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Genetic architecture of maize yield traits dissected by QTL mapping and GWAS in maize

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The Crop Journal 2022年第2期10卷 436-446页

作者： Xiao zhang Zhiyong Ren Bowen Luo Haixu Zhong Peng Ma Hongkai zhang Hongmei Hu Yikai Wang Haiying zhang Dan Liu Ling Wu Zhi Nie Yonghui Zhu Wenzhu He suzhi zhang Shunzong Su Yaou Shen Shibin Gao Maize Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of AgricultureChengdu 611130SichuanChina Guangxi Qingqing Agricultural Technology CO. LTD.Nanning 530000GuangxiChina Biotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066SichuanChina Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066SichuanChina State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Chengdu 611130SichuanChina g College of Agronomy Sichuan Agricultural UniversityChengdu 611130SichuanChina

The study of yield traits can reveal the genetic architecture of grain yield for improving maize production.In this study, an association panel comprising 362 inbred lines and a recombinant inbred line population derived from X178 × 9782 were used to identify candidate genes for nine yield traits. High-priority overlap(HPO) genes, which are genes prioritized in a genome-wide association study(GWAS), were investigated using coexpression networks. The GWAS identified 51 environmentally stable SNPs in two environments and 36 pleiotropic SNPs, including three SNPs with both attributes. Seven hotspots containing 41 trait-associated SNPs were identified on six chromosomes by permutation. Pyramiding of superior alleles showed a highly positive effect on all traits, and the phenotypic values of ear diameter and ear weight consistently corresponded with the number of superior alleles in tropical and temperate germplasm. A total of 61 HPO genes were detected after trait-associated SNPs were combined with the coexpression networks. Linkage mapping identified 16 environmentally stable and 16 pleiotropic QTL.Seven SNPs that were located in QTL intervals were assigned as consensus SNPs for the yield traits.Among the candidate genes predicted by our study, some genes were confirmed to function in seed development. The gene Zm00001 d016656 encoding a serine/threonine protein kinase was associated with five different traits across multiple environments. Some genes were uniquely expressed in specific tissues and at certain stages of seed development. These findings will provide genetic information and resources for molecular breeding of maize grain yield.

关键词： Maize Yield traits Genome-wide association study(GWAS) Quantitative trait locus(QTL) Coexpression networks

Research on Fuzzy Matching Query Algorithm Based on Spatial Multi-keyword

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国际计算机前沿大会会议论文集 2017年第1期 31-32页

作者： suzhi zhang Yanan Zhao Rui Yang School of Computer and Communication Engineering Zhengzhou University of Light IndustryZhengzhou 450002HenanChina

With the rapid growth of spatial data,POI(Point of Interest)is becoming ever more intensive,and the text description of each spatial point is also gradually increasing.The traditional query method can only address the problem that the text description is less and single keyword query.In view of this situation,the paper proposes an approximate matching algorithm to support spatial multi-keyword.The fuzzy matching algorithm is integrated into this algorithm,which not only supports multiple POI queries,but also supports fault tolerance of the query keywords.The simulation results demonstrate that the proposed algorithm can improve the accuracy and efficiency of query.

关键词： Spatial data Multi-keyword search Approximate query algorithm RB-tree

Characterization of a new mutant allele of the Arabidopsis Flowering Locus D (FLD) gene that controls theflowering time by repressing FLC

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Chinese Science Bulletin 2005年第23期50卷 2701-2706页

作者： CHEN Ruiqiang zhang suzhi SUN Shulan CHANG Jianhong ZUO Jianru Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate School Chinese Acadamy of Sciences Beijing 100049 China

Flowering in higher plants is controlled by both the internal and environmental cues. In Arabidopsis, several major genetic loci have been defined as the key switches to control flowering. The Flowering Locus C (FLC) gene has been shown in the autonomous pathway to inhibit the vege-tative-to-reproductive transition. FLC appears to be re-pressed by Flowering Locus D (FLD), which encodes a com-ponent of the histone deacetylase complex. Here we report the identification and characterization of a new mutant allele fld-5. Genetic analysis indicates that fld-5 (in the Was-silewskija background) is allelic to the previously character-ized fld-3 and fld-4 (in the Colombia-0 background). Genetic and molecular analyses reveal that fld-5 carries a frame-shift mutation, resulting in a premature termination of the FLD open reading frame. The FLC expression is remarkably in-creased in fld-5, which presumably attributes to the ex-tremely delayed flowering phenotype of the mutant.

关键词：阿布属开花基因位点突变等位基因植物遗传学开花时间控制

IDL Compiler API Design, Application and Validation Based on XML API

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Wuhan University Journal of Natural Sciences 2008年第1期13卷 27-32页

作者： NIE Nan LU Yansheng zhang suzhi XIE Xiaodong College of Computer Science and Technology Huazhong University of Science and Technology Wuhan 430074 Hubei China School of Computer and Communication Engineering Zhengzhou University of Light Industry Zhengzhou 450002 Henan China

In order to adapt different languages and platforms, the paper discusses how to process and validate IDL symbol table and intermediate code by XML API. It puts emphasis on IDL AP1 extension towards DOM API based on the idea of combining XML with IDL compilers. At last, the IDL compiler designing framework based on XML AP! is given, in which compiler front end can be managed and validated by some XML techniques and tools, IDL API can be validated on the basis of test, so IDL intermediate code is provided with maintainability, portability and generation. IDL compiler can be developed and extended by XML-based API, which realizes versatility and portability of modern compiler.

关键词： IDL compiler XML API UML

Cytokinin signal transduction:Known simplicity and unknown complexity

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Chinese Science Bulletin 2003年第13期48卷 1309-1315页

作者： ZHENG Binglian, SUN Jiaqiang, zhang suzhi, DENG Yan & ZUO Jianru Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China Correspondence should be addressed to Zuo Jianru (e-mail: jrzuo@ ***) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Cytokinin plays a critical role in plant growth and development by regulating cell divisions and cell differentiation. Recent studies suggest that cytokinin signaling is presumably mediated by a two-component system analogous to those found in bacteria and fungi, which transduces an external signal via a phosphorelay from the plasma membrane-anchored receptors to downstream effectors and regulators. Moreover, cytokinin signaling is highly interactive with other pathways, and many components of the pathway appear to be functionally redundant. Proper address of these questions will be crucial for our further understanding on this important network.

关键词：细胞分裂素信号转换植物生长基因突变

上海“文化东进”背景下的本土设计团队实践——以上海博物馆东馆新建工程为例

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建筑实践 2022年第5期5卷 30-37页

作者：张溯之肖蕴峰同济大学建筑设计研究院(集团)有限公司若本建筑工作室

上海的文化设施建设经过几十年的发展,现已颇见成效,无论是数量还是规模,在全国范围内都处于领先行列。“十三五”时期,上海实施“跨江东进、越河北上”战略,即向黄浦江以东、苏州河以北,聚焦功能、提升能级,建成一批具有国际水准的文... 详细信息

上海的文化设施建设经过几十年的发展,现已颇见成效,无论是数量还是规模,在全国范围内都处于领先行列。“十三五”时期,上海实施“跨江东进、越河北上”战略,即向黄浦江以东、苏州河以北,聚焦功能、提升能级,建成一批具有国际水准的文化设施,逐步形成“一轴双心、沿江沿河、一环多圈”的城市文化空间发展新格局。在此背景下,作为上海博物馆本馆的拓展和延伸,上海博物馆东馆新建工程的建设提上日程。

关键词：上海博物馆城市文化空间国际水准新建工程东馆本土设计发展新格局苏州河

植物激素配比对大蒜茎盘愈伤组织再生体系的影响

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种子 2006年第6期25卷 38-40页

作者：张素芝李纪蓉四川农业大学玉米研究所雅安625014 山东农业大学园艺学院泰安271018

植物激素对大蒜茎盘愈伤组织再生的各个环节都发挥着重要的作用。试验结果表明:2,4-D对茎盘愈伤组织的诱导具有主要作用,高浓度的2,4-D有利于大蒜愈伤组织的诱导,但并不利于其增殖分化。MS+NAA 2 mg/L+BA 1 mg/L培养基适宜于愈伤组织的... 详细信息

植物激素对大蒜茎盘愈伤组织再生的各个环节都发挥着重要的作用。试验结果表明:2,4-D对茎盘愈伤组织的诱导具有主要作用,高浓度的2,4-D有利于大蒜愈伤组织的诱导,但并不利于其增殖分化。MS+NAA 2 mg/L+BA 1 mg/L培养基适宜于愈伤组织的继代增殖,MS+NAA 1 mg/L+BA 5 mg/L培养基适宜不定芽的分化,只添加NAA或无激素的MS培养基有利于不定芽生根。

关键词：大蒜愈伤组织不定芽茎盘继代培养

玉米钼辅助因子硫酸化酶基因启动子的克隆与功能验证

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遗传 2014年第6期36卷 584-591页

作者：高学焕付凤玲牟巍周树峰张素芝李晚忱四川农业大学玉米研究所成都611130

为克服组成型启动子启动外源基因过量表达引起的诸多问题,同源克隆(Mo-molybdopterin cofactor sulfurase)基因(ABA3)的启动子(ABA3s)序列,并用PlantCARE软件分析其非生物逆境应答元件,实时定量PCR检测ABA3基因在非生物逆境诱导下的差... 详细信息

为克服组成型启动子启动外源基因过量表达引起的诸多问题,同源克隆(Mo-molybdopterin cofactor sulfurase)基因(ABA3)的启动子(ABA3s)序列,并用PlantCARE软件分析其非生物逆境应答元件,实时定量PCR检测ABA3基因在非生物逆境诱导下的差异表达后。然后,用该启动子构建启动GUS(β-glucuronidase)基因的表达载体,基因枪法转化玉米愈伤组织。经组织化学染色法检测其表达后,在高渗、高盐、低温胁迫处理及ABA诱导下检测GUS酶荧光值与荧光素酶(内参)发光值的比值(GUS/LUC),以此评价ABA3s启动子在非生物逆境胁迫下的启动活性。结果表明,ABA3基因在模拟干旱、低温、高温、高盐胁迫及ABA、乙稀诱导下差异表达,说明该基因的启动子(ABA3s)具有非生物逆境诱导活性。序列分析表明,ABA3s启动子全长777 bp,含有ARE、HSE、MBS、TGA、Circadian等多种非生物逆境胁迫应答元件。用ABA3s启动GUS基因构建的表达载体转化的玉米愈伤组织,响应干旱、低温、高温、高盐胁迫等多种非生物逆境胁迫,及ABA和乙稀诱导,GUS检测呈阳性。在8%甘露醇高渗条件下,GUS/LUC比值比空白对照高6倍。上述结果表明,ABA3s启动子具有非生物逆境诱导特性,经进一步验证其功能后,可用于玉米抗逆转基因研究。

关键词：玉米非生物胁迫钼辅助因子硫酸化酶启动子

基于MPB-Tree索引的空间数据多关键词模糊查询算法研究

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华中师范大学学报（自然科学版） 2017年第6期51卷 765-771页

作者：张素智赵亚楠杨芮郑州轻工业学院计算机与通信工程学院郑州450002

随着具有定位功能的智能设备的大量使用,产生出海量的空间数据,每条数据中包含的信息越来越多,而以往的查询算法多数仅对单个关键词进行查询,已难以满足用户更为个性化的需求.为此,本文提出一种多空间关键词模糊查询算法,在该算法中,将... 详细信息

随着具有定位功能的智能设备的大量使用,产生出海量的空间数据,每条数据中包含的信息越来越多,而以往的查询算法多数仅对单个关键词进行查询,已难以满足用户更为个性化的需求.为此,本文提出一种多空间关键词模糊查询算法,在该算法中,将以往的两维空间距离计算转化为莫顿码匹配提升查询效率,且与模糊查询算法融合支持查询的容错.实验结果表明,该算法的效率及准确性较以往查询算法有较大提高.

关键词：空间数据多关键词查询莫顿码模糊查询