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Age Estimation by Quantitative Cytomorphometric Study Using Buccal Mucosal Cells and Panoramic Radiography: A Comparative Forensic Study

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Journal of Forensic Science and Medicine 2023年第3期9卷 219-227页

作者： Kavya Keerthika Nutulapati Sri Veda Donupati M P V Prabhat G Sarat swagathika mishra Department of Oral Medicine and Radiology Dr.Sudha and Nageswara Rao Siddhartha Institute of Dental SciencesGannavaramAndhra PradeshIndia

Aim: This study aims to estimate the age of an individual using buccal cells by calculating the average cell size and comparing with pulp/tooth area ratio on mandibular canine and linear measurements related to mental foramen using panoramic radiography (PR). Materials and Methods: One hundred healthy individuals undergoing PR for various dental procedures were included in this study after obtaining consent. Pulp/tooth ratio using Cameriere et al. method on mandibular canine and linear measurements related to mental foramen, i.e., the distance between the superior and inferior border of the mandible (S-I) and the mandible’s superior border to the superior margin of the mental foramen (S-sM) were estimated using Adobe Photoshop image editing software. Later, buccal smears obtained from the same individual were fixed with 95% alcohol and stained with Papanicolaou’s stain for cytomorphometric analysis. The age was calculated using the regression analysis and correlated with chronological age. Results: Statistical significance and better correlation were obtained pulp/ tooth area ratio, the distance of S-I of all age groups. However, the distance of S-sM proved to be more effective in Group 4 (51-60 years) and buccal cell size was found to be comparable to the chronological age particularly in Group 5 (above 60 years). Conclusion: In the present study, the accuracy of estimated age compared to the chronological age was more promising for pulp/tooth area ratio, followed by S-I, S-sM, and cell size.

关键词： Age estimation cell size cytomorphometric analysis mental foramen pulp/tooth area ratio

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