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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Rui-Cheng HU,Qing OUYANG,Ai-Guo DAI,Shuang-Xiang tan,zhi-qiang XIAO,Cen-E tanG 1 Department of Respiratory Medicine,Hunan Institute of Gerontology,Hunan Province Geriatric Hospital,Changsha 410016, China 2 Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha 410016,China

＜正＞Background and objective:COPD is a public health problem worldwide with complex pathogenesis, and has not been well *** current study aims to acquire new clues for the pathogenesis of COPD by use of proteomics ***:Lung tissue specimens were harvested from never-smokers,non-COPD smokers and COPD smokers,and the proteins differentially expressed between the 3 group subjects were revealed by two dimensional electrophoresis and sequence identified by matrix assisted laser desorption/ionization time of flight mass spectrometry analysis combined with bioinformation *** interested differentially expressed proteins identified by proteomics technology were validated by immunohistochemistory and ***: Forty seven protein spots were revealed to be differentially expressed between groups with intensity variation more than 1.5 times from two dimensional electrophoresis maps,and 24 proteins were identified through mass spectrometry analysis combined with database *** expressed proteins were functionally mainly involved in basic metabolism,oxidoreduction,coagulation/fibrinolysis,protein degradation,signal transduction, inflammation,cell growth/*** the 24 identified proteins,22 proteins were up-regulated in the lung of smokers compared with *** results of proteomics,immunohistochemical staining and Western-blotting accordant demonstrated that the expression of heat shock protein 27 and Cyclophilin A in lung was increased in non-COPD smokers and further up-regulated in COPD smokers when compared with never-smokers. Conclusion:Heat shock protein 27 and Cyclophilin A participate in the pathogenesis of COPD,and their expression in lung is induced by smoking.

关键词： Pulmonary disease chronic obstructive HSP27 heat-shock proteins Cyclophilin A proteomics

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第01期 288-296页

作者： Shuai Liu Wen-qiang Xu Ying-Tong Di Man-Cheng tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu tang Chun-Mao Yuan Jun-Bo Yang zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

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Review:Advances in research of stress-assisted corrosion fatigue problem

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第2期8卷 221-227页

作者： tanG zhi-bo LI qiang Division of Engineering Zhejiang Ocean University Zhoushan 316004 China

Ceramic materials are notable for their rigidity, insulation and resistance to hostile environment, Nevertheless, if a stressed ceramic component is exposed to chemical attack, it may suffer from a form of delayed fracture known as static fatigue. From the point of view of a designer, it is clearly desirable to determine the behavior of sub-critical crack growth; the crack path and crack growth rate, as a function of material properties and loading conditions are of particular interest. This paper presents a review of advances in stress assisted corrosion problem in history and its corresponding numerical approaches in the last decades, and finally, comes uo with consideration and crucial suggestions for future work.

关键词： Stress corrosion Finite element method (FEM) Fracture Static fatigue Meshless local Petrov-Galerkin (MLPG)

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强心方对慢性心力衰竭大鼠心肌线粒体能量代谢与自噬的影响

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广州中医药大学学报 2022年第7期39卷 1622-1629页

作者：罗远林徐燕梅谭志强周学锋刘焕云乐山市人民医院四川乐山614000 乐山职业技术学院医学系四川乐山614000 重庆两江新区第一人民医院心血管内科重庆401121

【目的】探讨强心方(由温阳活血利水方药组成)对慢性心力衰竭大鼠的治疗机制。【方法】从72只大鼠中选取60只大鼠建立慢性心力衰竭模型,感染死亡5只。将55只成功造模的大鼠按体质量分为模型组,卡托普利组,强心方低、中、高剂量组,每组1... 详细信息

【目的】探讨强心方(由温阳活血利水方药组成)对慢性心力衰竭大鼠的治疗机制。【方法】从72只大鼠中选取60只大鼠建立慢性心力衰竭模型,感染死亡5只。将55只成功造模的大鼠按体质量分为模型组,卡托普利组,强心方低、中、高剂量组,每组11只。另取11只大鼠设为假手术组,仅切口穿线不结扎。建模第1天,强心方低、中、高剂量组大鼠分别灌胃3.96、7.92、15.84(生药)g·kg^(-1)·d^(-1)的强心方混悬液,卡托普利组大鼠灌胃10 g/mL的卡托普利。假手术组和模型组分别灌胃等体积生理盐水溶液。每日1次,连续4周。采用酶联免疫吸附法(ELISA)检测血清中单磷酸腺苷(AMP)、二磷酸腺苷(ADP)、三磷酸腺苷(ATP)含量,考马斯亮蓝法检测心肌组织Na+-K+ATP酶、Ca2+-Mg2+ATP酶活性,苏木素-伊红(HE)染色法观察心肌组织病理形态,免疫荧光法检测心肌组织自噬蛋白微管相关蛋白轻链3Ⅱ(LC3-Ⅱ)阳性表达情况,蛋白免疫印迹(Western Blot)法检测心肌组织中p38丝裂原活化蛋白激酶(p38MAPK)、磷酸化p38MAPK(p-p38MAPK)及过氧化物酶体增殖物激活受体(PPAR)-γ蛋白表达。【结果】(1)与假手术组比较,模型组血清ATP含量和心肌组织Na+-K+ATP酶活性、Ca2+-Mg2+ATP酶活性降低,血清ADP、AMP含量升高(P0.05)。【结论】强心方可能通过抑制p38MAPK活化及促进PPAR-γ蛋白表达进而增强心肌线粒体能量代谢,减少心肌细胞自噬,从而起抗慢性心力衰竭的作用。

关键词：强心方温阳活血利水慢性心力衰竭线粒体能量代谢自噬大鼠

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吉林省东部山区耕地土壤酸化特征研究

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人参研究 2020年第5期32卷 50-53页

作者：殷秀岩隋明义谭志强马遥洛俊峰谢忠凯通化禾韵现代农业股份有限公司通化134001 通化市土壤肥料工作站通化134000

为了研究吉林省东部山区耕地土壤酸化特征,选取了水浸pH值5.0以下的耕地土壤,采集了29份土壤样品,同时采集天然林林地土壤样品12份作为对照,通过土壤检测对比发现,耕地土壤水浸pH值平均4.6,对应的盐浸pH值为4.0,分别比对照下降0.8、0.7... 详细信息

为了研究吉林省东部山区耕地土壤酸化特征,选取了水浸pH值5.0以下的耕地土壤,采集了29份土壤样品,同时采集天然林林地土壤样品12份作为对照,通过土壤检测对比发现,耕地土壤水浸pH值平均4.6,对应的盐浸pH值为4.0,分别比对照下降0.8、0.7个单位,选择的耕地土壤样品表现出重度酸化。选取的耕地土壤具有交换性酸大幅增加、交换性钙镁含量与盐基饱和度大幅下降,这是重度酸化耕地土壤所表现的主要化学特征。区域内耕地土壤酸化的本质是土壤盐基离子的流失。水土流失严重、有机质含量下降、大量化肥特别是氮肥的过量施用、钙镁离子被作物带出农田而得不到补充等方面加速了土壤酸化的过程。吉林省东部山区耕地土壤酸化面积在扩大、酸化程度加重,影响了区域内种植业结构的调整,需要通过根茬与秸秆还田、增施有机肥、增施石灰等碱性材料、实施保护性耕作、实施测土平衡施肥等措施不断地改良培肥土壤,以期达到用养结合可持续利用耕地。

关键词：耕地土壤酸化酸化特征酸化影响因素改良培肥措施

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Onboard real time modeling of aircraft engines with Hammerstein-Wiener representation

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航空动力学报 2014年第10期29卷 2499-2506页

作者： WANG Ji-qiang YE zhi-feng HU Zhong-zhi Jiangsu Province Key Laboratory of Aerospace Power System College of Energy and Power EngineeringNanjing University of Aeronautics and Astronautics

An identification-based approach for aircraft engine modeling using the nonlinear HammersteinWiener representation was ***-Wiener modeling for both limited flight envelope and extended flight envelope was *** shows that the resulting model can be valid over 10%variation of rotational speed of the engine,compared with those linear models that are only valid over 3%—5%change of rotational *** is further demonstrated that the proposed method can be utilized over large envelope up to 20% variation of rotational speed of the *** fundamental idea is to use nonlinear models to extend the feasible/valid region rather than those linear *** may consequently simplify the switching logic in the onboard digital control *** is often overlooked in aircraft engine control community,but has been emphasized in the research.

关键词： aircraft engines engine modeling onboard real time modeling system identification Hammerstein-Wiener representation

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An Evaluation of Value-Oriented Review for Software Requirements Specification

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Computer Systems Science & Engineering 2021年第6期37卷 443-461页

作者： qiang zhi Shuji Morisaki Tokyo Institute of Technology Tokyo152-8550Japan Nagoya University Nagoya464-8601Japan

A software requirements specification(SRS)is a detailed description of a software system to be *** paper proposes and evaluates a lightweight review approach called value-oriented review(VOR)to detect defects in *** approach comprises setting core values based on SRS and detecting the defects disturbing the core *** evaluate the effectiveness of the proposed approach,we conducted a controlled experiment to investigate whether reviewers could identify and record the core values based on SRS and find defects disturbing the core *** of the evaluation with 56 software engineers showed that 91%of the reviewers identified appropriate core values and 82%of the reviewers detected defects based on the identified core ***,the average number of defects detected using the proposed approach was slightly smaller than that detected using perspective-based reading(PBR);however,PBR requires defining review scenarios before attempting to detect any *** results also demonstrated that the proposed approach helped reviewers detect the omission defects,which are more difficult to detect from SRS than defects because of ambiguity or incorrect requirements.

关键词： Value-oriented review perspective-based reading scenario-based reading lightweight review software requirements specification

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Empirically Modeling Enterprise Architecture Using ArchiMate

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Computer Systems Science & Engineering 2022年第1期40卷 357-374页

作者： qiang zhi Zhengshu Zhou Graduate School of Engineering Tokyo Institute of TechnologyTokyoCO 1528550Japan Graduate School of Informatics Nagoya UniversityNagoyaAichiCO 4648601Japan Institute of Innovation for Future Society Nagoya UniversityNagoya UniversityNagoyaAichiCO 4648601Japan

Enterprise Architecture(EA)has evolved based on the practice of information systems architecture design and *** is a rigorous description of a structure,and the objectives of EA modeling not only include clarifying corporate strategies,visualizing business processes,and modeling information systems to manage resources but also include improving organizational structures,adjusting information strategies,and creating new business ***,EA models cover a wide scope that includes both IT and business ***,EA modeling is the initial and most important analysis step for researchers,architects,and *** is the dominant modeling language for EA and it has been shown to improve visualization of EA ***,few studies have systematically introduced general modeling methods using ArchiMate to meet a broad range of needs and few studies have empirically evaluated the modeling method using *** paper introduces an EA visualization approach to fill that gap and conducts a case study on a wilderness weather station *** controlled experiments are conducted to verify the effectiveness and feasibility of this modeling method,and the experimental results show that this modeling procedure is not only feasible,but also can effectively transform the system metamodel and restore the EA scene.

关键词： Enterprise architecture system modeling visualization methodology

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Quantitative Proteomic Analysis of Bromotetrandrine and Tetrandrine in K562 Cell Line Using ^(18)O-labeling Method

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Chinese Herbal Medicines 2012年第1期4卷 43-52页

作者： Ying tan[a] zhi-qiang GE[a] Chang-xiao LIU[b] School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China

Objective To compare quantitative proteomic analysis of bromotetrandrine (W198) which was a Class I new antitumor drug in China and tetrandrine (Tet) in K562 cell line using 18O-labeling method. Methods To illustrate its mechanism, a shotgun quantitative proteomic strategy employing 2D LC-MS-MS and trypsin catalyzed 18O-labeling quantification was carried out in this study. Compared to normal chronic leukemia cell line K562 and K562 induced by Tet, the proteomic changes of K562 induced by W198 were investigated. In order to validate the quantitation by the 18O-labeling, the analysis was done on an equivalent sample composed of the same amount of labeled and unlabeled proteins from normally cultured cells to act as a reference to the comparative sample. Results A threshold of ± 2-fold change for deciding whether a protein concentration was changed was settled for the following experiments. Comparing the 105 identified soluble proteins’ expression levels of the apoptosis starting up K562 cells after W198 induction with the normally cultured cells, 16 proteins were found with significantly altered expression levels after W198 treatment. Eight proteins were up-expressed including HMGB2, peroxiredoxin-2, and eIF4A-I, etc. Eight proteins were down-expressed including TCP-1, GRP94, GST-π, and SFGHs, etc. Compared to K562 induced by Tet, eight proteins of K562 were found with significantly altered expression levels after W198 treatment. Five proteins were up-expressed including HSP 90-β and 40S ribosomal protein S15a, etc. Three proteins were down-expressed including phosphoglycerate kinase 1, isoform 5 of interleukin enhancer-binding factor 3, etc. Conclusion The 18O-labeling MS-MS-based method is ideal as a discovery tool, but it is not suitable for validation using a large number of samples. Other more effective methods, such as Western blotting should be used for further validation of candidate cancer proteins discovered from 18O-labeling samples. In total, 105 soluble

关键词： bromotetrandrine K562 18O-labeling quantitative proteomics tetrandrine

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Characteristics of the reduction behavior of zinc ferrite and ammonia leaching after roasting

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International Journal of Minerals,Metallurgy and Materials 2020年第1期27卷 26-36页

作者： Chao Wang Yu-feng Guo Shuai Wang Feng Chen Yu-jia tan Fu-qiang Zheng Ling-zhi Yang School of Minerals Processing and Bioengineering Central South University

A novel method for recovering zinc from zinc ferrite by reduction roasting–ammonia leaching was studied in this paper. The reduction thermodynamic of zinc ferrite by CO was analyzed. The effects of roasting parameters on the phase transformation and conversion rate of zinc ferrite, and the leaching behavior of zinc from the reductive roasted samples by ammonia leaching, were experimentally investigated. The mineralogical phase compositions and chemical compositions of the samples were characterized by X-ray diffraction and chemical titration methods, respectively. The results showed that most of the zinc ferrite was transformed to zinc oxide and magnetite after weak reduction roasting. 86.43% of the zinc ferrite was transformed to zinc oxide under the optimum conditions: CO partial pressure of 25%, roasting temperature of 750°C, and roasting duration of 45 min. Finally, under the optimal leaching conditions, 78.12% of zinc was leached into the solution from the roasted zinc ferrite while all iron-bearing materials were kept in the leaching residue. The leaching conditions are listed as follows: leaching duration of 90 min,ammonia solution with 6 mol/L concentration, leaching temperature of 50°C, solid-to-liquid ratio of 40 g/L, and stirring speed of 200 r/min.

关键词： zinc ferrite reduction roasting ammonia leaching zinc extraction zinc-bearing waste

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