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An ubiquitin-like protein SDE2 negatively affects sucrose-induced anthocyanin biosynthesis in Arabidopsis

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Science Bulletin 2017年第23期62卷 1585-1592页

作者： Ye Xie Yulei Zhao huijuan tan Qin Chen Jirong Huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China University of the Chinese Academy of Sciences Beijing 100049 China School of Life Sciences Shanghai University Shanghai 200444 China College of Life and Environmental Sciences Shanghai Normal University Shanghai 200234 China

Anthocyanin biosynthesis is regulated by a conserved transcriptional MBW complex composed of MYB,b HLH and WD40 subunits. However, molecular mechanisms underlying transcriptional regulation of these MBW subunits remain largely elusive. In this study, we isolated an Arabidopsis mutant that displays a constitutive red color in aboveground tissues with retarded growth phenotypes. In the presence of sucrose, the mutant accumulates more than 3-fold anthocyanins of the wild type(WT), but cannot produce anthocyanins as WT in the absence of sucrose. Map-based cloning results demonstrated that the mutation occurs in the locus At4 G01000, which encodes a conserved nuclear-localized ubiquitin-like(UBL) superfamily protein, silencing defective 2(SDE2), in eukaryotes. SDE2 is ubiquitously expressed in various tissues. In the sucrose-induced anthocyanin biosynthesis, SDE2 expression was not responded to sucrose treatment at the early stage but was enhanced at the late stage. SDE2 mutations result in upregulation of anthocyanin biosynthetic and regulatory genes. Yeast-two hybrid analysis indicated that SDE2 has no direct interaction with the MYB transcription factor PAP1 and b HLH factor TT8, indicating that SDE2 is a indirect factor to affect anthocyanin accumulation. Taking together, our data suggest that SDE2 may play a role in finely coordinating anthocyanin biosynthesis with other biological processes.

关键词： Anthocyanin biosynthesis Sucrose SDE2 PAP1 Arabidopsis

DELLA Proteins Promote Anthocyanin Biosynthesis via Sequestering MYBL2 and JAZ Suppressors of the MYB/bHLH/WD40 Complex in Arabidopsis thaliana

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Molecular Plant 2016年第5期9卷 711-721页

作者： Ye Xie huijuan tan Zhaoxue Ma Jirong Huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and EcologyShanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China University of the Chinese Academy of Sciences Beijing 100049 China

Anthocyanin accumulation is recognized as a visible biomarker of plants that have suffered from environmental stresses. However, the molecular mechanisms underlying stress-induced anthocyanin biosynthesis remain unclear. Expression of anthocyanin-specific genes is regulated by the conserved MBW complex, which is composed of the MYB, bHLH, and WD40 subunRs in higher plants. MBW activity is repressed by MYBL2 and the JAZ family proteins, which bind competitively to bHLH and MYB/bHLH, respectively. Here, we found that MYBL2 and JAZs mediate gibberellic acid-inhibRed anthocyanin biosynthesis in Arabidopsis. Competitive pull-down and dual-lucifarase assays showed that DELLA proteins directly sequester MYBL2 and JAZ repressors, leading to the release of bHLH/MYB subunits and subsequently to the formation of active MBW complex, which then activates the anthocyanin biosynthetic pathway. The JAZ-DELLA-MYBL2 module also plays an Important role in abiotic stress-induced anthocy- anin biosynthesis. Furthermore, we found that the DELLA protein RGA accumulates upon plant exposure to abiotic stresses. Altogether, our data reveal that DELLA-promoted anthocyanin biosynthesis is mediated at least in part by MYBL2 and JAZ regulatory proteins, providing new insights into the coordinated regulation of plant growth and defense through metabolic pathway regulation.

关键词： DELLA MYBL2 JAZ anthocyanin biosynthesis abiotic stresses

Patterns of shrub species richness and abundance in relation to environmental factors on the Alxa Plateau:Prerequisites for conserving shrub diversity in extreme arid desert regions

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Science China Earth Sciences 2009年第5期52卷 669-680页

作者： XinRong Li huijuan tan MingZhu He XinPing Wang XiaoJun Li Shapotou Desert Research and Experiment Station Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 China

Shrub species are considered the dominant plants in arid desert ecosystems, unlike in semiarid steppe zones or in grassland ecosystems. On the Alxa Plateau, northern China, sparse vegetation with cover ranging from 15... 详细信息

关键词： biodiversity conservation shrub species richness abundance Alxa Plateau desert ecosystems

A Crucial Role of GA-Regulated Flavonol Biosynthesis in Root Growth of Arabidopsis

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Molecular Plant 2019年第4期12卷 521-537页

作者： huijuan tan Cong Man Ye Xie Jijun Yan Jinfang Chu Jirong Huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China University of the Chinese Academy of Sciences Beijing 100049 China National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Shanghai Key Laboratory of Plant Molecular Sciences College of Life Sciences Shanghai Normal University Shanghai 200234 China

Flavonols have been demonstrated to play many important roles in plant growth, development, and communication with other organisms. Flavonol biosynthesis is spatiotemporally regulated by the subgroup 7 R2R3-MYB (SG7 MYB) transcription factors including MYB11/MYB12/MYB111. However, whether SG7- MYB activity is subject to post-translational regulation remains unclear. Here, we show that gibberellic acid (GA) inhibits flavonol biosynthesis via DELLA proteins in Arabidopsis. Protein-protein interaction analyses revealed that DELLAs (RGA and GAI) interacted with SG7 MYBs (MYB12 and MYB111) both in vitro and in vivo, leading to enhanced affinity of MYB binding to the promoter regions of key genes for flavonol biosynthesis and thus increasing their transcriptional levels. We observed that the level of auxin in the root tip was negatively correlated with root flavonol content. Furthermore, genetic assays showed that loss-of-function mutations in MYB 12, which is predominantly expressed in roots, partially rescued the short-root phenotype of the GA-deficient mutant ga1-3 by increasing root meristem size and mature cell size. Consistent with these observations, exogenous application of the flavonol quercetin restored the root meristem size of myb12 ga1-3 to that of ga1-3. Taken together, our data elucidate a molecular mechanism by which GA promotes root growth by directly reducing flavonol biosynthesis.

关键词： gibberellin flavonol root growth auxin stress

Arogenate Dehydratase Isoforms Differentially Regulate Anthocyanin Biosynthesis in Arabidopsis thaliana

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Molecular Plant 2016年第12期9卷 1609-1619页

作者： Qingbo Chen Cong Man Danning Li huijuan tan Ye Xie Jirong Huang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China These authors contributed equally to this article

Anthocyanins, a group of L-phenylalanine （Phe）-derived flavonoids, have been demonstrated to play impor- tant roles in plant stress resistance and interactions between plants and insects. Although the anthocyanin biosynthetic pathway and its regulatory mechanisms have been extensively studied, it remains unclear whether the level of Phe supply affects anthocyanin biosynthesis. Here, we investigated the roles of arogenate dehydratases （ADTs）, the key enzymes that catalyze the conversion of arogenate into Phe, in sucrose-induced anthocyanin biosynthesis in Arabidopsis. Genetic analysis showed that all six ADT isoforms function redundantly in anthocyanin biosynthesis but have differential contributions. ADT2 contributes the most to anthocyanin accumulation, followed by ADT1 and ADT3, and ADT4-ADT6. We found that anthocyanin content is positively correlated with the levels of Phe and sucrose-induced ADT transcripts in seedlings. Consistently, addition of Phe to the medium could dramatically increase anthocyanin content in the wild-type plants and rescue the phenotype of the adtl adt3 double mutant regarding the anthocyanin accumulation. Moreover, transgenic plants overexpressing ADT4, which appears to be less sensitive to Phe than overexpression ofADT2, hyperaccumulate Phe and produce elevated level of antho- cyanins. Taken together, our results suggest that the level of Phe is an important regulatory factor for sus- taining anthocyanin biosynthesis.

关键词： phenylalanine metabolic flux ADT anthocyanin Arabidopsis

Ecological restoration and recovery in the wind-blown sand hazard areas of northern China: relationship between soil water and carrying capacity for vegetation in the Tengger Desert

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Science China(Life Sciences) 2014年第5期57卷 539-548页

作者： LI XinRong ZHANG ZhiShan tan huijuan GAO YanHong LIU LiChao WANG XinPing Shapotou Desert Research and Experiment Station Cold and Arid Regions Environmental and Engineering Research InstituteChinese Academy of Sciences Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province

The main prevention and control area for wind-blown sand hazards in northern China is about 320000 km2 in size and includes sandlands to the east of the Helan Mountain and sandy deserts and desert-steppe transitional regions to the west of the Helan *** recovery and restoration is an important and effective approach for constraining wind-blown sand hazards in these *** more than 50 years of long-term ecological studies in the Shapotou region of the Tengger Desert,we found that revegetation changed the hydrological processes of the original sand dune system through the utilization and space-time redistribution of soil *** spatiotemporal dynamics of soil water was significantly related to the dynamics of the replanted vegetation for a given regional precipitation *** long-term changes in hydrological processes in desert areas also drive replanted vegetation *** soil water carrying capacity of vegetation and the model for sand fixation by revegetation in aeolian desert areas where precipitation levels are less than 200 mm are also discussed.

关键词： desert areas sand fixation by plant succession of the artificial vegetation soil water dynamics carrying capacity forvegetation

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现代主义视野下的T.S.艾略特和拉尔夫·埃利森

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外国文学研究 2017年第1期39卷 9-19页

作者：谭惠娟杭州电子科技大学外国语学院

T.S.艾略特是英国现代主义文学运动的一员骁将,其创作主要表现19世纪末到20世纪欧洲的战争危机和现代人的道德瓦解。拉尔夫·埃利森是非裔美国现代主义文学的先锋,其创作旨在争取黑人的身份地位和种族平等,使黑人文学融入白人主流文学... 详细信息

T.S.艾略特是英国现代主义文学运动的一员骁将,其创作主要表现19世纪末到20世纪欧洲的战争危机和现代人的道德瓦解。拉尔夫·埃利森是非裔美国现代主义文学的先锋,其创作旨在争取黑人的身份地位和种族平等,使黑人文学融入白人主流文学。同为现代主义作家,白人作家T.S.艾略特是黑人作家拉尔夫·埃利森的文学前辈,他们之间既有共性,也有差异。他们耳濡目染的是不同的文学传统,但都关注"他者","埃塞俄比亚之风"是触发其现代主义文学创作的艺术灵感之一。他们都具有强烈的使命感,关注和思考人类命运和世界秩序,其现代主义创作动机就是要通过艺术打破既有的人类社会秩序,用音乐语言软化这个充满暴力的混乱世界。他们的文学创作高度艺术化,与历史现实保持一种若即若离的关系,体现出某种超越主流现代主义的独特文风。本文从"埃塞俄比亚之风"、文学艺术、文学传统与历史等方面探讨了艾略特和埃利森的现代主义文学观,旨在证明,他们的现代主义思想丰富了主流现代主义文学,在某种程度上反映了现代主义文学创作的真谛,值得学界重视和世人反思。

关键词： T.S.艾略特拉尔夫·埃利森现代主义埃塞俄比亚之风人类秩序

詹姆斯·鲍德温的文学“弑父”与美国黑人文学的转向

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外国文学研究 2006年第6期28卷 130-138页

作者：谭惠娟浙江大学外语学院

20世纪40年代末,美国黑人作家詹姆斯.鲍德温对他在文学创作上的引路人、当时美国黑人文学的代表人物赖特进行了猛烈攻击,这一举动符合布鲁姆所说的文学发展进程中的“弑父”行为。鲍德温在文学上的“弑父”与他个人生活中的“弑父”情... 详细信息

20世纪40年代末,美国黑人作家詹姆斯.鲍德温对他在文学创作上的引路人、当时美国黑人文学的代表人物赖特进行了猛烈攻击,这一举动符合布鲁姆所说的文学发展进程中的“弑父”行为。鲍德温在文学上的“弑父”与他个人生活中的“弑父”情结相关,而更深层的动因则在于当时美国黑人文学正经历由赖特式的“抗议小说”向拉尔夫.埃利森为代表的新型黑人文学转向。作为其间的过渡人物,鲍德温在描写黑人人性、展示黑人文化、提升黑人文学的艺术水平、探索种族歧视与冲突问题等方面,都提出了超越赖特的抗议文学主张,但他本人的思想观念和创作实践又还存在诸多自相矛盾之处。“弑父”使赖特和鲍德温两败俱伤,具有浓烈悲剧色彩,但它是美国黑人文学发展过程中的一个必然环节,客观上具有积极意义。

关键词：詹姆斯·鲍德温理查德·赖特拉尔夫·埃利森弑父美国黑人文学转向

论拉尔夫·埃利森对神话仪式中黑白二元对立的解构——兼论拉尔夫·埃利森文学话语中的祖先在场

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外国文学研究 2007年第4期29卷 87-96页

作者：谭惠娟浙江大学外语学院

美国黑人作家拉尔夫·埃利森《无形人》的中心隐喻"无形性",是作者对美国黑人文化及其价值观长期思考的结果,这一隐喻标志着美国黑人文学创作由自然主义抗议小说,朝注重艺术美学的现代小说转向。这一转向着重表现在作者对欧洲黑白二元... 详细信息

美国黑人作家拉尔夫·埃利森《无形人》的中心隐喻"无形性",是作者对美国黑人文化及其价值观长期思考的结果,这一隐喻标志着美国黑人文学创作由自然主义抗议小说,朝注重艺术美学的现代小说转向。这一转向着重表现在作者对欧洲黑白二元对立神话和美国南方社会仪式的解构以及作者创作中祖先在场的文学叙述手法的运用。拉尔夫·埃利森对神话和仪式的重新发现和阐释,是作者的文化思想在文学创作中的集中体现之一,也是对20世纪30年代盛行从社会科学的角度研究黑人问题的有力反拨;而祖先在场的文学叙述手法则有助于美国种族主义者重新审视自己的身份和美国文化,有助于破解"无形性"被曲解的含义和真正的内涵。拉尔夫·埃利森对美国文化的认识和解剖,与罗兰·巴特的文化思想和德里达的解构思想相似,这更体现了埃利森文化研究思想和文学叙述手法的独创性与超前性。

关键词：拉尔夫·埃利森神话仪式黑白二元对立解构