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数字化PCR芯片技术用于食源性细菌多重定量检测

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分析化学 2024年第6期52卷 789-798页

作者：茅珍珍胡文琪唐曲蒋汶君秦玉岭吴丽南通大学公共卫生学院南通市公共卫生与医学分析重点实验室南通大学医学院

食源性细菌可造成食物腐败变质并产生多种有害毒素，严重危害食品安全和人体健康。因此，开发食源性细菌有效的检测方法具有十分重要的意义。本研究提出了基于多重数字化聚合酶链式反应（PCR）芯片的分析方法，可对3种常见食源性细菌（... 详细信息

食源性细菌可造成食物腐败变质并产生多种有害毒素，严重危害食品安全和人体健康。因此，开发食源性细菌有效的检测方法具有十分重要的意义。本研究提出了基于多重数字化聚合酶链式反应（PCR）芯片的分析方法，可对3种常见食源性细菌（金黄色葡萄球菌（Staphylococcus aureus）、沙门氏菌（Salmonella typhimurium）和单增李斯特菌（Listeria monocytogenes））进行快速且高效的检测。此芯片采用离心力驱动的数字化进样方式，15 min内即可完成数字化检测过程，操作简便。PCR实验结果表明，经历25个热循环后即可检出阳性信号。此芯片在低浓度细菌核酸定量检测方面具有显著优势，可有效避免假阴性结果。利用此芯片可对浓度为10～5～10～8CFU/mL的细菌样本进行多重检测。相比荧光定量PCR技术，此芯片在分析低含量靶标时具有更高的准确度和灵敏度。

关键词：数字化芯片多重聚合酶链式反应金黄色葡萄球菌沙门氏菌单增李斯特菌

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Fresh Processing Technology in Polygonatum odoratum Production Area and Its Comparison with Traditional Processing

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Medicinal Plant 2024年第1期15卷 38-40,44页

作者： Miao YANG Chufan PENG Haixia LU Rufeng LIANG Hui tang Zhongmei qin Shengjiu GU Guilin Medical University Guilin 541000China Guangxi Institute of Botany Chinese Academy of SciencesGuilin 541006China Blood Purification Center of Guilin People's Hospital Guilin 541002China

[Objectives]To compare the effects of traditional processing and fresh processing on the quality of Polygonatum odoratum decoction piece.[Methods]The effects of fresh processing and traditional processing on the quality of *** decoction piece were compared and analyzed with appearance characteristics,total ash content,extract content,total polysaccharides content,and total flavonoids content as the evaluation indexes.[Results]Fresh processing method in different production areas has different effects on *** decoction *** was dried in oven of 50℃.When moisture content was 41.44%-59.67%,it was *** complete drying at 50℃,the moisture content of dried *** was 8.94%-9.60%,and ethanol-soluble extract content was 77.29%-78.20%,and water-soluble extract was 77.7%-78.14%.At this time,the appearance characteristics of section of *** decoction piece were better than that of traditional processing,which was yellowish *** total polysaccharide content was higher than that of traditional processing,and the content of total flavonoids was statistically significant different from that of traditional processing.[Conclusions]The quality of *** decoction piece by fresh processing is better than that of the traditional processing,and it is feasible to use fresh processing.

关键词： Processing technique Plygonatum odoratum Fresh processing Total polysaccharide Total flavonoids

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大直径单桩基础稳桩平台结构强度分析

船海工程 2024年第3期53卷 107-112,119页

作者：张犇覃廖开江苏龙源振华海洋工程有限公司海上风电工程技术研究院江苏南通226014

针对自升式海上风电安装船/平台自带的抱桩器纠偏载荷较小、传统稳桩平台作业水深有限、均无法满足大直径单桩基础沉桩作业需求等问题,设计一款可适用于大直径单桩基础施工的新型稳桩平台。考虑稳桩平台底部有支撑和无支撑两种情况,采用... 详细信息

针对自升式海上风电安装船/平台自带的抱桩器纠偏载荷较小、传统稳桩平台作业水深有限、均无法满足大直径单桩基础沉桩作业需求等问题,设计一款可适用于大直径单桩基础施工的新型稳桩平台。考虑稳桩平台底部有支撑和无支撑两种情况,采用SESAM软件对其整体结构强度进行计算分析;基于DNV规范,分别采用手工计算校核和ANSYS Workbench软件模拟两种方式,对其主吊耳局部结构强度进行评估。结果表明,新型稳桩平台整体结构强度和主吊耳局部结构强度均满足要求,主吊耳有限元计算应力值较手工校核应力值更大。

关键词：大直径单桩基础稳桩平台结构强度

Silicone oil migrating into the conjunctival space and orbit after surgery for an eye-penetrating injury:A case report

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World Journal of Clinical Cases 2024年第19期12卷 3950-3955页

作者： ben-Liang Shu Hong-Yun Wu Yu-Xiang Hu Jie Rao Bin Wei qin-Yi Huang Xiao-Rong Wu Department of Ophthalmology The First Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Department of Ophthalmology Ganzhou People's HospitalGanzhou 341000Jiangxi ProvinceChina

BACKGROUND We report a case of eye-penetrating injury in which a massive silicone oil migration into the patient’s subconjunctival space and orbit occurred after *** SUMMARY A 30-year-old male patient sought medical attention at Ganzhou People’s Hospital after experiencing pain and vision loss in his left eye due to a nail wound on December 9,*** of penetrating injury caused by magnetic foreign body retention in the left eye and hospitalization for *** December 9,2023,pars plana vitrectomy was performed on the left eye for intraocular foreign body removal,abnormal crystal extraction,retinal *** to the discovery of retinal detachment at the posterior pole during surgery,silicone oil was injected to fill the vitreous body,following which upper conjunctival bubble-like swelling was *** orbital computed tomography(CT)review indicated migration of silicone oil to the subconjunctival space and orbit through a self-permeable *** December 18,2023,the patient sought treatment at the First Affiliated Hospital of Nanchang University,*** patient presented with a pronounced foreign body sensation following left eye *** December 20,2023,the foreign body was removed from the left eye frame and an intraocular examination was *** posterior scleral tear had closed,leading to termination of the surgical procedure following supplementary laser treatment around the *** patient reported a significant reduction in ocular surface symptoms just one day after ***,a notable decrease in the migration of silicone oil was observed in orbital CT *** The timing of silicone oil injection for an eye-penetrating injury should be carefully evaluated to avoid the possibility of silicone oil migration.

关键词： Silicone oils Migration Vitrectomy Eye injuries Penetrating Case report

Associations of sleeping patterns and isotemporal substitution of other behavior with the prevalence of CKD in Chinese adults

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Frontiers of Medicine 2024年第2期18卷 303-314页

作者： Yi Ding Xiaoli Xu Zhuojun Xin Qiuyu Cao Jiaojiao Huang Xianglin Wu Yanan Huo qin Wan Yingfen qin Ruying Hu Lixin Shi qing Su Xuefeng Yu Li Yan Guijun qin Xulei tang Gang Chen Min Xu Tiange Wang Zhiyun Zhao Zhengnan Gao Guixia Wang Feixia Shen Zuojie Luo Li Chen Qiang Li Zhen Ye Yinfei Zhang Chao Liu Youmin Wang Tao Yang Huacong Deng Lulu Chen Tianshu Zeng Jiajun Zhao Yiming Mu Shengli Wu Yuhong Chen Jieli Lu Weiqing Wang Guang Ning Yu Xu Yufang Bi Mian Li Department of Endocrine and Metabolic Diseases Shanghai Institute of Endocrine and Metabolic DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai200025China Shanghai National Clinical Research Center for Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of ChinaShanghai Key Laboratory for Endocrine TumorState Key Laboratory of Medical GenomicsRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai200025China Jiangxi Provincial People’s Hospital Affiliated to Nanchang UniversityNanchang330000China Department of Endocrinology The Affiliated Hospital of Southwest Medical UniversityLuzhou646000China Department of Endocrinology The First Affiliated Hospital of Guangxi Medical UniversityNanning530000China Zhejiang Provincial Center for Disease Control and Prevention Hangzhou310000China Department of Endocrinology Affiliated Hospital of Guiyang Medical UniversityGuiyang550000China Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200000China Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430000China Sun Yat-sen Memorial Hospital Sun Yat-sen UniversityGuangzhou510000China Department of Endocrinology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou450000China Department of Endocrinology The First Hospital of Lanzhou UniversityLanzhou730000China Fujian Provincial Hospital Fujian Medical UniversityFuzhou350000China Dalian Municipal Central Hospital Affiliated of Dalian Medical UniversityDalian116000China Department of Endocrinology The First Hospital of Jilin UniversityChangchun130000China Department of Endocrinology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou325000China Qilu Hospital of Shandong University Jinan250000China Department of Endocrinology The Second Affiliated Hospital of Harbin Medical UniversityHarbin150000China Department of Endocrinology Cen

Studies have found a U-shaped relationship between sleep duration and chronic kidney disease(CKD)risk,but limited research evaluated the association of reallocating excessive sleep to other behavior with *** included 104538 participants from the nationwide cohort of the Risk Evaluation of Cancers in Chinese Diabetic Individuals:A Longitudinal Study,with self-reported time of daily-life *** isotemporal substitution models,we found that substituting 1 h of sleeping with sitting,walking,or moderate-to-vigorous physical activity was associated with a lower CKD ***-time physical activity displacement was associated with a greater prevalence reduction than occupational physical activity in working *** stratified analysis,a lower CKD prevalence related to substitution toward physical activity was found in long *** pronounced correlations were observed in long sleepers with diabetes than in those with prediabetes,and they benefited from other behavior substitutions toward a more active *** U-shaped association between sleep duration and CKD prevalence implied the potential effects of insufficient and excessive sleep on the kidneys,in which the pernicious link with oversleep could be reversed by time reallocation to physical *** divergence in the predicted effect on CKD following time reallocation to behavior of different domains and intensities and in subpopulations with diverse metabolic statuses underlined the importance of optimizing sleeping patterns and adjusting integral behavioral composition.

关键词： sleep physical activity chronic kidney disease isotemporal substitution behavioral pattern

含空间自相关误差的空间自回归模型的调整经验似然推断

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广西师范大学学报（自然科学版） 2024年第4期42卷 100-114页

作者：唐洁秦永松广西师范大学数学与统计学院广西桂林541006 广西多源信息挖掘与安全重点实验室(广西师范大学) 广西桂林541004

本文研究含空间自相关误差的空间自回归模型的调整经验似然推断问题。利用调整经验似然方法,构造出含空间自相关误差的空间自回归模型的调整经验似然比统计量,证明调整经验似然统计量的极限分布为卡方分布,并模拟比较调整经验似然与一... 详细信息

本文研究含空间自相关误差的空间自回归模型的调整经验似然推断问题。利用调整经验似然方法,构造出含空间自相关误差的空间自回归模型的调整经验似然比统计量,证明调整经验似然统计量的极限分布为卡方分布,并模拟比较调整经验似然与一般经验似然方法的优劣。模拟结果表明:调整经验似然比一般经验似然置信域的覆盖精度更高,计算速度更快。在样本容量较大时,无论误差项是否服从正态分布,一般经验似然方法和调整经验似然方法均可;在样本容量较小时,推荐使用调整经验似然方法。

关键词：空间自相关误差空间SARAR模型调整经验似然覆盖率

白铅矿(PbCO_(3))和菱锌矿(ZnCO_(3))硫化机理的DFT研究

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Transactions of Nonferrous Metals Society of China 2024年第7期34卷 2328-2341页

作者：陈晔唐箫琴陈建华刘萌广西大学资源环境与材料学院广西高校矿物工程重点实验室省部共建特色金属与组合结构全寿命安全国家重点实验室南宁530004 云南省战略金属矿产资源绿色分离与富集重点实验室昆明650093 广西大学化学化工学院南宁530004 四川大学化工学院低碳技术与化学反应工程研究室成都610065

采用密度泛函理论(DFT)比较研究白铅矿(PbCO_(3))和菱锌矿(ZnCO_(3))硫化机理的差异。通过在PbCO_(3)/ZnCO_(3)表面构建Pb-S/Zn-S层来模拟硫化结构。层距和Mulliken电荷的结果表明,PbCO_(3)上形成稳定的Pb-S层,而ZnCO_(3)上形成的Zn-S层不稳定。由于Pb-S层具有高的共价性,而Zn-S层具有高的离子性,因此,硫化-黄药法对PbCO_(3)浮选有效,对ZnCO_(3)浮选无效。为了回收ZnCO_(3),需要强离子性捕收剂,因此,硫化-胺法在ZnCO_(3)浮选中应用较广。PbCO_(3)和ZnCO_(3)表面硫化层结构和浮选行为差异主要是由于金属离子(Pb^(2+)和Zn^(2+))和配体(O配体和S配体)极化率的不同以及Zn^(2+)3d^(10)轨道的惰性造成的。

关键词：密度泛函理论白铅矿菱锌矿硫化-黄药法硫化-胺法

Chemical Composition, Pharmacological Effects and Clinical Applications of Cinobufacini

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Chinese Journal of Integrative Medicine 2024年第4期30卷 366-378页

作者： ZUO Qian XU Ding-qiao YUE Shi-jun FU Rui-jia tang Yu-ping Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility Shaanxi University of Chinese MedicineXi'an(712046)China

Chinese medicine cinobufacini is an extract from the dried skin of Bufo bufo gargarizans Cantor,with active ingredients of bufadienolides and indole *** further research and clinical applications,it is found that cinobufacini alone or in combination with other therapeutic methods can play an anti-tumor role by controlling proliferation of tumor cells,promoting apoptosis,inhibiting formation of tumor neovascularization,reversing multidrug resistance,and regulating immune response;it also has the functions of relieving cancer pain and regulating immune *** this paper,the chemical composition,pharmacological effects,clinical applications,and adverse reactions of cinobufacini are ***,the extraction of monomer components of cinobufacini,the relationship between different mechanisms,and the causes of adverse reactions need to be further ***,high-quality clinical studies should be conducted.

关键词： cinobufacini chemical composition Chinese medicine pharmacological effect clinical application adverse effect

Bright, photostable and long-circulating NIR-Ⅱ nanoparticles for whole-process monitoring and evaluation of renal transplantation

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National Science Review 2024年第2期11卷 207-220页

作者： Rongyuan Zhang Ping Shen Yu Xiong Tianjing Wu Gang Wang Yucheng Wang Liping Zhang Han Yang Wei He Jian Du Xuedong Wei Siwei Zhang Zijie Qiu Weijie Zhang Zheng Zhao ben Zhong tang Clinical Translational Research Center of Aggregation-Induced Emission The Second Affiliated HospitalSchool of MedicineSchool of Science and Engineering Shenzhen Institute of Aggregate Science and TechnologyThe Chinese University of Hong KongShenzhen(CUHK-Shenzhen) Center for AIE Research Shenzhen Key Laboratory of Polymer Science and TechnologyGuangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and Engineering Shenzhen University School of Chemistry Xiangtan University HKUST-Shenzhen Research Institute Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction The Hong Kong University of Science and Technology Department of Urology The First Affiliated Hospital of Soochow University AIE Institute

Kidney transplantation is the gold standard for the treatment of end-stage renal diseases(ESRDs). However,the scarcity of donor kidneys has caused more and more ESRD patients to be stuck on the waiting list for transplant surgery. Improving the survival rate for renal grafts is an alternative solution to the shortage of donor kidneys. Therefore, real-time monitoring of the surgical process is crucial to the success of kidney transplantation, but efficient methods and techniques are lacking. Herein, a fluorescence technology based on bright, photostable and long-circulating aggregation-induced emission(AIE) active NIR-II nano-contrast agent DIPT-ICF nanoparticles for the whole-process monitoring and evaluation of renal transplantation has been reported. In the aggregated state, DIPT-ICF exhibits superior photophysical properties compared with the commercial dyes IR-26 and IR-1061. Besides, the long-circulating characteristic of the AIE nano-contrast agent helps to achieve renal angiography in kidney retrieval surgery,donor kidney quality evaluation, diagnosing vascular and ureteral complications, and assessment of renal graft reperfusion beyond renovascular reconstruction, which considerably outperforms the clinically approved indocyanine green(ICG).

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