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Neural Regeneration Research 2024年第8期19卷 1647-1648页

作者： sven marcel stefan Muhammad Rafehi Drug Development and Chemical Biology Lübeck Institute of Experimental Dermatology(LIED)University of Lübeck and University Medical Center Schleswig-HolsteinLübeckGermany Department of Pathology Section of NeuropathologyTranslational Neurodegeneration Research and Neuropathology LabUniversity of Oslo and Oslo University HospitalOsloNorway School of Medical Sciences Faculty of Medicine and HealthUniversity of SydneySydneyNSWAustralia Institute of Clinical Pharmacology University Medical Center GöttingenGöttingenGermany Department of Medical Education Augsburg University MedicineAugsburgGermany

Introductory comments:The identification and validation of disease-modifying proteins are fundamental aspects in drug ***,the m ultifactority of n eurodegen era tive diseases poses a real challenge for targeted ***,the behavior of individually(over-)expressed to rget proteins in vitro is likely to differ from their actual functional behavior when embedded in cascades and pathways in vivo.

关键词： translation beyond likely

Rbfox1 controls alternative splicing of focal adhesion genes in cardiac muscle cells

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Journal of Molecular Cell Biology 2024年第1期16卷 54-65页

作者： Peter Zorn Jaime Calvo Sánchez Tala Alakhras Barbara Schreier Michael Gekle stefan Hüttelmaier marcel Köhn Junior Group‘Non-coding RNAs and RBPs in Human Diseases’ Medical FacultyUniversity of Halle–Wittenberg06120 Halle(Saale)Germany Julius Bernstein Institute of Physiology Medical FacultyUniversity of Halle–Wittenberg06112 Halle(Saale)Germany Institute of Molecular Medicine Medical FacultyUniversity of Halle–Wittenberg06120 Halle(Saale)Germany

Alternative splicing is one of the major cellular processes that determine the tissue-specific expression of protein ***,it remains challenging to identify physiologically relevant and tissue-selective proteins that are generated by alternative ***,we investigated the target spectrum of the splicing factor Rbfox1 in the cardiac muscle context in more *** using a combination of in silico target prediction and in-cell validation,we identified several focal adhesion proteins as alternative splicing targets of *** focused on the alternative splicing patterns of vinculin(metavinculin isoform)and paxillin(extended paxillin isoform)and identified both as potential Rbfox1 *** analyses suggested that both isoforms are promoted by Rbfox1 due to binding in the *** adhesions play an important role in the cardiac muscle context,since they mainly influence cell shape,cytoskeletal organization,and cell–matrix *** data confirmed that depletion of Rbfox1 changed cardiomyoblast morphology,cytoskeletal organization,and multinuclearity after differentiation,which might be due to changes in alternative splicing of focal adhesion ***,our results indicate that Rbfox1 promotes alternative splicing of focal adhesion genes in cardiac muscle cells,which might contribute to heart disease progression,where downregulation of Rbfox1 is frequently observed.

关键词： Rbfox1 alternative splicing focal adhesion cardiac muscle cells

Deformable microlaser force sensing

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Light(Science & Applications) 2024年第6期13卷 1196-1209页

作者： Eleni Dalaka Joseph SHill Jonathan H.H.Booth Anna Popczyk stefan R.Pulver Malte C.Gather marcel Schubert Centre of Biophotonics SUPASchool of Physics and AstronomyUniversity of St AndrewsNorth HaughSt AndrewsUK Humboldt Centre for Nano-and Biophotonics Department of ChemistryUniversity of CologneKölnGermany School of Psychology and Neuroscience University of St AndrewsSt Mary’s QuadSouth StreetSt AndrewsUK Institute for Bioengineering of Catalonia BarcelonaSpain

Mechanical forces are key regulators of cellular behavior and function,affecting many fundamental biological processes such as cell migration,embryogenesis,immunological responses,and pathological *** force sensors and imaging techniques have been developed to quantify these otherwise invisible forces in single cells and in ***,current techniques rely heavily on high-resolution microscopy and do not allow interrogation of optically dense tissue,reducing their application to 2D cell cultures and highly transparent biological ***,we introduce DEFORM,deformable microlaser force sensing,a spectroscopic technique that detects sub-nanonewton forces with unprecedented spatio-temporal *** is based on the spectral analysis of laser emission from dye-doped oil microdroplets and uses the force-induced lifting of laser mode degeneracy in these droplets to detect nanometer *** validation by atomic force microscopy and development of a model that links changes in laser spectrum to applied force,DEFORM is used to measure forces in 3D and at depths of hundreds of microns within tumor spheroids and late-stage Drosophila *** furthermore show continuous force sensing with single-cell spatial and millisecond temporal resolution,thus paving the way for non-invasive studies of biomechanical forces in advanced stages of embryogenesis,tissue remodeling,and tumor invasion.

关键词： invasion otherwise hundreds

POLIII-derived non-coding RNAs acting as scaffolds and decoys

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Journal of Molecular Cell Biology 2019年第10期11卷 880-885页

作者： HendrikTauber stefan Huttelmaier marcel Kohn Institute of Molecular Medicine Section for Molecular Cell BiologyFaculty of MedicineMartin Luther University Halle-WittenbergCharles Tan ford Protein CentreKurt-Mothes-Str.3a06120 HalleGermany Julius Bernstein Institute of Physiology Faculty of MedicineMartin Luther University Halle-WittenbergCharles Tan ford Protein CentreKurt-Mothes-Str.3a06120 HalleGermany

A large variety of eukaryotic small structured POLIII-derived non-coding RNAs(ncRNAs)have been described in the ***,for only few,e.g.7SL and H1/MRP families,cellular functions are well *** the vast majority of these transcripts,cellular functions remain *** findings on the role ofY RNAs and other POLIII-derived ncRNAs suggest an evolutionarily conserved function of these ncRNAs in the assembly and function of ribonucleoprotein complexes(RNPs).These RNPs provide cellular‘machineries’,which are essential for guiding the fate and function of a variety of *** this review,we summarize current knowledge on the role of POLIII-derived ncRNAs in the assembly and function of *** propose that these ncRNAs serve as scaffolding factors that‘chaperone’RNA-binding proteins(RBPs)to form functional *** addition or associated with this role,some small ncRNAs act as molecular decoys impairing the RBP-guided control of RNA fate by competing with other RNA *** suggests that POLIII-derived ncRNAs serve essential and conserved roles in the assembly of larger RNPs and thus the control of gene expression by indirectly guiding the fate of mRNAs and IncRNAs.

关键词： ncRNA POLIII RBP scaffold RBP decoy

Blood-brain barrier modeling: challenges and perspectives

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Neural Regeneration Research 2015年第6期10卷 889-891页

作者： Tobias Ruck stefan Bittner sven G.Meuth Department of Neurology University of Münster

The blood-brain barrier （BBB） forms a highly selective inter- face between blood and brain. Extensive research efforts have completely changed our view of the BBB in the last years, from a static, impermeable barrier to a dynamic, highly regulated and specific cellular system. The BBB ultrastructurally consists of specialized brain microvascular endothelial cells （BMEC） characterized by low pinocytic activity,

关键词： BBB cell Blood-brain barrier modeling challenges and perspectives ECM

Ethernet based time synchronization for Raspberry Pi network improving network model verification for distributed active turbulent flow control

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Control Theory and Technology 2015年第2期13卷 204-210页

作者： marcel DUECK Mario SCHLOESSER stefan van WAASEN Michael SCHIEK Central Institute of Engineering Electronics and Analytics ZEA-2: Electronic SystemsForschungszentrum Juelich Gmb H ZEA-2 Wilhelm-Johnen-Straβe University of Duisburg-Essen Faculty of Engineering

Friction drag primarily determines the total drag of transport systems. A promising approach to reduce drag at high Reynolds numbers(> 104) are active transversal surface waves in combination with passive methods like a riblet surface. For the application in transportation systems with large surfaces such as airplanes, ships or trains, a large scale distributed real-time actuator and sensor network is required. This network is responsible for providing connections between a global flow control and distributed actuators and sensors. For the development of this network we established at first a small scale network model based on Simulink and True Time. To determine timescales for network events on different package sizes we set up a Raspberry Pi based testbed as a physical representation of our first model. These timescales are reduced to time differences between the deterministic network events to verify the behavior of our model. Experimental results were improved by synchronizing the testbed with sufficient precision. With this approach we assure a link between the large scale model and the later constructed microcontroller based real-time actuator and sensor network for distributed active turbulent flow control.

关键词： Raspberry Pi True Time distributed actuator and sensor network network model

Ballistic tests on hot-rolled Ti-6Al-4V plates:Experiments and numerical approaches

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Defence Technology（防务技术） 2023年第8期26卷 39-53页

作者： Alexander Janda Benjamin James Ralph Yael Demarty marcel Sorger stefan Ebenbauer Aude Prestl Ingo Siller Martin Stockinger Helmut Clemens Department of Materials Science Montanuniversitat LeobenFranz Josef-Straße 188700 LeobenAustria Department Product Engineering Montanuniversitat LeobenFranz Josef-Straße 188700 LeobenAustria French-German Research Institute of Saint-Louis 5 Rue du General Cassagnou68300 Saint-LouisFrance voestalpine BOEHLER Bleche GmbH&Co KG Boehler-Gasse 18680MürzzuschlagAustria

Superior ballistic performance and the lightweight character of Ti alloys are considered as main reasons for their use in armour applications against a broad spectrum of ballistic threats,***,fragment or blast *** dynamic loading caused by typical penetrators is characterized by high strain rates,only specific test methods allow a closer investigation of the respective material *** the present study,quasi-static and dynamic compression tests as well as ballistic tests were conducted on a twophase a+βalloy Ti-6Al-4V(in m%)manufactured by ***-deformation heat treatments,influencing microstructure and mechanical properties were applied in order to compare three different microstructural configurations:as-rolled,mill-annealed and ***,on the one hand,ballistic tests were employed for the determination of the ballistic limit velocity v_(50),compression tests,on the other hand,delivered essential input parameters for the application of the Johnson-Cook constitutive model in a finite element simulation of the impact *** comparison of experimental results to simulation results was supplemented by means of microstructural characterization of tested samples with the focus set on the prevalently observed deformation and damage mechanisms,as for example adiabatic shearing.

关键词： Ti-6Al-4V Ballistic performance Split Hopkinson pressure bar FE simulation Adiabatic shear bands Intermetallic phase

Toward commercial realization of quantum dot based white light-emitting diodes for general illumination

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Photonics Research 2017年第2期5卷 1-6页

作者： KEN TSHIMIZU marcel BOHMER DANIEL ESTRADA SUMIT GANGWAL stefan GRABOWSKI HELMUT BECHTEL EDWARD KANG KENNETH JVAMPOLA DANIELLE CHAMBERLIN OLEG BSHCHEKIN JYOTI BHARDWAJ Lumileds LLC—370 West Trimble RoadSan JoseCalifornia 95131USA Lumileds Netherlands B.V. Beemdstraat 425652 AB EindhovenThe Netherlands Lumileds GmbHLumileds Development Center AachenPhilipsstrasse 852068 AachenGermany

We demonstrate the first commercial production–ready white light-emitting diodes(LEDs)for the general illumination market with red colloidal quantum dots(QDs)applied in an on–chip *** show the red QDs with tunable peak emission and narrow full width at half-maximum in combination with a conventional phosphor material can lead to LED conversion efficiency improvements of 5% to 15% over commercial phosphor based LEDs at correlated color temperatures(CCTs)ranging from 5000 to 2700 ***,the challenges associated with reliability under high temperature,high blue flux intensity,and high humidity operation have been overcome to meet consumer market ***,a demonstrator lamp at 3000 K color temperature and 90 color rendering index(CRI)with QD based LEDs show a larger efficiency gain up to 17%,attributed to the reduced blue LED droop from the lower drive current and the lower heat sink temperature when compared to a standard phosphor based LED lamp output.

关键词： LEDs QDs

Evaluation of 1031 primary titanium nitride coated mobile bearing total knee arthroplasties in an orthopedic clinic

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World Journal of Orthopedics 2017年第12期8卷 922-928页

作者： stefan J M Breugem Jeroen Linnartz Inger Sierevelt Jon D Bruijn marcel J M Driessen Orthopedium Orthopedic Clinic Slotervaart Hospital

AIM To evaluate the influence of the titanium nitride(TiN) coating on the results of a total knee arthroplasty(TKA). METHODS A total of 910 patients(338 men; 572 woman), with a mean age of 65(range 36-94) undergoing 1031 primary TKAs were assessed. Clinical evaluation and patientreported outcomes were gathered one year after surgery. The questionnaires included the Knee injury and Osteoarthritis Outcome Score(KOOS)-Dutch version, Visual Analogue Scale(VAS) pain scores in rest and during active knee movement, VAS-satisfaction scores, and EQ-5 D-3 L health scores. This was aimed to assess the overall knee function and patient satisfaction, and to enable us to make a gross comparison to other *** At a mean follow-up of 46 mo(range 1-92) the overall implant survival was 97.7% and 95.1% for any operative reason related to the implant. Twenty-three knees(2.2%) required revision surgery. Arthrofibrosis was the most common indication for a re-operation. The clinical evaluation and patient-reported outcomes revealed good to excellent patient satisfaction and function of the arthroplasty. The median postoperative VAS-pain scores on a scale of 0-100, at one year after surgery were 1 in rest and 2 during *** The Ti N coated, mobile bearing TKA results are excellent and similar to those of other widely used TKA designs. Residual pain of the knee remains a concern and the Ti N coating in combination with the mobile bearing does not seem to be the simple solution to this problem. Future research will have to show that the coating gives a better survival than the cobalt chrome version.

关键词： Total knee arthroplasty Titanium nitride coating Mobile bearing Pain Satisfaction and survival

Ribavirin in Acute Hepatitis E Infection in Patients with Gynecological Cancer:A Case Series

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Journal of Clinical and Translational Hepatology 2018年第2期6卷 237-240页

作者： Dominik Bettinger stefan Schlabe sven Pischke Michael R.Mallmann Mignon-Denise Keyver-Paik Walther Kuhn Christian P.Strassburg Robert Thimme Ulrich Spengler Department of Medicine II Medical Center University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburgGermany Berta-Ottenstein-Programme Faculty of MedicineUniversity of FreiburgFreiburgGermany Department of Internal Medicine I University of BonnBonnGermany Center for Integrated Oncology Cologne/Bonn BonnGermany Department of Medicine University Medical Center Hamburg-EppendorfHamburgGermany Department of Obstetrics and Gynecology University of BonnBonnGermany Department of Obstetrics and Gynecology University of CologneCologneGermany

Hepatitis E virus infection is usually a self-limited ***,during the last years there has been growing evidence for prolonged and chronic infection occurring in patients with *** patients with malig-nant and rheumatic diseases have been identified to be at risk for chronic hepatitis ***,their course and prognosis are not well characterized and there have been no reports of hepatitis E virus infection in patients with gynecological ***,we report three Caucasian females with breast and ovarian cancers presenting with elevation of amino-transferase levels during anticancer *** only few or no clinical hints suggested hepatitis E virus infection,the diagnosis of hepatitis E virus infection was confirmed by seroconversion,which might occur with some delay,and/or by polymerase chain *** two patients had a self-limited course,the third patient with a high-risk oncological constellation required ribavirin in order to resume *** cases highlight the need for hepatitis E virus testing in patients with gynecological cancer and elevated aminotransferase ***,these cases show that in selected high-risk patients,ribavirin treatment may be nec-essary based on the decision of a multidisciplinary team.

关键词： Hepatitis E Gynecological cancer Liver function test Chemotherapy Immunosuppression