检索结果-南通市图书馆

Translational lab-to-clinic hurdles in stem cell therapy

在线全文

学校读者我要写书评

暂无评论

Chinese Neurosurgical Journal 2016年第4期2卷 229-232页

作者： sydney corey Trenton Lippert Cesar V.Borlongan Center of Excellence for Aging and Brain Repair Department of Neurosurgery and Brain Repair University of South Florida Morsani College of Medicine 12901 Bruce B.Downs Blvd Tampa FL 33612 USA

Several experimental therapeutics have been demonstrated to be safe and effective in animal models of neurological disorders, but have failed when they reached the clinic. Postulated factors that affected the poor clinical outcomes include the lack of proper treatment controls and clinically relevant animal models. This failure of lab-to-clinic translation has plagued many neuroprotective drugs for stroke. With this in mind, the stem cell therapy field has cautiously approached translating stem cell products for clinical application, in that a negative clinical trial may hinder the entire regenerative medicine therapeutics. Here, we discuss the many translational challenges associated with stem cell therapy in the setting of stroke.

关键词： Physical therapy Cerebral ischemia Neurogenesis Cell transplantation

Pathological links between stroke and cardiac arrest

在线全文

学校读者我要写书评

暂无评论

Chinese Neurosurgical Journal 2017年第1期3卷 40-43页

作者： Shaila Ghanekar sydney corey Trenton Lippert Cesar V.Borlongan Center of Excellence for Aging and Brain Repair Department of Neurosurgery and Brain Repair University of South Florida Morsani College of Medicine 12901 Bruce B.Downs Blvd Tampa 33612 FL USA

There may be a pathological connection between cardiac failure and ischemic stroke. In this article we describe pertinent research that demonstrates subsequent death of cardiac and neural myocytes in the post ischemic stroke brain. Current stroke therapy overlooks the connection between cardiac and cerebrovascular events and fails to address the shared risk factors. Current pre-clinical stroke investigations have provided evidence that suggests the presence of an indirect cell death pathway in which toxic molecules emanate from the stroke brain and trigger cardiac cell death. On the other hand, other studies highlight the presence of a reverse cell death cascade in which toxic molecules from the heart, following cardiac arrest, travel to the brain and induce ischemic cell death. Further examination of these putative cell death pathways between ischemic stroke and cardiac arrest will prompt the advancement of innovative treatments specifically targeting both diseases, leading to ameliorated clinical results of patients diagnosed with heart failure and ischemic stroke.

关键词： Cerebral ischemia Heart disease Stem cells

Neuroprotective effects of human bone marrow mesenchymal stem cells against cerebral ischemia are mediated in part by an anti-apoptotic mechanism

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2019年第4期14卷 597-604页

作者： Yuyang Zhang Seongjin Yu Julian P.Tuazon Jea-Young Lee sydney corey Lauren Kvederis Chase Kingsbury Yuji Kaneko Cesar V.Borlongan Department of Neurosurgery and Brain Repair Center of Excellence for Aging and Brain Repair University of South Florida Department of Pharmacology/School of Life Science and Biopharmaceutical Science Shenyang Pharmaceutical University

Transplantation of human bone marrow mesenchymal stem cells(hMSCs) stands as a potent stroke therapy, but its exact mechanism remains unknown. This study investigated the anti-apoptotic mechanisms by which hMSCs exert neuroprotective effects on cerebral ischemia. Primary mixed cultures of rat neurons and astrocytes were cultured and exposed to oxygen-glucose deprivation. A two-hour period of "reperfusion" in standard medium and normoxic conditions was allowed and immediately followed by hMSCs and/or Bcl-2 antibody treatment. Cell viability of primary rat neurons and astrocytes was determined by 3-(4,5-dimethylthianol-2-yl)-2,5 diphenyl tetrazolium bromide and trypan blue exclusion methods. hMSC survival and differentiation were characterized by immunocytochemistry, while the concentration of Bcl-2 in the supernatant was measured by enzyme-linked immunosorbent assay to reveal the secretory anti-apoptotic function of hMSCs. Cultured hMSCs expressed embryonic-like stem cell phenotypic markers CXCR4, Oct4, SSEA4, and Nanog, as well as immature neural phenotypic marker Nestin. Primary rat neurons and astrocytes were protected from oxygen-glucose deprivation by hMSCs, which was antagonized by the Bcl-2 antibody. However, Bcl-2 levels in the supernatants did not differ between hMSCand non-treated cells exposed to oxygen-glucose deprivation. Neuroprotective effects of hMSCs against cerebral ischemia were partially mediated by the anti-apoptotic mechanisms. However, further studies are warranted to fully elucidate this pathway.

关键词： apoptosis Bcl-2 antibody human mesenchymal stem cells ischemia neuroprotection oxygen glucose deprivation

An update on stem cell therapy for neurological disorders: cell death pathways as therapeutic targets

在线全文

学校读者我要写书评

暂无评论

Chinese Neurosurgical Journal 2017年第2期3卷 98-105页

作者： sydney corey Shaila Ghanekar Jake Sokol John H.Zhang Cesar V.Borlongan Center of Excellence for Aging & Brain Repair Department of Neurosurgery and Brain Repair University of South Florida College of Medicine 12901 Bruce B.Downs Blvd Tampa Florida 33612 USA Departments of Anesthesiology Neurosurgery Neurology and Physiology Loma Linda University Medical Center 11234 Anderson St Loma Linda CA 92354 USA

Here, we compiled ten papers detailing recent strides in the field of stem cell therapy, which address critical issues relevant to pursuing this experimental treatment for clinical applications in brain disorders. Topics include efficacy, safety, and mechanism of action underlying cell therapy, emerging technologies and combination pharmacotherapies with cell transplantation directed at improving the functional outcomes, and evaluation of key translational components of advancing novel therapeutics to the clinic, including the need for vis-à-vis comparisons with the gold standard of treatment post-injury (i.e., physical rehabilitation). A bioethics paper is also incorporated here to further appreciate the current status of cell therapy in the community setting. The overall goal of this special volume is to provoke a meaningful assessment of the lessons we have learned in recent years and to use such knowledge to carefully translate safe and effective applications of cell therapy, and its mechanism of action for the treatment of neurological disorders.

关键词： Brain disorders Cell transplantation Safety Efficacy Mechanism Clinical trials Bioethics Biomaterials