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Characterization,expression dynamics,and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta

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Journal of Oceanology and Limnology 2024年第1期42卷 187-200页

作者： Xinming GAO Binbin FENG Chen DU Congcong HOU Shan JIN daojun tang Junquan ZHU Key Laboratory of Applied Marine Biotechnology by the Ministry of Education and Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China College of Ecology Lishui UniversityLishui 323000China

Mitochondria undergo morphological changes during spermatogenesis in some *** mechanism and role of mitochondrial morphology regulation,however,remain somewhat *** this study,we analyzed the molecular characteristics,expression dynamics and subcellular localization of optic atrophy protein 1(OPA1),a mitochondrial fusion and cristae maintenance-related protein,to reveal the possible regulatory mechanisms underlying mitochondrial morphology in Phascolosoma esculenta *** full-length cDNA of the *** opa1 gene(Pe-opa1)is 3743 bp in length and encodes 975 amino *** Pe-OPA1 protein is highly conservative and includes a transmembrane domain,a GTPase domain,two helical bundle domains,and a lipid-interacting *** and protein expression was higher in the coelomic fluid(a site of spermatid development)of male *** and increased first and then decreased from March to ***,their expression during the breeding stage was significantly higher than during the non-breeding stage,suggesting that Pe-OPA1 is involved in *** *** Pe-OPA1 protein was more abundant in components consisting of many spermatids than in components without,indicating that Pe-OPA1 mainly plays a role in the spermatid in coelomic ***,Pe-OPA1 was mainly detected in the spermatid *** experiments showed that the Pe-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis,suggesting its involvement in *** *** results provide evidence for Pe-OPA1's involvement in the regulation of mitochondrial morphology during spermiogenesis.

关键词： optic atrophy protein 1(OPA1) spermiogenesis Phascolosoma esculenta mitochondrial dynamics spermatid

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Physiological and histological responses of Phascolosoma esculenta (Sipuncula: Phascolosomatidea) to acute heat stress

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Journal of Oceanology and Limnology 2022年第2期40卷 643-655页

作者： Xinming GAO Haiyan YANG daojun tang Chen DU Shan JIN Congcong HOU Chundan ZHANG Junquan ZHU Jianping WANG Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China Ningbo Academy of Oceanology and Fisheries Ningbo 315012China

Temperature is an important factor affecting the growth,development,and survival of *** effects of temperature stress on aquatic organisms have received increasing attention,as these organisms are mostly poikilotherms and their body temperature are directly corresponding changes with ambient temperature,resulting in they are easily exposed in temperature ***,little is known about the effects of high temperature on *** this study,we investigated the effects of acute heat stress on malondialdehyde(MDA)concentration,the activities of antioxidant(superoxide dismutase and glutathione peroxidase)and immunity-related(acidic and alkaline phosphatase)enzymes,heat shock protein 70(hsp70)and hsp90 gene expression,and the histological structure of the sipunculid Phascolosoma *** the coelom fl uid,the MDA concentration and all detected antioxidant enzyme activities increased during high temperature stress;signifi cant increases were also observed here and in the intestine in the hsp70 and hsp90 mRNA expression *** results indicated that acute heat stress caused oxidative stress;antioxidants and heat shock proteins probably act to protect *** against oxidative damage,constituting part of its physiological mechanism for adaptation to high *** addition,the increased activity of the acidic and alkaline phosphatases indicated eff ects on its nonspecifi c immune ***,damaged tissue structures were observed in the body wall,retractor muscle,intestine,and nephridium after 96 h of 40-℃ *** damaged cells of these tissues showed obvious condensed chromatin around the nuclear *** histological damage suggests that heat stress could affect movement,food absorption,digestion,and excretion in *** results elucidate the effects of temperature stress on *** and its physiological response mechanisms and provide practical indicators for assessing heat stress status and determi

关键词： heat stress oxidation stress enzyme activity heat shock proteins nonspecific immunity histological damage

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Prohibitin Protein Expression During Spermatogenesis in the Large Yellow Croaker, Larimichthys crocea

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Journal of Ocean University of China 2022年第5期21卷 1251-1264页

作者： LIN Chenwen GAO Xinming NI Jie ZHANG Shengshuo LIU Cheng LUO Shengyu DU Chen ZHENG Xuebin HOU Congcong tang daojun ZHANG Chundan ZHU Junquan Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China

Mitochondria are important for animals’fertility,and their morphologies and functions during spermatogenesis are un-der ***,the molecular mechanism that regulates the mitochondrial dynamic during spermatogenesis is still *** this study,the cytological features of spermatogenesis were investigated in Larimichthys *** addition,the structure and function of prohibitin(PHB),which is associated with mitochondrial structure and dynamic,was also *** full-length cDNA and protein(Lc-PHB)from the *** phb gene(Lc-phb)contained 1625 base pairs and 271 amino acids,***-PHB had a conserved primary structure that resulted in a transmembrane,SPFH(the analogous region of proteins stomat-ins,prohibitins,flotillins and HflK/C),and coiled-coil *** was detected at high levels in the muscle,liver,and heart,and at intermediate levels in the testis,gill,and ***-phb mRNA expression was detected in spermatogenic cells by fluorescence in situ *** immunofluorescence assay revealed that PHB protein was localized in the mitochondria during ***,PHB expression was detected in the perinuclear cytoplasm of spermatogonia,spermatocytes,and spermatids in the early developmental stage,and mainly localized on one side of the nuclei in the cytoplasm of spermatids in a middle developmental stage,and finally on the sperm *** blotting showed that PHB was located in the extracted mitochondria protein fraction but not in the cytoplasm protein fraction of ***,these results indicated that PHB was expressed in the mitochondria dur-ing *** addition,the study explained the mitochondrial dynamic during fish spermatogenesis and proposed a possi-ble relationship among PHB,spermatogenesis,and male fertility.

关键词： spermatogenesis ultrastructure prohibitin expression pattern subcellular localization

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Molecular Characterization, Tissue Distribution and Localization of Larimichthys crocea Kif3a and Kif3b and Expression Analysis of Their Genes During Spermiogenesis

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Journal of Ocean University of China 2019年第6期18卷 1451-1469页

作者： MU Danli DU Chen FU Suyan WANG Jingqian HOU Congcong tang daojun ZHU Junquan The Key Laboratory of Applied Marine Biotechnology of Ministry of Education School of Marine SciencesNingbo University

KIF3A and KIF3B are two N-terminal motor proteins belonging to the kinesin-II superfamily that play essential roles in *** understand the roles played by KIF3 A/3B during spermatogenesis of large yellow croaker Larimichthys crocea,we studied the testis characteristics at different developmental stages of ***,and determined the spatiotemporal expression patterns of kif3a and kif3b during *** real-time PCR(qR T-PCR)showed that the overall trends of kif3 a/3 b m RNA abundance during testis development are *** stage Ⅱ to stage V,kif3a/3b m RNA abundances first increased and then fell after reaching a peak at stage ***,the m RNA abundances of both genes at stage V were higher than those at stages Ⅱ and Ⅲ.In addition,it is worth of noting that kif3 b m RNA abundance was higher than that of kif3a at all *** in situ hybridization results revealed that kif3a/3b m RNA abundance dynamics were consistent with the migration of mitochondria,the deformation of nucleus,and the formation of *** m RNA hybridization signals of both genes first appeared either around the nuclear periphery or on the side of the nuclei,then appeared at one side of nuclei,and finally were mainly on the tail during *** findings contributed to better understanding the molecular mechanisms of spermiogenesis in fish;and suggested that KIF3A and KIF3B may participate in the intracellular transport of mitochondria,nuclear deformation,and the formation of tail during the spermiogenesis in ***.

关键词： Larimichthys crocea kif3a/3b spermiogenesis expression pattern

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计及高比例新能源多种控制方式及运行约束的连续潮流

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电力系统自动化 2024年第16期48卷 123-131页

作者：赵晋泉汤奕党杰崔挺陈道君教育部海上风电技术工程研究中心(上海电力大学) 上海市200090 国家电网有限公司华中分部湖北省武汉市430077 国网湖南省电力有限公司电力科学研究院湖南省长沙市410208

高比例新能源电网的电压稳定问题突出,连续潮流是电压稳定分析的重要工具。现有含电压源换流器(VSC)电源电网的连续潮流未充分考虑VSC的多种控制方式以及运行约束,导致电压稳定裕度计算的准确性不足。文中提出同时计及新能源并网VSC的... 详细信息

高比例新能源电网的电压稳定问题突出,连续潮流是电压稳定分析的重要工具。现有含电压源换流器(VSC)电源电网的连续潮流未充分考虑VSC的多种控制方式以及运行约束,导致电压稳定裕度计算的准确性不足。文中提出同时计及新能源并网VSC的输出电流约束和内电势约束,以及考虑构网型新能源恒电压控制、无功功率-电压下垂控制和跟网型新能源恒无功控制的连续潮流模型和计算方法,给出了由约束导致的控制方式切换逻辑和分岔点类型识别方法。通过改造的IEEE 39节点系统算例结果表明,所提模型与算法提升了传统连续潮流方法对高比例新能源电网静态电压稳定分析的适用性。

关键词：高比例新能源电压稳定性潮流电压源换流器切换逻辑控制方式

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农业推广硕士培养目标的实现与学位论文管理

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科教文汇 2015年第8期 130-132页

作者：唐道军尹萍宁波大学浙江宁波315211

在农业推广硕士培养过程中,高质量的学位论文是实现其培养目标的重要保障。本文结合实际,重点阐述了农业推广硕士学位论文管理中存在的主要问题及高质量学位论文管理的重要保障措施,以期为今后农业推广硕士学位论文管理提供依据和参考。

关键词：农业推广硕士培养学位管理

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光合作用抑制剂DCMU对三角褐指藻岩藻黄素含量和叶绿素荧光特征及关键基因表达的影响

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核农学报 2020年第8期34卷 1705-1712页

作者：郑小恽龚一富李申睿方清姝王何瑜唐道军宁波大学海洋学院浙江宁波315832 宁波大学食品与药学学院浙江宁波315832

为了阐明岩藻黄素生物合成与光合作用之间的关系,研究不同浓度光合作用抑制剂二氯苯基二甲脲(DCMU)对三角褐指藻细胞密度、岩藻黄素含量、叶绿素含量、叶绿素荧光特征和相关关键基因表达的影响。结果表明,不同浓度DCMU均抑制细胞生长,... 详细信息

为了阐明岩藻黄素生物合成与光合作用之间的关系,研究不同浓度光合作用抑制剂二氯苯基二甲脲(DCMU)对三角褐指藻细胞密度、岩藻黄素含量、叶绿素含量、叶绿素荧光特征和相关关键基因表达的影响。结果表明,不同浓度DCMU均抑制细胞生长,使三角褐指藻内岩藻黄素含量降低,叶绿素含量增加;当DCMU浓度为1 mg·L^-1时,三角褐指藻内岩藻黄素含量最少,较CK减少了26.98%,而叶绿素含量最高(3.16 mg·g^-1)。随着DCMU浓度的增加,电子传递的抑制作用增强,实际光化学量子效率[Y(Ⅱ)]、 rETR、相对电子传递速率(NPQ)、光化学淬灭系数(qP)和快速光曲线初始斜率(α)均明显降低,DCMU为1 mg·L^-1时出现电子完全阻断的现象。定量PCR分析结果表明,岩藻黄素合成通路关键基因pds、lcyb的表达水平与岩藻黄素含量变化趋势一致。相关性分析表明,岩藻黄素含量与Y(Ⅱ)、qP和α之间存在显著正相关(P<0.05),说明三角褐指藻岩藻黄素的生物合成不仅与岩藻黄素合成关键基因的表达有关,还与光合作用有关。本研究为进一步探明三角褐指藻岩藻黄素合成的生理生化和分子机理提供了参考。

关键词： DCMU 三角褐指藻岩藻黄素叶绿素荧光特征基因表达

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非标记定量蛋白质组学分析冻结方式对中华管鞭虾肌肉差异蛋白质的影响

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核农学报 2020年第12期34卷 2681-2691页

作者：石娟李勇勇周丽萍李超群王晴唐道军娄永江宁波大学食品与药学学院浙江宁波315211 宁波大学海洋学院浙江宁波315211

为了探究冻结方式对中华管鞭虾(Solenocera melantho)肌肉差异蛋白质的影响,本试验运用非标记(label-free)定量蛋白质组学技术,研究液体浸渍冻结、真空包装结合浸渍冻结、静止空气冻结、真空包装结合空气冻结4种方式对中华管鞭虾差异蛋... 详细信息

为了探究冻结方式对中华管鞭虾(Solenocera melantho)肌肉差异蛋白质的影响,本试验运用非标记(label-free)定量蛋白质组学技术,研究液体浸渍冻结、真空包装结合浸渍冻结、静止空气冻结、真空包装结合空气冻结4种方式对中华管鞭虾差异蛋白表达的影响。结果表明,与新鲜中华管鞭虾相比,上述4种冻结方式处理后的虾肌肉中分别有269、162、299、289个差异蛋白,其中表达上调的蛋白分别有100、59、63、61个,表达下调的蛋白分别有169、103、236、228个。筛选出甘油-3-磷酸脱氢酶(NAD^+)、β-胸腺素2个差异蛋白,有望成为与冻结中华管鞭虾品质变化相关的指示蛋白标志物。本研究为深入了解中华管鞭虾在不同冻结方式下的品质变化机制,以及寻找出与新鲜度相关的指示蛋白提供了理论依据,也为虾的冻结工艺技术提供了参考。

关键词：非标记定量蛋白质组学中华管鞭虾冻结方式差异蛋白生物信息学分析

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GC-MS结合自动解卷积技术分析谷壳中挥发性风味成分

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中国酿造 2024年第2期43卷 243-252页

作者：毕荣宇王国江卢君唐平山其木格王丽王凡李长文王道军陆安谋贵州国台酒业集团股份有限公司贵州仁怀564501 贵州国台酒业集团研究院天津300410

为了探索酱香型白酒酿造用辅料谷壳中挥发性风味成分的组成,建立了顶空固相微萃取(HS-SPME)-气相色谱-质谱联用(GC-MS)结合自动解卷积系统(AMDIS)和保留指数(RI)对谷壳中的化学成分进行鉴定分析的方法,并采用正交试验优化HS-SPME参数条... 详细信息

为了探索酱香型白酒酿造用辅料谷壳中挥发性风味成分的组成,建立了顶空固相微萃取(HS-SPME)-气相色谱-质谱联用(GC-MS)结合自动解卷积系统(AMDIS)和保留指数(RI)对谷壳中的化学成分进行鉴定分析的方法,并采用正交试验优化HS-SPME参数条件。结果表明,最佳的萃取条件为样品质量10 g,萃取温度60℃,萃取时间50 min。谷壳中共鉴定出挥发性物质173种,其中醇类17种(10.06%)、醛类23种(15.28%)、酸类11种(40.26%)、酯类6种(2.00%)、酮类21种(4.22%)、呋喃类7种(1.34%)、呋喃酮类1种(0.15%)、吡嗪类3种(0.14%)、内酯类6种(1.95%)、芳香族44种(11.44%)、烃类21种(4.43%)、杂环类7种(1.15%)、含硫化合物1种(0.04%)、萜烯类5种(7.54%)。

关键词：顶空固相微萃取气相色谱-质谱法自动解卷积系统保留指数谷壳挥发性风味成分

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银鲳卵巢发育与磷酸酶ACP及AKP活性的关系

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宁波大学学报（理工版） 2020年第6期33卷 13-19页

作者：刘梦迪周楠楠王腾飞徐善良唐道军宁波大学海洋学院浙江宁波315832 宁波大学应用海洋生物技术教育部重点实验室浙江宁波315832

为了探究人工养殖银鲳(Pampusargenteus)卵巢发育成熟过程中相关磷酸酶的变化规律,采用组织切片的方法系统描述了各期卵巢的形态特征及其组织学结构变化,并分析了其卵巢、肝脏和肌肉中酸性磷酸酶(ACP)和碱性磷酸酶(AKP)的活性.结果表明:... 详细信息

为了探究人工养殖银鲳(Pampusargenteus)卵巢发育成熟过程中相关磷酸酶的变化规律,采用组织切片的方法系统描述了各期卵巢的形态特征及其组织学结构变化,并分析了其卵巢、肝脏和肌肉中酸性磷酸酶(ACP)和碱性磷酸酶(AKP)的活性.结果表明:(1)银鲳卵巢在Ⅱ期末出现嗜碱性的卵黄核,第3时相卵母细胞出现卵黄颗粒沉积现象;至第4时相卵黄颗粒明显增多,脂滴出现,直至充满整个卵母细胞,核开始偏位;第5时相卵子内卵黄颗粒已融合成块状.(2)随性腺发育,卵巢、肝脏和肌肉中ACP和AKP活性均表现出显著变化(P肌肉>肝脏,在卵巢中ACP活性变化幅度为18.742~73.381 U;AKP为41.272~63.370 U.(3)肌肉中的ACP和AKP以及肝脏中的AKP活性从Ⅰ期至Ⅵ期均呈现下降趋势,而卵巢中的ACP与AKP酶活性则先升后降.研究认为3种组织中ACP和AKP活性变化与银鲳卵巢发育中卵黄积累显著相关(P<0.05).

关键词：银鲳卵巢发育酸性磷酸酶(ACP) 碱性磷酸酶(AKP)

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