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Development of a Novel Tissue-Specific Method to Detect Cytokeratin 20-Positive Circulating Tumor Cells in Metastatic Colorectal Cancer

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Advances in Modern Oncology Research 2018年第4期4卷 1-6页

作者： sze chuen cesar wong Charles Chan Ming Lok Chu So Shan Department of Health Technology and Informatics Faculty of Health and Social SciencesHong Kong Polytechnic UniversityHong Kong Special Administrative RegionChina Department of Clinical Oncology Faculty of MedicineChinese University of Hong KongHong Kong Special Administrative RegionChina

Introduction:Although many studies have shown the vast potential of circulating tumor cells(CTCs)detection in cancer diagnosis and prognosis,our understanding of their clinical significance is still far from complete.A major obstacle arises from the lack of well-established tumor or tissue-specific markers to detect CTCs by immunocytochemical staining after immunomagnetic enrichment(IE).Methods:We have established the utility of cytokeratin 20(CK20),a gastrointestinal tract specific marker,for the specific detection and identification of colorectal cancer(CRC)*** breakthrough was successfully validated in spike-in experiments using CRC cell line models followed by a pilot study which recruited 32 metastatic CRC patients,25 benign colorectal diseases patients and 27 normal ***:CK20-positive CTCs were detected in 90%metastatic CRC patients but not in benign colorectal diseases patients and normal subjects using this refined ***:These impressive results have laid the foundation for further development of CK20-positive CTCs as a promising marker in diagnosis,prognostication and treatment monitoring of metastatic CRC.

关键词： Tissue-specific cytokeratin 20-positive circulating tumor cells metastatic colorectal cancer

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Current and future molecular diagnostics in colorectal cancer and colorectal adenoma

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World Journal of Gastroenterology 2014年第14期20卷 3847-3857页

作者： Andy Hin-Fung Tsang Ka-Ho Cheng Apple Siu-Ping wong Simon Siu-Man Ng Brigette Buig-Yue Ma Charles Ming-Lok Chan Nancy Bo-Yin Tsui Lawrence Wing-Chi Chan Benjamin Yat-Ming Yung sze-chuen cesar wong Department of Health Technology and Informatics Faculty of Health and Social SciencesHong Kong Polytechnic University Department of Surgery Prince of Wales Hospital State Key Laboratory in Oncology in South China Sir Y K Pao Centre for CancerDepartment of Clinical OncologyHong Kong Cancer Institute and Prince of Wales HospitalThe Chinese University of Hong Kong

Colorectal cancer(CRC)is one of the most prevalent cancers in developed *** the other hand,CRC is also one of the most curable cancers if it is detected in early stages through regular colonoscopy or *** CRC develops slowly from precancerous lesions,early detection can reduce both the incidence and mortality of the *** occult blood test is a widely used non-invasive screening tool for *** fecal occult blood test is simple and cost-effective in screening CRC,there is room for improvement in terms of the accuracy of the *** dysregulations have been found to play an important role in CRC *** better understanding of the molecular basis of CRC,there is a growing expectation on the development of diagnostic tests based on more sensitive and specific molecular markers and those tests may provide a breakthrough to the limitations of current screening tests for *** this review,the molecular basis of CRC development,the characteristics and applications of different non-invasive molecular biomarkers,as well as the technologies available for the detection were *** review intended to provide a summary on the current and future molecular diagnostics in CRC and its pre-malignant state,colorectal adenoma.

关键词： Colorectal cancer Colorectal adenoma Molecular diagnostics Fecal occult blood test Non-invasive

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JAG2基因沉默影响大肠癌细胞株侵袭能力的研究

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中华临床医师杂志（电子版） 2015年第15期9卷 78-81页

作者：何婉黄思铨邓权通陈居杲朱莲玉夏俊贤许瑞莲申维玺刘利平暨南大学第二临床医学院肿瘤科深圳518020 香港理工大学医疗技术和咨询学系 999077 暨南大学第二临床医学院胆胰外科深圳518020

目的 Notch信号在肠上皮细胞种系分化和引发肠腺瘤和肠癌中起着关键的作用。本文试图探讨Notch信号的配体JAG2在大肠癌中的作用。方法 Real-Time PCR和Western blot分别检测JAG2特异性si RNA对JAG2 m RNA及蛋白表达的抑制率,细胞增殖实... 详细信息

目的 Notch信号在肠上皮细胞种系分化和引发肠腺瘤和肠癌中起着关键的作用。本文试图探讨Notch信号的配体JAG2在大肠癌中的作用。方法 Real-Time PCR和Western blot分别检测JAG2特异性si RNA对JAG2 m RNA及蛋白表达的抑制率,细胞增殖实验和细胞侵袭实验观察si RNA对细胞增殖及侵袭能力的影响,人类肿瘤转移RT2 Profiler?PCR Array用于筛选JAG2下游靶基因,并应用特异性抑制化合物抑制靶蛋白活性。结果特异性si RNA对JAG2 m RNA及蛋白的表达具有抑制作用(P0.05),对细胞侵袭能力明显抑制(P<0.05),而组织蛋白K(CTSK)活性降低可能参与了这一过程。结论 JAG2基因可能对大肠癌细胞的侵袭转移能力起促进作用。

关键词： RNA,小分子干扰细胞增殖 JAG2 细胞侵袭

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