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A Single-tube, Functional Marker-Based Multiplex PCR Assay for Simultaneous Detection of Major Bacterial Blight Resistance Genes Xa21, xa13 and xa5 in Rice

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Rice science 2016年第3期23卷 144-151页

作者： S.K. Hajira R.M. Sundaram G.S.Laha A.Yugander S.M.Balachandran B.C.Viraktamath K.Sujatha C.H.Balachiranjeevi K.Pranath M.Amla S.Bhaskar V.Abhilash H.K.Mahadevaswamy M.Kousik t.dilipkumar G.Harika G.Rekha ICAR-Indian Institute of Rice Research Rajendranagar Hyderabad 500030 India

In marker-assisted breeding for bacterial blight(BB) resistance in rice, three major resistance genes, viz., Xa21, xa13 and xa5, are routinely deployed either singly or in combinations. As efficient and functional markers are yet to be developed for xa13 and xa5, we have developed simple PCR-based functional markers for both the genes. For xa13, we designed a functional PCR-based marker, xa13-prom targeting the In Del polymorphism in the promoter of candidate gene Os8N3 located on chromosome 8 of rice. With respect to xa5, a multiplex-PCR based functional marker system, named xa5 FM, consisting of two sets of primer pairs targeting the 2-bp functional nucleotide polymorphism in the exon II of the gene tFIIA5(candidate for xa5), has been developed. Both xa13-prom and xa5 FM can differentiate the resistant and susceptible alleles for xa13 and xa5, respectively, in a co-dominant fashion. Using these two functional markers along with the already reported functional PCR-based marker for Xa21(p tA248), we designed a single-tube multiplex PCR based assay for simultaneous detection of all the three major resistance genes and demonstrated the utility of the multiplex marker system in a segregating population.

关键词： rice bacterial blight resistance xa5 xa13 Xa21 functional marker multiplex PCR

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Characterization and screening of in vitro antimalarial and larvicidal activities of selected seaweeds from southeast coast of India against Plasmodium falciparum andAnopheles stephensi

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Journal of Coastal Life Medicine 2017年第6期5卷 242-248页

作者： Rajamani Sowmiya Govindasamy Balasubramani Paramasivam Deepak dilipkumar Aiswarya Sundaram Ravikumar SundaramPrasannakumar Pachiappan Perumal Department of Biotechnology School of BiosciencesPeriyar UniversityPeriyar Palkalai NagarSalemTamil Nadu636011India Department of Oceanography and Coastal Area Studies School of Marine SciencesAlagappa UniversityThondi CampusRamanathapuramTamil Nadu623409India

Objective:to investigate the antimalarial and larvicidal activities of seaweeds against Plasmodium falciparum strain andAnopheles stephensi mosquito larva. Methods: the three different seaweeds,Jania rubens,turbinaria ornata (t. ornata) and Ulva fasciata were collected from Southeast India. the seaweeds were extracted using hexane and ethyl acetate solvents and evaluated for their mosquito-larvicidal and antiplasmodial property. the bioactive compounds of the seaweed extract were studied by Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. Results:the hexane extract oft. ornata (HtO) has shown significant inhibitory effects at the IC50 value 22.98 μg/mL, when compared to the positive control, chloroquine (12.32 μg/mL). the larvicidal activity of HtO on the fourth instar larvae of Anopheles stephensi was found to be effective with the LC50 and LC90 values (μg/mL) of 1.38 and 3.34. the Fourier transform infrared spectroscopy analysis ofHtO revealed the presence of alkenes, aromatics, amides and amines groups, whereas in gas chromatography-mass spectrometry, 11 different bioactive components were recorded. the bioactive component, hentriacontane in the HtO might be responsible for the recorded antiplasmodial and larvicidal activities. Conclusions:the finding of the present study reveals that the constituents of t. ornata have new sources of antimalarial and larvicidal agents in future.

关键词： Anopheles stephensi Ft-IR GC-MS Plasmodium falciparum turbinaria ornata

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