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Macroinvertebrate Community Index (MCI) and Quantitative Macroinvertebrate Community Index (QMCI) Analysis: A Comparative Study between Le Afe and Mulivaifagatoloa Rivers, Upolu Island, Samoa

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journal of Geoscience and Environment Protection 2024年第8期12卷 149-167页

作者： S. taupega-Satau P. Amosa A. Leauga j. Nunufolau t. Veni Nun Yan Department of Science National University of Samoa Apia Samoa Office of the Vice Chancellor National University of Samoa Apia Samoa Department of Information technology and Systems National University of Samoa Apia Samoa

the diversity of Samoa’s freshwater macroinvertebrates remains largely unexplored, with past studies focusing on specific species without comprehensive cataloguing. this research evaluated the health of Upolu Island’s rural rivers through macroinvertebrate analysis, particularly in the Le Afe and Mulivaifagatoloa Rivers. Collaborating with Samoa’s Water Resources Division in the Ministry of Natural Resources and Environment (MNRE), three sites along each river were sampled, representing a gradient from pristine to anthropogenically impacted areas. A total of 2953 macroinvertebrates were collected and classified into five categories using established identification keys. the Macroinvertebrate Community Index (MCI) and Quantitative Macroinvertebrate Community Index (QMCI) were applied for analysis. the results showed no clear pattern of pollutant-sensitive species prevalence or decline in less disturbed rivers. High MCI scores with low QMCI values indicated numerous low-scoring species, while the opposite suggested a richness of high-scoring taxa. Although MCI and QMCI are tools for monitoring freshwater health, this study lays the groundwork for future research to categorize Samoan macroinvertebrates and assign tolerance scores based on their presence in varying river conditions. .

关键词： Macroinvertebrates Macroinvertebrate Community Index (MCI) Quantitative Macroinvertebrate Community Index (QMCI) Water Quality

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Considerations for the use of porcine organ donation models in preclinical organ donor intervention research

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Animal Models and Experimental Medicine 2024年第3期7卷 283-296页

作者： Frazer I.Heinis Shaheed Merani Nicholas W.Markin Kim F.Duncan Michael j.Moulton Lance Fristoe William E.thorell Raechel A.Sherrick tami R.Wells Matthew t.Andrews Marian Urban School of Natural Resources Institute of Agriculture and Natural ResourcesUniversity of Nebraska-LincolnLincolnNebraskaUSA Division of transplantation and Vascular Surgery Department of SurgeryUniversity of Nebraska Medical CenterOmahaNebraskaUSA Department of Anesthesiology University of Nebraska Medical CenterOmahaNebraskaUSA Division of Cardiothoracic Surgery Department of SurgeryUniversity of Nebraska Medical CenterOmahaNebraskaUSA Clinical Perfusion Nebraska Medicine-Nebraska Medical CenterOmahaNebraskaUSA Department of Neurosurgery University of Nebraska Medical CenterOmahaNebraskaUSA Nutrition and Health Sciences College of Education and Human SciencesUniversity of Nebraska-LincolnLincolnNebraskaUSA Department of Comparative Medicine University of Nebraska Medical CenterOmahaNebraskaUSA

Use of animal models in preclinical transplant research is essential to the optimization of human allografts for clinical *** models of organ donation and preservation help to advance and improve technical elements of solid organ recovery and facilitate research of ischemia-reperfusion injury,organ preservation strategies,and future donor-based *** considerations include cost,public opinion regarding the conduct of animal research,translational value,and relevance of the animal model for clinical *** present an overview of two porcine models of organ donation:donation following brain death(DBD)and donation following circulatory death(DCD).the cardiovascular anatomy and physiology of pigs closely resembles those of humans,making this species the most appropriate for pre-clinical *** are also considered a potential source of organs for human heart and kidney *** is imperative to minimize animal loss during procedures that are surgically *** present our experience with these models and describe in detail the use cases,procedural approach,challenges,alternatives,and limitations of each model.

关键词： animal model brain death circulatory death organ transplantation

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Leptin Modulates the Differentiation of Keratinocytes via Autophagy in Psoriasis Patients with Metabolic Syndrome

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International journal of Dermatology and Venereology 2024年第3期7卷 121-130页

作者： Song C.-H. Wang R. Zhao Z.-K. Zhang Y. Sun j. Zhang X. Ding X.-Y. Bai j. Liang X.-Q. Wei X.-j. Liu X.-L. Yang t. Liang X.-L. Li C.-X. Lin B.-W. Department of Dermatology PLA General Hospital Beijing 100853 China Department of Clinical Laboratory PLA General Hospital Beijing 100853 China Department of Dermatology Southern Medical University Guangdong Guangzhou 510515 China

Objective: Psoriasis is associated with a high prevalence of metabolic syndrome (MS), and patients with concomitant psoriasis and MS are more severely affected and less responsive to treatment. However, the molecular mechanisms behind these effects are unknown. Recent studies have shown that leptin may serve as a molecular link between psoriasis and MS, suggesting that high leptin concentrations may exacerbate psoriasis. However, the molecular mechanism of this effect is still unclear. We aimed to investigate the effect of leptin on autophagy in patients with psoriasis. Methods: From january 2021 to june 2022 in PLA General Hospital, we enrolled 51 patients with psoriasis, including 21 patients with MS and 30 without MS, and 30 healthy controls who had undergone nevus surgery. We measured the epidermal leptin, P62, and LC3B concentrations of patients by immunohistochemistry, and measured the serum leptin concentration by enzyme-linked immunosorbent assay. We then performed correlation analyses to compare these proteins' concentrations between patients with concomitant psoriasis and MS, patients with psoriasis alone, and healthy control groups. Additionally, we performed western blotting after in vitro culture of HaCat cells with different concentrations of leptin and measured the expression levels of the autophagy markers Beclin1, LC3B, and P62; the differentiation markers K10, K16, and K17; and PI3K/AKt/mtOR signaling pathway-related proteins of HaCat cells. Next, we transfected AtG5 into HaCat cells to revert autophagy and used the specific PI3K inhibitor LY294002 to block PI3K/AKt/mtOR signaling. the expression levels of K10, K16, and K17 of HaCat cells were again measured. One-way analysis of variance was used for the comparison of means of multiple samples, and LSD-t post hoc test was used for comparison between the 2 groups. the counting data were analyzed by the chi-square test. Correlations were evaluated by Pearson correlation analysis. Results: the serum l

关键词： autophagy keratinocyte differentiation leptin metabolic syndrome PI3K/AKt/mtOR psoriasis signal pathway

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On the analytical solution of transient friction in channel flows

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Applied Mathematics and Mechanics(English Edition) 2024年第8期45卷 1447-1466页

作者： F.j.GARCíA-GARCíA P.FARINAS-ALVARINO Department of Naval&Industrial Engineering Polytechnic School of EngineeringUniversity of A CorunaCampus Industrial de FerrolFerrol 15403A CorunaSpain Integraciones técnicas de Seguridad S.A.Pol.Ind.Espiritu SantoC/Nobel15Cambre 15650A CorunaSpain

the transient friction in channel mean flows is the sum of two contributions,i.e.,the underlying laminar flow(ULF)and the purely turbulent component(PtC),and the contributions are analyzed separately by theoretical *** is found that,the transient friction may be higher or remarkably lower than that in equal-Reynolds number steady-state *** universal time constant for plane-parallel laminar flows is reported,and the role of the time constant in a turbulent mean flow is *** is shown that the time constant is related to the turbulence's frozen ***,a study of the logarithmic layer during the transient flow is accomplished,which shows that the logarithmic layer is destroyed.

关键词： transient friction transient flow unsteady channel flow

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Genotypes and Phenotypes of Uygur Children with Xeroderma Pigmentosum: A Case Study in Xinjiang, China

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International journal of Dermatology and Venereology 2024年第3期7卷 144-149页

作者： Qiu Y. Wang W.-j. Liang j.-Q. Yakeya B. Wang P. Zhang j.-Z. Kang X.-j. Department of Dermatology and Venereology People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang Uygur Autonomous Region Urumqi 830001 China Xinjiang Clinical Research Center for Dermatologic Diseases Xinjiang Uygur Autonomous Region Urumqi 830001 China Xinjiang Key Laboratory of Dermatology Research (XjYS1707) Xinjiang Uygur Autonomous Region Urumqi 830001 China

Objective: Xeroderma pigmentosum (XP) is a rare autosomal recessive dermatosis caused by genetic defects of DNA repair. this study was performed to detect and analyze the genes of 2 Uygur patients with XP and their families and assess the patients' phenotypes, which may enrich the understanding of the genetic skin disorder spectrum in Xinjiang area. Methods: We collected the clinical data from 2 patients with XP and peripheral blood samples from the patients and their family members. the patients' DNA was sequenced and detected by Sanger sequencing, and gene mutations were screened. Results: the proband in family 1 presented with brown maculae at the exposure site and squamous cell carcinoma secondary to a facial rash. the proband had a homozygous nucleotide variation of XPC c.2251-2A> G (A change from A to G in the penultimate position of the intron before the 2251 position in the coding region), which was a shear mutation. In this family, both parents were heterozygous, and no similar mutation detected in the sister. In family 2, the proband had scattered black brown spots and papules on the trunk and limbs. and his younger sister was also a patient. the proband and his younger sister had homonucleotide variation of XPA c.631C> t, which was nonsense mutation, resulting in the codon for Arg No.211 being changed into termination codon (***211X), thus terminating the peptide chain synthesis prematurely. All the normal individuals in the two families were heterozygotes, and homozygous mutations occurred in all the patients, which was consistent with the autosomal recessive inheritance. Conclusion: XP is rare in Uygur population. this study expanded the mutation spectrum of XP and provided a basis for early diagnosis, treatment, prognostic prediction, and prenatal genetic consultation. © 2022 Hospital for Skin Diseases (Institute of Dermatology), Chinese Academy of Medical Sciences, and Chinese Medical Association, published by Wolters Kluwer, Inc.

关键词： gene mutation xeroderma pigmentosum XPA gene XPC gene

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Experimental Investigations of Quasi-Coherent Micro-Instabilities in j-tEXt Ohmic Plasmas

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Chinese Physics Letters 2024年第5期41卷 47-52页

作者：石鹏庄革程芝峰高丽周乙楠刘永罗景庭李景春 Institute of Plasma Physics Chinese Academy of SciencesHefei 230031China International joint Research Laboratory of Magnetic Confinement Fusion and Plasma Physics State Key Laboratory of Advanced Electromagnetic Engineering and TechnologySchool of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430074China Department of Engineering and Applied Physics School of Physical Sciences University of Science and Technology of ChinaHefei 230026China Shenzhen Key Laboratory of Nuclear and Radiation Safety Institute for Advanced Study in Nuclear Energy&SafetyCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 510640China

Quasi-coherent micro-instabilities is one of the key topics of magnetic confinement fusion. this work focuses on the quasi-coherent spectra of ion temperature gradient(ItG) and trapped-electron-mode instabilities using newly developed far-forward collective scattering measurements within ohmic plasmas in the j-tEXt *** ItG mode is characterized by frequencies ranging from 30 to 100 k Hz and wavenumbers(k_(θρs)) less than 0.3. Beyond a critical plasma density threshold, the ItG mode undergoes a bifurcation, which is marked by a reduction in frequency and an enhancement in amplitude. Concurrently, enhancements in ion energy loss and degradation in confinement are observed. this ground-breaking discovery represents the first instance of direct experimental evidence that establishes a clear link between ItG instability and ion thermal transport.

关键词： ItG mode collective

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Metabolic syndrome and the urinary microbiome of patients undergoing percutaneous nephrolithotomy

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Asian journal of Urology 2024年第2期11卷 316-323页

作者： Ryan A.Dornbier Chirag P.Doshi Shalin C.Desai Petar Bajic Michelle Van Kuiken Mark Khemmani Ahmer V.Farooq Larissa Bresler thomas M.t.turk Alan j.Wolfe Kristin G.Baldea Loyola University Medical Center Department of UrologyMaywoodILUSA Loyola University Chicago Department of Microbiology and ImmunologyMaywoodILUSA

Objective:to identify possible stone-promoting microbes,we compared the profiles of microbes grown from stones of patients with and without metabolic syndrome(MetS).the association between MetS and urinary stone disease is well established,but the exact pathophysiologic relationship remains *** evidence suggests urinary tract dysbiosis may lead to increased nephrolithiasis ***:At the time of percutaneous nephrolithotomy,bladder urine and stone fragments were collected from patients with and without *** sample types were subjected to expanded quantitative urine culture(EQUC)and 16 S ribosomal RNA gene ***:Fifty-seven patients included 12 controls(21.1%)and 45 MetS patients(78.9%).Both cohorts were similar with respect to demographics and non-MetS *** controls had uric acid stone *** EQUC,bacteria were detected more frequently in MetS stones(42.2%)compared to controls(8.3%)(p=0.041).Bacteria also were more abundant in stones of MetS patients compared to *** validate our EQUC results,we performed 16 S ribosomal RNA gene *** 12/16(75.0%)sequence-positive stones,EQUC reliably isolated at least one species of the sequenced *** were detected in both“infectious”and“non-infectious”stone ***:Bacteria are more common and more abundant in MetS stones than control *** findings support a role for bacteria in urinary stone disease for patients with MetS regardless of stone composition.

关键词： Nephrolithiasis Urolithiasis Metabolic syndrome Urinary microbiome Percutaneous nephrolithotomy

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On an isotropic porous solid cylinder:the analytical solution and sensitivity analysis of the pressure

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Applied Mathematics and Mechanics(English Edition) 2024年第9期45卷 1499-1522页

作者： H.ASGHARI L.MILLER R.PENtA j.MERODIO Departamento de Matematica Aplicada a las tIC ETS de Ingenieria de Sistemas InformaticosUniversidad Politecnica de MadridMadrid 28031Spain School of Mathematics and Statistics University of GlasgowGlasgow G128QQU.K.

Within this work,we perform a sensitivity analysis to determine the influence of the material input parameters on the pressure in an isotropic porous solid *** provide a step-by-step guide to obtain the analytical solution for a porous isotropic elastic cylinder in terms of the pressure,stresses,and elastic *** obtain the solution by performing a Laplace transform on the governing equations,which are those of Biot's poroelasticity in cylindrical polar *** enforce radial boundary conditions and obtain the solution in the Laplace transformed domain before reverting back to the time *** sensitivity analysis is then carried out,considering only the derived pressure *** analysis finds that the time t,Biot's modulus M,and Poisson's ratio ν have the highest influence on the pressure whereas the initial value of pressure P_(0) plays a very little role.

关键词： sensitivity analysis Laplace transform cylindrical polar coordinate Biot's modulus cylinder

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Attitude control of multi-spacecraft systems on SO(3)with stochastic links failure

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Chinese journal of Aeronautics 2024年第3期37卷 320-331页

作者： KANG Zeyu SHEN Qiang WU Shufan Chris j.DAMAREN MU Zhongcheng School of Aeronautics and Astronautics Shanghai Jiao Tong UniversityShanghai 200240China Institute for Aerospace Studies University of TorontoTorontoOntario M3H 5T6Canada

In this paper,for Multi-Spacecraft System(MSS)with a directed communication topology link and a static virtual leader,a controller is proposed to realize attitude consensus and attitude stabilization with stochastic links failure and actuator ***,an MSS attitude error model suitable for a directed topology link and with a static virtual leader based on SO(3)is derived,which considers that the attitude error on SO(3)cannot be defined based on algebraic ***,we design a controller to realize the MSS on SO(3)with attitude consensus and attitude stabilization under stochastic links failure and actuator ***,the simulation results of a multi-spacecraft system with stochastic links failure and a static virtual leader spacecraft are demonstrated to illustrate the efficiency of the attitude controller.

关键词： Multi-spacecraft systems Attitude consensus Attitude stabilization Stochastic links failure Super-martingale convergence

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Rapid Green Synthesis of Silver Nanoparticles by Reishi and their Antibacterial Activity and Mechanisms

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journal of Biomaterials and Nanobiotechnology 2024年第3期15卷 51-63页

作者： Akamu j. Ewunkem t’nasia Priester Desia Williams Bailey Ariyon Ilunga tshimanga Brittany justice Dinesh K. Singh Department of Biological Sciences Winston-Salem State University Winston-Salem North Carolina USA

Nanotechnology is a rapidly growing field in biomedical engineering with references to efficiency, safety, and cost-effective approaches. Herein, the objective of this study was to examine an innovative approach to rapidly synthesis silver nanoparticles from an aqueous extract of medicinal mushroom Ganoderma lucidum (also known as reishi). the structural and dimensional dispersion of the biosynthesized silver nanoparticles from reishi was confirmed by UV-Vis spectrophotometer (UV-Vis) and Scanning Electron Microscopy (SEM) analysis. Additionally, the biosynthesized silver nanoparticles from resihi were used to explore their potential antimicrobial activity against Staphylococcus aureus and Micrococcus luteus and Escherichia coli and Klebsiella pneumoniae. the results from this study revealed that the silver nanoparticles mediated by reishi adopted a spherical shape morphology with sizes, less than 100 nm and revealed strong absorption plasmon band at 440 nm. Furthermore, the biosynthesized silver nanoparticles from reishi exhibited antibacterial activity against the tested S. aureus and M. luteus and E. coli and K. pneumoniae by altering their morphology which signifies their biomedical potential.

关键词： Nanotechnology Reishi Nanoparticles Antimicrobial Green Synthesis

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