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Development and characterization of a novel common wheat–Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance

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Journal of Integrative Agriculture 2023年第5期22卷 1291-1307页

作者： LI Jiao-jiao ZHAO Li Lü Bo-ya FU Yu ZHANG Shu-fa LIU Shu-hui YANG qun-hui WU Jun LI Jia-chuang CHEN Xin-hong Shaanxi Key Laboratory of Plant Genetic Engineering Breeding College of AgronomyNorthwest A&F UniversityYangling 712100P.R.China

Rye(Secale cereale L., 2n=2x=14, RR) is a significant genetic resource for improving common wheat because of its resistance to multiple diseases and abiotic-stress tolerant traits. The 1RS chromosome from the German cultivated rye variety Petkus is critical in wheat breeding. However, its weakened disease resistance highlights the need to identify new resources. In the present study, a novel derived line called D27 was developed from common wheat and Mexico *** observations characterized the karyotype of D27 as 2n=42=21 Ⅱ. Genomic in situ hybridization indicated that a pair of whole-arm translocated Mexico Rye chromosomes were inherited typically in the mitotic and meiosis stages of D27. Experiments using fluorescence in situ hybridization(FISH) and gliadin electrophoresis showed that D27 lacked wheat 1DS chromosomes. They were replaced by 1RS chromosomes of Mexico Rye, supported by wheat simple-sequence repeat markers, rye sequence characterized amplified region markers, and wheat 40K SNP array *** wheat 1DS chromosomes could not be detected by molecular markers and wheat SNP array, but the presence of rye 1RS chromosomes was confirmed. Agronomic trait assessments indicated that D27 had a higher tiller number and enhanced stripe rust and powdery mildew resistance. In addition, dough properties analysis showed that replacing 1DS led to higher viscosity and lower dough elasticity in D27, which was beneficial for cake making. In conclusion, the novel cytogenetically stable common wheat–Mexico Rye T1DL·1RS translocation line D27 offers excellent potential as outstanding germplasm in wheat breeding programs focusing on disease resistance and yield improvement. Additionally,it can be valuable for researching the rye 1RS chromosome’s genetic diversity.

关键词： common wheat disease resistance dough properties rye translocation line

Diagnosis and surgical treatment of post-infarction left ventricular pseudoaneurysm

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Chinese Medical Journal 2009年第2期122卷 232-235页

作者： GAN hui-li ZHANG Jian-qun Cardiac Surgery Department Beijing Anzhen Hospital Capital Medical University Beijing Institute of Heart Lung and Blood Vessel Disease Beijing 100029 China

Left ventricular pseudoaneurysm （LVPA） is a rare clinical entity that could be life-threatening. It could be of congenital and acquired origin. Among the acquired LVPA, post-infarction LVPA constitutes most of the cases. LVPA always occurs after acute myocardial infarction （MI） and develops chronically. Reliable diagnosis and early surgical intervention are vital for the patients＇ recovery. Here we present 7 cases of LVPA in Anzhen Hospital between May 1993 to March 2007, focusing on the evaluation of diagnosis methods and treatment efficiency of LVPA.

关键词： pseudoaneurysm therapeutics diagnosis

ABORTED GAMETOPHYTE 1 is required for gametogenesis in Arabidopsis

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Journal of Integrative Plant Biology 2015年第12期57卷 1003-1016页

作者： Hong-hui Cui Hong-Ze Liao Yu tang Xin-Yu Du Li-qun Chen De Ye Xue-Qin Zhang State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University

In flowering plants, the male and female gameto- genesis is a crucial step of sexual reproduction. Although many genes have been identified as being involved in the gametogenesis process, the genetic mechanisms underlying gametogenesis remains poorly understood. We reported here characterization of the gene, ABORTED GAMETOPHYTE 1 （AOGI） that is newly identified as essential for gametogenesis in Arabidopsis thaliana. AOG1 is expressed predominantly in reproductive tissues including the developing pollen grains and ovules. The AOG1 protein shares no significant amino acid sequence similarity with other documented proteins and is located mainly in nuclei of the cells. Mutation in AOG~ caused degeneration of pollen at the uninucleate microspore stage and severe defect in embryo sacs, leading to a significant reduction in male and female ***, the molecular analyses showed that the aogl mutant significantly affected the expression of several genes, which are required for gametogenesis. Our results suggest that AOG1 plays important roles in gameto- genesis at the stage prior to pollen mitosis 1 （PMI） in Arabidopsis, possibly through collaboration with other genes.

关键词： Arabidopsis embryo sacs gametogenesis pollen grains

A special type of endovascular stent repair with complicated thoracic aneurysm and chronic type B dissection aligned in tandem： double perfusion in true and false distal aorta lumen

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Chinese Medical Journal 2008年第15期121卷 1484-1486页

作者： GAN hui-li ZHANG Jian-qun Cardiac Surgery Department Beijing Anzhen Hospital Capital Medical University Beijing Institute of Heart Lung and Blood Vessel Disease Beijing 100029 China

Aortic dissection and aortic aneurysm are two of the most common catastrophic events involving the aorta. Thoracic endovascular aortic repair is now considered as a promising alternative to open surgical graft replacement. The aim of endovascular repair of a thoracic aneurysm is to exclude, and thus depressurize, the aneurismal wall and the aim of the endovascular repair of type B aortic dissection is to obliterate all of the false lumen through thrombosis after sealing the primary entry tears, thus to ensure the true lumen perfusion. But in some special pathologies, such as when the aneurysm and chronic type B dissection are aligned in tandem, or when a visceral branch originates from the false lumen, how should the endovascular repair strategy proceed in this situation？ For the endovascular stent repair of some special chronic type B aortic dissection, the false lumen cannot be obliterated, and the true and false lumens in the dissected but with a normal diameter distal aorta need to be perfused at the same time, as practiced in the surgery treatment. In this report, we present a case of endovascular stent repair for a special thoracic aneurysm and chronic type B aortic dissection aligned in tandem.

关键词： aortic aneurysm aortic dissection stent

In vivo antimalarial activity of synthetic hepcidin against Plasmodium berghei in mice

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Chinese Journal of Natural Medicines 2017年第3期15卷 161-167页

作者： FANG Ya-qun SHEN Chuan-Bin LUAN Ning YAO hui-Min LONG Chen-Bo LAI Ren YAN Xiu-Wen College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China Key Laboratory of Animal Models and Human Disease Mechanisms Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China University of Chinese Academy of Sciences Beijing 100009China

The present study was designed to investigate the antimalarial activity of synthetic hepcidin and its effect on cytokine secretion in mice infected with Plasmodium *** mice were infected with *** intravenously and treated with hepcidin according to 4-day suppression test and Rane's *** serum levels of interleukins(IL-1β,IL-2,IL-6,IL-10,IL-12p70,and IL-17A),tumor necrosis factor-α(TNF-α),and interferon-γ(IFN-γ) in the experimental mice were determined using a cytometric bead array(CBA)*** survival rate of the infected mice was also ***,the serum iron,alanine transaminase(ALT),aspartate transaminase(AST),and total bilirubin(BIL) were detected to evaluate liver *** exerted direct anti-malarial function in vivo and increased survival rate in a dose-dependent *** addition,the secretion of T helper cell type 1(Th1),Th2,and Th17 cytokines,TNF-α,and IFN-γ were inhibited by *** conclusion,our results demonstrated that synthetic hepcidin exerts in vivo antimalarial activity and possesses anti-inflammatory function,which provides a basis for future design of new derivatives with ideal anti-malarial activity.

关键词： Hepcidin Malaria Cytokine Inflammation Iron

Simultaneous monitoring of the fluorescence and refractive index by surface plasmon coupled emission: A proof-of-concept study

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Chinese Chemical Letters 2023年第10期34卷 82-85页

作者： Lin-Tao Xu Kai-Xin Xie Shuo-hui Cao Yu-Hua Weng Min Chen Zhao Li Yao-qun Li Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis&Instrumentation College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Department of Chemistry Taiyuan Normal UniversityJinzhong 030619China Department of Electronic Science Xiamen UniversityXiamen 361005China

Simultaneous acquisition of fluorescence property and refractive index using a single surface plasmon coupled emission(SPCE)measurement has been achieved,thus achieving synchronicity in real *** SPCE sensor was employed for monitoring the adsorption of volatile organic compounds(VOCs)by dyeencapsulated metal-organic frameworks(Dye@MOFs).Refractive index can reveal surface molecular adsorption and the fluorescence with information on refractive index can provide a comprehensive analysis of the adsorption events of VOCs on the ***,the signal intensity can be amplified by combining the responses caused by changes in refractive index and the fluorescence property in *** all-in-one method opens up a route to monitoring multiple processes simultaneously occurring on the interface.

关键词： Fluorescence with refractive index information Surface plasmon coupled emission Volatile organic compounds Dye-encapsulated metal-organic frameworks All-in-one method

Neospora caninum immune mapped protein 1(NcIMP1) is a novel vaccine candidate against neosporosis

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Frontiers of Agricultural Science and Engineering 2015年第1期2卷 66-72页

作者： Xia CUI Daoyu YANG Tao LEI hui WANG Pan HAO qun LIU Key Laboratory of Zoonosis of Ministry of Agriculture and National Animal Protozoa Laboratory College of Veterinary Medicine China Agricultural University

The Neospora caninum immune mapped protein 1(Nc IMP1) was identified as a membrane protein,and a previous study indicated that Nc IMP1 could be a promising vaccine candidate against neosporosis. In this study, the immune response and protection efficacy of Nc IMP1 were evaluated. The coding sequence of Nc IMP1 was inserted into the eukaryotic expression vector pc DNA3.1(+), resulting in the recombination plasmid pc DNAIMP1, which was used for the intramuscular immunization of BALB/c mice. After immunization, the immune response was evaluated using a lymphoproliferative assay and cytokine and antibody measurements. Quantification of the cerebral parasite burden of mice challenged with 2106 N. caninum was performed 14 days after the last immunization. The results showed that the mice immunized with pc DNA-IMP1 developed a high level of specific antibody responses against recombinant Nc IMP1,with a mixed Ig G1/Ig G2 a response and a predominance of Ig G2 a production. The cellular immune response was associated with the production of IFN-γ, IL-2, IL-4 and IL-10 cytokines. The experiment was terminated 30 days p.i.,and the cerebral parasite burden in each mouse was assessed by quantitative PCR. The parasite burden was significantly reduced in the pc DNA-IMP1-vaccinated mice. These data suggest that IMP1 is a promising vaccine candidate against neosporosis.

关键词： Neospora caninum immune mapped protein 1(IMP1) vaccine candidate BALB/c mice

The Molecular Recognition Characteristics of ω-MercaptoMethoxy Poly(Ethylene Glycol) Self-assembled Monolayer at Gold Electrode and the Detection of Dopamine in Serum

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Chinese Chemical Letters 1998年第2期9卷 165-168页

作者： Shi Feng HOU Ke Shen YANG hui qun FANG and Hong Yuan CHEN(Department of Chemistry, Naning University, Nanjing 210093) Department of Chemistry Nanjing University Nanjing 210093 China

Self assembled monolayers (SAMs) of to-mercapto methoxy poly(ethylene glycol)(MPEG) on gold electrode were used as a means to detect dopamine. Dopamine (DA) had good response at the MPEG film electrode and ascorbic acid (AA) was repelled from the SAMs. TheMPEG film is a biocompatible film, there was no adsorption of biosample and no inactivation atelectrode surface when it was used to detect DA in biosamples.

关键词： Self-assembled Monolayers Electrode Detection Dopamine

Global research trends and prospects of cellular metabolism in colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 527-542页

作者： Yan-Chen Liu Zhi-Cheng Gong Chao-qun Li Peng Teng Yan-Yan Chen Zhao-hui Huang Wuxi Cancer Institute Affiliated Hospital of Jiangnan UniversityWuxi 214122Jiangsu ProvinceChina Wuxi School of Medicine Jiangnan UniversityWuxi 214122Jiangsu ProvinceChina Wuxi Institute of Integrated Chinese and Western Medicine Affiliated Hospital of Jiangnan UniversityWuxi 214062Jiangsu ProvinceChina

BACKGROUND An increasing number of studies have focused on the role of cellular metabolism in the development of colorectal cancer(CRC).However,no work is currently available to synthesize the field through *** To analyze the development in the field of“glucose metabolism”(GM),“amino acid metabolism”(AM),“lipid metabolism”(LM),and“nucleotide metabolism”(NM)in CRC by *** Articles within the abovementioned areas of GM,AM,LM and NM in CRC,which were published from January 1,1991,to December 31,2022,are retrieved from the Web of Science Core Collection and analyzed by CiteSpace 6.2.R4 and VOSviewer *** The field of LM in CRC presented the largest number of annual publications and the fastest increase in the last decade compared with the other three ***,China and the United States were two of the most prominent contri-butors in these four *** addition,Gang Wang,Wei Jia,Maria Notar-nicola,and Cornelia Ulrich ranked first in publication numbers,while Jing-Yuan Fang,Senji Hirasawa,Wei Jia,and Charles Fuchs were the most cited authors on average in these four fields,respectively.“Gut microbiota”and“epithelial-mesenchymal transition”emerged as the newest burst words in GM,“gut microbiota”was the latest outburst word in AM,“metastasis”,“tumor microenvironment”,“fatty acid metabolism”,and“metabolic reprogramming”were the up-to-date outbreaking words in LM,while“epithelial-mesenchymal transition”and“apoptosis”were the most recently occurring words in *** Research in“cellular metabolism in CRC”is all the rage at the moment,and researchers are particularly interested in exploring the mechanism to explain the metabolic alterations in *** metabolic vulnerability appears to be a promising direction in CRC therapy.

关键词： Cellular metabolism Colorectal cancer Glucose metabolism Amino acid metabolism Lipid metabolism Nucleotide metabolism