检索结果-南通市图书馆

EWS Knockdown and Taxifolin Treatment Induced Differentiation and Removed DNA Methylation from p53 Promoter to Promote Expression of Puma and Noxa for Apoptosis in Ewing’s Sarcoma

引用

Journal of Cancer Therapy 2014年第12期5卷 1092-1113页

作者： Mohammad Motarab Hossain swapan kumar ray Department of Pathology Microbiology and Immunology University of South Carolina School of Medicine Columbia SC USA

Ewing’s sarcoma is a pediatric tumor that mainly occurs in soft tissues and bones. Malignant characteristics of Ewing’s sarcoma are correlated with expression of EWS oncogene. We achieved knockdown of EWS expression using a plasmid vector encoding EWS short hairpin RNA (shRNA) to increase anti-tumor mechanisms of taxifolin (TFL), a new flavonoid, in human Ewing’s sarcoma cells in culture and animal models. Immunofluorescence microscopy and flow cytometric analysis showed high expression of EWS in human Ewing’s sarcoma SK-N-MC and RD-ES cell lines. EWS shRNA plus TFL inhibited 80% cell viability and caused the highest decreases in EWS expression at mRNA and protein levels in both cell lines. Knockdown of EWS expression induced morphological features of differentiation. EWS shRNA plus TFL caused more alterations in molecular markers of differentiation than either agent alone. EWS shRNA plus TFL caused the highest decreases in cell migration with inhibition of survival, angiogenic and invasive factors. Knockdown of EWS expression was associated with removal of DNA methylation from p53 promoter, promoting expression of p53, Puma, and Noxa. EWS shRNA plus TFL induced the highest amounts of apoptosis with activation of extrinsic and intrinsic pathways in both cell lines in culture. EWS shRNA plus TFL also inhibited growth of Ewing’s sarcoma tumors in animal models due to inhibition of differentiation inhibitors and angiogenic and invasive factors and also induction of activation of caspase-3 for apoptosis. Collectively, knockdown of EWS expression increased various anti-tumor mechanisms of TFL in human Ewing’s sarcoma in cell culture and animal models.

关键词： Apoptosis Differentiation Ewing’s Sarcoma EWS shRNA p53 Promoter Taxifolin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Induction of Mitochondrial Pathways and Endoplasmic Reticulum Stress for Increasing Apoptosis in Ectopic and Orthotopic Neuroblastoma Xenografts

引用

Journal of Cancer Therapy 2011年第2期2卷 77-90页

作者： Surajit Karmakar Subhasree Roy Choudhury Naren Lal Banik swapan kumar ray Department of Pathology Microbiology and Immunology University of South Carolina School of Medicine Columbia South Carolina USA.

Cancers are characterized by deregulation of multiple signaling pathways and thus monotherapies are hardly effective. Neuroblastoma, which often occurs in adrenal glands, is the most common childhood malignancy. Malignant neuroblastoma resists traditional treatments and further studies are needed for effective therapeutic interventions. We evaluated synergistic efficacy of N-(4-hydroxyphenyl) retinamide (4-HPR) and genistein (GST) for induction of apoptosis in human malignant neuroblastoma SH-SY5Y and SK-N-BE2 cells in culture and activation of multiple pathways for increasing apoptosis in ectopic and orthotopic neuroblastoma xenografts in nude mice. Combination of 4-HPR and GST synergistically reduced cell viability, caused subG1 accumulation, increased caspase-3 activity for apoptosis in vitro and reduced tumor growth in vivo. Western blotting indicated that combination therapy down regulated Id2 to induce differentiation, increased pro-apoptotic Bax and decreased anti-apoptotic Bcl-2 leading to an increase in Bax:Bcl-2 ratio, increased mitochondrial Bax level, caused mitochondrial release of Smac/Diablo, down regulation of the baculovirus inhibitor-of-apoptosis repeat containing (BIRC) proteins such as BIRC-2 and BIRC-3, and activation of calpain and caspase-3 in SH-SY5Y xenografts. Accumulation of apoptosis-inducing-factor (AIF) in cytosol and increase in caspase-4 activation suggested involvement of mitochondrial pathway and endoplasmic reticulum (ER) stress, respectively, for apoptosis in SH-SY5Y xenografts. In situ immunofluorescent labelings of SH-SY5Y and SK-N-BE2 xenograft sections showed overexpression of calpain, caspase-12, and caspase-3, and AIF, suggesting induction of mitochondrial caspase-dependent and caspase-independent pathways for apoptosis. Collectively, synergistic effects of 4-HPR and GST induced mitochondrial pathways and also ER stress for increasing apoptosis in ectopic and orthotopic neuroblastoma xenografts in nude mice.

关键词： Apoptosis Endoplasmic Reticulum Stress Genistein N-(4-Hydroxyphenyl) Retinamide Neuroblastoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Influence of scattering of SH-waves in dynamic interaction of shear wall with soil layers

引用

Earthquake Engineering and Engineering Vibration 2020年第3期19卷 583-595页

作者： Shashi kumar swapan kumar Chakraborty Department of Mathematics Sarala Birla UniversityRanchi 835103India Department of Applied Mathematics Cambridge Institute of TechnologyRanchi 835103India Department of Mathematics Birla Institute of TechnologyMesraRanchi 835215India

The dynamic soil-structure interaction of a shear wall embedded in elastic isotropic and homogeneous soil layers underlain by bedrock,subjected to SH waves,is modeled in the present *** soil layers consist of irregular interfaces and it has been shown that the scattering due to the roughness of the layers has significant effect on the displacement of both the foundation and the shear *** demonstrate the phenomena indirect boundary element method(IBEM)has been used on the basis of its validation in previous problems of similar *** system response is compared with the analytical solution of the same type of model for vertically propagating incident SH *** is observed that for the low frequency of wave,displacement is abruptly high,and as a result the combination of shear wall and foundation perceives *** thickness of the soil layer,mass of the shear wall,stiffness of the bedrock and the soil layers all affects the system frequency and displacement.

关键词： soil dynamic interaction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Modulation of autophagy for neuroprotection and functional recovery in traumatic spinal cord injury

引用

Neural Regeneration Research 2020年第9期15卷 1601-1612页

作者： swapan K.ray Department of Pathology Microbiology and Immunology University of South Carolina School of Medicine

Spinal cord injury(SCI) is a serious central nervous system trauma that leads to loss of motor and sensory functions in the SCI patients. One of the cell death mechanisms is autophagy, which is ‘self-eating' of the damaged and misfolded proteins and nucleic acids, damaged mitochondria, and other impaired organelles for recycling of cellular building blocks. Autophagy is different from all other cell death mechanisms in one important aspect that it gives the cells an opportunity to survive or demise depending on the circumstances. Autophagy is a therapeutic target for alleviation of pathogenesis in traumatic SCI. However, functions of autophagy in traumatic SCI remain controversial. Spatial and temporal patterns of activation of autophagy after traumatic SCI have been reported to be contradictory. Formation of autophagosomes following therapeutic activation or inhibition of autophagy flux is ambiguous in traumatic SCI studies. Both beneficial and harmful outcomes due to enhancement autophagy have been reported in traumatic SCI studies in preclinical models. Only further studies will make it clear whether therapeutic activation or inhibition of autophagy is beneficial in overall outcomes in preclinical models of traumatic SCI. Therapeutic enhancement of autophagy flux may digest the damaged components of the central nervous system cells for recycling and thereby facilitating functional recovery. Many studies demonstrated activation of autophagy flux and inhibition of apoptosis for neuroprotective effects in traumatic SCI. Therapeutic induction of autophagy in traumatic SCI promotes axonal regeneration, supporting another beneficial role of autophagy in traumatic SCI. In contrast, some other studies demonstrated that disruption of autophagy flux in traumatic SCI strongly correlated with neuronal death at remote location and impaired functional recovery. This article describes our current understanding of roles of autophagy in acute and chronic traumatic SCI, crosstalk

关键词： apoptosis autophagy autophagy modulation neuroprotection traumatic spinal cord injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of molasses binder on the physical and mechanical properties

引用

International Journal of Minerals,Metallurgy and Materials 2019年第1期26卷 41-51页

作者： Anand Babu Kotta Anshuman Patra Mithilesh kumar swapan kumar Karak Department of Metallurgical and Materials Engineering National Institute of Technology

Molasses was used as an alternative binder to the bentonite binder. The change in moisture absorption by pellets prepared with different iron ores and different molasses contents were investigated. Iron ore properties exerted the major effect on pellet behavior and final pellet quality. The absorbed moisture content of pellets prepared without binder, bentonite-added pellets, and molasses-added pellets were in the range of 7.72%–9.95%, 9.62%–10.84%, and 6.14%-6.69%, respectively. The wet pellet compressive strength of molasses-added pellets(43–230 N/pellet) was superior to that of bentonite-added pellets(9.47–11.92 N/pellet). The compressive strength of dried molasses-modified pellets increased to 222–394 N/pellet, which is currently the highest value achieved for dried pellets.

关键词： iron ore molasses binder moisture absorption pellet strength physical and mechanical properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neuroprotective role of Noggin in spinal cord injury

引用

Neural Regeneration Research 2023年第3期18卷 492-496页

作者： Nadia Al-Sammarraie Mohammed Mahmood swapan K.ray Department of Pathology Microbiologyand ImmunologyUniversity of South Carolina School of MedicineColumbiaSCUSA

Spinal cord injury is one of the leading causes of morbidity and mortality among young adults in many countries including the United *** in the regeneration of neurons is one of the main obstacles that leave spinal cord injury patients with permanent paralysis in most *** research has found that preventing acute and subacute secondary cellular damages to the neurons and supporting glial cells can help slow the progression of spinal cord injury pathogenesis,in part by reactivating endogenous regenerative proteins including Noggin that are normally present during spinal cord *** is a complex protein and natural inhibitor of the multifunctional bone morphogenetic proteins,and its expression is high during spinal cord development and after induction of spinal cord *** this review article,we first discuss the change in expression of Noggin during pathogenesis in spinal cord ***,we discuss the current research knowledge about the neuroprotective role of Noggin in preclinical models of spinal cord ***,we explain the gap in the knowledge for the use of Noggin in the treatment of spinal cord *** results from extensive in vitro and in vivo research have revealed that the therapeutic efficacy of Noggin treatment remains debatable due to its neuroprotective effects observed only in early phases of spinal cord injury but little to no effect on altering pathogenesis and functional recovery observed in the chronic phase of spinal cord ***,clinical information regarding the role of Noggin in the alleviation of progression of pathogenesis,its therapeutic efficacy,bioavailability,and safety in human spinal cord injury is still lacking and therefore needs further investigation.

关键词： apoptosis astrocyte differentiation axon myelination axon regeneration bone morphogenetic protein glial scar heterotrophic ossification neurogenesis neuropathic pain Noggin spinal cord injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Potential therapeutic roles of retinoids for prevention of neuroinflammation and neurodegeneration in Alzheimer’s disease

引用

Neural Regeneration Research 2019年第11期14卷 1880-1892页

作者： Bhaskar C. Das Somsankar Dasgupta swapan K. ray Department of Medicine Icahn School of Medicine at Mount SinaiNew YorkNYUSA Department of Neuroscience and Regenerative Medicine Institute of Molecular Medicine and GeneticsAugusta UniversityAugustaGAUSA Department of Pathology Microbiologyand ImmunologyUniversity of South Carolina School of MedicineColumbiaSCUSA

All retinoids, which can be natural and synthetic, are chemically related to vitamin A. Both natural and synthetic retinoids use specific nuclear receptors such as retinoic acid receptors and retinoid X receptors to activate specific signaling pathways in the cells. Retinoic acid signaling is extremely important in the central nervous system. Impairment of retinoic acid signaling pathways causes severe pathological processes in the central nervous system, especially in the adult brain. Retinoids have major roles in neural patterning, differentiation, axon outgrowth in normal development, and function of the brain. Impaired retinoic acid signaling results in neuroinflammation, oxidative stress, mitochondrial malfunction, and neurodegeneration leading to progressive Alzheimer’s disease, which is pathologically characterized by extra-neuronal accumulation of amyloid plaques(aggregated amyloid-beta) and intra-neurofibrillary tangles(hyperphosphorylated tau protein) in the temporal lobe of the brain. Alzheimer’s disease is the most common cause of dementia and loss of memory in old adults. Inactive cholinergic neurotransmission is responsible for cognitive deficits in Alzheimer’s disease patients. Deficiency or deprivation of retinoic acid in mice is associated with loss of spatial learning and memory. Retinoids inhibit expression of chemokines and neuroinflammatory cytokines in microglia and astrocytes, which are activated in Alzheimer’s disease. Stimulation of retinoic acid receptors and retinoid X receptors slows down accumulation of amyloids, reduces neurodegeneration, and thereby prevents pathogenesis of Alzheimer’s disease in mice. In this review, we described chemistry and biochemistry of some natural and synthetic retinoids and potentials of retinoids for prevention of neuroinflammation and neurodegeneration in Alzheimer’s disease.

关键词： Alzheimer's disease amyloid plaques neurofibrillary tangles neuroinflammation neurodegeneration retinoids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Study of Improved Load Sharing Methodologies for Distributed Generation Units Connected in a Microgrid

引用

CSEE Journal of Power and Energy Systems 2017年第3期3卷 311-320页

作者： Rajashree Dhua Debashis Chatterjee swapan kumar Goswami Electrical Engineering Department Jadavpur UniversityKolkata 700032India

In this paper,an improved load sharing strategy is proposed for distributed generation units(DGs)connected in a *** frequency and voltage droop control result in unacceptable active and reactive power *** proposed method formulates a suitable algorithm for load sharing in the islanded *** feeder power loss and the line impedance voltage drops are minimized so as to regulate the voltage at the point of common coupling(PCC)at its nominal *** desired DG output voltages are calculated and a linear relationship is obtained between the shared active and reactive powers and the DG output voltages.A master DG controller sets the frequency which is followed by other DG *** reference powers for the DG units are adjusted so as to maintain the rated PCC *** proposed strategy is verified taking into account the DG ratings,unequal line impedance drops,feeder losses,change in system impedance and effect of DG local loads and formulates an improved power sharing strategy that also facilitates PCC voltage regulation under variable loading *** and experimental results are presented to verify the effectiveness of the proposed method.

关键词： DG local loads distributed generation(DG) line impedance drops microgrid point of common coupling(PCC)voltage regulation power sharing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Future directions for using estrogen receptor agonists in the treatment of acute and chronic spinal cord injury

引用

Neural Regeneration Research 2016年第9期11卷 1418-1419页

作者： swapan K. ray Supriti Samntaray Naren L. Banik Department of Pathology Microbiology and Immunology University of South Carolina School of Medicine Columbia SC USA Department of Neurosciences Medical University of South Carolina Charleston SC USA Department of Neurology and Neurosurgery Medical University of South Carolina Charleston SC USA Ralph H. Johnson Veterans Administration Medical Center Charleston SC USA

All synthetic and natural estrogen receptor agonists, in- cluding the most potent physiological molecule estrogen or estradiol （E2）, work typically via activation of nuclear estrogen receptor alpha （ERα） and estrogen receptor beta （ERβ）. Both ERα and ERβ modulate the expression of a variety of genes in the cells. Neurons and glial cells express ERa and ERβ. Many studies so far from our and other laboratories have firmly established the mode of actions that ERα and ERβ agonists are very promising anti-inflammatory and neuroprotective agents in the treatment of neurodegenera- rive diseases and injuries including spinal cord injury （SCI）（Chakrabarti et al., 2014a）.

关键词： Future directions for using estrogen receptor agonists in the treatment of acute and chronic spinal cord injury SCI

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Detrital and authigenic(?) baddeleyite(ZrO_2) in ferromanganese nodules of Central Indian Ocean Basin

引用

Geoscience Frontiers 2011年第4期2卷 571-576页

作者： Bibhuranjan Nayak swapan kumar Das Kalyan kumar Bhattacharyya CSIR National Metallurgical Laboratory Jamshedpur 831007India

Occurrence of baddeleyite（ZrO_2） which is a rare mineral has been recorded in ferromanganese nodules of Central Indian Ocean Basin（CIOB）.The mineral occurs either as independent isolated sub-rounded to elliptical grains or in clusters forming fine subhedral crystals（〈3μm） within ferromanganese concretionary growth *** mode of occurrence,texturai features and chemistry of the mineral suggest detrital and possibly an authigenic origin for *** authigenic origin it is proposed that zirconium might have got released either from the terrigenous sediments or the altered seafloor rocks forming halogen complexes and subsequently it has re-precipitated in me form of baddeleyite within manganese nodules under oxic to sub-oxic conditions.

关键词： Manganese nodule Baddeleyite Zirconium mobility Authigenic Indian Ocean

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：