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柑桔胚愈伤组织原生质体的植株分化

国外农学（果树） 1990年第4期 20-23页

作者： t.hidaka I.Kajiura 叶林菲

将3种柑桔:华盛顿脐橙、柚柑(*** ***)掽柑(*** Blanco)幼嫩种子的胚接种在含有0.16M蔗糖、0.5g/1麦芽抽提物和50μ激动素的MS培养基上,1—3个月后,白色松脆的愈伤组织以下胚轴产生,继代培养2年左右,其形态即具成胚力。由愈伤组织分离... 详细信息

将3种柑桔:华盛顿脐橙、柚柑(*** ***)掽柑(*** Blanco)幼嫩种子的胚接种在含有0.16M蔗糖、0.5g/1麦芽抽提物和50μ激动素的MS培养基上,1—3个月后,白色松脆的愈伤组织以下胚轴产生,继代培养2年左右,其形态即具成胚力。由愈伤组织分离出的原生质体,在所供试的培养基中都产生胚细胞团,含有1μM玉米素或不含任何生长调节剂的培养基中,也有球形和鱼雷形胚状体产生,其中大部分移檀在古有1μMGA_6和0.06M蔗糖的MS琼脂培养基上后。再生长成完整植株。

关键词：柑桔胚状体原生质体植株分化

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日本高强度声试验设备

导弹与航天运载技术 1983年第1期 88-94页

作者： t.hidaka S.takagi M.Ueda 朱仲方

引言火箭和卫星发射之前,通常要进行如温度、湿度、振动和冲击等环境试验,以改进整个系统的可靠性。高强度的声试验即是其中的一种。国外,已经设计和建成了几种高强度声试验设备,并成功地投入了使用。火箭在起飞和最大动压飞行时会遇到... 详细信息

引言火箭和卫星发射之前,通常要进行如温度、湿度、振动和冲击等环境试验,以改进整个系统的可靠性。高强度的声试验即是其中的一种。国外,已经设计和建成了几种高强度声试验设备,并成功地投入了使用。火箭在起飞和最大动压飞行时会遇到严重的声环境。起飞时,噪声源就在火箭发动机的超音速排气流中。飞行期间,火箭外蒙皮紊流附面层里发生的压力脉动在最大动压时非常大。早期的高强度声试验利用火箭发动机和喷气发动机地面静态试验来模拟这些声环境。而

关键词：混响室声功率换能器声学实验室传感器测深仪倍频带倍频程最大声压级高强度试验设备实验室设备日本

Enzyme-linked immunosorbent assay of 3 Screen Islet Cell Autoantibody in patients with autoimmune thyroid disease

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World Journal of Diabetes 2024年第5期15卷 935-944页

作者： Eiji Kawasaki Hidekazu tamai takahiro Fukuyama Yoko Sagara Ryutaro hidaka Aira Uchida Masayuki tojikubo Narihito tatsumoto Yuko Akehi Yuji Hiromatsu the Diabetes Thyroid and Endocrine CenterShin-Koga HospitalKurume 830-8577Japan

BACKGROUND In recent years,the emergence of multiplex technology that can simultaneously measure multiple anti-islet autoantibodies has become particularly valuable for the staging and early diagnosis of immune-mediated type 1 diabetes(t1D).While it has been established that 20%-30%of t1D patients suffer from autoimmune thyroid disease(AItD),there is limited available data regarding the presence of anti-islet autoantibodies in AItD *** commercially available anti-islet autoantibodies,glutamic acid decarboxylase 65 autoantibodies(GADAs)are often the first marker measured in general clinical *** to investigate the frequency of anti-islet autoantibodies in AItD *** Our study involved four hundred ninety-five AItD patients,categorized into three distinct groups:AItD with t1D(n=18),AItD with phenotypic type 2 diabetes(t2D)(n=81),and AItD without diabetes(n=396),and the enzyme-linked immunosorbent assay(ELISA)was employed to determine the frequencies of 3 Screen Islet Cell Autoantibody(3 Screen ICA),GADA,insulinoma-associated antigen-2 autoantibodies(IA-2As),and zinc transporter 8 autoantibodies(Znt8As)within these *** the frequency of 3 Screen ICA in AItD patients with t1D,t2D,and those without diabetes were 88.9%,6.2%,and 5.1%,respectively,with no significant difference seen between the latter two ***,the frequency of 3 Screen ICA was 11.1%higher in AItD patients with t1D,1.3%higher in AItD patients with t2D,and 1.1%higher in AItD patients without diabetes compared to GADA,***,12.5%,20.0%,and 20.0%of the 3 Screen ICA-positive patients were negative for ***,1.3%of the AItD patients who tested negative for 3 Screen ICA in both the AItD with t2D and non-diabetic AItD groups were found to be positive for individual *** the 3 Screen ICA-positive patients,there was a significantly higher proportion of individuals with multiple autoantibodies in AItD patients with t1D com

关键词： Anti-islet autoantibodies Autoimmune thyroid disease Real-world practice Retrospective study type 1 diabetes

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从特罗维塔甜橙的花药诱导小植株

国外农学(果树) 1985年第4期 43-44页

作者： hidaka t. 张进仁

引言像果树这种多年生植物的育种需要很长的时间和广大的空间,因为它们是高度杂合体、童期长和有大的植物体。因此,特别是对于这些种,纯合的植株对植物育种和作物改良将有重要的价值。为了得到纯系,正在开展许多种的花药培养技术研究,然... 详细信息

引言像果树这种多年生植物的育种需要很长的时间和广大的空间,因为它们是高度杂合体、童期长和有大的植物体。因此,特别是对于这些种,纯合的植株对植物育种和作物改良将有重要的价值。为了得到纯系,正在开展许多种的花药培养技术研究,然而,在果树方面只有几篇报导。自从hidaka和他的合作者报导了枳的花药诱导单倍体植株以来,又报导了一些关于柑桔花药培

关键词：特罗维塔甜橙酸橙胚状体小植株花药培养生长调节剂

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原发性输卵管腺癌脑转移

世界核心医学期刊文摘（妇产科学分册） 2005年第2期1卷 56-57页

作者： hidaka t. Nakamura t. Shima t. 郭慧芳

Primary adenocarcinoma of the fallo pian tube is a rare disease,and cerebral metastasis fr om this tumor is an ex-tremely rare event.A 61-year -old fe male,who had been thought to be in a disease -free s tate after the initial treatment for stage IIIc fallopian t ube adenocarcinoma,presented with severe headache,diz ziness,nausea,and *** Ct and MRI revealed s olitary metastatic lesion in the cerebrum,which was the only site of *** was removed surgically,fo llowed by platinum -based combination chemotherapy,an d a clinical remission was achieved for *** n should be paid to the possibility of cerebralmetasta sis in patients who develop neurogenic signs and *** survival could be achieved with aggressive treatme nt containing surgical debulking and adjuvant chemotherap y for solitary cere-bralmetastasis.

关键词：脑转移顺铂化疗临床缓解期辅助化疗生存时间初次治疗侵入性治疗

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troglitazone prevented the development of fatty liver under obese and diabetic condition in Otsuka Long-Evans tokushima fatty rats

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中国病理生理杂志 2001年第8期 70-70页

作者： H.Sakino M.Ookuma S.Fukuchi S.hidaka H.Yoshimatsu t.Sakata t.Okeda Department of Internal Medicine I Gerontological Nursing Oita Medical University Oita Japan

We aimed to clarify the effects of troglitazone to prevent the development of fatty liver in Otsuka Long-Evans tokushima fatty (OLEtF) rats, an obese type2 diabetes model. treatment of 0.2% troglitazone for 16 weeks significantly decreased blood glucose, serum insulin and free fatty acid (FFA) concentration (P＜0.05 for each) in the OLEtF rats leaving their food intake, body weight and general fat pad weight unaffected compared with those of their lean littermates, Long-Evans tokushima Otsuka (LEtO) rats. troglitazone restored increase in liver weight and hepatic triglyceride(tG) content of the OLEtF rats (P＜0.05 for each). these findings were accertained by histological examination which showed that fat deposition, necrosis and vacuolization of hepatocyte, markers for fatty liver, were diminished by troglitazone treatment. Messenger RNA expression of microsomal triglyceride transfer protein in the liver, a marker for hepatic tG output, was not significantly different between OLEtF and LEtO rats with or without troglitazone treatment. Fatty acid composition such as C18∶1(oleic acid) showed no remarkable change after troglitazone treatment in OLEtF rats, indicating no dietary influence to hepatic lipogenesis. these indicate that reduction in circulating FFA level may be one of the main mechanisms for troglitazone to prevent the development of insulin resistance and fatty liver.

关键词： Long

A Study of SIC-Nanoparticles Porous Layer Formed on SIC-DPF Wall for Soot Oxidation

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A Study of SIC-Nanoparticles Porous Layer Formed on SIC-DPF ...

Prion Protein Binds to Aldolase A Produced by Bovine Intestinal M Cells

the FISItA 2012 World Automotive Congress--Volume 1：Advanced Internal Combustion Engines

作者： Keita Ishizaki Shinichi tanaka Atsushi Kishimoto Masamichi tanaka Naoki Ohya Nobuhiro hidaka Automobile R&D Center.Honda R&D Co. LtdTochigiJapan New technology Research Laboratory.Sumitomo Osaka Cement Co. LtdChibaJapan

the pore structure of DPF is known to have a significant effect on filtration efficiency,pressure drop,and soot *** is reported that the formation of a nanoparticle porous layer on the surface of the DPF inlet wall promotes soot oxidation[1-3].However,the correlation between pore size in the nanoparticle layer and soot oxidation performance,the effect of the layer in actual use environments,and the oxidation mechanism involved have not yet been *** research discussed in this paper therefore formed SiC nanoparticle porous layers of various pore sizes on the inlet wall surfaces of SiC-DPF(creating what will be termed"NPLDPF"here)using the dip-coating method|4|.the NPL thickness was set at 20μm to help enable uniform coating samples to be *** regard to filter pressure drop without soot loading,results for a bare DPF and a Pt/γ-AlO-eoated DPF(catalyzed DPF:Pt=1 g/L)were virtually *** the NPL-DPF,pressure drop increased as pore size was *** contrast,in the case of pressure drop following soot loading(3 g/L).despite the fact that results for pressure drop for the NPL-DPF were higher than those for the bare DPF,they were lower than those for the catalyzed *** is possible to stop deep-bed filtration by forming a nanoparticle porous layer on the surface of the DPF inlet wall[1.5].With regard to soot oxidation performance,the t80 of an NPL-DPF(NPL pore size=350 nm)was found to be40%lower than that of a bare DPF and identical to that of a catalyzed *** addition,reducing the NPL pore size enhanced soot oxidation performance.X-ray photoelectron spectra(XPS),transmission electron microscopy(tEM),andhydrogen temperature-programmed desorption(H2-tPD)results indicated that the vSiC nanoparticle surface was covered by a 5 am SiO oxide *** was found that these oxidized SiC nanoparticles desorbed oxygen at around 450℃.this desorbed oxygen promoted the oxidation of diesel *** addition,the filtration efficiency of the NPL-DPF wi

关键词： Diesel vehicle Partieulate-emissions Paniculate filters Sootoxidation Desorhed oxygen

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Open Journal of Veterinary Medicine 2015年第3期5卷 43-60页

作者： Yuya Nagasawa Yu takahashi Wataru Itani Hitoshi Watanabe Yusuke hidaka Shotaro Morita Kei Suzuki Kouichi Watanabe Shyuichi Ohwada Haruki Kitazawa Morikazu Imamura takashi Yokoyama Motohiro Horiuchi Suehiro Sakaguchi Shirou Mohri Michael t. Rose tomonori Nochi Hisashi Aso Cellular Biology Laboratory Tohoku University Sendai Japan Food Immunology Group Tohoku University Sendai Japan Prion Disease Research Center National Institute of Animal Health Tsukuba Japan Laboratory of Veterinary Hygiene Hokkaido University Sapporo Japan Institute for Enzyme Research The University of Tokushima Tokushima Japan Institute of Biological Environmental and Rural Sciences Aberystwyth University Aberystwyth UK

Microfold (M) cells are a kind of intestinal epithelial cell in the follicle-associated epithelium (FAE) of Peyer’s patches. they can transport antigens and microorganisms to lymphoid tissues. Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disorder in cattle. It is linked to variant Creutzfeldt-Jakob disease in humans. Although it is thought that M cells transport the BSE agent, the exact mechanism by which it crosses the intestinal barrier is not clear. We have bovine intestinal epithelial cell line (BIE cells), which can differentiate into the M cell type in vitro after stimulation, and which is able to transport the BSE agent. We show here that M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. the results of 2-DE show a specific protein associated with the PrP-coated particles, compared with non-coated particles. this protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. the binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Aldolase A was co-stained with incorporated PrPSc in M-BIE cells. these results suggest that bovine M cells and goblet cells synthesize aldolase A, and that aldolase A may have the ability to bind PrP and associate with PrP in cellular vesicles. therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.

关键词： Peyer’s Patch M Cell BIE Cells Aldolase A PrP Binding Protein

联合血流介导的血管舒张和硝酸甘油诱导的血管舒张能更有效预测心血管事件

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中华高血压杂志 2016年第4期24卷 379-379页

作者：刘莉叶鹏 Kajikawa M Maruhashi t Hida E Iwamoto Y Matsumoto t Iwamoto A Oda N Kishimoto S Matsui S hidaka t Kihara Y Chayama K Goto C Aibara Y Nakashima A Noma K Higashi Y

硝酸甘油诱导的血管舒张作为血流介导的血管舒张功能（flow-mediated vasodilation,FMD）的对照试验,用来区别内皮依赖性与非依赖性的血管舒张功能。近来,硝酸甘油诱导的血管舒张已被报道是动脉粥样硬化分级的有用标志物。该研究旨在评... 详细信息

硝酸甘油诱导的血管舒张作为血流介导的血管舒张功能（flow-mediated vasodilation,FMD）的对照试验,用来区别内皮依赖性与非依赖性的血管舒张功能。近来,硝酸甘油诱导的血管舒张已被报道是动脉粥样硬化分级的有用标志物。该研究旨在评估联合FMD与硝酸甘油诱导的血管舒张对未来心血管事件的预测价值。方法与结果：纳入研究对象402例,其中包括心血管病患者,测定FMD与硝酸甘油诱导的血管舒张。

关键词：血管舒张心血管事件心源性死亡心血管病患者血流介导内皮依赖性预测价值非依赖性急性心肌梗死特征曲线

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