检索结果-南通市图书馆

Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

在线全文

学校读者我要写书评

暂无评论

Animal Nutrition 2022年第4期11卷 1-14页

作者： Jing Tang Yongbao Wu Bo Zhang Suyun Liang Zhanbao Guo Jian Hu Zhengkui Zhou Ming Xie Shuisheng Hou State Key Laboratory of Animal Nutrition Key Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal SciencesChinese Academy of Agricultural Sciences100193BeijingChina

Pantothenic acid deficiency(PAD)in animals causes growth depression,fasting hypoglycemia and impaired lipid and glucose metabolism.However,a systematic multi-omics analysis of effects of PAD on hepatic function has apparently not been reported.We investigated liver proteome and metabolome changes induced by PAD to explain its effects on growth and liver metabolic disorders.Pekin ducks(1-dold,n=128)were allocated into 2 groups,with 8 replicates and 8 birds per replicate.For 16 d,all ducks had ad libitum access to either a PAD or a pantothenic acid adequate(control,CON)diet,formulated by supplementing a basal diet with 0 or 8 mg pantothenic acid/kg of diet,respectively.Liver enlargement,elevated liver glycogen concentrations and decreased liver concentrations of triglyceride and unsaturated fatty acids were present in the PAD group compared to the CON group.Based on integrated liver proteomics and metabolomics,PAD mainly affected glycogen synthesis and degradation,glycolysis and gluconeogenesis,tricarboxylic acid(TCA)cycle,peroxisome proliferator-activated receptor(PPAR)signaling pathway,fatty acid beta oxidation,and oxidative phosphorylation.Selected proteins were confirmed by Western blotting.Downregulation of proteins and metabolites involved in glycogen synthesis and degradation,glycolysis and gluconeogenesis implied that these processes were impaired in PAD ducks,which could have contributed to fasting hypoglycemia,liver glycogen storage,insufficient ATP production,and growth retardation.In contrast,PAD also upregulated proteins and metabolites involved in fatty acid beta oxidation,the TCA cycle,and oxidative phosphorylation processes in the liver;presumably compensatory responses to produce ATP.We inferred that PAD decreased liver triglyceride and unsaturated fatty acids by activating fatty acid beta oxidation and impairing unsaturated fatty acid synthesis.These findings contributed to our understanding of the mechanisms of PAD-induced changes in hepatic metabolism.

关键词： Pantothenic acid Hepatic metabolism Proteomics Metabolomics Hypoglycemia

Efficient protocols for heavy hitter identification with local differential privacy

在线全文

学校读者我要写书评

暂无评论

Frontiers of Computer Science 2022年第5期16卷 193-203页

作者： Dan ZHAO suyun ZHAO Hong CHEN Ruixuan LIU Cuiping LI Wenjuan liang Key Laboratory of Data Engineering and Knowledge Engineering of Ministry of Education Renmin University of ChinaBeijing 100872China School of Information Renmin University of ChinaBeijing 100872China

Local differential privacy(LDP),which is a technique that employs unbiased statistical estimations instead of real data,is usually adopted in data collection,as it can protect every user’s privacy and prevent the leakage of sensitive information.The segment pairs method(SPM),multiple-channel method(MCM)and prefix extending method(PEM)are three known LDP protocols for heavy hitter identification as well as the frequency oracle(FO)problem with large domains.However,the low scalability of these three LDP algorithms often limits their application.Specifically,communication and computation strongly affect their efficiency.Moreover,excessive grouping or sharing of privacy budgets makes the results inaccurate.To address the abovementioned problems,this study proposes independent channel(IC)and mixed independent channel(MIC),which are efficient LDP protocols for FO with a large domains.We design a flexible method for splitting a large domain to reduce the number of sub-domains.Further,we employ the false positive rate with interaction to obtain an accurate estimation.Numerical experiments demonstrate that IC outperforms all the existing solutions under the same privacy guarantee while MIC performs well under a small privacy budget with the lowest communication cost.

关键词： local differential privacy frequency oracle heavy hitter

Effect of riboflavin deficiency on intestinal morphology,jejunum mucosa proteomics,and cecal microbiota of Pekin ducks

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2023年第1期12卷 215-226页

作者： Yaxi Xu Bo Zhang Rui Zhao Kexin Gao Suyun Liang Yongbao Wu Yongsheng Hao Dapeng Liu Zhanbao Guo Jian Hu Zhengkui Zhou Ming Xie Jing Tang State Key Laboratory of Animal Nutrition Key Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China

This study was to determine the effects of riboflavin deficiency(RD)on intestinal development,jejunum mucosa proteome,cecal short-chain fatty acids(SCFA)profiling,and cecal microbial diversity and community of starter Pekin ducks.Male white Pekin ducks(1 d old,n=240)were allocated into 2 groups,with 12 replicates and 10 birds per replicate in each group.For 21 d,all ducks had ad libitum access to either an RD or a riboflavin adequate(control,CON)diet,formulated by supplementing a basal diet with 0 or 10 mg riboflavin per kg of diet,respectively.Compared to the CON group,growth retardation,high mortality,and poor riboflavin status were observed in the RD group.Furthermore,RD reduced the villus height and the ratio of villus height to crypt depth of jejunum and ileum(P<0.05),indicating morphological alterations of the small intestine.In addition,dietary RD enhanced relative cecum weight and decreased cecal SCFA concentrations(P<0.05),including propionate,isobutyrate,butyrate,and isovalerate.The jejunum mucosa proteomics showed that 208 proteins were upregulated and 229 proteins were downregulated in the RD group compared to those in the CON group.Among these,RD mainly suppressed intestinal absorption and energy generation processes such as glycolysis and gluconeogenesis,fatty acid beta oxidation,tricarboxylic acid cycle,and oxidative phosphorylation,leading to impaired ATP generation.In addition,RD decreased the community richness and diversity of the bacterial community in the cecum of ducks.Specifically,RD reduced the abundance of butyrate-producing bacteria in the cecum(P<0.05),such as Eubacterium coprostanoligenes,Prevotella and Faecalibacterium.Dietary RD resulted in growth depression and intestinal hypofunction of Pekin ducks,which could be associated with impaired intestinal absorption and energy generation processes in intestinal mucosa,as well as gut microbiota dysbiosis.These findings contribute to our understanding of the mechanisms of intestinal hypofunction due to RD.

关键词： Riboflavin Deficiency Intestinal morphology Proteomics Cecal microbiota Short-chain fatty acids

Heterogeneous geological conditions and differential enrichment of medium and high maturity continental shale oil in China

在线全文

学校读者我要写书评

暂无评论

Petroleum Exploration and Development 2022年第2期49卷 257-271页

作者： HU suyun BAI Bin TAO Shizhen BIAN Congsheng ZHANG Tianshu CHEN Yanyan liang Xiaowei WANG Lan ZHU Rukai JIA Jinhua PAN Zhejun LI Siyang LIU Yuxi Research Institute of Petroleum Exploration&Development PetroChinaBeijing 100083China Longdong Shale Oil Development Department of PetroChina Changqing Oilfield Qingyang 745100China Key Laboratory of Continental Shale Hydrocarbon Accumulation and Efficient Development Northeast Petroleum UniversityDaqing 163318China

Based on the comparison of basic geological conditions and enrichment characteristics of shale oil plays, the heterogeneity of source and reservoir conditions and differential enrichment of medium-high maturity continental shale oil plays in China have been confirmed.(1) Compared with the homogeneous geological settings and wide distribution of marine shale oil strata in North America, the continental medium and high maturity shale oil plays in China are significantly different in geological conditions generally;continental multi-cyclic tectonic evolution forms multiple types of lake basins in multi-stages, providing sites for large-scale development of continental shale oil, and giving rise to large scale high-quality source rocks, multiple types of reservoirs, and diverse source-reservoir combinations with significant heterogeneity.(2) The differences in sedimentary water environments lead to the heterogeneity in lithology, lithofacies, and organic material types of source rocks;the differences in material source supply and sedimentary facies belt result in reservoirs of different lithologies, including argillaceous and transition rocks, and tight siltstone, and complex source-reservoir combination types.(3) The heterogeneity of the source rock controls the differentiation of hydrocarbon generation and expulsion, the diverse reservoir types make reservoir performance different and the source-reservoir configurations complex, and these two factors ultimately make the shale oil enrichment patterns different. Among them, the hydrocarbon generation and expulsion capacity of high-quality source rocks affect the degree of shale oil enrichment. Freshwater hydrocarbon source rocks with TOC larger than 2.5% and saline hydrocarbon source rocks with TOC of 2% to 10% have a high content of retained hydrocarbons and are favorable.(4) High-abundance organic shale is the basis for the enrichment of shale oil inside the source. In addition to being retained in shale, liquid hydrocarbons migrate along laminae, diagenetic fractu

关键词： continental shale oil medium to high maturity geological characteristics heterogeneity enrichment pattern Junggar Basin Songliao Basin Ordos Basin Santanghu Basin

在线全文

学校读者我要写书评

暂无评论

酸性环境中甲醇电氧化催化剂的研究进展

化工进展 2021年第9期40卷 4962-4974页

作者：余素云梁乐程崔志明华南理工大学化学与化工学院广东广州510641

甲醇的电催化氧化是直接甲醇燃料电池的核心反应,高效、长寿命的阳极催化剂的开发是直接甲醇燃料电池研究的一个重要方向。本文总结了近年来酸性环境中直接甲醇燃料电池阳极催化剂的研究进展,包括甲醇电催化反应机理、催化剂的设计合成... 详细信息

甲醇的电催化氧化是直接甲醇燃料电池的核心反应,高效、长寿命的阳极催化剂的开发是直接甲醇燃料电池研究的一个重要方向。本文总结了近年来酸性环境中直接甲醇燃料电池阳极催化剂的研究进展,包括甲醇电催化反应机理、催化剂的设计合成及其应用。重点介绍了铂基催化剂纳米材料活性和稳定性的增强策略,如组分调控、形貌调控、非金属掺杂以及氧化物的协同催化、载体材料的选用等。最后,对阳极催化剂目前仍存在的制备成本高、催化剂耐久性不足、表征技术有限等问题进行了分析讨论,并对阳极催化剂未来的发展方向进行了展望。

关键词：燃料电池甲醇电催化反应催化剂酸性环境纳米材料

Combining quantitative and qualitative magnetic resonance imaging features to differentiate anorectal malignant melanoma from low rectal cancer

在线全文

学校读者我要写书评

暂无评论

Precision Clinical Medicine 2021年第2期4卷 119-128页

作者： Zeyan Xu Ke Zhao Lujun Han Pinxiong Li Zhenwei Shi Xiaomei Huang Chu Han Huihui Wang Minglei Chen Chen Liu Yanting liang suyun Li Yanqi Huang Xin Chen Changhong liang Wuteng Cao Zaiyi Liu School of Medicine South China University of TechnologyGuangzhou 510006China Department of Radiology Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer MedicineGuangzhou 510060China Department of Radiology Sun Yat-Sen University Cancer CenterGuangzhou 510060China Department of Radiology the Affiliated Hospital of Southwest Medical UniversityLuzhou 646000China The Second School of Clinical Medicine Southern Medical UniversityGuangzhou 510080China Shantou University Medical College Shantou 515041China Guangdong Cardiovascular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Department of Radiology Guangzhou First People’s Hospitalthe Second Affiliated Hospital of South China University of TechnologyGuangzhou 510180China Department of Radiology the Sixth Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510655China

Background:Distinguishing anorectal malignant melanoma from low rectal cancer remains challenging because of the overlap of clinical symptoms and imaging findings.We aim to investigate whether combining quantitative and qualitative magnetic resonance imaging(MRI)features could differentiate anorectal malignant melanoma from low rectal cancer.Methods:Thirty-seven anorectal malignant melanoma and 98 low rectal cancer patients who underwent preoperative rectal MRI from three hospitals were retrospectively enrolled.All patients were divided into the primary cohort(N=84)and validation cohort(N=51).Quantitative image analysiswas performed on T1-weighted(T1WI),T2-weighted(T2WI),and contrast-enhanced T1-weighted imaging(CE-T1WI).The subjective qualitative MRI findings were evaluated by two radiologists in consensus.Multivariable analysis was performed using stepwise logistic regression.The discrimination performance was assessed by the area under the receiver operating characteristic curve(AUC)with a 95%confidence interval(CI).Results:The skewness derived from T2WI(T2WI-skewness)showed the best discrimination performance among the entire quantitative image features for differentiating anorectal malignant melanoma from low rectal cancer(primary cohort:AUC=0.852,95%CI 0.788–0.916;validation cohort:0.730,0.645–0.815).Multivariable analysis indicated that T2WI-skewness and the signal intensity of T1WI were independent factors,and incorporating both factors achieved good discrimination performance in two cohorts(primary cohort:AUC=0.913,95%CI 0.868–0.958;validation cohort:0.902,0.844–0.960).Conclusions:Incorporating T2WI-skewness and the signal intensity of T1WI achieved good performance for differentiating anorectal malignant melanoma from low rectal cancer.The quantitative image analysis helps improve diagnostic accuracy.

关键词： anorectal malignant melanoma low rectal cancer magnetic resonance imaging quantitative image analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

外泌体在多发性骨髓瘤中的研究进展

国际输血及血液学杂志 2022年第4期45卷 353-360页

作者：梁嘉怡王素云广东医科大学研究生院湛江524000 深圳市龙华区中心医院血液内科深圳518000

多发性骨髓瘤(MM)是浆细胞恶性增殖性疾病, 该病的治疗主要以化疗和自体造血干细胞移植(auto-HSCT)为主, 但是仍存在易复发及耐药的问题, 导致患者疗效不佳, 因此现有的治疗手段仍有待进一步改善。外泌体是细胞分泌的微小囊泡。近年越... 详细信息

多发性骨髓瘤(MM)是浆细胞恶性增殖性疾病, 该病的治疗主要以化疗和自体造血干细胞移植(auto-HSCT)为主, 但是仍存在易复发及耐药的问题, 导致患者疗效不佳, 因此现有的治疗手段仍有待进一步改善。外泌体是细胞分泌的微小囊泡。近年越来越多的研究发现, 外泌体可影响MM的发生、发展。因此, 外泌体应用于MM诊疗的研究亦成为该领域的研究热点, 并取得一定进展。为了进一步认识MM中外泌体的作用, 笔者拟就外泌体的分离与鉴定、在MM中的作用机制, 对MM诊断、治疗、耐药的最新研究进展进行综述。

关键词：外泌体多发性骨髓瘤细胞间通讯分子靶向治疗抗药性,肿瘤

在线全文

学校读者我要写书评

暂无评论

基于测序技术的畜禽基因组学研究进展

遗传 2017年第4期39卷 276-292页

作者：梁素芸周正奎侯水生中国农业科学院北京畜牧兽医研究所农业部动物遗传育种与繁殖(家禽)重点实验室北京100193

人类通过数千年的驯化和近代以来有计划性的育种,形成了当今多样化的畜禽品种,从而提供丰富的动物源性蛋白满足人类需求。在过去的100年里,数量遗传学应用于动物育种领域引发了畜禽育种技术的革命,但畜禽机体遗传发育体系相当复杂,一些... 详细信息

人类通过数千年的驯化和近代以来有计划性的育种,形成了当今多样化的畜禽品种,从而提供丰富的动物源性蛋白满足人类需求。在过去的100年里,数量遗传学应用于动物育种领域引发了畜禽育种技术的革命,但畜禽机体遗传发育体系相当复杂,一些性状仍然难以通过基于系谱的育种值进行高效选育,遗传潜能尚未充分发掘。人类基因组计划带来的理念和技术极大促进了畜禽基因组学的发展,使得人们可以从全基因组水平精准定位功能变异,挖掘功能元件的生物学意义,为畜禽分子设计育种提供重要的理论基础。本文对近10年来猪(Sus scrofa)、牛(Bos taurus)、牦牛(Bos grunniens)、山羊(Capra hircus)、绵羊(Ovis aries)、鸡(Gallus gallus)、鸭(Anas platyrhynchos)和鹅(Anser cygnoides)等主要畜禽的基因组学研究进展进行综述,分别从参考基因组构建和群体基因组学分析两个方面进行论述,并对畜禽基因组未来的研究工作进行了展望。

关键词：畜禽参考基因组重测序

RhoA/ROCK-NFκB信号传导通路在内脂素引起心肌细胞肥大中的作用

在线全文

学校读者我要写书评

暂无评论

河北医药 2022年第12期44卷 1799-1803页

作者：常亮杨蓉刘素云李拥军河北省医科大学第二医院心内科石家庄市050000

目的探讨RhoA/ROCK2和NFκb信号传导通路在内脂素引起的心肌细胞肥大中的作用。方法购买出生2~3 d的SD乳鼠,利用差速贴壁法提取原代心肌细胞并进行培养。培养48 h后换为无血清的培养基继续培养24 h,之后48 h改为无血清的培养基继续培养2... 详细信息

目的探讨RhoA/ROCK2和NFκb信号传导通路在内脂素引起的心肌细胞肥大中的作用。方法购买出生2~3 d的SD乳鼠,利用差速贴壁法提取原代心肌细胞并进行培养。培养48 h后换为无血清的培养基继续培养24 h,之后48 h改为无血清的培养基继续培养24 h后,根据实验设计分为不同的干预组。应用Real time-PCR、Western-Blot技术检测不同分组心肌细胞中BNPmRNA及蛋白表达情况,免疫细胞化学法观察NFκB核转位情况。ipp软件计算心肌细胞表面积,Western-Blot技术检测RhoA、ROCK2和IκB-α蛋白表达情况。结果与正常对照组比较,10 ng/ml,50 ng/ml和100 ng/ml内脂素预处理24 h的心肌细胞中,RhoA,ROCK2蛋白表达升高,IκB-α蛋白表达降低(P<0.05,P<0.01)。与正常对照组比较,100 ng/ml内脂素预处理24、48、72 h的心肌细胞中,RhoA ROCK2蛋白表达升高,IκB-α表达降低(P<0.05,P<0.01)。SN50+内脂素、辛伐他汀+内脂素、Y27632+内脂素预处理48 h的心肌细胞表面积,与内脂素干预比较降低(P<0.05,P<0.01),与正常对照组比较升高(P<0.05,P<0.01)。SN50+内脂素、辛伐他汀+内脂素、Y27632+内脂素预处理48h的心肌细胞BNP mRNA和蛋白表达,与内脂素干预比较降低(P<0.05,P<0.01),与正常对照组比较升高(P<0.05,P<0.01)。与正常对照组比较,辛伐他汀、Y27632、SN50予干预组心肌细胞中ROCK2和IκB-α蛋白表达比较差异有统计学意义(P<0.01)。结论内脂素可以通过RhoA/ROCK-NFκB信号传导通路引起心肌细胞肥大。

关键词：内脂素心肌肥大 RhoA/ROCK2和NFκb通路