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Effects of docosahexaenoic acid or arachidonic acid supplementation on gene expression and contractile force of rat cardiomyocytes in primary culture

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BIOCELL 2021年第5期45卷 1213-1229页

作者： MIZUNA YANO YUTA UMEHARA TOMOKAZU KUDO TAKAO NAKAMURA tadashi kosawada ATSUYOSHI NISHINA MASAKI SAZUKA DAISUKE SATO ZHONGGANG FENG Graduate School of Science and Engineering Yamagata UniversityYonezawa9928510Japan Graduate School of Medical Science Yamagata UniversityYamagata9909585Japan Department of Materials and Applied Chemistry College of Science and TechnologyNihon UniversityTokyo1018308Japan Department of Health and Nutrition Yamagata Prefectural Yonezawa University of Nutrition SciencesYonezawa9920025Japan

While fatty acids play essential roles in the physiology of the myocardium,conventional culture media contain little *** previously revealed that rat neonatal myocardium mainly contains docosahexaenoic(DHA),linoleic(LA),and arachidonic(AA)acids as polyunsaturated fatty acids(PUFAs),and these contents in cultured cardiomyocytes derived from fetal rats were markedly lower than those in the neonatal *** this study,we first assessed the effects of supplementation of DHA,LA,or AA on the fatty acid contents and the percentage change of contractile area in primarily cultured rat *** on this assessment,we then evaluated the effects of DHA or AA supplementation on mRNA expression and further directly measured the contractile force of cardiomyocytes with the *** study revealed that percentage change of contractile area was maximized under 20μM DHA or 50μM AA supplementation while LA supplementation did not affect this contraction index,and that a widespread upregulation tendency of the mRNA expression related to differentiation,maturity,fatty acid metabolism,and cell adhesion was seen in the cultured cardiomyocytes with supplementation of DHA or *** particular,upregulation of the gene expression of cellular adhesion molecules connexin43 and N-cadherin were remarkable,whereas the effects on differentiation and maturation were less ***,the increase of the percentage change of the contractile area of cardiomyocyte clusters in culture dishes with the supplementations was significant,whereas the enhancement of the contractile force was *** results suggest that supplementation of DHA or AA to the fetal cardiomyocyte culture may play effective roles in preventing the de-differentiation of the cardiomyocytes in culture and that the enhancement of the contractile performance may be mainly attributed to the improvement of intercellular connection.

关键词： Cardiomyocyte culture Polyunsaturated fatty acid mRNA expression Contractile force Intercellular connection

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Refinement of Adaptive Dynamical Simulation of Quantum Mechanical Double Slit Interference Phenomenon

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Journal of Modern Physics 2024年第3期15卷 239-249页

作者： tadashi Ando Andrei Khrennikov Ichiro Yamato Department of Applied Electronics Tokyo University of Science Tokyo Japan International Center for Mathematical Modelling in Physics and Cognitive Sciences Linnaeus University V&aumlxj&oum Sweden Department of Biological Science and Technology Tokyo University of Science Tokyo Japan

We applied adaptive dynamics to double slit interference phenomenon using particle model and obtained partial successful results in our previous report. The patterns qualitatively corresponded well with experiments. Several properties such as concave single slit pattern and large influence of slight displacement of the emission position were different from the experimental results. In this study we tried other slit conditions and obtained consistent patterns with experiments. We do not claim that the adaptive dynamics is the principle of quantum mechanics, but the present results support the probability of adaptive dynamics as the candidate of the basis of quantum mechanics. We discuss the advantages of the adaptive dynamical view for foundations of quantum mechanics.

关键词： Double Slit Interference Adaptive Dynamics Quantum Mechanics Particle Model Simulation

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Proposal of a Method to Easily Understand Rainfall Infiltration in Railway Embankments

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Journal of Civil Engineering and Architecture 2024年第6期18卷 261-268页

作者： Takashi Nakayama tadashi Hara Keigo Koizumi C.S.Inspector Co. Ltd.2-7-2 Nambanaka Naniwa-Ku Osaka-ShiOsaka-Fu 556-0011Japan Research and Education Faculty Kochi University2-5-1 Akebonocho Kochi-ShiKochi-Ken 780-8072Japan Earth Watch Institute Inc.8-10 Toyotsucho Suita-ShiOsaka-Fu 564-0051Japan

Slope failures due to heavy or prolonged rain have been occurring frequently in Japan in recent *** railway embankments,even small-scale surface collapse can result in track ***,train operation during rainy periods is regulated according to empirical rules based on rainfall and disaster ***,the validity of operation regulations is lacking because the rainfall infiltration circumstances inside the slope are *** have been attempting to understand rainfall infiltration in railway embankments by applying a method of predicting surface collapse from observations of volumetric water content in the *** used previous field monitoring and model experiments to propose a method for easily understanding the state of rainfall infiltration in the surface layer of an embankment using the relative history of volumetric water content at different *** this study,we applied this simple determination method to railway embankments with different topography and geological environments to demonstrate the versatility of the method.

关键词： Railway embankments field monitoring volumetric water content

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Effects of elafibranor on liver fibrosis and gut barrier function in a mouse model of alcohol-associated liver disease

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World Journal of Gastroenterology 2024年第28期30卷 3428-3446页

作者： Aritoshi Koizumi Kosuke Kaji Norihisa Nishimura Shohei Asada Takuya Matsuda Misako Tanaka Nobuyuki Yorioka Yuki Tsuji Koh Kitagawa Shinya Sato tadashi Namisaki Takemi Akahane Hitoshi Yoshiji Department of Gastroenterology Nara Medical UniversityKashihara 634-8521Japan

BACKGROUND Alcohol-associated liver disease(ALD)is a leading cause of liver-related morbidity and mortality,but there are no therapeutic targets and modalities to prevent ALD-related liver *** proliferator activated receptor(PPAR)α and δ play a key role in lipid metabolism and intestinal barrier homeostasis,which are major contributors to the pathological progression of ***,elafibranor(EFN),which is a dual PPARαand PPARδagonist,has reached a phase III clinical trial for the treatment of metabolic dysfunctionassociated steatotic liver disease and primary biliary ***,the benefits of EFN for ALD treatment is *** To evaluate the inhibitory effects of EFN on liver fibrosis and gut-intestinal barrier dysfunction in an ALD mouse *** ALD-related liver fibrosis was induced in female C57BL/6J mice by feeding a 2.5% ethanol(EtOH)-containing Lieber-DeCarli liquid diet and intraperitoneally injecting carbon tetrachloride thrice weekly(1 mL/kg)for 8 ***(3 and 10 mg/kg/day)was orally administered during the experimental *** and molecular analyses were performed to assess the effect of EFN on steatohepatitis,fibrosis,and intestinal barrier *** EFN effects on HepG2 lipotoxicity and Caco-2 barrier function were evaluated by cell-based *** The hepatic steatosis,apoptosis,and fibrosis in the ALD mice model were significantly attenuated by EFN *** promoted lipolysis and β-oxidation and enhanced autophagic and antioxidant capacities in EtOH-stimulated HepG2 cells,primarily through PPARα***,EFN inhibited the Kupffer cell-mediated inflammatory response,with blunted hepatic exposure to lipopolysaccharide(LPS)and toll like receptor 4(TLR4)/nuclear factor kappa B(NF-κB)*** improved intestinal hyperpermeability by restoring tight junction proteins and autophagy and by inhibiting apoptosis and proinflammatory *** protective effect on intestinal bar

关键词： Liver fibrosis Ethanol Gut barrier function Apoptosis Autophagy Peroxisome proliferator activated receptor

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The neutrophil–osteogenic cell axis promotes bone destruction in periodontitis

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International Journal of Oral Science 2024年第1期16卷 154-162页

作者： Yutaro Ando Masayuki Tsukasaki Nam Cong-Nhat Huynh Shizao Zang Minglu Yan Ryunosuke Muro Kazutaka Nakamura Masatsugu Komagamine Noriko Komatsu Kazuo Okamoto Kenta Nakano tadashi Okamura Akira Yamaguchi Kazuyuki Ishihara Hiroshi Takayanagi Department of Immunology Graduate School of Medicine and Faculty of MedicineThe University of Tokyo7-3-1HongoBunkyo-kuTokyoJapan Department of Microbiology Tokyo Dental College2-1-14 Kanda-Misaki-choChiyoda-kuTokyoJapan Oral Health Science Center Tokyo Dental College2-9-18Kanda-Misaki-choChiyodakuTokyoJapan Department of Osteoimmunology Graduate School of Medicine and Faculty of MedicineThe University of Tokyo7-3-1HongoBunkyo-kuTokyoJapan Unit of Prosthodontics Laboratory of Oral-Maxillofacial Biology Faculty of Odonto-StomatologyUniversity of Medicine and Pharmacy at Ho Chi Minh CityHo Chi Minh CityVietnam Department of Oral and Maxillofacial Surgery Department of Sensory and Motor System MedicineGraduate School of MedicineThe University of TokyoTokyoJapan Division of Rheumatology Department of Internal MedicineKeio University School of MedicineTokyoJapan Department of Laboratory Animal Medicine Research InstituteNational Center for Global Health and MedicineTokyoJapan

The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease in humans, immune cells accumulate in the oral mucosa and promote bone destruction by inducing receptor activator of nuclear factor-κB ligand (RANKL) expression in osteogenic cells such as osteoblasts and periodontal ligament cells. However, the detailed mechanism underlying immune–bone cell interactions in periodontitis is not fully understood. Here, we performed single-cell RNAsequencing analysis on mouse periodontal lesions and showed that neutrophil–osteogenic cell crosstalk is involved in periodontitis-induced bone loss. The periodontal lesions displayed marked infiltration of neutrophils, and in silico analyses suggested that the neutrophils interacted with osteogenic cells through cytokine production. Among the cytokines expressed in the periodontal neutrophils, oncostatin M (OSM) potently induced RANKL expression in the primary osteoblasts, and deletion of the OSM receptor in osteogenic cells significantly ameliorated periodontitis-induced bone loss. Epigenomic data analyses identified the OSM-regulated RANKL enhancer region in osteogenic cells, and mice lacking this enhancer showed decreased periodontal bone loss while maintaining physiological bone metabolism. These findings shed light on the role of neutrophils in bone regulation during bacterial infection, highlighting the novel mechanism underlying osteoimmune crosstalk.

关键词： period neutrophil destruction

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Positive Effect of Transcranial Direct Current Stimulation on Visual Verbal Working Memory in Patients with Attention-Deficit/Hyperactivity Disorder

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Open Journal of Psychiatry 2024年第4期14卷 334-346页

作者： Tomoko Uchida Daisuke Matsuzawa tadashi Shiohama Katsunori Fujii Akihiro Shiina Masamitsu Naka Katsuo Sugita Eiji Shimizu Naoki Shimojo Hiromichi Hamada Department of Pediatrics Graduate School of Medicine Chiba University Chiba Japan Research Center for Child Mental Development Chiba University Chiba Japan Division of Medical Care and Treatment Center for Forensic Mental Health Chiba University Chiba Japan Department of Cognitive Behavioral Physiology Graduate School of Medicine Chiba University Chiba Japan

Background: Working memory is an executive function that plays an important role in many aspects of daily life, and its impairment in patients with attention-deficit/hyperactivity disorder (ADHD) affects quality of life. The dorsolateral prefrontal cortex (DLPFC) has been a good target site for transcranial direct current stimulation (tDCS) due to its intense involvement in working memory. In our 2018 study, tDCS improved visual-verbal working memory in healthy subjects. Objective: This study examines the effects of tDCS on ADHD patients, particularly on verbal working memory. Methods: We conducted an experiment involving verbal working memory of two modalities, visual and auditory, and a sustained attention task that could affect working memory in 9 ADHD patients. Active or sham tDCS was applied to the left DLPFC in a single-blind crossover design. Results: tDCS significantly improved the accuracy of visual-verbal working memory. In contrast, tDCS did not affect auditory-verbal working memory and sustained attention. Conclusion: tDCS to the left DLPFC improved visual-verbal working memory in ADHD patients, with important implications for potential ADHD treatments.

关键词： Working Memory Attention-Deficit/Hyperactivity Disorder Dorsolateral Prefrontal Cortex Transcranial Direct Current Stimulation

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Non-cooperative optimization games in market-oriented overlay networks： an integrated model of resource pricing and network formation

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Frontiers of Computer Science 2011年第4期5卷 496-505页

作者： Yutaka OKAIE tadashi NAKANO Graduate School of Engineering Osaka University Suita Osaka 565-0871 Japan

In this paper, we formulate a non-cooperative optimization game in market-oriented overlay networks where participating peers share their own computing resources to earn virtual money called energy. We model an overlay network as a set of non-cooperative resource providing peers, called platforms, that perform resource pricing and topology management to maximize their own energy gains. Resource consuming peers, called agents, are simply designed to migrate platform-to-platform to find the least expensive resources in the network. Simulation results are presented to demonstrate the market dynamics as well as the global properties of the network, i.e., resource price and network topology, that emerge from local interactions among the group of peers.

关键词： Overlay networks market mechanisms non-cooperative games mobile agents

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Amelanotic malignant melanoma of the esophagus:Report of two cases with immunohistochemical and molecular genetic study of KIT and PDGFRA

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World Journal of Gastroenterology 2009年第21期15卷 2679-2683页

作者： tadashi Terada Department of Pathology Shizuoka City Shimizu Hospital

The author reports herein two cases of amelanotic malignant melanoma of the esophagus. Case 1 is an 87-year-old woman who was admitted to our hospital because of nausea and vomiting. Endoscopic examination revealed an ulcerated tumor of the distal esophagus, and a biopsy was taken. The biopsy showed malignant polygonal and spindle cells. No melanin pigment was recognized. Immunohistochemically, the tumor cells were positive for melanosome （HMB45）, S100 protein, KIT and Platelet derived growth factor receptor-α （PDG- FRA）. The patient was treated by chemotherapy and radiation, but died of systemic metastasis 12 mo after the presentation. Case 2 is a 56-year-old man presenting with dysphagia. Endoscopic examination revealed a polypoid tumor in the middle esophagus, and a biopsy was obtained. The biopsy showed malignant spindle cells without melanin pigment. Immunohistochemically, the tumor cells were positively labeled for melanosome,S100 protein, KIT and PDGFRA. The patient refused operation, and was treated by palliative chemotherapy and radiation. He died of metastasis 7 mo aEer the admission. In both cases, molecular genetic analyses of gene （exons 9, 11, 13 and 17） and PDGFRA gene （exons 12 and 18） were performed by the PCR direct sequencing method, which showed no mutations of KIT and PDGFRA genes. This is the first report of esophageal malignant melanoma with an examination of the expression of KIT and PDGFRA and the mutational status of K/T and PDGFRA genes.

关键词： Esophagus Amelanotic melanoma KIT Platelet derived growth factor receptor-c~

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Schistosomal appendicitis:Incidence in Japan and a case report

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World Journal of Gastroenterology 2009年第13期15卷 1648-1649页

作者： tadashi Terada Department of Pathology Shizuoka City Shimizu Hospital

Schistosomal appendicitis is very rare in developed countries like the USA,Europe,and *** author reviewed 311 pathologic archival specimens of vermiform appendix over the past 10 *** case of schistosomal appendicitis was ***,the incidence of this disease was 0.32% in all appendices surgically resected in our *** patient was a 41-year-old woman presenting with lower abdominal *** was a sailor traveling to many countries including endemic *** examination,laboratory data,and imaging modalities suggested an acute appendicitis,and appendectomy was performed under the diagnosis of ordinary ***,numerous schistosomal eggs were present in the vasculatures throughout the appendiceal *** of the eggs were *** foreign body reaction was also *** appendicitis was phlegmonous consisting of severe infiltrations of neutrophils and *** serositis was also *** of feces revealed numerous eggs of Schistosoma *** should be aware of schistosomal appendicitis.

关键词： Appendix Histopathology Pathologicarchival specimens Schistosomiasis Acute appendicitis

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Primary multiple extragastrointestinal stromal tumors of the omentum with different mutations of c-kit gene

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World Journal of Gastroenterology 2008年第47期14卷 7256-7259页

作者： tadashi Terada Department of Pathology Shizuoka Municipal Shimizu HospitalMiyakami 1231Shimizu-Ku424-8636ShizuokaJapan

The author reports a very rare case of sporadic primary multiple extragastrointestinal stromal tumors (EGISTs) of the omentum associated with different mutations of the exon 11 of the c-kit gene in a 75-year-old man with gastric cancer. During an operation for the cancer, two solid tumors (10 mm and 8 mm) were found in the omentum. Both tumors consisted of cellular spindle cells. Mitotic figures were two and three per 50 high power fields. The tumor cells were positive for KIT, CD34 and vimentin, but negative for desmin, S100 protein, α-smooth muscle actin and p53 protein. Ki67 labeling was 2% and 3%. The larger EGIST showed a deletion of codons 552-558 of exon 11 of the c-kit gene, while the smaller EGIST had a point mutation at codon 559 (GTT←GAT) in exon 11 of the c-kit gene. Exons 9, 13, and 17 of the c-kit gene, and exons 12 and 18 of the platelet derived growth factor receptor α genes showed no mutations. The case shows that sporadic multiple EGISTs can occur in the omentum.

关键词： Omentum Extragastrointestinal stromal tumors c-kit Platelet derived growth factor receptor CD34

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