检索结果-南通市图书馆

Biomedical and Environmental Sciences 2012年第3期25卷 282-290页

作者： GU Wen Peng WANG Xin QIU Hai Yan LUO Xia XIAO Yu Chun tang liu ying KAN Biao XU Jian Guo JING Huai Qi National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionState Key Laboratory Jbr Infectious Disease Prevention and Control Beijing 102206China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective Yersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system. We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y. enterocolitico. Methods We used SDS-PAGE and western blotting to characterize lipopolysaccharide （LPS）, Yersinio outer membrane proteins （Yops）, membrane proteins, and whole-cell proteins from poorly pathogenic Y. enterocolitico bio-serotype 2/0：9, isolated from China, and highly pathogenic bio-serotype 1B/O：8, isolated from Japan. Results These two strains of Y. enterocolitica had different LPS immune response patterns. Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity. The membrane and whole-cell proteins of both strains were similar; immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains. Conclusion The major antigens of the two strains e and membrane proteins, as shown by comparing preparations. citing the host immune protein samples with response were the LPS reference and purified

关键词： Immunogens Lipopolysaccharides Outer membrane proteins Whole-cell proteins Pathogenic Yersinia enterocolitica

Human bocavirus in children suffering from acute lower respiratory tract infection in Beijing Children＇s Hospital

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2008年第17期121卷 1607-1610页

作者： ZHANG Li-li tang liu-ying XIE Zheng-de TAN Xiao-juan LI Chong-shan CUI Ai-li JI Yi-xin XU Song-tao MAO Nai-ying XU Wen-bo SHEN Kun-ling Virus Laboratory Beijing Children's Hospital Capital Medical University Beijing 100045 China Shanghai Municipal Centre for Disease Control and Prevention Shanghai 200336 China Shanghai Municipal Centre for Disease Control and Prevention Shanghai 200336 China

Background Human bocavirus （HBoV） is a parvovirus recently found to possibly cause respiratory tract disease in children and adults. This study investigated HBoV infection and its clinical characteristics in children younger than five years of age suffering from acute lower respiratory tract infection in Beijing Children＇s Hospital. Methods Nasopharyngeal aspirates were collected from children suffering from acute lower respiratory tract infection during the winters of 2004 to 2006 （from November through the following February）. HBoV was detected by polymerase chain reaction amplification and virus isolation and the amplification products were sequenced for identification. Results HBoV infection was detected in 16 of 333 study subjects. Coinfections with respiratory syncytial virus were detected in 3 of 16 HBoV positive patients with acute lower respiratory tract infection. The median age for HBoV positive children was 8 months （mean age, 17 months; range, 3 to 57 months）. Among the HBoV positive children, 14 were younger than 3 years old, 9 were younger than 1 year old and 7 were younger than 6 months. These 16 positive HBoV children exhibited coughing and abnormal chest radiography findings and more than 60% of these children had wheezing and fever. Ten children were clinically diagnosed with pneumonia, 2 bronchiolitis, 2 acute bronchitis and 2 asthma. One child died. Conclusions HBoV was detected in about 5% of children with acute lower respiratory infection seen in Beijing Children＇s HosPital. Further investigations regarding clinical and epidemiologic characteristics of HBoV infection are needed.

关键词： Bocavirus child respiratory tract infections polymerase chain reaction

Efficacy and safety of remimazolam in bronchoscopic sedation:A meta-analysis

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第6期12卷 1120-1129页

作者： ying Zhou Cheng Zhao Yi-Xun tang Ji-Tong liu Department of Anesthesiology The First Affiliated Hospital of Hunan Normal University(Hunan Provincial People’s Hospital)Changsha 410005Hunan ProvinceChina Department of Anesthesiology Zhangjiajie People's HospitalZhangjiajie 427000Hunan ProvinceChina

BACKGROUND Remimazolam is a new benzodiazepine used for procedural sedation and general anesthesia.Several studies have used remimazolam for bendable bronchoscopy.AIM To assess the safety and efficacy of remimazolam for sedation in patients undergoing bendable bronchoscopy by performing a meta-analysis of randomized controlled trials(RCTs).METHODS We searched the EMBASE,PubMed,Cochrane Library,and Web of Science databases for RCTs on bendable bronchoscopic procedural sedation with remimazolam vs conventional sedatives(CS).RESULTS Five studies with 1080 cases were included.Remimazolam had the same sedation success rate compared with CS[relative risk(RR):1.35,95%CI:0.60-3.05,P=0.474,I2=99.6%].However,remimazolam was associated with a lower incidence of hypotension(RR:0.61;95%CI:0.40-0.95,P=0.027;I2=65.1%)and a lower incidence of respiratory depression(RR:0.50,95%CI:0.33-0.77,P=0.002,I2=42.3%).A subgroup analysis showed a higher success rate of sedation with remimazolam than midazolam(RR:2.45,95%CI:1.76-3.42,P<0.001).Compared with propofol,the incidence of hypotension(RR:0.45,95%CI:0.32-0.64,P<0.001,I2=0.0%),respiratory depression(RR:0.48,95%CI:0.30-0.76,P=0.002,I2=78.4%),hypoxemia(RR:0.36,95%CI:0.15-0.87,P=0.023),and injection pain(RR:0.04,95%CI:0.01-0.28,P=0.001)were lower.CONCLUSION Remimazolam is safe and effective during bronchoscopy.The sedation success rate was similar to that in the CS group.However,remimazolam has a higher safety profile,with fewer inhibitory effects on respiration and circulation.

关键词： Remimazolam Bronchoscopy Procedural sedation Meta-analysis

Ti_(3)C_(2)T_(x) MXene/carbon composites for advanced supercapacitors:Synthesis,progress,and perspectives

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Carbon Energy 2024年第2期6卷 113-142页

作者： Yanqing Cai Xinggang Chen ying Xu Yalin Zhang Huijun liu Hongjuan Zhang Jing tang College of Materials Science and Engineering North China University of Science and TechnologyTangshanChina Hebei Iron and Steel Laboratory North China University of Science and TechnologyTangshanChina Songshan Lake Materials Laboratory DongguanChina Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghaiChina State Key Laboratory of Petroleum Molecular and Process Engineering(SKLPMPE) Sinopec Research Institute of Petroleum Processing Co.Ltd.BeijingChina Institute of Eco-Chongming ShanghaiChina

MXenes are a family of two-dimensional(2D)layered transition metal carbides/nitrides that show promising potential for energy storage applications due to their high-specific surface areas,excellent electron conductivity,good hydrophilicity,and tunable terminations.Among various types of MXenes,Ti_(3)C_(2)T_(x) is the most widely studied for use in capacitive energy storage applications,especially in supercapacitors(SCs).However,the stacking and oxidation of MXene sheets inevitably lead to a significant loss of electrochemically active sites.To overcome such challenges,carbon materials are frequently incorporated into MXenes to enhance their electrochemical properties.This review introduces the common strategies used for synthesizing Ti_(3)C_(2)T_(x),followed by a comprehensive overview of recent developments in Ti_(3)C_(2)T_(x)/carbon composites as electrode materials for SCs.Ti_(3)C_(2)T_(x)/carbon composites are categorized based on the dimensions of carbons,including 0D carbon dots,1D carbon nanotubes and fibers,2D graphene,and 3D carbon materials(activated carbon,polymer-derived carbon,etc.).Finally,this review also provides a perspective on developing novel MXenes/carbon composites as electrodes for application in SCs.

关键词： electrochemical performance MXene/carbon composites supercapacitors

Influence of powder oxidation on powder properties and formability in H13 hot-work steels processed by electron beam melting

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research(International) 2024年第4期31卷 924-932页

作者： Wei liu Yan Wang Li-xiong Han ying-kang Wei Hui-ping tang Shi-feng liu School of Metallurgical Engineering Xi'an University of Architecture and TechnologyXi'an 710055ShaanxiChina School of Engineering Zhejiang University City CollegeHangzhou 310015ZhejiangChina

The oxygen content of metal powder is decisive for the recyclability of powder.The research on the effect of oxygen content on powder properties and material formability has practical significance for economical production with additive manufacturing while preventing the waste of resources.Here,we deliberately oxidized the powder by baking at high temperature to increase the oxygen content in the powder and gave the calculation method of the oxygen content in the powder oxidation film.The majority of oxygen element was found in the oxide particles in the powder and the oxide flm on the powder surface,which did affect the flowability of the powder.It is worth noting that the increase in the oxygen content does not change the phase of H13 steel,but it can promote the molten pool flow and obtain a smoother surface.The increase in the oxygen content in the powder is not the decisive factor for the formability and defects of the printed samples.It is the combined effect of the powder deformation,the increase in the oxygen content,and the impurity pollution after repeated use,which leads to the limitation of repeated utilization of the powder.

关键词： Additive manufacturing Electron beam melting H13 steel powder-Powder oxidation

Identification of anti-gastric cancer effects and molecular mechanisms of resveratrol: From network pharmacology and bioinformatics to experimental validation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2024年第2期16卷 493-513页

作者： ying-Qian Ma Ming Zhang Zhen-Hua Sun Hong-Yue tang ying Wang Jiang-Xue liu Zhan-Xue Zhang Chao Wang Department of Oncology Hebei General HospitalShijiazhuang 050051Hebei ProvinceChina School of Graduate Studies Hebei Medical UniversityShijiazhuang 050017Hebei ProvinceChina Clinical Medical Research Center Hebei General HospitalShijiazhuang 050051Hebei ProvinceChina Department of Gastrointestinal Surgery Second Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina

BACKGROUND Gastric cancer(GC)is one of the most aggressive malignancies with limited therapeutic options and a poor prognosis.Resveratrol,a non-flavonoid poly-phenolic compound found in a variety of Chinese medicinal materials,has shown excellent anti-GC effect.However,its exact mechanisms of action in GC have not been clarified.AIM To identify the effects of resveratrol on GC progression and explore the related molecular mechanisms.METHODS Action targets of resveratrol and GC-related targets were screened from public databases.The overlapping targets between the two were confirmed using a Venn diagram,and a“Resveratrol-Target-GC”network was constructed using Cyto-scape software version 3.9.1.The protein-protein interaction(PPI)network was constructed using STRING database and core targets were identified by PPI network analysis.The Database for Annotation,Visualization and Integrated A total of 378 resveratrol action targets and 2154 GC disease targets were obtained from public databases,and 181 intersection targets between the two were screened by Venn diagram.The top 20 core targets were identified by PPI network analysis of the overlapping targets.GO function analysis mainly involved protein binding,identical protein binding,cytoplasm,nucleus,negative regulation of apoptotic process and response to xenobiotic stimulus.KEGG enrichment analysis suggested that the involved signaling pathways mainly included PI3K-AKT signaling pathway,MAPK signaling pathway,IL-17 signaling pathway,TNF signaling pathway,ErbB signaling pathway,etc.FBJ murine osteosarcoma viral oncogene homolog(FOS)and matrix metallopeptidase 9(MMP9)were selected by differential expression analysis,and they were closely associated with immune infiltration.Molecular docking results showed that resveratrol docked well with these two targets.Resveratrol treatment arrested the cell cycle at the S phase,induced apoptosis,and weakened viability,migration and invasion in a dose-dependent manner.Furthermore,resveratrol could exhibit anti-GC effect by regula

关键词： Resveratrol Gastric cancer Network pharmacology Bioinformatics Molecular docking

Semisynthesis of homogeneous spike RBD glycoforms from SARS-CoV-2 for profiling the correlations between glycan composition and function

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2024年第2期11卷 145-154页

作者： Farong Ye Cheng Li Feng-Liang liu Xinliang liu Peng Xu Rong-Hua Luo Wenping Song Yong-tang Zheng Tianlei ying Biao Yu Ping Wang Center for Chemical Glycobiology Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study School of Chemistry and Chemical Engineering Shanghai Jiao Tong University MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection Shanghai Institute of Infectious Disease and BiosecurityShanghai Engineering Research Center for Synthetic ImmunologySchool of Basic Medical Sciences Shanghai Medical College Fudan University Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province Center for Biosafety Mega-Science Kunming Institute of ZoologyChinese Academy of Sciences State Key Laboratory of Bioorganic and Natural Products Chemistry Center for Excellence in Molecular Synthesis Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shenzhen Research Institute of Shanghai Jiao Tong University

Vaccines have been the primary remedy in the global fight against coronavirus disease 2019（COVID-19）.The receptor-binding domain（RBD） of the spike protein, a critical viral immunogen, is affected by the heterogeneity of its glycan structures and relatively low immunogenicity. Here, we describe a scalable synthetic platform that enables the precise synthesis of homogeneously glycosylated RBD, facilitating the elucidation of carbohydrate structure–function relationships. Five homogeneously glycosylated RBDs bearing biantennary glycans were prepared, three of which were conjugated to T-helper epitope(Tpep) from tetanus toxoid to improve their weak immune response. Relative to natural HEK293-derived RBD, synthetic RBDs with biantennary N-glycan elicited a higher level of neutralising antibodies against SARS-CoV-2 in mice. Furthermore, RBDs containing Tpepelicited significant immune responses in transgenic mice expressing human angiotensin-converting enzyme 2. Our collective data suggest that trimming the N-glycans and Tpepconjugation of RBD could potentially serve as an effective strategy for developing subunit vaccines providing efficient protection.

关键词： peptide ligation glycosylation glycoprotein

Flexible and Robust Functionalized Boron Nitride/Poly(p‑Phenylene Benzobisoxazole)Nanocomposite Paper with High Thermal Conductivity and Outstanding Electrical Insulation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2024年第2期16卷 423-437页

作者： Lin tang Kunpeng Ruan Xi liu Yusheng tang Yali Zhang Junwei Gu Chongqing Key Laboratory of Green Synthesis and Applications College of ChemistryChongqing Normal UniversityChongqing 401331People’s Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’an 710072People’s Republic of China

With the rapid development of 5G information technology,thermal conductivity/dissipation problems of highly integrated electronic devices and electrical equipment are becoming prominent.In this work,“high-temperature solid-phase&diazonium salt decomposition”method is carried out to prepare benzidine-functionalized boron nitride(m-BN).Subsequently,m-BN/poly(pphenylene benzobisoxazole)nanofiber(PNF)nanocomposite paper with nacremimetic layered structures is prepared via sol–gel film transformation approach.The obtained m-BN/PNF nanocomposite paper with 50 wt%m-BN presents excellent thermal conductivity,incredible electrical insulation,outstanding mechanical properties and thermal stability,due to the construction of extensive hydrogen bonds andπ–πinteractions between m-BN and PNF,and stable nacre-mimetic layered structures.Itsλ∥andλ_(⊥)are 9.68 and 0.84 W m^(-1)K^(-1),and the volume resistivity and breakdown strength are as high as 2.3×10^(15)Ωcm and 324.2 kV mm^(-1),respectively.Besides,it also presents extremely high tensile strength of 193.6 MPa and thermal decomposition temperature of 640°C,showing a broad application prospect in high-end thermal management fields such as electronic devices and electrical equipment.

关键词： Poly(p-phenylene-2,6-benzobisoxazole)nanofiber Boron nitride Thermal conductivity Electrical insulation

The Investigation of Data Voting Algorithm for Train Air-Braking System Based on Multi-Classification SVM and ANFIS

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Electronics 2024年第1期33卷 274-281页

作者： Juhan WANG ying GAO Yuan CAO Tao tang School of Electronics and Information Engineering Beijing Jiaotong University Graduate Department China Academy of Railway Science National Engineering Research Center of Rail Transportation Operation and Control System Beijing Jiaotong University

The pressure data of the train air braking system is of great significance to accurately evaluate its operation state. In order to overcome the influence of sensor fault on the pressure data of train air braking system, it is necessary to design a set of sensor fault-tolerant voting mechanism to ensure that in the case of a pressure sensor fault, the system can accurately identify and locate the position of the faulty sensor, and estimate the fault data according to other normal data. A fault-tolerant mechanism based on multi-classification support vector machine(SVM) and adaptive network-based fuzzy inference system(ANFIS) is introduced. Multi-classification SVM is used to identify and locate the system fault state, and ANFIS is used to estimate the real data of the fault sensor. After estimation, the system will compare the real data of the fault sensor with the ANFIS estimated data. If it is similar,the system will recognize that there is a false alarm and record it. Then the paper tests the whole mechanism based on the real data. The test shows that the system can identify the fault samples and reduce the occurrence of false alarms.

关键词： Multi-classification support vector machine Adaptive network-based fuzzy inference system Train air braking system Fault-tolerant voting Multi-sensors

Quantification of perfluorinated compounds in atmospheric particulate shows potential connection with environmental event

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第2期136卷 237-247页

作者： Hua tang ying Wang Shengling Si Hongli Li David Da Yong Chen National Institute of Metrology 18 BeisanhuandongluChaoyang DistrictBeijing 100029China School of Chemistry and Materials Science Nanjing Normal UniversityNanjing 210023China Si Chuan Zhong Ce Biao Wu Technology Co.Ltd. Sichuan 610052China Department of Chemistry University of British ColumbiaVancouver V6T 1Z1BCCanada

A method of quantification of perfluorinated compounds(PFCs)from atmospheric particulate matter(APM)is described.A single step pretreatment method,selective pressurized liquid extraction(SPLE),was developed to reduce the high matrix background and avoid contamination from commonly used multiple sample pretreatment steps.An effective sorbent was selected to purify the PFCs during SPLE,followed by liquid chromatography-tandem mass spectrometry(LC–MS/MS),for quantification of PFCs.Conditions affecting the SPLE efficiency,including temperature,static extraction time,and number of extraction cycles used,were studied.The optimum conditions were found to be 120℃,10 min,and 3 cycles,respectively.LC-MS/MS method was developed to obtain the optimal sensitivity specific to PFCs.The method detection limits(MDLs)were 0.006 to 0.48 ng/g for the PFCs studied and the linear response range was from 0.1 to 100 ng/g.To ensure accurate values were obtained,each step of the experiment was evaluated and controlled to prevent contamination.The optimized method was tested by performing spiking experiments in natural particulate matter matrices and good rates of recovery and reproducibility were obtained for all target compounds.Finally,the method was successfully used to measure 16 PFCs in the APM samples collected in Beijing over five years from 2015 to 2019.It is observed that some PFCs follow the trend of total PFC changes,and can be attributed to the environment influencing events and policy enforcement,while others don't seem to change as much with time of the year or from year to year.

关键词： Selective pressurized liquid extraction Liquid chromatography-mass spectrometry Perfluorinated compounds Atmospheric particulate matter Pollution control