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circ_BACH2通过调控miR-370-3p影响甲状腺乳头状癌的恶性生物学行为

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中华肿瘤杂志 2024年第7期46卷 663-675页

作者：孙璞冯勇周连仲裴斐苏玢乔晓丞天津市第四中心医院耳鼻咽喉头颈外科天津300140 上海理工大学附属市东医院耳鼻咽喉科上海200438

目的探讨circ_BACH2对甲状腺乳头状癌恶性生物学行为的影响及分子机制。方法收集天津市第四中心医院2017—2019年51例甲状腺乳头状癌患者的癌组织和癌旁正常组织。实时荧光定量PCR法检测癌组织、癌旁正常组织和细胞中circ_BACH2、miR-37... 详细信息

目的探讨circ_BACH2对甲状腺乳头状癌恶性生物学行为的影响及分子机制。方法收集天津市第四中心医院2017—2019年51例甲状腺乳头状癌患者的癌组织和癌旁正常组织。实时荧光定量PCR法检测癌组织、癌旁正常组织和细胞中circ_BACH2、miR-370-3p和GIT1 mRNA的表达水平,流式细胞术检测细胞凋亡情况和细胞周期变化,平板克隆形成实验检测细胞克隆形成数,细胞计数试剂盒8检测细胞增殖能力,Transwell检测细胞迁移和侵袭能力,Western blot法检测蛋白表达,双荧光素酶报告实验检测circ_BACH2、miR-370-3p和GIT1之间的靶向关系,裸鼠成瘤实验检测抑制circ_BACH2的表达对小鼠体内肿瘤的影响。结果甲状腺乳头状癌组织中circ_BACH2、GIT1 mRNA和蛋白表达水平高于癌旁正常组织,miR-370-3p表达水平低于癌旁正常组织,甲状腺癌细胞TPC-1、sw579中circ_BACH2、GIT1 mRNA和蛋白表达水高于人甲状腺正常细胞Nthy-ori3-1,miR-370-3p表达水平低于Nthy-ori3-1细胞(均Psw579细胞G0/G1期细胞比例高于si-NC组,S期细胞比例、细胞吸光度值低于si-NC组,细胞克隆形成数[分别为(43±5)个和(54±8)个]低于si-NC组[分别为(100±6)个和(100±9)个],细胞凋亡率[分别为(19.60±2.40)%和(18.10±2.10)%]高于si-NC组[分别为(4.30±0.20)%和(5.10±0.23)%],细胞迁移数[分别为(61±7)个和(58±6)个]、细胞侵袭数[分别为(45±6)个和(47±4)个]均低于si-NC组[分别为(134±15)个、(112±11)个、(113±11)个和(92±9)个,均Pe-cadherin表达水平高于si-NC组(均P<0.001)。抑制miR-370-3p的表达逆转敲减circ_BACH2对甲状腺乳头状癌细胞增殖、迁移、侵袭和凋亡的影响,过表达GIT1逆转过表达miR-370-3p对甲状腺癌细胞增殖、迁移、侵袭和凋亡的影响。注射稳定转染sh-circ_BACH2的TPC-1细胞的裸鼠在培养35 d后肿瘤体积缩小[(535±91)mm^(3)和(857±114)mm^(3)],肿瘤重量减少[(0.62±0.13)mg和(1.06±0.15)mg,均P<0.05]。裸鼠肿瘤组织中circ_BACH2、GIT1 mRNA表达水平降低,miR-370-3p mRNA表达水平升高(均P<0.05)。结论抑制circ_BACH2可能通过靶向调控miR-370-3p/GIT1从而抑制体外甲状腺乳头状癌细胞的增殖、迁移、侵袭,促进细胞凋亡,并抑制体内肿瘤的生长。

关键词：甲状腺肿瘤 circ_BACH2 miR-370-3p GIT1

PAD4介导H3cit促结肠癌细胞增殖、侵袭和上皮间质转化的机制研究

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局解手术学杂志 2024年第4期33卷 281-287页

作者：王永琦刘丽杰何冬雷陈勇海南医学院第一附属医院胃肠肿瘤外科海南海口570102

目的探究肽基精氨酸脱亚胺酶4(PAD4)介导的瓜氨酸化组蛋白H3(H3cit)在结肠癌细胞增殖、侵袭和上皮间质转化中的作用及其相关机制。方法收集我院30例结肠癌患者的血清和30例健康受试者的血清,eLISA检测血清中PAD4和H3cit水平,并分析两... 详细信息

目的探究肽基精氨酸脱亚胺酶4(PAD4)介导的瓜氨酸化组蛋白H3(H3cit)在结肠癌细胞增殖、侵袭和上皮间质转化中的作用及其相关机制。方法收集我院30例结肠癌患者的血清和30例健康受试者的血清,eLISA检测血清中PAD4和H3cit水平,并分析两者相关性。将oe-NC、过表达PAD4(oe-PAD4)和sh-NC、sh-NLRP3载体转染至人结肠癌sw480细胞中,并设为oe-NC组、oe-PAD4组、oe-PAD4+sh-NC组、oe-PAD4+sh-NLRP3组。采用qRT-PCR检测PAD4、NLRP3、IL-1β、IL-18基因表达水平;Western blot检测PAD4、H3cit、NLRP3、e-cadherin、N-cadherin蛋白表达;CCK-8检测细胞增殖水平;Transwell检测细胞侵袭水平;ChIP-qPCR检测H3cit在NLRP3转录起始位点(TSS)的富集水平。结果与健康受试者比较,结肠癌患者血清中PAD4和H3cit水平均升高,且两者呈正相关(Pe-NC组比较,oe-PAD4组PAD4、NLRP3、IL-1β、IL-18基因表达水平升高,PAD4、H3cit、NLRP3、N-cadherin蛋白表达升高,e-cadherin蛋白表达降低,细胞增殖、侵袭水平升高,H3cit在NLRP3 TSS区富集增加(Pe-PAD4+sh-NC组比较,oe-PAD4+sh-NLRP3组NLRP3、IL-1β、IL-18基因表达水平降低,NLRP3和N-cadherin蛋白表达降低,e-cadherin蛋白表达升高,细胞增殖、侵袭水平降低(P<0.05)。结论结肠癌患者体内PAD4和H3cit水平升高,PAD4介导的H3cit通过转录调控NLRP3的表达,促进结肠癌细胞增殖、侵袭和上皮间质转化。

关键词：结肠癌肽基精氨酸脱亚胺酶4 瓜氨酸化组蛋白H3 上皮间质转化转录调控

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浙江苍南近岸海域溢油数值模拟及对附近生态敏感区的影响

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浙江水利科技 2024年第4期52卷 1-9页

作者：樊稳州徐海刘勇浙江省水利河口研究院(浙江省海洋规划设计研究院) 浙江杭州310017

利用MIKe21 HD模块建立浙江苍南近岸海上风电场工程水域水动力模型,并用实测潮位潮流数据对模型进行验证,结果表明模型能较好模拟研究水域的水动力特征;在此基础上,根据欧拉-拉格朗日“油粒子”理论,采用MIKe21 SA模块建立工程水域溢油... 详细信息

利用MIKe21 HD模块建立浙江苍南近岸海上风电场工程水域水动力模型,并用实测潮位潮流数据对模型进行验证,结果表明模型能较好模拟研究水域的水动力特征;在此基础上,根据欧拉-拉格朗日“油粒子”理论,采用MIKe21 SA模块建立工程水域溢油漂移扩散模型,模拟不同工况条件下油膜的漂移路径和扫海面积,分析溢油事故对周边生态敏感区的影响。结果表明:夏季主导风sw风向条件下,油膜向外海漂移,未对周边生态敏感区产生影响;冬季主导风NNe风向条件下,油膜对工程水域以南七星岛海洋保护区产生影响;不利风S风向条件下,油膜对工程水域以北南麂列岛海洋保护区产生影响;不利风e风向条件下,油膜对苍南近岸渔业产卵场产生影响。工程建设应制定溢油事故应急处理预案,以防溢油事故对周边生态敏感区产生不利影响。

关键词：海上风电场溢油 MIKe21 油膜数值模拟

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神威超级计算机运行时故障定位方法

计算机研究与发展 2024年第1期61卷 86-97页

作者：高剑刚郑岩于康彭达佳李宏亮刘勇何王全陈德训王飞国家并行计算机工程技术研究中心北京100190 江南计算技术研究所江苏无锡214083

随着高性能计算机的性能不断提升、系统规模不断提高,系统和应用的错误率也不可避免地持续增多.快速发现和定位系统及应用级的错误、为用户提供高质量服务,成为了超级计算机系统设计开发过程中急需考虑的问题.超级计算机系统中硬件故障... 详细信息

随着高性能计算机的性能不断提升、系统规模不断提高,系统和应用的错误率也不可避免地持续增多.快速发现和定位系统及应用级的错误、为用户提供高质量服务,成为了超级计算机系统设计开发过程中急需考虑的问题.超级计算机系统中硬件故障与异常、软件程序的错误等都会导致用户大规模并行应用的错误、挂死与退出.如何快速准确定位错误现场,让管理员或用户以此为基础查看异常发生的故障进行高精度、高效率的诊断,是维护高性能计算系统可靠性的重要基础.高性能计算机传统的故障定位主要通过硬件异常跟踪、系统日志分析和程序主动探测等方法,缺乏对无日志信息、无明显故障现象的程序挂死问题的定位手段,并且技术的扩展性也面临挑战.针对“新一代神威超级计算机”体系结构和sw26010-Pro众核处理器特点,提出一种运行时故障定位方法,包括基于消息传递的故障关联分析、基于全局聚合信息的在线综合分析诊断、面向申威众核处理器的异常线程过滤方法等关键技术,阐述了如何有效检测、收集、处理大量系统资源和并行进程的异常信息问题,为应对未来超大规模高性能计算中故障高效定位难题提供有效支撑.

关键词： e级计算机可靠性错误定位运行时众核处理器

Numerical Study of Dam-Break Induced Tsunami-Like Bore with A Hump of Different Slopes

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China Ocean engineering 2017年第6期31卷 683-692页

作者： CHeNG Du ZHAO Xi-zeng ZHANG Da-ke CHeN yong Ocean College Zhejiang University Zhoushan 316021 China

Numerical simulation of dam-break wave, as an imitation of tsunami hydraulic bore, with a hump of different slopes is performed in this paper using an in-house code, named a Constrained Interpolation Profile （CIP）-based model. The model is built on a Cartesian grid system with the Navier Stokes equations using a CIP method for the flow solver, and employs an immersed boundary method （IBM） for the treatment of solid body boundary. A more accurate interface capturing scheme, the Tangent of hyperbola for interface capturing/Slope weighting （THINC/sw） scheme, is adopted as the interface capturing method. Then, the CIP-based model is applied to simulate the dam break flow problem in a bumpy channel. Considerable attention is paid to the spilling type reflected bore, the following spilling type wave breaking, free surface profiles and water level variations over time. Computations are compared with available experimental data and other numerical results quantitatively and qualitatively. Further investigation is conducted to analyze the influence of variable slopes on the flow features of the tsunami-like bore.

关键词： dam break tsunami-like bore CIP method VOF method immersed boundary method

消退素D1对结肠癌细胞K-Ras/Notch信号通路串话的影响

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中国医药导报 2024年第7期21卷 18-22页

作者：杜恒吴安定余洁王飞周勇湖北省黄冈市中心医院胃肠外科湖北黄冈438000

目的探讨消退素D1(Rv D1)对sw620结肠癌细胞K-Ras/Notch信号通路串话的影响及作用机制。方法采用CCK-8和克隆形成方法评估Rv D1(0.0、62.5、125.0、250.0、500 nmol/L)对sw620结肠癌细胞短期和长期增殖的影响;将sw620结肠癌细胞分成... 详细信息

目的探讨消退素D1(Rv D1)对sw620结肠癌细胞K-Ras/Notch信号通路串话的影响及作用机制。方法采用CCK-8和克隆形成方法评估Rv D1(0.0、62.5、125.0、250.0、500 nmol/L)对sw620结肠癌细胞短期和长期增殖的影响;将sw620结肠癌细胞分成空白组、Rv D1组、K-Ras组、K-Ras+Rv D1组。空白组不进行处理,K-Ras组和K-Ras+Rv D1组转染K-Ras质粒,Rv D1组和K-Ras+Rv D1组用250 nmol/L的Rv D1处理。蛋白质印迹法检测IL-6、K-Ras、NICD、p-p65、p65、vimentin、N-cadherin和e-cadherin蛋白表达;免疫荧光法检测IL-6、K-Ras和NICD蛋白表达;Transwell实验检测细胞侵袭和迁移水平。结果 125.0、250.0、500.0 nmol/L Rv D1抑制sw620细胞增殖和克隆形成能力(Pentin、N-cadherin蛋白表达均低于空白组,e-cadherin表达高于空白组,差异有统计学意义(Pentin、N-cadherin的蛋白表达高于空白组,e-cadherin表达低于空白组,差异有统计学意义(Pentin、N-cadherin表达及细胞侵袭和迁移水平均低于K-Ras组,e-cadherin表达高于K-Ras组,差异有统计学意义(P<0.05)。结论 Rv D1通过抑制IL-6表达,抑制K-Ras对Notch信号通路串话,降低下游核因子-κB水平和上皮-间质转化特性,削弱结肠癌细胞的侵袭转移能力。

关键词：消退素D1 结肠癌细胞串话 Ras Notch

Tectonically-controlled evolution of the Late Cenozoic Nihewan Basin, North China Craton: Constraints from Stratigraphy, Mineralogy, and Geochemistry

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Acta Geologica Sinica(english edition) 2018年第2期92卷 769-785页

作者： LIU Jin CHeN Xingqiang CHI Zhenqing WANG yong MIN Longrui LI Tingdong School of Civil and Architecture engineering Xi'an Technological University Institute of Geology Chinese Academy of Geological Sciences China Railway First Survey and Design Institute Group CO. Ltd. Institute of Geomechanics Chinese Academy of Geological Sciences

The Late Cenozoic basins in the Weihe-Shanxi Graben, North China Craton are delineated by northeast-striking faults. The faults have, since a long time, been related to the progressive uplift and northeastward expansion of the Tibetan Plateau. To show the relation between the basins and faults, two Pliocene-Pleistocene stratigraphic sections （Chengqiang and Hongyanangou） in the southern part of the Nihewan Basin at the northernmost parts of the graben are studied herein. Based on the sedimentary sequences and facies, the sections are divided into three evolutionary stages, such as alluvial fan-eolian red clay, fan delta, and fluvial, with boundaries at -2.8 and -1.8 Ma. Paleocurrent indicators, the composition of coarse clastics, heavy minerals, and the geochemistry of moderate-fine clastics are used to establish the temporal and spatial variations in the source areas. Based on features from the middle- northern basin, we infer that the Nihewan Basin comprises an old Ne-sw.elongate geotectogene and a young NW-Se elongate subgeotectogene. The main geotectogene in the mid-north is a half-graben bounded by northeast-striking and northwest-dipping normal faults （e.g., Liulengshan Fault）. This group of faults was mainly affected by the Pliocene （before -2.8-2.6 Ma） NW-Se extension and controlled the deposition of sediments. In contrast, the subgeotectogene in the south was affected by northwest-striking normal faults （e.g., Huliuhe Fault） that were controlled by the subsequent weak Ne- sw.extension in the Pleistocene. The remarkable change in the sedimentary facies and provenance since -1.8 Ma is possibly a signal of either weak or strong Ne-sw.extension. This result implies that the main tectonic transition ages of -2.8-2.6 Ma and -1.8 Ma in the Weihe-Shanxi Graben are affected by the Tibetan Plateau in Pliocene-Pleistocene.

关键词： sedimentation extension Neotectonic Weihe-Shanxi Graben Tibetan Plateau

Amplification effect of near-field ground motion around deep tunnels based on finite fracturing seismic source model

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Journal of Rock Mechanics and Geotechnical engineering 2022年第6期14卷 1761-1781页

作者： Qiankuan Wang Shili Qiu Yao Cheng Shaojun Li Ping Li yong Huang Shirui Zhang State Key Laboratory of Geomechanics and Geotechnical engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China Faculty of engineering China University of GeosciencesWuhan430074China University of Chinese Academy of Sciences Beijing100049China South-Central University for Nationalities Wuhan430074China China Railway First Survey and Design Institute Group Ltd. Xi’an710000China

Dynamic failure of rock masses around deep tunnels,such as fault-slip rockburst and seismic-induced collapse,can pose a significant threat to tunnel construction *** of the most significant factors that control the accuracy of its risk assessment is the estimation of the ground motion around a tunnel caused by seismicity *** general,the characteristic parameters of ground motion are estimated in terms of empirical scaling ***,these scaling laws make it difficult to accurately estimate the near-field ground motion parameters because the roles of control factors,such as tunnel geometry,damage zone distribution,and seismic source parameters,are not *** this,the finite fracturing seismic source model(FFSSM)proposed in this study is used to simulate the near-field ground motion characteristics around deep ***,the amplification effects of ground motion caused by the interaction between seismic waves and deep tunnels and corresponding control factors are *** control effects of four factors on the near-field ground motion amplification effect are analyzed,including the main seismic source wavelength,tunnel span,tunnel shape,and range of damage *** empirical formula for the maximum amplification factor(a_(m))of the near-field ground motion around deep tunnels is proposed,which consists of four control factors,*** wavelength control factor(F_(λ)),tunnel span factor(F_(D)),tunnel shape factor(F_(s))and excavation damage factor(F_(d)).This empirical formula provides an easy approach for accurately estimating the ground motion parameters in seismicityprone regimes and the rock support design of deep tunnels under dynamic loads.

关键词： Near-field ground motion Amplification effect Seismic waves Deep tunnel Rockburst

experimental and numerical study of regular waves past a submerged breakwater

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Journal of Hydrodynamics 2019年第4期31卷 641-653页

作者： Bi-jin Liu Du Cheng Zhao-chen Sun Xi-zeng Zhao yong Chen Wei-dong Lin State Key Laboratory o f Coastal and Offshore engineering Dalian University of Technology Dalian 116024 China Ocean College Zhejiang University Zhoushan 316021 China School of Civil engineering and Architecture Xiamen University o f Technology Xiamen 361024 China

Laboratory experiments are performed to investigate the hydrodynamics around a submerged breakwater due to regular incident waves. With an in-house code, a constrained interpolation profile (CIP)-based model is employed to simulate this process. The model is built on a Cartesian grid system with the Navier-Stokes equations using a CIP method for the flow solver and an immersed boundary method (IBM) is used for the treatment of the solid body boundary. A more accurate interface capturing scheme, the tangent of hyperbola for interface capturing/slope weighting (THINC/sw) scheme, is used to track the free surface. The numerical results are compared with experimental data. Reasonably good agreement is achieved in terms of the wave profiles at six measuring stations, the flow velocities at three different space locations and the pressures of eight points on the surface of the submerged breakwater. Moreover, the water mass transfer over the breakwater is discussed using a two-phase VOF model and the wave spectrum is also presented for analysis. It is indicated that the present model can accurately predict the hydrodynamic characteristics of the wave over a submerged bar. Furthermore, the experimental data in the present work can provide reliable basic data, including the wave transformations, the velocities and the dynamic pressures, for the validation of other CFD models.

关键词： Submerged breakwater CIP method wave transform VOF method immersed boundary method