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Spontaneous immortalization of human dermal microvascular endothelial cells

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World Journal of Stem Cells 2010年第5期2卷 114-120页

作者： laura DeBusk suzanne Fernandez Douglas W Strand Simon W Hayward P Charles lin Department of Radiation Oncology Vanderbilt University Medical Center Department of Urologic Surgery Vanderbilt University Medical Center Center for Cancer Research National Cancer Institute

AIM:To establish and characterize a spontaneously immortalized human dermal microvascular endothelial cell line,***:We developed a spontaneous immortalization *** approach is based on the application of optimized culture media and culture conditions without addition of any exogenous oncogenes or *** this approach,we have successfully established a microvascular endothelial cell line,iHDME1,from primary human dermal microvascular endothelial ***1 cells have been maintained in culture dishes for more than 50 passages over a period of 6 *** a GFP expressing retrovirus,we generated a GFP-stable cell line(iHDME1-GFP).RESUlTS:iHDME1 retain endothelial morphology and uniformly express endothelial markers such as VEGF receptor 2 and VE-cadherin but not α-smooth muscle actin(α-SM-actin) and cytokeratin 18,markers for smooth muscle cells and epithelial cells *** cells retain endothelial properties,migrate in response to VEGF stimulation and form 3-D vascular structures in Matrigel,similar to the parental *** is no signif icant difference in cell cycle prof ile between the parental cells and iHDME1 *** analysis indicates enhanced stemness in iHDME1 cells compared to parental ***1 cells display elevated expression of CD133 and ***:iHDME1 cells will be a valuable source for studying angiogenesis.

关键词： Spontaneous immortalization Angiogenesis Endothelial cell

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改进铜/镍冰铜的处理方法

中国有色冶金 1985年第11期 23-24页

作者： E.l.Frueh P.D.parker 张焕臻

前言美国爱麦克斯(AMAX)贱金属研究和开发公司研制出一种铜/镍冰铜的处理方法,这种方法与其它方法相比能源消耗低。在芬兰奥托昆普公司的哈贾伐尔塔冶炼厂和挪威鹰桥镍公司的克里斯蒂安松精炼厂处理铜/镍冰铜的现行实践是采用电积法从... 详细信息

前言美国爱麦克斯(AMAX)贱金属研究和开发公司研制出一种铜/镍冰铜的处理方法,这种方法与其它方法相比能源消耗低。在芬兰奥托昆普公司的哈贾伐尔塔冶炼厂和挪威鹰桥镍公司的克里斯蒂安松精炼厂处理铜/镍冰铜的现行实践是采用电积法从铜富液中提取铜。

关键词：冰铜双金属电解液电解槽预浸精密合金电解池精炼(冶金) 电解精炼阳极泥电积法焙砂

下方玻璃体腔注射后眼内炎高发病率研究（英文）

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国际眼科杂志 2015年第5期15卷 750-754页

作者： Gowtham Jonna Daniel B. Roth Howard F. Fine H. Matthew Wheatley Jonathan l. Prenner Arvin Kheterpal suzanne Cohen William J. Feuer 罗格斯大学Robert Wood Johnson医学院眼科美国新泽西州08901 迈阿密大学Miller医学院、Bascom Palmer眼科研究所美国佛罗里达州

目的:探讨下方玻璃体腔注射比上方注射出现注射后眼内炎的几率是否更高。下方小梁切除术滤过泡眼内炎的发病率高于上方小梁切除术滤过泡,有可能是因为细菌聚集在下方的泪湖。方法:经过广泛实践过的眼内炎病例数据库验证,发现在2a的研究... 详细信息

目的:探讨下方玻璃体腔注射比上方注射出现注射后眼内炎的几率是否更高。下方小梁切除术滤过泡眼内炎的发病率高于上方小梁切除术滤过泡,有可能是因为细菌聚集在下方的泪湖。方法:经过广泛实践过的眼内炎病例数据库验证,发现在2a的研究期间内,有5例眼内炎病例。同时,为了评估注射部位对发病率的影响,对治疗过的909例1 121眼共计8672次注射进行了回顾性调查。结果:5眼出现感染性眼内炎,80%的眼内炎病例均是下方注射,尽管所选病例中84.6%是位于上方注射。与感染有关的下方注射部位的危险比是(OR)22.1(P=0.006)。结论:玻璃体腔注射后感染眼内炎的几率很小,仅为0.025%。避免在偏下象限进行玻璃体腔注射可能会进一步减小眼内炎的发病率。

关键词：玻璃体内注射部位抗血管生长因子疗法眼内炎

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集硒与非集硒植物中硒、硫元素间相互作用的研究

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土壤学进展 1995年第2期23卷 26-32,56页

作者： PaulF.Bell D.R.parker A.l.Page 王美珠

比较了溶液中 SeO_4、SO_4及它们之间相互作用对非集硒植物(苜蓿)和集硒植物(***)吸收硒和硫的影响,测定了在较长生长周期(21天以上)内这两类植物对化学特性相似的两类阴离子吸收的差异.结果表明,虽然 SO_4对植物吸收 SeO_4有竞争作用,... 详细信息

比较了溶液中 SeO_4、SO_4及它们之间相互作用对非集硒植物(苜蓿)和集硒植物(***)吸收硒和硫的影响,测定了在较长生长周期(21天以上)内这两类植物对化学特性相似的两类阴离子吸收的差异.结果表明,虽然 SO_4对植物吸收 SeO_4有竞争作用,但原生集硒植物具有优先吸 Se(与吸 S 相比)能力,甚至生长在高 SO_4土壤中,这类植物含 Se 量也很高.因此,原生集硒植物在生物改良盐化的硒污染土壤与沉积物方面很有潜力.

关键词：集硒植物植物硒硫相互作用吸收

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大陆莫霍面是地壳与地幔的分界面?

宝石和宝石学杂志(中英文) 1988年第1期 38-39页

作者： W.l.Griffin suzanne Y.0'Reilly 彭少梅

高压捕虏体的研究表明,在绝大多数大陆区的下面,镁铁质岩石是下地壳的主要组成部分,在地幔的最上部也很丰富.根据捕虏体资料可以建立地热和地层剖面,为真实地解释地球物理资料提供岩性条件和物理参数.在高热流大陆区,据地震反射资料确... 详细信息

高压捕虏体的研究表明,在绝大多数大陆区的下面,镁铁质岩石是下地壳的主要组成部分,在地幔的最上部也很丰富.根据捕虏体资料可以建立地热和地层剖面,为真实地解释地球物理资料提供岩性条件和物理参数.在高热流大陆区,据地震反射资料确定的莫霍面可能比地壳与地幔的分界面更深.克拉解剖面中"热"下地壳-上地幔剖面所记录到的地震资料的差异,可以仅用温度变化来解释,不必认为岩性有巨大差异.

关键词：下地壳大陆区镁铁质岩石莫霍面地幔地球内部捕虏体分界面

Using Geographic Information Systems (GIS) for Targeted National Recruitment of Community-Dwelling Caregivers Managing Dementia-Related Behavioral and Psychological Symptoms: A Recruitment Approach for a Randomized Clinical Trial

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Journal of Geographic Information System 2021年第3期13卷 302-317页

作者： Danny l. Scerpella Nicole G. Bouranis Melinda J. Webster Maria Dellapina Sokha Koeuth lauren J. parker Helen C. Kales laura N. Gitlin School of Public Health Johns Hopkins University Baltimore USA College of Nursing and Health Professions Drexel University Philadelphia USA Department of Psychiatry University of California-Davis Sacramento USA

Over 16 million caregivers of people living with dementia require support in a range of issues, including self-care, disease education, and guidance for how to manage behavioral and psychological symptoms of dementia (BPSD). Non-pharmacological interventions are needed to address these areas, and online applications have been shown to be safe and effective. To ensure the efficacy of such interventions, racially, ethnically, geographically, and socioeconomically diverse participants must be recruited to increase the generalizability of study outcomes. This protocol paper describes a recruitment plan using Geographic Information Systems (GIS) to reach a representative sample of caregivers across the United States for a national Phase III clinical study. Using publicly available census data from the American Community Survey (ACS), combined with location data for local aging resources such as Area Agencies on Aging (AAA), recruitment will be derived from data analysis conducted in ESRI ArcGIS v10.7.1. Datasets including age, gender, income, and education will be assessed nationally at the county and census tract spatial scale in a nine-step process to develop recruitment priority areas containing high concentrations of eligible participants living in the community. Overall, the current protocol will demonstrate the value of GIS in tailoring targeted outreach strategies to recruit community-dwelling populations through local resource institutions. This novel approach may have far-reaching implications in future recruitment initiatives and help to secure racially/ethnically diverse samples.

关键词： Geographic Information Systems Recruitment Clinical Trial GIS Dementia or Caregiving

TAlEN-Mediated FlAG-Tagging of Endogenous Histone Methyltransferase DOT1l

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Advances in Bioscience and Biotechnology 2017年第9期8卷 311-323页

作者： Cheng An Guangjing Zhu suzanne N. Martos Xue Feng Haimou Zhang Yankai Jia Zhibin Wang Guang’an Men Hospital China Academy of Chinese Medical Sciences Beijing China laboratory of Human Environmental Epigenomes Department of Environmental Health Sciences Bloomberg School of Public Health Johns Hopkins University Baltimore MD USA School of life Sciences Hubei University Wuhan China GENEWIZ Suzhou Suzhou China Fenxian Central Hospital Shanghai China Department of Oncology and Sidney Kimmel Comprehensive Cancer Center School of Medicine Johns Hopkins University Balti-more MD USA

Histone modification including H3 lysine 79 methylation (H3K79me) plays a key role during gene transcription and DNA damage repair. DOT1l, the sole methyltransferase for three states of H3K79me, is implicated in leukemia, colorectal cancer, and dilated cardiomyopathy. However, understanding of DOT1l and H3K79me in these pathways and disease pathogenesis has been limited due to the difficulty of working with DOT1l protein. For instance, locus-specific or genome-wide binding sites of DOT1l revealed by chromatin immunoprecipitation (ChIP)-based methods are necessary for inferring its functions, but high-quality ChIP-grade antibodies are currently not available. Herein we have developed a knock-in approach to tag endogenous DOT1l with 3 × Flag at its C-terminal domain to follow functional analyses. The knock-in was facilitated by using TAlENs to induce a targeted double-strand break at the endogenous DOTIl to stimulate local homologous recombination at that site. The single cell colonies with successful knock-in were isolated and verified by different methods. We also demonstrated that tagged DOT1l maintains its normal function in terms of methylation and that the engineered cells would be very useful for further studies.

关键词： DOT1l Flag TAlEN Knock-In

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大陆莫霍面是地壳-地慢边界吗?

地震地质译丛

地震地质译丛 1988年第1期 14-18页

作者： W. l. Griffin suzanne Y.O'Reilly 张渤涛

虽然陆壳深部的成分对于地壳-地幔演化的模式而言,是一非常重要的参数,但人们却对其缺少深入的了解。几种这样的模型,根据地球化学的论点和出露的变质粗粒岩体的成分,假设下地壳的总体成分确实是过渡型的。这种模式得到地震折射研究的支... 详细信息

虽然陆壳深部的成分对于地壳-地幔演化的模式而言,是一非常重要的参数,但人们却对其缺少深入的了解。几种这样的模型,根据地球化学的论点和出露的变质粗粒岩体的成分,假设下地壳的总体成分确实是过渡型的。这种模式得到地震折射研究的支持,地震折射的研究提供了在一定深度上,地壳和地幔的密度的间接证据。

关键词：下地壳辉岩莫霍面捕虏体榴辉岩相克拉通稳定地块镁铁质岩石尖晶石二辉橄榄岩边界大陆地貌

Fuel-ion diffusion in shock-driven inertial confinement fusion implosions

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Matter and Radiation at Extremes 2019年第5期4卷 50-54页

作者： Hong Sio Chikang li Cody E.parker Brandon lahmann Ari le Stefano Atzeni Richard D.Petrasso Plasma Science and Fusion Center Massachusetts Institute of TechnologyCambridgeMassachusetts 02139USA los Alamos National laboratory Los AlamosNew Mexico 87545USA Dipartimento SBAI Universita degli Studi di Roma“La Sapienza”Via Antonio Scarpa 1400161RomaItaly

The impact of fuel-ion diffusion in inertial confinement fusion implosions is assessed using nuclear reaction yield ratios and reaction *** T3He-gas-filled(with trace D)shock-driven implosions,the observed TT/T3He yield ratio is∼23lower than expected from temperature ***3He-gas-filled(with trace T)shock-driven implosions,the timing of theD3He reaction history is∼50 ps earlier than those of the DT reaction histories,and average-ion hydrodynamic simulations cannot reconcile this timing *** experimental observations are consistent with reduced T ions in the burn region as predicted by multi-ion diffusion theory and particle-in-cell simulations.

关键词： diffusion shock filled

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