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Repetitive sequence analysis and karyotyping reveal different genome evolution and speciation of diploid and tetraploid Tripsacum dactyloides

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The Crop Journal 2016年第4期4卷 247-255页

作者： qilin Zhu Zexi Cai qilin tang Weiwei Jin National Maize Improvement Center of China Beijing Key Laboratory of Crop Genetic Improvement Coordinated Research Center for Crop Biology China Agricultural University Maize Research Institute Sichuan Agricultural University

In the subtribe Maydeae, Tripsacum and Zea are closely related genera. Tripsacum is a horticultural crop widely used as pasture forage. Previous studies suggested that Tripsacum might play an important role in maize origin and evolution. However, our understanding of the genomics and the evolution of Tripsacum remains limited. In this study, two diploids,T. dactyloides var. meridionale(2n = 36, MR) and T. dactyloides(2n = 36, DD), and one tetraploid,T. dactyloides(2n = 72, DL) were sequenced by low-coverage genome sequencing followed by graph-based cluster analysis. The results showed that 63.23%, 59.20%, and 61.57% of the respective genome of MR, DD, and DL were repetitive DNA sequence. The proportions of different repetitive sequences varied greatly among the three species. Fluorescence in situ hybridization(FISH) analysis of mitotic metaphase chromosomes with satellite repeats as the probes showed that the FISH signal patterns of DL were more similar to that of DD than to that of MR. Comparative analysis of the repeats also showed that DL shared more common repeat families with DD than with MR. Phylogenetic analysis of internal transcribed spacer region sequences further supported the evolutionary relationship among the three species. Repetitive sequences comparison showed that Tripsacum shared more repeat families with Zea than with Coix and Sorghum. Our study sheds new light on the genomics of Tripsacum and differential speciation in the Poaceae family.

关键词： Tripsacum Low-coverage sequencing Repetitive sequence Karyotype Phylogenetic analysis

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烟碱化合物的固相合成研究

云南大学学报（自然科学版） 2024年第3期46卷 521-526页

作者：冒德寿汤琪琳杨晶尚娜李振杰刘志华汤峨云南省烟草化学重点实验室云南中烟工业有限责任公司技术中心云南昆明650106 教育部自然资源药物化学重点实验室云南大学药学院云南昆明650500

开发了一种高效的烟碱化合物的固相合成新方法.利用交联聚苯乙烯负载磺酸树脂,经树脂氯化,制备出聚苯乙烯负载磺酰氯树脂作为固载有机硫试剂.从3-溴吡啶出发,经锂化酰化、羰基还原、羟基磺酰化、磺酸酯胺化环化4步反应,合成了5个烟碱类... 详细信息

开发了一种高效的烟碱化合物的固相合成新方法.利用交联聚苯乙烯负载磺酸树脂,经树脂氯化,制备出聚苯乙烯负载磺酰氯树脂作为固载有机硫试剂.从3-溴吡啶出发,经锂化酰化、羰基还原、羟基磺酰化、磺酸酯胺化环化4步反应,合成了5个烟碱类化合物,总产率为52%~66%,产物结构经1H NMR鉴定确认.方法具有产率高、操作简单,树脂可以回收再用,具有工业自动化生产的应用前景等特点.

关键词：烟碱固相合成交联聚苯乙烯有机硫试剂

Amplitude-Integrated Electroencephalography Predicts Outcome in Patients with Coma After Acute Brain Injury

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Neuroscience Bulletin 2018年第4期34卷 639-646页

作者： Wendong You qilin tang Xiang Wu Junfeng Feng Qing Mao Guoyi Gao Jiyao Jiang Department of Neurosurgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Institute of Head Trauma Shanghai 200127China

Prognostication of coma patients after brain injury is important, yet challenging. In this study, we evaluated the predictive value of amplitude-integrated electroencephalography （aEEG） for neurological outcomes in coma patients. From January 2013 to January 2016, 128 coma patients after acute brain injury were prospectively enrolled and monitored with aEEG. The 6-month neurological outcome was evaluated using the Cerebral Performance Category Scale. aEEG monitoring commenced at a median of 7.5 days after coma onset. Continuous normal voltage predicted a good 6-month neurological outcome with a sensitivity of 93.6% and specificity of 85.2%. In contrast, continuous extremely low voltage, burst-suppression, or a flat tracing was correlated with poor 6-month neurological outcome with a sensitivity of 76.5% and specificity of 100%. In conclusion, aEEG is a promising predictor of 6-month neurological outcome for coma patients after acute brain injury.

关键词： Amplitude-integrated electroencephalography -Coma Acute brain injury Outcome

Combining Ability and Parent-Offspring Correlation of Maize(Zea may L.) Grain β-Carotene Content with a Complete Diallel

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Journal of Integrative Agriculture 2013年第1期12卷 19-26页

作者： LI Run XIAO Lan-hai WANG Jing LUYan-li RONG Ting-zhao PAN Guang-tang WU Yuan-qi tang qilin LAN Hai CAO Mo-ju Maize Research Institute Sichuan Agricultural University/Key Laboratory of Crop Genetic Resource and Impr ovemen tMinistry of Education/Key Laboratory of Maize Biology and Genetic Breeding on SouthwestMinistry of Agriculture

Vitamin A deficiency has become a worldwide *** foods can potentially be an inexpensive,locally adaptable,and long-term solution to dietary-nutrient *** order to improve the β-carotene content in maize grain by breeding and minimize vitamin A deficiency,a complete diallel cross was designed with eight inbred lines of maize,and 64 combinations were obtained in this *** experimental combinations were planted in Yunnan and Sichuan provinces,respectively,with a random complete block *** β-carotene contents in the grains of the experimental materials were analyzed by high-performance liquid *** the tested materials,the effect difference of general combining ability of the β-carotene content was significant;however,the effect difference of the special combining ability and the reciprocal effect were not *** β-carotene content of maize grain was not influenced significantly by the cross and the reciprocal *** was a significant correlation about the β-carotene content in the maize grains between the F 1 and their *** combinations with high β-carotene content were obviously influenced by the environment,and the mean value of β-carotene content for the experimental materials planted in Ya＇an of Sichuan was higher than that planted in Yuanjiang of Yunnan,with the results being significant at the 0.01 level.

关键词： maize β-carotene content complete diallel cross combining ability

Comprehensive Proteomic Profiling of Patients'Tears Identifies Potential Biomarkers for the Traumatic Vegetative State

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Neuroscience Bulletin 2018年第4期34卷 626-638页

作者： qilin tang Chao Zhang Xiang Wu Wenbin Duan Weiji Weng Junfeng Feng Qing Mao Shubin Chen Jiyao Jiang Guoyi Gao Department of Neurosurgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Head Trauma Institute Shanghai 200127China Department of Neurosurgery Baoshan People's HospitalBaoshan 678000China Department of Neurology Anda HospitalShanghai 201204China

The vegetative state is a complex condition with unclear mechanisms and limited diagnostic, prognostic, and therapeutic methods. In this study, we aimed to explore the proteomic profile of tears from patients in a traumatic vegetative state and identify potential diagnostic markers using tears-a body fluid that can be collected noninvasively. Using iTRAQ quantitative proteomic technology, in the discovery phase, tear samples collected from 16 patients in a traumatic vegetative state and 16 normal individuals were analyzed. Among 1080 identified tear proteins, 57 were upregulated and 15 were downregulated in the patients compared to the controls. Bioinformatics analysis revealed that the differentially-expressed proteins were mainly involved in the wound response and immune response signaling pathways. Furthermore, we verified the levels of 7 differentially-expressed proteins in tears from 50 traumatic vegetative state patients and 50 normal controls （including the samples used in the discovery phase） using ELISA. The results showed that this 7-protein panel had a high discrimination ability for traumatic vegetative state （area under the curve = 0.999）. In sum- mary, the altered tear proteomic profile identified in this study provides a basis for potential tear protein markers for diagnosis and prognosis of the traumatic vegetative state and also provides novel insights into the mechanisms of traumatic vegetative state.

关键词： Trauma Vegetative state - Tears - Proteomics iTRAQ

规范化呼吸道管理在早期非小细胞肺癌根治术中的应用

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现代科学仪器 2024年第2期41卷 82-85页

作者：费贤芹汪海燕陈华汤琪琳陈娟谢慧慧周德存安徽省第二人民医院胸心外科安徽合肥230041

目的:探讨规范化呼吸道管理在早期非小细胞肺癌根治术中的应用效果。方法:选取安徽省第二人民医院胸心外科2020年4月~2022年12月收治的80例行肺癌根治术的早期非小细胞肺癌患者,利用掷硬币法分为两组各40例。对照组实施常规围术期干预,... 详细信息

目的:探讨规范化呼吸道管理在早期非小细胞肺癌根治术中的应用效果。方法:选取安徽省第二人民医院胸心外科2020年4月~2022年12月收治的80例行肺癌根治术的早期非小细胞肺癌患者,利用掷硬币法分为两组各40例。对照组实施常规围术期干预,观察组围术期干预中实施规范化呼吸道管理,比较两组护理效果差异。结果:两组围手术期指标相近(P>0.05);观察组并发症发生率与干预满意度、干预后的血气指标、炎性细胞因子、生命体征指标、肺功能指标优于对照组(P<0.05)。结论:规范化呼吸道管理值得推广。

关键词：早期非小细胞肺癌围术期干预规范化呼吸道管理肺功能

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基于二分K-means理论的织机了机预测

纺织学报 2023年第5期44卷 112-118页

作者：彭来湖唐麒麟戴宁胡旭东浙江理工大学浙江省现代纺织装备技术重点实验室浙江杭州310018 浙江理工大学纺织科学与工程学院(国际丝绸学院) 浙江杭州310018

织布车间内各机台的排产方案与纺织企业生产效率密切相关,为解决现有织机了机时间主要依靠人工经验且难预测导致的排产不及时、不合理等问题,提出一种基于K均值理论的了机预测算法,通过建立织机了机预测理论模型把喷气织机生产全过程合... 详细信息

织布车间内各机台的排产方案与纺织企业生产效率密切相关,为解决现有织机了机时间主要依靠人工经验且难预测导致的排产不及时、不合理等问题,提出一种基于K均值理论的了机预测算法,通过建立织机了机预测理论模型把喷气织机生产全过程合理分为5个生产状态,并按时间序列记录车间内织机生产过程的实时生产状态信息等数据,最后通过Python内置数学处理模块进行求解。结果表明:预测了机时间及实际了机时间之间误差较小,证明了机预测理论模型的正确性,且最大绝对误差不超过2 h,满足织布车间排产所需的时效性及准确性要求。此外,该了机预测模型对具有相似工序的喷水、剑杆等纺机设备同样适用,具有工程应用价值。

关键词：织机排产了机预测二分K-means理论织造工艺

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基于混沌电路的高精度重量测量系统设计

仪器仪表学报 2009年第6期30卷 1202-1206页

作者：杜柳青余永维唐其林重庆工学院汽车学院重庆市汽车零部件及其检测技术重点实验室重庆400050

提出一种基于参数敏感性的混沌测量电路,应用于重量测量系统。其特点是直接将重量映射为二进制代码,从而避开了放大器和A/D转换器等中间环节对重量测量精度的制约和影响,大大提高了重量测量系统的精度和分辨率。分析了混沌重量测量原理... 详细信息

提出一种基于参数敏感性的混沌测量电路,应用于重量测量系统。其特点是直接将重量映射为二进制代码,从而避开了放大器和A/D转换器等中间环节对重量测量精度的制约和影响,大大提高了重量测量系统的精度和分辨率。分析了混沌重量测量原理,研究了相应的混沌电路实现方法,设计了以DSP作为控制器的混沌重量测量系统。理论计算和实验数据都表明,该重量测量系统精度高,方法可行。

关键词：参数敏感性混沌电路重量测量精度

审美益智创造塑形——公共舞蹈艺术的教育价值探析

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湖南工业大学学报（社会科学版） 2014年第5期19卷 122-124页

作者：汤其林湖南工业大学音乐学院湖南株洲412000

公共舞蹈艺术在大学教育中的特殊价值,显现了艺术教育以人为本的关键,揭示出了教育的根本意义。公共舞蹈教育可以提升大学生的审美能力,促进大学生多元智能的发展,提高大学生的创造力,塑造大学生的健美形象。

关键词：公共舞蹈艺术教育价值审美创造