthetic saliva.Both ZnO-0001 and ZnO-1010 partially transformed into amorphous Zn_(3)(PO_(4))_(2)within 1 hr in the saliva even containing orgnaic components,forming a ZnO-Zn_(3)(PO_(4))_(2)core-shell structure.Neverthe less,ZnO-1010 relative to ZnO-0001 would likely transform into Zn_(3)(PO_(4))_(2),being attributed to superior dissolution of{1010}facet due to its lower vacancy formation energy(1.15 eV than{0001}facet(3.90 eV)).The toxicity of n-ZnO to Caco-2 cells was also dependent on the primary exposed facet;ZnO-0001 caused cell toxicity through oxidative stress,whereas ZnO-1010 resulted in lower cells viability than ZnO-0001 through oxidative stress and mem brane damage.Density functional theory calculations illustrated that·O_(2)^(-)was formed and released on{1010}facet,yet O_(2)^(2-)instead of·O_(2)^(-)was generated on{0001}facet,leading to low oxidative stress from ZnO-0001.All findings demonstrated that stability and toxicity of n-ZnO were dependent on the primary exposed facet,improving our understanding o health risk of nanomaterials.

关键词： The exposed facet Nanoscale ZnO Chemical transformation Synthetic salica Nanotoxicity

Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets

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Animal Nutrition 2024年第1期16卷 23-33页

作者： Wenjie tang Xuan xiang Houfu Wang Wentao Zhou Liuqin He Yulong Yin Tiejun Li College of Animal Science and Technology Hunan Agricultural University Sichuan Academy of Animal Sciences Animal Breeding and Genetics Key Laboratory of Sichuan Province Livestock and Poultry Biological Products Key Laboratory of Sichuan Province Sichuan Animtech Feed Co. Ltd Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical Agriculture Chinese Academy of Sciences College of Advanced Agricultural Science University of Chinese Academy of Sciences Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation Laboratory of Animal Nutrition and Human Health College of Life Sciences Hunan Normal University

This study aimed to determine the regulatory mechanism of dietary zinc lactate (ZL) supplementation on intestinal oxidative stress damage in a paraquat (PQ)-induced piglet model. Twenty-eight piglets (mean body weight 9.51±0.23 kg) weaned at 28 d of age were randomly divided into control, ZL, PQ, and ZL+PQ groups (n=7 in each group). The ZL-supplemented diet had little effect on growth performance under normal physiological conditions. However, under PQ challenge, ZL supplementation significantly improved average daily gain (P<0.05) and reduced the frequency of diarrhea. ZL improved intestinal morphology and ultrastructure by significantly increasing the expression level of the jejunal tight junction protein, zonula occludens-1(ZO-1) (P<0.05), and intestinal zinc transport and absorption in PQ-induced piglets, which reduced intestinal permeability. ZL supplementation also enhanced the expression of antioxidant and antiinflammatory factor-related genes and decreased inflammatory cytokine expression and secretion in PQinduced piglets. Furthermore, ZL treatment significantly inhibited the activation of constitutive androstane receptor (CAR) signaling (P<0.01) in PQ-induced piglets and altered the structure of the gut microbiota, especially by significantly increasing the abundance of beneficial gut microbes, including UCG＿002, Ruminococcus, Rikenellaceae＿RC9＿gut＿group, Christensenellaceae＿R＿7＿group, Treponema, unclassified＿Christensenellaceae, and unclassified＿Erysipelotrichaceae (P <0.05). These data reveal that pre-administration of ZL to piglets can suppress intestinal oxidative stress by improving antioxidant and anti-inflammatory capacity and regulating the crosstalk between CAR signaling and gut microbiota.

关键词： Zinc lactate Intestinal barrier Oxidative stress Inflammation Constitutive androstane receptor Gut microbiota

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基于贝叶斯网络的太湖叶绿素a影响因素分析

环境科学 2023年第5期44卷 2592-2600页

作者：刘杰何云川邓建明汤祥明中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室南京210008 中国科学院大学北京100049

全球变暖加剧了湖泊富营养化问题.太湖作为中国典型的大型富营养化浅水湖泊,有害蓝藻水华问题尤为突出.以太湖作为研究对象,利用1992~2020年气象(气温、风速、降雨量、日照时长)、水质[总氮、总磷(TP)、电导率、pH、化学需氧量]和生物[... 详细信息

全球变暖加剧了湖泊富营养化问题.太湖作为中国典型的大型富营养化浅水湖泊,有害蓝藻水华问题尤为突出.以太湖作为研究对象,利用1992~2020年气象(气温、风速、降雨量、日照时长)、水质[总氮、总磷(TP)、电导率、pH、化学需氧量]和生物[叶绿素a(Chla)]监测数据,基于连续型贝叶斯网络模型构建了Chla的模拟模型,研究太湖不同气象和水质条件下的Chla水平.结果表明,春季“温风比”平均水平为6.67℃·s·m^(-1),ρ(TP)低于0.130 mg·L^(-1)左右时,Chla偏高(>75分位数,下同)的概率小于75%;夏季“温风比”平均水平为10.52℃·s·m^(-1),ρ(TP)低于0.257 mg·L^(-1)左右时,Chla偏高的概率小于75%;秋季ρ(TP)平均水平为0.154 mg·L^(-1),“温风比”小于6.30℃·s·m^(-1)左右时,Chla偏高的概率小于75%.基于以上研究,进一步利用连续型贝叶斯网络模型构建的Chla模型模拟了不同气候变化背景下的营养盐控制目标.为控制太湖Chla处于有害蓝藻水华暴发“低风险”水平,在1992~2000、2001~2010和2011~2020年各年代气候水平背景下TP需要控制的目标浓度阈值分别为春季ρ(TP):0.135、0.115和0.059 mg·L^(-1);夏季ρ(TP):0.174、0.164和0.145 mg·L^(-1)和秋季ρ(TP):0.171、0.162和0.145 mg·L^(-1).结果表明随着全球气候变暖,控制富营养化湖泊有害蓝藻水华需要更加严格的营养盐控制策略,可为太湖开展有害蓝藻水华防控和富营养化治理评估营养盐控制目标提供参考.

关键词：太湖温风比叶绿素a 总磷连续型贝叶斯网络模型富营养化

Deciphering the Shared and Specific Drug Resistance Mechanisms of Anaplastic Lymphoma Kinase via Binding Free Energy Computation

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Research 2024年第1期2023卷 663-674页

作者： Yang Yu Zhe Wang Lingling Wang Qinghua Wang Rongfan tang Sutong xiang Qirui Deng Tingjun Hou Huiyong Sun Department of Medicinal Chemistry China Pharmaceutical UniversityNanjing 210009JiangsuP.R.China Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058ZhejiangP.R.China

Anaplastic lymphoma kinase(ALK),a tyrosine receptor kinase,has been proven to be associated with the occurrence of numerous malignancies.Although there have been already at least 3 generations of ALK inhibitors approved by FDA or in clinical trials,the occurrence of various mutations seriously attenuates the effectiveness of the drugs.Unfortunately,most of the drug resistance mechanisms still remain obscure.Therefore,it is necessary to reveal the bottom reasons of the drug resistance mechanisms caused by the mutations.In this work,on the basis of verifying the accuracy of 2 main kinds of binding free energy calculation methodologies[end-point method of Molecular Mechanics with Poisson-Boltzmann/Generalized Born and Surface Area(MM/PB(GB)SA)and alchemical method of Thermodynamic Integration(TI)],we performed a systematic analysis on the ALK systems to explore the underlying shared and specific drug resistance mechanisms,covering the one-drug-multiple-mutation and multiple-drug-onemutation cases.

关键词： drugs Anaplastic clinical