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Identification, occurrence, and validation of DRE and ABRE Cis-regulatory motifs in the promoter regions of genes of Arabidopsis thaliana

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Journal of Integrative Plant Biology 2014年第4期56卷 388-399页

作者： Sonal Mishra Aparna Shukla swati upadhyay Sanchita Pooja Sharma Seema Singh Ujjal J.Phukan Abha Meena Feroz Khan Vineeta Tripathi Rakesh Kumar Shukla Ashok Shrama Biotechnology Division CSIR-Central Institute of Medicinal and Aromatic Plants A Laboratory Under Council of Scientific and Industrial Research Metabolic and Structural Biology Division CSIR-Central Institute of Medicinal and Aromatic Plants A Laboratory Under Council of Scientific and Industrial Research Botany Department CSIR-Central Drug Research Institute (CSIR-CDRI)

Plants posses a complex co-regulatory network which helps them to elicit a response under diverse adverse conditions. We used an in silico approach to identify the genes with both DRE and ABRE motifs in their promoter regions in Arabidopsis thaliana. Our results showed that Arabidopsis contains a set of 2,052 genes with ABRE and DRE motifs in their promoter regions. Approximately 72~o or more of the total predicted 2,052 genes had a gap distance of less than 40o bp between DRE and ABRE motifs. For positional orientation of the DRE and ABRE motifs, we found that the DR form （one in direct and the other one in reverse orientation） was more prevalent than other forms. These predicted 2,o52 genes include 155 transcription factors. Using microarray data from The Arabidopsis Information Resource （TAIR） database, we present 44 transcription factors out of 155 which are upregulated by more than twofold in response to osmotic stress and ABA treatment. Fifty-one transcripts from the one predicted above were validated using semiquantitative expression analysis to support the microarray data in TAIR. Taken together, we report a set of genes containing both DRE and ABRE motifs in their promoter regions in A. thaliana, which can be useful to understand the role of ABA under osmotic stress condition.

关键词： Abscisic acid abscisic acid-responsive elements dehydration-responsive element osmotic stress response

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Carotenoids:Extraction strategies and potential applications for valorization of under-utilized waste biomass

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Food Bioscience 2022年第4期48卷 630-645页

作者： swati Tiwari Priya Yawale Neelam upadhyay Dairy Technology Division ICAR-National Dairy Research InstituteKarnal132001HaryanaIndia

Carotenoids are organic compounds and widely distributed pigments that provide shades of yellow,orange or red to agricultural,horticultural and aqua *** not only are natural colourants but also play a vital role in both plant and human health,besides holding potential(especiallyβ-carotene)to address much prevalent vitamin A deficiency around the *** bio-wastes generated from fruits and vegetable processing industries like carrot pomace,tomato peels,etc.;fish processing industry and;non-edible sources like marigold flower are rich sources of *** sources could be potentially used for extraction of carotenoids for an array of ***,organic solvents have been used for their extraction,most of which are ***,scientists have commenced their researches on alternative green solvents for extraction purposes as these besides retaining natural colour,also provide numerous health *** oils are one such example of green solvents which are edible,safe,natural,non-toxic,and environment friendly,and help in harnessing bio-functional properties from bio-wastes and other non-edible *** review recapitulates distribution of carotenoids in these sources;existing conventional strategies and recent developments in innovative extractions;followed by potential applications of extracted carotenoids in food,pharma and cosmetic industries.

关键词： Carotenoids Carrot bio-waste Tomato peel waste Marigold flower Vegetable oil Extraction

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Stability assessment of emulsion of carotenoids extracted from carrot bio-waste in flaxseed oil and its application in food model system

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Food Bioscience 2022年第3期47卷 1016-1022页

作者： swati Tiwari Neelam upadhyay Ashish Kumar Singh Dairy Technology Division ICAR-National Dairy Research InstituteKarnal132001HaryanaIndia

The research was conducted on the stability assessment of emulsion of carotenoids extracted from carrot-pomace in omega-3 rich flaxseed oil,followed by converting this emulsion into freeze-dried powder,and its application as natural functional colorant in the preparation of flavored *** study indicated that emulsion was stable over a range of pH (5–9),temperature (forewarming to HTST pasteurization) and ionic strength (0.1–0.75 M NaCl) except at pH 4,sterilization temperature and 1.0 M NaCl based on zeta potential and particle size of emulsion analysed at these *** percent (out of 8,10 and 12%) of freeze-dried emulsion was selected for addition as a natural functional ingredient in flavored milk on the basis of organoleptic *** fat,protein,total solids,total carotenoid content and antioxidant value (in terms of ABTS and reducing power assay) of developed product were significantly higher (p < 0.05) than *** whiteness index of control was significantly higher (p < 0.05) than the developed product,while reverse was true for yellowness ***,both the products showed total color difference of *** research concludes that carotenoid-rich emulsion can be efficiently freeze-dried and used as a functional natural colorant in flavored milk for providing it better bio-functional attributes over control.

关键词： Carotenoids Carrot bio-waste Nano-emulsion Stability studies Flavored milk

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