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Fish Nutrition Additives in SHK-1 Cells: Protective Effects of Silymarin

Advances in Bioscience and Biotechnology 2016年第2期7卷 55-62页

作者： Rodrigo Sanchez Pamela Olivares Erico Carmona Allisson Astuya Hector herrera Jorge Parodi Laboratorio Fisiología de la Reproducción Escuela de Medicina Veterinaria Núcleo de Investigación en Producción Alimentaria Facultad de Recursos Naturales Universidad Católica de Temuco Temuco Chile Empresa Vitapro Chile Castro Región de Los Lagos Chile Grupo de Genotoxicología Escuela de Medicina Veterinaria Núcleo de Estudios Ambientales Facultad de Recursos Naturales Universidad Católica de Temuco Temuco Chile Laboratory of Cell Culture and Marine Genomics Marine Biotechnology Unit Faculty of Natural and Oceanographic Sciences University of Concepcion and Sur-Austral COPAS Program University of Concepcion Concepción Chile

In nutrition for productive species, additives play an important role in boosting physiological processes. Only in recent years studies include models of the effects on fish cells of these additives. We observed effects of silymarin, a compound highly utilized in aquaculture. The cell line SHK-1 was used derived from the upper liver of the Atlantic salmon as a biological model. Samples were exposed to silymarin in incrementing time and concentrations, to evaluate by MTT and number of cells, the effects on cell viability. Also, oxidative stress models were used to find the protector effects of silymarin against these agents. Our data indicate that a dose of 100 ppm of silymarin is sufficient to stimulate cellular proliferation. Cultures were exposed to high glucose (15 mM) or H2O2 (0.1 mM) in presence of or absence of silymarin at 100 ppm. We observed that the toxic effects of both compounds were blocked by the presence of silymarin. Our results indicate that it is important to evaluate additive effects at a cellular level. Also, silymarin does have proliferative effects, and protect against cellular injury in our models. Our study helps to generate more rational applications of additives in the industry and presents new challenges in order to better manipulate the model in the laboratory, allowing us to obtain new evidence regarding the microalgae’s biology through in vitro studies.

关键词： Fish Nutrition Silymarin Cell Cultures

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Local Changes in Chromatin Accessibility and Transcriptional Networks Underlying the Nitrate Response in Arabidopsis Roots

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Molecular Plant 2019年第12期12卷 1545-1560页

作者： Jose M.Alvarez Tomas C.Moyano Tao Zhang Diana E.Gras Francisco J.herrera Viviana Araus Jose AQ'Brien Laura Carrillo Joaquin Medina Jesus Vicente-Carbajosa Jiming Jiang Rodrigo A.Gutierrez Pontificia Universidad Catolica de Chile SantiagoChile Yangzhou University YangzhouChina Instituto de Agrobiotecnologia del Litoral CONICETSanta FeArgentina University of California BerkeleyCAUSA Trancura Biosciences Inc.San FranciscoCA 94158USA Centro de Biotecnología y Genómica de Plantas(CBGP UPM-INIA)Universidad Politecnica de Madrid(UPM)Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA)Campus de Montegancedo28223-Pozuelo de AlarcóMadridSpain Department of Horticulture University of Wisconsin-MadisonMadisonWI 53706USA Department of Plant Biology and Horticulture Michigan State UniversityMI 48824USA

Transcriptional regulation,determined by the chromatin structure and regulatory elements interacting at pro-moter regions,is a key step in plant responses to environmental ***(NO3-)is a nutrient signal that regulates the expression of hundreds of genes in Arabidopsis ***,we integrate mRNA sequencing,genome-wide RNA polymeraseⅡ(RNPⅡ),chromatin immunoprecipitation sequencing,and DNase Sequencing datasets to establish the relationship between RNPⅡoccupancy and chromatin accessibility in response to NO3-treatments in Arabidopsis *** footprinting allowed us to identify in vivo regula-tory elements controlling gene expression in response to ***3--modulated transcription factor(TF)footprints are important for a rapid increase in RNPⅡoccupancy and transcript accumulation over *** mapped key TF regulatory interactions and functionally validated the role of NAP,an NAC-domain containing TF,as a new regulatory factor in *** together,our study provides a comprehensive view of transcriptional networks in response to a nutrient signal in Arabidopsis roots.

关键词： nitrate transcriptional regulation footprinting RNA polymeraseⅡ regulatory networks

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Arsenic and chromium resistance mechanisms in the Micrococcus luteus group

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Pedosphere 2023年第4期33卷 600-611页

作者： Ivan ARROYO-herrera Brenda ROMÁN-PONCE Rafael BUSTAMANTE-BRITO Joseph GUEVARA-LUNA Erika Yanet TAPIA-GARCÍA Violeta LARIOS-SERRATO Nannan ZHANG Paulina ESTRADA-DE LOS SANTOS En Tao WANG María Soledad VÁSQUEZ-MURRIETA Escuela Nacional de Ciencias Biológicas Instituto Politécnico NacionalCiudad de México 11340(Mexico) Universidad Politécnica del Estado de Morelos Jiutepec 62550(Mexico) Centro de Ciencias Genómicas Universidad Autónoma de MéxicoCuernavaca 62210(Mexico) CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilisation&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041(China)

High arsenic(As)and chromium(Cr)concentrations are currently receiving attention because of their negative effects on the environment and human *** inhabiting contaminated environments have developed resistance mechanisms against the toxicity of these ***,members of the bacterial genus Micrococcus have been isolated from different toxic metal-contaminated environments;however,knowledge concerning its resistance mechanisms to As and Cr toxicity remains *** luteus strains(An24,Mh,NE2TL6,and NE2TTS4)were isolated from the endosphere and soil of two heavy metal-contaminated sites in Mexico to identify differences in the resistance mechanisms by the *** *** strains were resistant to As(As^(3+)and As^(5+)),chromate,dichromate,cobalt,copper,nickel,and *** analysis indicated that the heavy metal-resistant strains(An24,Mh,NE2TL6,and NE2TTS4)could be assigned to the *** group and had more heavy metal-resistant genes(transporters,chaperones,and enzymes)compared to reference strains of the *** group,*** NCTC 2665^(T)and Micrococcus endophyticus JCM 16951^(T).The resistant bacteria were able to biotransform As^(3+)and As^(5+)through a carbon source-dependent *** biotransformation of As5+was potentially carried out in the cytoplasm through a thioredoxin-dependent pathway,which may be coupled with biosorption.A qualitative analysis of organic acids(OAs)identified a change in the OA profile of the metal-resistant strains that was As-or *** genomic and phenotypic findings suggest that the four *** group strains evaluated in the current study have developed resistance mechanisms that may enable their survival in contaminated sites.

关键词： arsenophore biosorption genome analysis heavy metal-resistant strain immobilization organic acid pollutant

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Inexpensive and combined technique: Use of suction tracheal catheter and hydrogen peroxide for the evacuation of intravesical clots

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Asian Journal of Urology 2022年第1期9卷 99-100页

作者： Fred Alain Montelongo-Rodriguez Carlos Daniel Guerra-Catanon Mario Vazquez-herrera Adrian Gutierrez-Gonzalez Lauro Salvador Gomez-Guerra Urology Department Hospital Universitario“Dr.Jose Eleuterio Gonzalez”Universidad Autonoma de Nuevo LeonMonterreyMexico

Dear Editor,Persistent hematuria could trigger urinary retention,leading the patient to the emergency department for abdominal pain,decreased urinary output,anemia,and azotemia[1,2].Conventional bladder clot evacuation requires a three-way catheter with continuous irrigation and aspiration with a syringe or Ellik evacuator[3].Sometimes a large and well-organized hematoma requires an open cystostomy[1,4].We want to introduce a combined method using hydrogen peroxide as well as suction technique with an endotracheal catheter to evacuate intravesical clots.

关键词： urinary bladder catheter

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What is the prognosis of emphysematous pyelonephritis associated with extended-spectrum beta-lactamases producing microorganisms?

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Asian Journal of Urology 2022年第2期9卷 146-151页

作者： JoséIván Robles-Torres Marco Alberto Ocaña-Munguía JoséGustavo Arrambide-herrera Adrián Mauricio Martínez-Fernández Rodrigo Romero-Mata Lauro Salvador Gómez-Guerra Urology Department Hospital Universitario"Dr.Jose´Eleuterio Gonza´lez"Universidad Auto´noma de Nuevo LeonMonterreyNuevo LeonMexico

Objective:To describe the microbiological characteristics in emphysematous pyelonephritis(EPN),demonstrate the frequency of extended-spectrum beta-lactamase(ESBL)microorganisms,and determine if these microorganisms are associated with the prognosis of patients with ***:We conducted a retrospective study in patients with a diagnosis of EPN in a tertiary care hospital of the northeast region of Mexico during the period from January 2011 to January *** variables were analyzed to determine association with the presence of ESBL-producing *** significance was set with p<***:A total of 63 patients were included;55(87.3%)of them were females,with a median age of 55(interquartile range:45-65)*** management was indicated in 38.1%;42.9%were treated with ureteral stent;12.7%with open or percutaneous drainage;15.8%with early nephrectomy;and 9.5%with delayed *** mortality was 13(20.6%)cases;23(36.5%)cases required admission to the intensive care *** most frequent microorganism isolated was Escherichia coli(n=34,53.9%).ESBL microorganisms were found in 31.7%of the *** significant association of ESBL was found with admission to the intensive care unit,or with increased ***:To our knowledge,this is the first study that evaluates ESBL microorganisms as a prognostic factor in *** factors associated with a poor prognosis in patients with EPN have been *** microbiological factors,specifically ESBL-producing bacteria,do not seem to influence in the prognosis of these patients.

关键词： Emphysematous pyelonephritis Extended spectrum betalactamase Mortality Intensive care unit admission

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Life Cycle Assessment on Real Time in a Coffee Machine

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Journal of Chemistry and Chemical Engineering 2014年第12期8卷 1142-1149页

作者： Jorge Dominguez-Patino Antonio Rodriguez Martinez Rosenberg Javier Romero Israel herrera Orozco Posgraduate School in Engineering and Applied Science FCQ1-CIlCAp Autonomous University of Morelos State Cuernavaca 62209 Mexico Research Centre of Engineering and Applied Science Autonomous University of Morelos State Cuernavaca 62209 Mexico Research Centre of Energy Environment and Technology Madrid 28040 Spain

The environmental impact during the preparation of coffee beverages was evaluated on real time. The functional unit is a cup of coffee prepared from 7 g of ground coffee and 125 mL of tap water. The boundaries system considered are assembly process, electricity process, tap water process, coffee process and municipal waste process. Based on boundary system, the life cycle inventory is carbon dioxide, 50.31 g; coal, brown, 53.72 rag; coal hard, 0.9906 g; dinitrogen monoxide, 0.9575 mg; natural gas, 0.0020 m^3; methane, fossil, 13.82 mg; oil, crude, 1.012 g; uranium, 15.02 ug. The life cycle impact assessment is determined using the sum of the contributions of the impacts shown in the inventory analysis, each one multiplied by a coefficient called the ＂characterization factor＂, which indicates the scale of the potential contributed by the individual substance to the effect. The results show the advantages of using the LCA （life cycle assessment） on real time as it provides information from both quality and environmental parameters allowing taking actions based on timely information. The preparation of a cup of coffee produced an environmental load of 50.9 g of CO2 equivalents and Non Renewable energy equivalents to 151 kJ; the sugar process and materials transportation were not considered.

关键词： LCA coffee cup real time

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Improved method for XPS and ARXPS data fitting

Improved method for XPS and ARXPS data fitting

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第18届国际真空大会

作者： A. herrera-Gomez J. Muoz-Flores CINVESTAV-Unidad Queretaro Queretaro 76230Mexico Departamento de Física CINVESTAVDistrito Federal 07360Mexico

X-Ray Photoelectron Spectroscopy can provide chemical and, if acquired at different angles, depth-profile information. Data-fitting is employed to quantify the various peaks contributing to a core-level spectrum. In most cases, each peak corresponds to a particular chemical environment. When the binding energy difference among the peaks is equal or larger than the width of the peaks, the quantification is straightforward. For cases in which the binding energy is smaller, the accuracy on the determination of the area, width, and center of the peaks becomes much more dependent on the noise level of the data. For quantitative studies, it is fundamental to employ data analysis methods that are the least sensible to noise. Sequential-fitting is a common practice on ARXPS data analysis. It consist on first estimating the center and width of the peaks from the data acquired at one of the angles, and then using those parameters as a starting guess for fitting the data for the rest of the angles. An improvement of this method consists on averaging the centers and widths of the peaks obtained at the different angles, and then employing these values to assess the areas of the peaks for each angle. The full-use of the combined information contained in the data-set is optimized by the simultaneous fitting method. It consists of the assessment of the center and width of the peaks by fitting the data at all the angles simultaneously. Computer-generated data was employed to compare the sensitivity regarding to noise between the sequential, averaged-sequential, and simultaneous fitting methods. It will be shown that the latter is significantly more robust and could provide reliable results even for very noisy data. Among the examples of the application of the simultaneous fitting method that will be presented is 1) the analysis of the O 1s and N 1s XPS data from hafnium oxynitride grown on silicon oxynitride nanofilms and 2) the discrimination of the sp2 and sp3 contributions to the

关键词： Simultaneous fitting Sequential fitting, Robust method, Noise, XPS, ARXPS, Data fitting

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Seismic response of high-rise steel framed buildings with Chevron-braced designed according to Venezuelan codes

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Natural Science 2012年第8期4卷 694-698页

作者： Ronald Ugel Juan Carlos Vielma Reyes herrera Sigrit Perez Alex Barbat Senior Technical School of Roads Canals and Ports Engineers of Barcelona Technical University of Catalonia. Barcelona Spain Structural Engineering Department School of Civil Engineering Lisandro Alvarado University. Barquisimeto Venezuela

The object of this study is to determine the seismic response of regular high-rise steel buildings with chevron-braced frames. Mechanics models of three buildings of 14, 18 and 20 stories are studied, all of them with similar geometric characteristics in plant and elevation. These models are realized using prescriptions and parameters from venezuelan design codes. The seismic action is carry on through varius synthetic design spectrum compatible accelerograms defined by the seismic codes in this study, with three levels of intensity corresponding to three specific Limit States. Dynamic analysis is used to compute parameters of ductility, over strength and maximum displacements. From these results it can be concluded that chevron-braced frames presented a good overall performance and non V-braced frames show greater damage due to dynamic actions, validating non linear dynamic analysis as a very powerful tool to seismic-resistance design and chevron-braced frames as a very useful choice in improving the response of tall steel structures. since this lateral bracing system is absent from Venezuelan seismic codes.

关键词： Framed Steel Buildings Chevron Braced Frames Non Linear Analysis Over Strength Seismic Response

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Dystrophic Calcinosis in the Hands of a Patient with Rheumatoid Arthritis and Secundary Sjogren’s Syndrome

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Open Journal of Rheumatology and Autoimmune Diseases 2013年第1期3卷 56-58页

作者： Daniel Jaramillo-Arroyave Gerardo Quintana Federico Rondon-herrera Antonio Iglesias-Gamarra Internal Medicine RheumatologyDepartment of Internal MedicineRheumatology UnitUniversidad Nacional de ColombiaBogotáColombia

Salts of calcium phosphate and inorganic phosphate are normally found in serum and extracellular fluids, balancing through poorly understood factors that prevent abnormal tissue deposition of these minerals. However, in those tissues that are injured, especially due to chronic inflammatory processes, a predisposition to the deposition of these minerals is developed, triggering what has been called Dystrophic Calcinosis (DC), common in different Connective Tissue Diseases (CTD), especially dermatomyositis and scleroderma, but there is no a frecuent association with diseases like Rheumatoid Arthritis (RA) and Sj?gren Syndrome (SS). We report a case of a female patient of 63 years old with RA and Secundary SS who presents with DC in the hands and no evidence of other connective tissue.

关键词： Dystrophic Calcinosis Rheumatoid Arthritis Sjogren Syndrome

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Assessing the Crop Growing Period According to the Climate Change Forecasts for Marina Baixa (SE Spain)

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Agricultural Sciences 2015年第9期6卷 1079-1088页

作者： Mario herrera Hassane Moutahir Carlos Alberto González Esteban Chirino Juan Bellot Department of Life Sciences University State Amazonia Puyo Ecuador Department of Ecology University of Alicante Alicante Spain Multidisciplinary Institute for Environmental Studies “Ramón Margalef” University of Alicante Alicante Spain AdMobilze.Computer Vision Engineer Miami FL USA Faculty of Natural Resources Polytechnic Higher School of Chimborazo (ESPOCH) Riobamba Ecuador

The present work aims to assess the likely effects of climate change on the length of growing period (LGP) of crops in Marina Baixa (SE, Spain). LGP can be assessed by a balance between preci- pitation and reference evapotranspiration. Less rainfall and an increased evapotranspiration, forecast by Global Climate Models (GCMs), are considered as a high risk for agriculture. This area is located in a semiarid climate region where water is a very limited resource. It is a typical example of areas where the agricultural sector has to compete for water with the tourism industry. In this context, by using observed and projected precipitation data set (model HadCM3, Scenario A2), calculating reference evapotranspiration (ETo), and applying the frequency analysis of a probability-type method, we estimated the growing period length in the observed period (1961-1990) and three 30-year future periods (2011-40, 2041-70 and 2071-99) in the study area. The results show a drop in annual precipitations (-?30%) and an increased ETo (+18%) towards the end of this century with respect to the observed period (mean annual rainfall: 356 mm;mean ETo: 1476 mm). The results also show a decrease in the number of decades (10 days) when precipitation exceeds half of the ETo, which means shorter growing periods as the 21st century advances. This expected reduction in growing period length towards the end of the present century will imply that many rainfed crops, like olives, almonds and cereals, will require a higher irrigation water supply to maintain suitable growth and performance levels. The results are an early warning to manage water resources in Marina Baixa in a sustainable way.

关键词： Climate Change Growing Period Frequency Analysis Semiarid Mediterranean

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