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Grade 4 febrile neutropenia and Fournier's Syndrome associated with triple therapy for hepatitis C virus:A case report

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World Journal of Hepatology 2014年第6期6卷 448-452页

作者： Kelly Cristhian Lima Oliveira Emili de Oliveira Bortolon Cardoso suzana carla pereira de souza Flávia souza Machado Carlos Eduardo Alves Zangirolami Alecsro Moreira Giovanni Faria Silva Cássio Vieira de Oliveira Division of Gastroenterology Department of Internal MedicineBotucatu School of Medicine18618-970 Sao PauloBrazil

The use of triple therapy for hepatitis C not only increases the rate of sustained virological responses compared with the use of only interferon and ribavirin(RBV) but also leads to an increased number of side effects. The subject of this study was a 53-year-old male who was cirrhotic with hepatitis C virus genotype 1 A and was a previous null non-responder. We initially attempted retreatment with boceprevir(BOC), Peginterferon and RBV, and a decrease in viral load was observed in the 8th week. In week 12, he presented with disorientation, flapping, fever, tachypnea, arterial hypotension and tachycardia. He also exhibitedleucopenia with neutropenia. Cefepime and filgrastim were initiated, and treatment for hepatitis C was suspended. A myelogram revealed hypoplasia, cytotoxicity and maturational retardation. After 48 h, he developed bilateral inguinal erythema that evolved throughout the perineal area to the root of the thighs, with exulcerations and an outflow of seropurulent secretions. Because we hypothesized that he was suffering from Fournier's Syndrome, treatment was replaced with the antibiotics imipenem, linezolid and clindamycin. After this new treatment paradigm was initiated, his lesions regressed without requiring surgical debridement. Triple therapy requires knowledge regarding the management of adverse effects and drug interactions; it also requires an understanding of the importance of respecting the guidelines for the withdrawal of treatment. In this case report, we observed an adverse event that had not been previously reported in the literature with the use of BOC.

关键词： Hepatitis C Treatment Boceprevir Telaprevir Adverse events

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Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni

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World Journal of Gastroenterology 2008年第38期14卷 5842-5850页

作者： Sheilla Andrade Oliveira Bruno Solano Freitas souza carla Adriana Guimares-Ferreira Elton Sá Barreto Siane Campos souza Luiz Antonio Rodrigues Freitas Ricardo Ribeiro-dos-Santos Milena Botelho pereira Soares Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Centro de Pesquisas Aggeu Magalhes Fundao Oswaldo Cruz Recife Av Moraes Rego s/n Cidade Universitária Recife 50670-420 PE Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas Salvador 40295-001 BA Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Hospital So Rafael Av So Rafael 2152 So Marcos Salvador 41256-900 BA Brazil

AIM： To investigate the potential of bone marrow mononuclear cells （BM-MCs） in the regeneration of hepatic lesions induced by Schistosoma mansoni （***） chronic infection. METHODS： Female mice chronically infected with *** were treated with BM-MCs obtained from male green fluorescent protein （GFP） transgenic mice by intravenous or intralobular injections. Control mice received injections of saline in similar conditions, Enzyme-linked immunosorbent assay （ELISA） assay for transforming growth factor-beta （TGF-β）, polymerase chain reaction （PCR） for GFP DNA, immunofluorescence and morphometric studies were performed. RESULTS： Transplanted GFP^＋ cells migrated to granuloma areas and reduced the percentage of liver fibrosis. The presence of donor-derived cells was confirmed by Fluorescence in situ hybridization （FISH） analysis for detection of cells bearing Y chromosome and by PCR analysis for detection of GFP DNA. The levels of TGF-β, a cytokine associated with fibrosis deposition, in liver fragments of mice submitted to therapy were reduced. The number of oval cells in liver sections of ***-infected mice increased 3-4 fold after transplantation. A partial recovery in albumin expression, which is decreased upon infection with ***, was found in livers of infected mice after cellular therapy. CONCLUSION： In conclusion, transplanted BMCs migrate to and reduce the damage of chronic fibrotic liver lesions caused by ***.

关键词： Schistosoma mansoni Liver injury Fibrosis Bone marrow cells Transplantation Albumin Oval cells

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Effects of Temperature, Light and Seed Moisture Content on Germination of <i>Euterpe precatoria</i>Palm

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American Journal of Plant Sciences 2018年第1期9卷 98-106页

作者： carla Rafaele Xavier Costa Kathia Fernandes Lopes Pivetta Gilberto Rostirolla Batista de souza Renata Bachin Mazzini-Guedes suzana Targanski Sajovic pereira Marina Romano Nogueira Sao Paulo State University—UNESP Jaboticabal Brazil Federal University of Paraná (UFPR) Jandaia do Sul Brazil

The aim of this study was to investigate the effects of different temperatures, light regimes, and seed moisture contents on germination of the palm Euterpe precatoria, in the Arecaceae family. For the study of light and temperature, the experimental design was entirely randomized;treatments were arranged in a 6 × 2 factorial scheme [six temperature conditions (20°C, 25°C, 30°C, 35°C, 20°C - 30°C, and 25°C - 35°C) combined with two light regimes (light and darkness)] with four replications composed of 25 seeds each. For the seed moisture content study, the experimental design was entirely randomized with five seed moisture contents (27%, 20%, 13%, 12%, and 11%), to which they comprised 5 treatments, and four replications with 25 seeds each. Number of germinated seeds was recorded daily until germination was steady. Data were submitted to variance analysis;for the first experiment, means were compared by the Tukey test (p ≤ 0.05) and, for the second, the regression analysis was performed. The temperature at 20°C promoted highest germination percentage and germination rate for E. precatoria seeds, under either light or darkness;seeds were tolerant up to 11% moisture content.

关键词： Arecaceae Luminosity Ornamental Palm Seed desiccation

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Behavioral Risk Factors for Chronic Non-Communicable Diseases in Adults and Elderly People in the City of Ribeirão Preto—SP

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Journal of Biosciences and Medicines 2022年第12期10卷 42-54页

作者： Jennifer Vieira Paschoalin Marques carla Regina de souza Teixeira Maria Lucia Zanetti Vívian Saraiva Veras Paula Parisi Hodniki Luis Fernando Costa pereira Fundamental Nursing department Escola de Enfermagem de Ribeir&atilde o Preto Universidade de S&atilde o Paulo Ribeir&atilde o Preto Brazil

Aims: to analyze the presence and simultaneity of behavioral risk factors for chronic non-communicable disease (NCD) in adults and the elderly. Methods: quantitative, cross-sectional and observational study. Healthy and unhealthy eating are classified according to the frequency of food consumption. Smoking is expressed by the percentage of smokers. Harmful alcohol consumption by the percentage of individuals who consumed alcoholic beverages at least once in the last 30 days. Classification of the level of physical inactivity required to practice at least 150 minutes of physical activity. Results: the sample constituted 719 people, 535 adults and 184 elderly. The behavioral risk factor reported by most adults 499 (93.3%) and elderly 156 (84.8%) was unhealthy eating. Frequency 72% higher of smoking, four times higher of harmful alcohol consumption and 10% higher of unhealthy food among adults when compared to the elderly. The simultaneity between risk factors was 39% greater in adults than in the elderly. Discussion: risk factors evaluated in this study are key aspects of the development of NCDs. Adults have 2.43 times the chance of presenting two risk factors and 7.73 times the chance of presenting three in relation to the elderly. Conclusion: To achieve more effective and differentiated results, knowing and directing measures to control behavioral risk factors, whether isolated or concurrent, requires specific knowledge.

关键词： Risk Factors Chronic Disease Adult Elderly People Health Unic System

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In Silico Pharmacokinetics Studies for Quinazolines Proposed as EGFR Inhibitors

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Open Journal of Medicinal Chemistry 2015年第4期5卷 106-115页

作者： Gabriela souza Fernandes Michelle Bueno de Moura pereira Ana Cláudia Barbosa Marinho Brisa Machado Ana carla Moreira Matheus Puggina de Freitas Karen Luise Lang Joao Eustáquio Antunes department of Medicine Federal University of Juiz de Fora Governador Valadares Brazil department of Pharmacy Federal University of Juiz de Fora Governador Valadares Brazil department of Chemistry Federal University of Lavras Lavras Brazil

In silico pharmacokinetics studies can aid the search for molecules with potential ability to be drug candidates. In this paper, a number of quinazoline candidates for epidermal growth factor receptor inhibitors—EGFR, important targets for the treatment of cancer, are computationally analyzed. The literature described that 69 quinazoline molecules were synthesized and the respective half maximum inhibitory concentrations (IC50) were obtained. A bilinear parabolic model was built to investigate the druglikeness by correlating the corresponding lipophilicities, which can be represented by the ideal Log P , with the optimal biological activity in terms of pIC50 values. Structural characteristics leading to improved pharmacokinetics parameters were then analyzed. Compound 56 exhibited the lowest IC50 and, therefore, it had the highest ability to inhibit the EGFR. In the present work, the most potent inhibitor 56 is not calculated to be the most promising drug candidate, since it’s out of the parabolic model obtained due to a Log P above 5, which is not within the expected optimum range. Finally, this work is an example of computational prediction that an experimentally, highly active EGFR inhibitor can be unsuccessful as drug candidate because of pitfalls in pharmacokinetics parameters.

关键词： Cancer Treatment Quinazoline Inhibitors Rational Drug design Pharmacokinetics

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Crithidia deanei infection in normal and dexamethasone–immunosuppressed Balb/c mice

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Health 2010年第6期2卷 589-594页

作者： Dilvani Oliveira Santos Saulo C. Bourguignon Helena carla Castro Alice Miranda Rodrigo Tonioni Vieira suzana Corte-Real Otílio Machado pereira Bastos 不详

Monoxenous trypanossomatids protozoa are not believed to cause in vivo infection in verte- brate hosts throughout their life cycle. However, there are reports mentioning some cases of HIV- positive patients who have presented opportunistic infections caused by these protozoa. Recently, we have demonstrated the in vitro infection of mouse dermal fibroblasts by these protozoa. The aim of the present work is to investigate the possibility of Crithidia deanei, a endosymbiont-bearing monoxenous trypanossomatid, infect BALB/c mice under or not dexamethasone treatment. To attend it, distinct gro- ups of adult BALB/c mice were immunosuppressed with 50 mg/kg of dexamethasone. This immunosuppressor was administered 24 hours before infection and daily, for 15 days after C. deanei inoculation. Control groups: C. deanei–inoculated animals but non-immuno- suppressed and non-inoculated animals but immunosuppressed were also used. Light Microscopy analysis revealed an infection process characterized by the presence of the trypanossomatid inside dermal cells in the groups studied. The experimental inoculation resulted in a non-lethal infection characterized by the presence of the trypanossomatid inside dermal cells in the normal BALB/c mice, but notably, in the C. deanei–inoculated immunosuppressed group. These preliminary results lead to the following conclusions: 1) C. deanei is able to infect normal BALB/c mice;2) the immunosupressed mice seemed to be more susceptible to the C. deanei infection compared to the control group. Besides C. deanei in dexamethasone-immunosuppressed mice provides a useful model for studies of monoxenous trypanosomatids ‘in vivo’ infection, resembling that one presumably occurring in imunodeficient individuals with AIDS.

关键词： Monoxenous Trypanossomatid 'In Vivo’ Infection Immunosuppression

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Indolbutyric Acid and Time of the Year Influence on Rooting of Chrysanthemum Cuttings

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American Journal of Plant Sciences 2018年第3期9卷 507-516页

作者： Marina Romano Nogueira Marcos Vieira Ferraz Amanda Kelly Dias Bezerra Renata Bachin Mazzini-Guedes carla Rafaele Xavier Costa Lívia Caroline Praseres de Almeida suzana Targanski Sajovic pereira Kathia Fernandes Lopes Pivetta department of Plant Production Sao Paulo State University (Unesp) Jaboticabal Brazil Federal University of Paraná (UFPR) Jandaia do Sul Brazil

The objective of this study was to analyze the effect of the seasons and indol butyric acid (IBA), as well as the preparation of IBA solutions on rooting growth of “Fine Time” chrysanthemum cuttings. The first experiment was arranged in a 4 × 2 factorial scheme: IBA concentrations (0, 1000, 2000 and 3000 mg·L-1) during summer and winter with 4 replicates of two pots and six cuttings per pot. In the second experiment, the storage of IBA solution (1000 mg·L-1) at 15°C was studied with four replicates of 10 cuttings per plot in a period of four weeks. In the third experiment, dilution and supply of IBA (1000 mg·L-1 or 1000 mg·kg-1) were studied;there were eight treatments: (1) IBA mixed with industrial talc;(2) IBA diluted in 50% alcohol and oven dried at 30°C;(3) IBA diluted in 50% alcohol and oven dried at 70°C;(4) IBA diluted in NaOH and supplied via aqueous solution;(5) IBA diluted in acetone and supplied via aqueous solution;(6) IBA diluted in alcohol 92.8% and supplied via alcoholic solution;(7) IBA diluted in alcohol 50% and supplied via alcoholic solution;(8) IBA mixed and supplied via lanolin;with four replicates of 10 cuttings per plot. Twenty-one days after cutting implementation, the following variables were evaluated: rooting percentage, number, average length and root dry mass. Statistical analysis and time-of-year averages were compared using the Tukey test at 5%;polynomial regression for IBA concentration and preparation data. The means of dilution and delivery were compared by the Scott-Knott test. Treatment with IBA at a concentration of 3000 mg·kg-1 resulted in 90% rooting in the winter and 100% in the summer for all treatments. Results suggest the concentration of 2000 mg·L-1 in the summer and 2000 and 3000 mg·L-1 during the winter provided better quality for the root system. Cuttings treated with IBA diluted in 50% alcohol and oven dried at 30°C showed higher number, length and vigor of the roots.

关键词： dendranthema grandiflora Cutting Asexual Propagation

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The Total Amount of Energy delivered by A Brazilian Hospital Catering does not Meet Patient Requirements as Measured by Indirect Calorimetry

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Food and Nutrition Sciences 2011年第2期2卷 60-65页

作者： Carolina Ferreira Nicoletti Tatiana pereira Lima Roberta deh souza Santos Nancy Yukie Yamamoto Tanaka Vivian Marques Suen Julio Sergio Marchini carla Barbosa Nonino departamento de Clinica Medica Faculdade de Medicina de Ribeirao Preto/USP

The literature is controversial regarding the dietary recommendations for hospitalized patients in Brazil. Objective: We aimed to determine whether the energy content of the diet for hospitalized patients met their energy requirements measured by indirect calorimetry. Methods: Cross-sectional study conducted on adult hospitalized patients of both sexes. The prescription food was checked by reading the charts. Nutritional status was evaluated on the basis of weight, abdominal circumference (AbC), arm circumference (AC), body mass index and waist/hip ratio (WHR). The resting energy requirements of the patients were determined by indirect calorimetry. The diets offered were collected on 3 random non-consecutive days. Total nitrogen, lipid, water, ash and carbohydrate content of the menus were determined. Patients receiving a general or low-sodium diet by the oral route participated in the study. The Student T test was used to analyze the differences in energy expenditure between individuals. The association between gender and percent adequacy of the diet was calculated by the Fisher exact test. The level of significance was set at p ders (29 women and 17 men) aged 45 ± 16 years. Anthropometric evaluation re-vealed a mean weight of 72 ± 26 kg, BMI of 28 ± 10 kg/m2. 78% of the patients received energy above their require-ments, with this value increasing to 82% when obese patients were excluded. Conclusion: Since the hospital diet is an important and often vital resource for the recovery of patients and in view of the high prevalence of undernutrition or obesity, it is fundamental to adjust it to the intra-hospital reality.

关键词： Hospital Diet Nutritional Needs Indirect Calorimetry Hospitalized Patients

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Fungal diversity notes 1277-1386:taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2020年第5期104卷 1-266页

作者： Hai-Sheng Yuan Xu Lu Yu-Cheng Dai Kevin D.Hyde Yu-He Kan Ivana Kušan Shuang-Hui He Ning-Guo Liu V.Venkateswara Sarma Chang-Lin Zhao Bao-Kai Cui Nousheen Yousaf Guangyu Sun Shu-Yan Liu Fang Wu Chuan-Gen Lin Monika C.Dayarathne Tatiana Baptista Gibertoni Lucas B.Conceição Roberto Garibay-Orijel Margarita Villegas-Ríos Rodolfo Salas-Lizana Tie-Zheng Wei Jun-Zhi Qiu Ze-Fen Yu Rungtiwa Phookamsak Ming Zeng Soumitra Paloi Dan-Feng Bao Pranami DAbeywickrama de-Ping Wei Jing Yang Ishara S.Manawasinghe Dulanjalee Harishchandra Rashika S.Brahmanage Nimali Ide Silva Danushka S.Tennakoon Anuruddha Karunarathna Yusufjon Gafforov Dhandevi Pem Sheng-Nan Zhang AndréL.C.Mde Azevedo Santiago Jadson Diogo pereira Bezerra Bálint Dima Krishnendu Acharya Julieta Alvarez-Manjarrez Ali H.Bahkali Vinod K.Bhatt Tor Erik Brandrud Timur S.Bulgakov E.Camporesi Ting Cao Yu-Xi Chen Yuan-Yuan Chen Bandarupalli devadatha Abdallah M.Elgorban Long-Fei Fan Xing Du Liu Gao Camila Melo Gonçalves Luis F.P.Gusmão Naruemon Huanraluek Margita Jadan Ruvishika S.Jayawardena Abdul Nasir Khalid Ewald Langer Diogo X.Lima Nelson Correia de Lima-Júnior carla Rejane Sousa de Lira Jian-Kui(Jack)Liu Shun Liu Saisamorn Lumyong Zong-Long Luo Neven Matočec M.Niranjan JoséRibamar Costa Oliveira-Filho Viktor Papp Eduardo Pérez-Pazos Alan J.L.Phillips Peng-Lei Qiu Yihua Ren Rafael F.Castañeda Ruiz Kamal C.Semwal Karl Soop Carlos A.Fde souza Cristina Maria souza-Motta Li-Hua Sun Meng-Le Xie Yi-Jian Yao Qi Zhao Li-Wei Zhou CAS Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110164People’s Republic of China University of the Chinese Academy of Sciences Beijing 100049People’s Republic of China School of Ecology and Nature Conservation Beijing Advanced Innovation Center for Tree Breeding By Molecular DesignBeijing Forestry UniversityBeijing 100083People’s Republic of China CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Centre of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Mushroom Research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Mai 50150Thailand East and Central Asia Regional Office World Agroforestry Centre(ICRAF)Kunming 650201YunnanChina Ruđer BoškovićInstitute Bijenička cesta 5410000 ZagrebCroatia Faculty of Agriculture Natural Resources and EnvironmentNaresuan UniversityPhitsanulok 65000Thailand department of Biotechnology School of Life SciencesPondicherry UniversityR.V.NagarKalapetPondicherry 605014India College of Biodiversity Conservation Southwest Forestry UniversityKunming 650224People’s Republic of China department of Botany Govt.College UniversityKatchery RoadLahorePakistan State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection Northwest A&F UniversityYanglingShaanxiPeople’s Republic of China Laboratory of Plant Pathology College of Plant ProtectionJilin Agricultural UniversityChangchun 130118Jilin ProvincePeople’s Republic of China Centro de Biociências Departamento de MicologiaUniversidade Federal de PernambucoCidade UniversitáriaAvenida da Engenharia s/nRecifePernambuco 50740-600Brazil departamento de Ciências Biológicas Universidade Estadual de Feira de SantanaAv.Transnordestina s/nNovo HorizonteFeira de SantanaBA 44036-900Brazil L

This is the twelfth contribution to the Fungal Diversity Notes series on fungal taxonomy,based on materials collected from many countries which were examined and described using the methods of morphology,anatomy,and strain culture,combined with DNA sequence analyses.110 taxa are described and illustrated,including five new genera,92 new species,eight new combinations and other taxonomic contributions(one new sequenced species,one new host and three new records)which are accommodated in 40 families and 1 incertae sedis in *** new genera are Amyloceraceomyces,Catenuliconidia,Hansenopezia,Ionopezia and *** new species are Amyloceraceomyces angustisporus,Amylocorticium ellipsosporum,Arthrinium sorghi,Catenuliconidia uniseptata,Clavulina sphaeropedunculata,Colletotrichum parthenocissicola,Coniothyrium triseptatum,Cortinarius indorusseus,***,***,***,***,Diaporthe pimpinel-lae,Dictyosporella guizhouensis,Diplodia torilicola,Fuscoporia marquesiana,***,Hansenopezia decora,Helicoarcta-tus thailandicus,Hirsutella hongheensis,Humidicutis brunneovinacea,Lentaria gossypina,***,Lycoperdon lahorense,***,Magnopulchromyces scorpiophorus,Moelleriella gracilispora,Neodevriesia manglicola,Neodidymelliopsis salvia,***,Neoroussoella magnoliae,Neottiella gigaspora,Ophiosphaerella chiangraiensis,Phaeotremella yunnanensis,Podosphaera yulii,Rigidoporus juniperinus,Rhodofomitopsis pseudofeei,Russula benghalensis,Scleroramularia vermispora,Scytinopogon minisporus,Sporormurispora paulsenii,Thaxteriellopsis obliqus,Tomentella asiae-orientalis,***,***,***,***,***,***,***,***,***-nocystidiata,***,***,***,***,***,***,***,***,***,***,***,***,***,***,***,

关键词： 110 taxa Agaricomycetes Ascomycota Basidiomycota Mucoromycota New combinations New genera New species Phylogeny Taxonomy

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Crithidia deanei infection in normal and dexamethasone–immunosuppressed Balb/c mice

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Health（健康） 2010年第6期

作者： Dilvani Oliveira Santos Saulo C. Bourguignon Helena carla Castro Alice Miranda Rodrigo Tonioni Vieira suzana Corte-Real Otílio Machado pereira Bastos

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