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高效毛细管电泳同时分离多种酚类物质

中国粮油学报 2010年第6期25卷 114-118,122页

作者：周胜男檀华蓉李慧陆宁安徽农业大学茶与食品科技学院合肥230036 安徽农业大学生物技术中心合肥230036 安徽农业大学动物科技学院合肥230036

建立了高效毛细管电泳同时分离测定芦丁、槲皮素、绿原酸、咖啡酸、没食子酸和原儿茶酸6种酚类物质的分析方法。考察了缓冲液种类、离子浓度和pH、分离电压、运行温度等电泳参数,确立了最佳的电泳条件：缓冲液为20 mmol/L磷酸二氢钠-20 ... 详细信息

建立了高效毛细管电泳同时分离测定芦丁、槲皮素、绿原酸、咖啡酸、没食子酸和原儿茶酸6种酚类物质的分析方法。考察了缓冲液种类、离子浓度和pH、分离电压、运行温度等电泳参数,确立了最佳的电泳条件：缓冲液为20 mmol/L磷酸二氢钠-20 mmol/L硼砂,pH7.5,分离电压为20 kV,运行温度为30℃,紫外检测波长214 nm。结果表明,6种物质在12 m in内得到完全分离,且各组分质量浓度与峰面积呈良好的线性关系,R2为0.998 7~0.987 4。方法精密度试验中,迁移时间的RSD为0.280%~0.425%,峰面积的RDS为5.228%~8.506%,回收率为85.11%~101.98%。该法快速、简便、准确,具有较高的灵敏度。

关键词：高效毛细管电泳同时分离酚酸类

圈卷产色链霉菌7100分化相关基因——sawE的结构与功能

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微生物学报 2003年第1期43卷 48-55页

作者：徐健勇刘钢谭华荣中国科学院微生物研究所北京100080

以天蓝色链霉菌的whiB基因为探针 ,从圈卷产色链霉菌 71 0 0的总DNA部分文库中克隆了含有whiB同源序列的 2 8kbDNA片段 ,并对其中的 1 4kb片段进行了序列测定。序列分析表明 ,该片段含有一个完整的开放阅读框—sawE。预测的蛋白质结... 详细信息

以天蓝色链霉菌的whiB基因为探针 ,从圈卷产色链霉菌 71 0 0的总DNA部分文库中克隆了含有whiB同源序列的 2 8kbDNA片段 ,并对其中的 1 4kb片段进行了序列测定。序列分析表明 ,该片段含有一个完整的开放阅读框—sawE。预测的蛋白质结构及同源性分析显示 ,sawE与天蓝色链霉菌孢子形成早期的关键基因whiB高度同源 ,编码产物为一个调控蛋白。sawE的破坏使圈卷产色链霉菌 71 0 0的分化终止在气生菌丝阶段 ,在延长培养时间的情况下仍保持白色的表型 ,菌丝不能分隔 ,不能形成成熟的灰色孢子 ,结果表明sawE基因是一个与圈卷产色链霉菌分化有关的重要基因。

关键词：圈卷产色链霉菌7100 分化相关基因 sawE 结构功能形态分化

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Chinese Journal of Electronics 2024年第3期33卷 683-693页

作者： Jiamei LIU Lin tan Hong XU PLA Strategic Support Force Information Engineering University

Deoxys-BC is the primitive tweakable block cipher of the Deoxys family of authenticated encryption *** on existing related-tweakey boomerang distinguishers,this paper improves the boomerang attacks on 11-round Deoxys-BC-256 and 13-round Deoxys-BC-384 by the optimized key guessing and the precomputation *** transfers a part of subtweakey guess in the key-recovery phase to the precomputation resulting in a significant reduction of the overall time *** 11-round Deoxys-BC-256,we give a related-tweakey boomerang attack with time/data/memory complexities of 2218.6/2125.7/2125.7,and give another attack with the less time complexity of2215.8and memory complexity of 2120 when the adversary has access to the full *** 13-round Deoxys-BC-384,we give a related-tweakey boomerang attack with time/dat a/memory complexities of 2k-96+2157.5/2120.4/*** the key size k=256,it reduces the time complexity by a factor of 231compared with the previous 13-round boomerang *** addition,we present two new related-tweakey boomerang distinguishers on 11-round Deoxys-BC-384 with the same probability as the best previous distinguisher.

关键词： Ciphers Memory management Complexity theory Encryption Time complexity

Joint Optimization of Trajectory and Task Offloading for Cellular-Connected Multi-UAV Mobile Edge Computing

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Chinese Journal of Electronics 2024年第3期33卷 823-832页

作者： Jingming XIA Yufeng LIU Ling tan School of Artificial Intelligence Nanjing University of Information Science and Technology Engineering Research Center of Digital Forensics Ministry of Education Nanjing University of Information Science and Technology

Since the computing capacity and battery energy of unmanned aerial vehicle(UAV) are constrained,UAV as aerial user is hard to handle the high computational complexity and time-sensitive *** paper investigates a cellular-connected multi-UAV network supported by mobile edge *** UAVs carrying tasks fly from a given initial position to a termination position within a specified *** handle the large number of tasks carried by UAVs,we propose a energy cost of all UAVs based problem to determine how many tasks should be offloaded to high-altitude balloons(HABs) for computing,where UAV-HAB association,the trajectory of UAV,and calculation task splitting are jointly ***,the formulated problem has nonconvex ***,an efficient iterative algorithm by applying successive convex approximation and the block coordinate descent methods is put ***,in each iteration,the UAV-HAB association,calculation task splitting,and UAV trajectory are alternately ***,for the nonconvex UAV trajectory optimization problem,an approximate convex optimization problem is *** numerical results indicate that the scheme of this paper proposed is guaranteed to converge and also significantly reduces the entire power consumption of all UAVs compared to the benchmark schemes.

关键词： Energy consumption Multi-access edge computing Costs Power demand Autonomous aerial vehicles Iterative algorithms Convex functions

土壤B[a]P多次叠加污染的生物有效性及对蚯蚓体腔细胞染色体和溶酶体的毒性效应

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应用与环境生物学报 2014年第6期20卷 1020-1026页

作者：张伟郑彬马静静葛高飞檀华蓉郜红建安徽农业大学资源与环境学院合肥230036 安徽农业大学生物技术中心合肥230036

Benzo(a)pyrene (B[a]P)作为典型具有"三致"效应的持久性有机污染物,在土壤中逐步累积,对土壤生态安全造成潜在威胁.通过一次与多次污染的方法,研究B[a]P在土壤中的有效性特征及其对蚯蚓(Eisenia fetida)体腔细胞染色体畸变及溶酶体膜... 详细信息

Benzo(a)pyrene (B[a]P)作为典型具有"三致"效应的持久性有机污染物,在土壤中逐步累积,对土壤生态安全造成潜在威胁.通过一次与多次污染的方法,研究B[a]P在土壤中的有效性特征及其对蚯蚓(Eisenia fetida)体腔细胞染色体畸变及溶酶体膜完整性的毒性效应.结果显示:随着培养时间的延长,土壤中B[a]P含量及蚯蚓体内B[a]P富集量、蚯蚓体腔细胞微核变率均呈现初期(28 d)下降速率较快,尔后(28-56 d)下降速率减小的趋势;而蚯蚓体腔细胞中性红保留时间(NRRT)升高速率呈先快后慢的趋势.土壤中B[a]P的Tenax法提取含量与蚯蚓体内B[a]P富集量呈极显著正相关(R2=8614,P<0.01);蚯蚓体腔细胞NRRT和微核变率分别与土壤中B[a]P的Tenax法提取含量和蚯蚓体内B[a]P富集量呈显著正相关(P<0.05).相同培养时间内,多次叠加污染土壤中B[a]P的Tenax提取含量和蚯蚓富集量显著低于一次污染26.00%(P<0.05);蚯蚓体腔细胞NRRT平均比一次污染高13.00%(P<0.05);体腔细胞微核率比一次污染低36.10%(P<0.05).本研究表明土壤B[a]P多次叠加污染对蚯蚓的毒性效应低于一次污染,结果可为土壤PAHs类污染物的生态毒性效应评价提供理论基础.

关键词： B[a]P 叠加污染 Tenax提取溶酶体膜微核变率

Primary lateral sclerosis:more than just an upper motor neuron disease

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Neural Regeneration Research 2024年第9期19卷 1881-1882页

作者： Ee Ling tan Jasmin Lope Peter Bede Computational Neuroimaging Group(CNG) School of MedicineTrinity College DublinIreland Department of Neurology St James’s HospitalDublinIreland

Advances in clinical care and recent research achievements:Primary lateral sclerosis(PLS)has traditionally been regarded as a pure upper motor neuron condition,a view perpetuated by most medical textbooks.

关键词： sclerosis clinical traditionally

Resilience conferred by APOE-R136S:a defense bestowed by nature to combat Alzheimer’s disease

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Signal Transduction and Targeted Therapy 2024年第3期9卷 831-832页

作者： Bin Xiao Joshua Kuruvilla Eng-King tan Department of Neurology National Neuroscience InstituteSingaporeSingapore Neuroscience Academic Clinical Program Duke-NUS Medical SchoolSingaporeSingapore Neuroscience and Behavioural Disorders Program Duke-NUS Medical SchoolSingaporeSingapore

In two recent articles published in Nature Neuroscience and Cell,Nelson et al.1 and Chen et al.2 have shown that a rare APOE3 Christchurch(APOE3Ch)variant,R136S,has protective effects on neurodegeneration in various Alzheimer’s disease(AD)model systems through attenuating tau pathology and regulating its related neuroimmune *** et *** Chen et *** provided evidence that identifying the pathophysiologic clues and potential molecular targets from a protective variant such as APOE R136S,can be an attractive viable approach to develop AD therapeutics.

关键词： protective al. Alzheimer

2015—2021年四川盆地近地面O_(3)时空演化及潜在源区分析

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环境科学研究 2023年第4期36卷 660-672页

作者：谭华荣林跃胜姚有如马康方凤满安徽师范大学地理与旅游学院安徽芜湖241003 安徽师范大学江淮流域地表过程与区域响应安徽省重点实验室安徽芜湖241003

近地面O_(3)污染已经成为我国最严重的环境问题之一,特别是在地形相对闭塞的盆地区域更加突出,而盆地地形区域O_(3)的时空演化、潜在源区及驱动因素尚未被完全揭示.因此,以典型盆地地形区—四川盆地为研究区,基于长时间尺度(2015—2021... 详细信息

近地面O_(3)污染已经成为我国最严重的环境问题之一,特别是在地形相对闭塞的盆地区域更加突出,而盆地地形区域O_(3)的时空演化、潜在源区及驱动因素尚未被完全揭示.因此,以典型盆地地形区—四川盆地为研究区,基于长时间尺度(2015—2021年)的O_(3)浓度监测数据,采用后向轨迹、时空地理加权回归等模型探讨O_(3)的时空变化特征、传输路径、潜在源区以及驱动因素的空间分布特征.结果表明:①时间分布上,2015—2021年四川盆地O_(3)-8 h浓度第90百分位数为(143±7)μg/m^(3),夏季O_(3)-8 h浓度高于其他季节,O_(3)-8 h浓度存在明显的“周末效应”和昼夜差异.②空间格局上,四川盆地O_(3)-8 h浓度第90百分位数总体呈西高东低的分布特征,西部平原地带是核心污染区域.③2015—2021年影响成都市的气流轨迹中短距离输送轨迹占比为74.24%,长距离输送轨迹占比平均值为25.76%.2015—2021年成都市O_(3)的潜在源区由西向东迁移,并表现出以盆地为中心逐渐集聚的趋势.④时空地理加权回归结果显示,气温和日照时数的回归系数分别为0.738和0.289,是四川盆地O_(3)的主导自然因子,人口密度(0.412)和人均GDP(0.369)是导致O_(3)浓度升高的主要社会经济因素.研究显示,受盆地内部自然环境和人类活动的共同影响,2015—2021年四川盆地的O_(3)污染较为严重.

关键词：臭氧(O_(3)) 传输路径潜在源区驱动因素四川盆地

A review of in situ carbon mineralization in basalt

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第4期16卷 1467-1485页

作者： Xiaomin Cao Qi Li Liang Xu Yongsheng tan State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China

Global warming has greatly threatened the human living environment and carbon capture and storage(CCS)technology is recognized as a promising way to reduce carbon *** storage is considered a reliable option for long-term carbon *** rich in alkaline earth elements facilitates rapid and permanent CO_(2) fixation as ***,the complex CO_(2)-fluid-basalt interaction poses challenges for assessing carbon storage *** different reaction conditions,the carbonation products and carbonation rates *** mineralization reactions also induce petrophysical and mechanical responses,which have potential risks for the long-term injectivity and the carbon storage safety in basalt *** this paper,recent advances in carbon mineralization storage in basalt based on laboratory research are comprehensively *** assessment methods for carbon storage potential are introduced and the carbon trapping mechanisms are investigated with the identification of the controlling *** in pore structure,permeability and mechanical properties in both static reactions and reactive percolation experiments are also *** study could provide insight into challenges as well as perspectives for future research.

关键词： Carbon mineralization Basalt CO_(2)-fluid-basalt interaction Petrophysical evolution Mechanical response Carbon capture and storage(CCS)