检索结果-南通市图书馆

Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John's wort

引用

Molecular Plant 2024年第9期17卷 1439-1457页

作者： Song Wu Ana Luisa Malaco Morotti Jun Yang Ertao Wang Evangelos c.tatsis National Key Laboratory of Plant Molecular Genetics CASs Center for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChineseAcademy of SciencesShanghai 200032China University of chinese Academy of Sciences Shanghai 200032China UEPAMS-cAS-JIcs centre of Excellencefor Plant anamlcroblalSclence Snangnal 200032Cnina

Hyperforin is the compound responsible for the effectiveness of ***'s wort(Hypericum perforatum)as an antidepressant,but its complete biosynthetic pathway remains *** discovery based on co-expression analysis of bulk RNA-sequencing data or genome mining failed to discover the missing steps in hyperforin *** this study,we sequenced the 1.54-Gb tetraploid *** genome assem-bled into 32 chromosomes with the scaffold N50 value of 42.44 *** single-cell RNA sequencing,we iden-tified a type of cell,“Hyper cells”,wherein hyperforin biosynthesis de novo takes place in both the leaves and *** pathway reconstitution in yeast and tobacco,we identified and characterized four transmembrane prenyltransferases(HpPt1-4)that are localized at the plastid envelope and complete the hyperforin biosynthetic *** hyperforin polycyclic scaffold is created by a reaction cascade involving an irregular isoprenoid coupling and a tandem *** findings reveal how and where hy-perforin is biosynthesized,enabling synthetic-biology reconstitution of the complete ***,this study not only deepens our comprehension of specialized metabolism at the cellularlevel but also provides strategic guidance for elucidation of the biosynthetic pathways of other specializied metabolites in plants.

关键词： hyperforin Hypericum perforatum de novo genome sequencing single-cell RNA sequencing biosyn-thetic pathway meroterpenoids

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Forecasting of tropical cyclones ASANI (2022) and MOcHA (2023) over the Bay of Bengal - real time challenges to forecasters

引用

tROPIcAL cYcLONE RESEARcH AND REVIEW 2024年第2期13卷 88-112页

作者： Kotal, S. D. Arulalan, t. Mohapatra, M. Govt India Minist Earth Sci India Meteorol Dept New Delhi India

this study examines the track and intensity forecasts of two typical Bay of Bengal tropical cyclones (tc) ASANI and MOcHA. the analysis of various Numerical Weather Prediction (NWP) model forecasts [EcMWF (European centre for Medium range Weather Forecast), NcEP (National centers for Environmental Prediction), NcUM (National centre for Medium Range Weather Forecast-Unified Model), IMD (India Meteorological Department), HWRF (Hurricane Weather Research and Forecasting)], MME (Multi-model Ensemble), ScIP (Statistical cyclone Intensity Prediction) model, and OFcL (Official) forecasts shows that intensity forecasts of ASANI and track forecasts of MOcHA were reasonably good, but there were large errors and wide variation in track forecasts of ASANI and in intensity forecasts of MOcHA. Among all model forecasts, the track forecast errors of IMD model and MME were least in general for ASANI and MOcHA respectively. Also, the landfall point forecast errors of IMD were least for ASANI, and the MME and OFcL forecast errors were least for MOcHA. No model is found to be consistently better for landfall time forecast for ASANI, and the errors of EcMWF, IMD and HWRF were least and of same order for MOcHA. the intensity forecast errors of OFcL and ScIP were least for ASANI, and the forecast errors of HWRF, IMD, NcEP, ScIP and OFcL were comparable and least for MOcHA up to 48 h forecast and HWRF errors were least thereafter in general. the EcMWF model forecast errors for intensity were found to be highest for both the tcs. the results also show that although there is significant improvement of track forecasts and limited or no improvement of intensity forecast in previous decades but challenges still persists in real time forecasting of both track and intensity due to wide variation and inconsistency of model forecasts for different tc cases. (c) 2024 the Shanghai typhoon Institute of china Meteorological Administration. Publishing services by Elsevier B.V. on behalf of KeAi communi

关键词： tropical cyclone track forecast Intensity forecast NWP model Bay of Bengal North Indian Ocean NORtH INDIAN-OcEAN INtENSItY PREDIctION AtLANtIc BASIN tRAcK FOREcASt LANDFALL PAcIFIc MODEL EYE

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Peptide photocaging:A brief account of the chemistry and biological applications

引用

chinese chemical Letters 2018年第7期29卷 1058-1062页

作者： Wing Ho So clarence t.t.wong Jiang Xia Department of chemistry The Chinese University of Hong Kong Hong Kong Special Administrative Region

Light is arguably the most convement non-invasive stimulant to perturb or control specinc cnemical reactions in the biological systems. Upon light illumination, photosensitive molecules incur conformational changes or formation/breaking of chemical bonds. consequently, these molecules can be used to transfer signals from one location to another in the cell, from outside of the cell to the inside, or from a light bulb to the interior of animal tissues. the development of the photochemical reactions of organic compounds has paved the road towards their use in peptides and peptide-based biological applications. In this mini-review, we summarized the state-of-the-art development of photo-protecting groups for peptide photocaging including the un-caging mechanism of different PPGs, the synthesis of photo-caged peptides, and the recent applications of peptide photocaging in chemical biology.

关键词： Photo-protecting groups Photocaged peptides Photocaged proteins Photodecaging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Leptin Modulates the Differentiation of Keratinocytes via Autophagy in Psoriasis Patients with Metabolic Syndrome

引用

International Journal of Dermatology and Venereology 2024年第3期7卷 121-130页

作者： Song c.-H. Wang R. Zhao Z.-K. Zhang Y. Sun J. Zhang X. Ding X.-Y. Bai J. Liang X.-Q. Wei X.-J. Liu X.-L. Yang t. Liang X.-L. Li c.-X. Lin B.-W. Department of Dermatology PLA General Hospital Beijing 100853 China Department of clinical Laboratory PLA General Hospital Beijing 100853 China Department of Dermatology Southern Medical University Guangdong Guangzhou 510515 China

Objective: Psoriasis is associated with a high prevalence of metabolic syndrome (MS), and patients with concomitant psoriasis and MS are more severely affected and less responsive to treatment. However, the molecular mechanisms behind these effects are unknown. Recent studies have shown that leptin may serve as a molecular link between psoriasis and MS, suggesting that high leptin concentrations may exacerbate psoriasis. However, the molecular mechanism of this effect is still unclear. We aimed to investigate the effect of leptin on autophagy in patients with psoriasis. Methods: From January 2021 to June 2022 in PLA General Hospital, we enrolled 51 patients with psoriasis, including 21 patients with MS and 30 without MS, and 30 healthy controls who had undergone nevus surgery. We measured the epidermal leptin, P62, and Lc3B concentrations of patients by immunohistochemistry, and measured the serum leptin concentration by enzyme-linked immunosorbent assay. We then performed correlation analyses to compare these proteins' concentrations between patients with concomitant psoriasis and MS, patients with psoriasis alone, and healthy control groups. Additionally, we performed western blotting after in vitro culture of Hacat cells with different concentrations of leptin and measured the expression levels of the autophagy markers Beclin1, Lc3B, and P62; the differentiation markers K10, K16, and K17; and PI3K/AKt/mtOR signaling pathway-related proteins of Hacat cells. Next, we transfected AtG5 into Hacat cells to revert autophagy and used the specific PI3K inhibitor LY294002 to block PI3K/AKt/mtOR signaling. the expression levels of K10, K16, and K17 of Hacat cells were again measured. One-way analysis of variance was used for the comparison of means of multiple samples, and LSD-t post hoc test was used for comparison between the 2 groups. the counting data were analyzed by the chi-square test. correlations were evaluated by Pearson correlation analysis. Results: the serum l

关键词： autophagy keratinocyte differentiation leptin metabolic syndrome PI3K/AKt/mtOR psoriasis signal pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

音樂放鬆療法對改善結腸鏡檢查者疼痛的效果研究

引用

镜湖医学 2024年第1期24卷 75-77页

作者：黃家駒澳門鏡湖醫院護理部中国

目的用音樂療法作為放鬆的介入方式,以減輕結腸鏡檢查者術中的不適,促進結腸鏡檢查的進程及改善舒適度。方法2021年4月12日~5月18日,於澳門某醫院對104位結腸鏡檢查者進行研究,分為研究組及對照組(每組各52人)。研究組結腸鏡檢查者介入... 详细信息

目的用音樂療法作為放鬆的介入方式,以減輕結腸鏡檢查者術中的不適,促進結腸鏡檢查的進程及改善舒適度。方法2021年4月12日~5月18日,於澳門某醫院對104位結腸鏡檢查者進行研究,分為研究組及對照組(每組各52人)。研究組結腸鏡檢查者介入音樂療法,對照組予常規照護,比較兩組疼痛評分(numerical rating scale,NRS)、收縮壓、舒張壓、心率及結腸鏡檢查進鏡時間的差異。結果兩組研究參與者在NRS疼痛評分、術中及術後血壓及心率無差異;兩組結腸鏡檢查的進鏡時間均數沒有顯著差異;但進鏡時間≦10分鐘組(非困難腸鏡組)與>10分鐘組(困難腸鏡組)則存在組間差異,在非困難腸鏡組中,研究組進鏡時間為6.2±1.6分鐘,對照組進鏡時間為7.2±1.6分鐘(t=-2.075,P<0.05);困難腸鏡組則沒有顯著差異。結論音樂放鬆療法對結腸鏡檢查者的疼痛緩解、血壓及心率穩定沒有顯著作用,但仍可縮短“非困難腸鏡”的進鏡時間。

关键词：音樂療法結腸鏡檢查疼痛進鏡時間

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microscopic Image Analysis for Life Science Applications 1 Har/Cdr

引用

丛书名： Bioinformatics & Biomedical Imaging

2008年

作者： wong, Stephen t. c. Machiraju, Raghu Rittscher, Jens

ISBN: (数字)9781596932371

ISBN: (纸本)9781596932364;9781596932371

Bioimaging in life sciences is a burgeoning area that is of growing interest to today's professionals and researchers in the field. this is the first book that bridges the gap between biomedical imaging and the bioscience community. this unique resource gives professionals a detailed understanding of imaging platforms, fluorescence imaging, and fundamental image processing algorithms. Further, it guides readers through the application of advanced image analysis methods and techniques to specific biological problems. the book presents applications that span a wide range of scales, from the detection of signaling events in sub-cellular structures, to the automated analysis of tissue structures. Other critical areas discussed include the dynamics of cell populations and in vivo microscopy. A DVD is also included. It contains full-color images, movies and other valuable supplementary material that further illustrate topics discussed in the book

关键词： Image analysis Microscopy Imaging systems

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

ScHEDULING tWO GROUPS OF JOBS WItH INcOMPLEtE INFORMAtION

引用

Systems Science and Systems Engineering 2003年第1期12卷 73-81页

作者： GuochuanZHANG XiaoqiangcAI c.K.wong DepartmentofMathematics ZhejiangUniversityHangzhou30027P.R.China DepartmentofSystemsEngineeringandEngineeringManagement TheChineseUniversityofHongKongShatinN.T.HongKong DepartmentofcomputerScienceandEngineering TheChineseUniversityofHangKongShatinN.T.HongKong

In real world situations, most scheduling problems occur neither as complete off-line nor as complete on-line models. Most likely, a problem arises as an on-line model with some partial information. In this article, we consider such a model. We study the scheduling problem P(n1,n2), where two groups of jobs are to be scheduled. the first job group is available beforehand. As soon as all jobs in the first group are assigned, the second job group appears. the objective is to minimize the longest job completion time (makespan). We show a lower bound of 3/2 even for very special cases. Best possible algorithms are presented for a number of cases. Furthermore, a heuristic is proposed for the general case. the main contribution of this paper is to discuss the impact of the quantity of available information in designing an on-line algorithm. It is interesting to note that the absence of even a little bit information may significantly affect the performance of an algorithm.

关键词： Machine scheduling worst-case analysis on-line algorithm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synchronized off-harmonic probe laser with highly variable pulse duration for laser-plasma interaction experiments

引用

High Power Laser Science and Engineering 2024年第1期12卷 99-107页

作者： J.Hornung Y.Zobus H.Lorenté c.Brabetz B.Zielbauer V.Bagnoud GSI Helmholtzzentrum für Schwerionenforschung GmbH DarmstadtGermany Institut d’Optique UniversitéParis-SaclayPalaiseauFrance Institut für Angewandte Physik Technische Universität DarmstadtDarmstadtGermany

this paper presents the development and experimental utilization of a synchronized off-harmonic laser system designed as a probe for ultra-intense laser±plasma interaction experiments. the system exhibits a novel seed-generation design,allowing for a variable pulse duration spanning over more than three orders of magnitude, from 3.45 picoseconds to 10 nanoseconds. this makes it suitable for various plasma diagnostics and visualization techniques. In a side-view configuration, the laser was employed for interferometry and streaked shadowgraphy of a laser-induced plasma while successfully suppressing the self-emission background of the laser±plasma interaction, resulting in a signal-to-self-emission ratio of 110 for this setup. these properties enable the probe to yield valuable insights into the plasma dynamics and interactions at the PHELIX facility and to be deployed at various laser facilities due to its easy-to-implement design.

关键词： interferometry laser-plasma interaction off-harmonic probe laser streaked shadowgraphy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

conservation form of Helbing's fluid dynamic traffic flow model

引用

Applied Mathematics and Mechanics(English Edition) 2011年第9期32卷 1109-1118页

作者：李书峰张鹏 S.c.wong Shanghai Institute of Applied Mathematics and Mechanics Shanghai University Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai University Department of civil Engineering The University of Hong Kong

A standard conservation form is derived in this *** hyperbolicity of Helbing＇s fluid dynamic traffic flow model is proved,which is essential to the general analytical and numerical study of this *** the basis of this conservation form,a local discontinuous Galerkin scheme is designed to solve the resulting system *** evolution of an unstable equilibrium traffic state leading to a stable stop-and-go traveling wave is *** simulation also verifies that the model is truly improved by the introduction of the modified diffusion coefficients,and thus helps to protect vehicles from collisions and avoide the appearance of the extremely large density.

关键词： conservation form hyperbolicity local discontinuous Galerkin method stop-and-go wave

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

comparative Analysis of Machine Learning Models for customer churn Prediction in the U.S. Banking and Financial Services: Economic Impact and Industry-Specific Insights

引用

Journal of Data Analysis and Information Processing 2024年第3期12卷 388-418页

作者： Omoshola S. Owolabi Prince c. Uche Nathaniel t. Adeniken Oghenekome Efijemue Samuel Attakorah Oluwabukola G. Emi-Johnson Emmanuel Hinneh Department of Data Science Carolina University Winston-Salem NC USA Department of computer Science Austin Peay State University Clarkesville TN USA Department of Statistical Sciences Wake Forest University Winston-Salem NC USA

customer churn poses a significant challenge for the banking and finance industry in the United States, directly affecting profitability and market share. this study conducts a comprehensive comparative analysis of machine learning models for customer churn prediction, focusing on the U.S. context. the research evaluates the performance of logistic regression, random forest, and neural networks using industry-specific datasets, considering the economic impact and practical implications of the findings. the exploratory data analysis reveals unique patterns and trends in the U.S. banking and finance industry, such as the age distribution of customers and the prevalence of dormant accounts. the study incorporates macroeconomic factors to capture the potential influence of external conditions on customer churn behavior. the findings highlight the importance of leveraging advanced machine learning techniques and comprehensive customer data to develop effective churn prevention strategies in the U.S. context. By accurately predicting customer churn, financial institutions can proactively identify at-risk customers, implement targeted retention strategies, and optimize resource allocation. the study discusses the limitations and potential future improvements, serving as a roadmap for researchers and practitioners to further advance the field of customer churn prediction in the evolving landscape of the U.S. banking and finance industry.

关键词： churn Prediction Machine Learning Economic Impact Industry-Specific Insights Logistic Regression Random Forest Neural Networks

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：