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Protein & Cell 2015年第7期6卷 529-540页

作者： susu mao State Key Laboratory of Pharmaceutical Biotechnology Nanjing Advanced Institute for Life Sciences (NAILS) Nanjing University School of Life Sciences Nanjing 210093 China Jiangsu Engineering Research Center for microRNA Biology and Biotechnology Nanjing 210093 China Center of Kidney Disease 2nd Affiliated Hospital Nanjing Medical University Nanjing 210003 China

MicroRNAs （miRNAs） are a class of noncoding RNAs that regulates target gene expression at posttranscrip- tional level, leading to further biological functions. We have demonstrated that microvesicles （MVs） can deliver miRNAs into target cells as a novel way of intercellular communication. It is reported that in central nervous system, glial cells release MVs, which modulate neu-ronal function in normal condition. To elucidate the potential role of glial MVs in disease, we evaluated the effects of secreted astrocytic MVs on stress condition. Our results demonstrated that after Lipopolysaccharide （LPS） stimulation, astrocytes released shedding vesi- cles （SVs） that enhanced vulnerability of dopaminergic neurons to neurotoxin. Further investigation showed that increased astrocytic miR-34a in SVs was involved in this progress via targeting anti-apoptotic protein Bcl-2 in dopaminergic neurons. We also found that inhibition of astrocytic miR-34a after LPS stimulation can postpone dopaminergic neuron loss under neurotoxin stress. These data revealed a novel mechanism underlying astrocyte-neuron interaction in disease.

关键词： astrocyte shedding vesicles miR-34a,dopaminergic neurons Bcl-2

MicroRNA-29a modulates axon branching by targeting doublecortin in primary neurons

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Protein & Cell 2014年第2期5卷 160-169页

作者： Hanqin Li susu mao Haitao Wang Ke Zen Chenyu Zhang Liang Li Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical Biotechnology Nanjing University School of Life Sciences Nanjing 210093 China Department of Pharmacy 306th Hospital of PLA Beijing 100101 China

MicroRNAs （miRNAs） are endogenously expressed small, non-coding transcripts that regulate protein expression. Substantial evidences suggest that miRNAs are enriched in central nervous system, where they are hypothesized to play pivotal roles during neural devel- opment. In the present study, we analyzed miRNAs expression in mice cerebral cortex and hippocampus at different developmental stages and found miR-29a increased dramatically at postnatal stages. In addition, we provided strong evidences that miR-29a is enriched in mature neurons both in vitro and in v/vo. Further investigation demonstrated that the activation of gluta- mate receptors induced endogenous miR-29a level in primary neurons. Moreover, we showed that miR-29a directly regulated its target protein Doublecortin （DCX） expression, which further modulated axon branching in primary culture. Together, our results suggested that miR-29a play an important role in neuronal development of mice cerebrum.

关键词： MiRNAs are approximately 21 nucleotide non-codingtranscripts that are derived from hairpin precursors which

Fibroblast exosomal TFAP2C induced by chitosan oligosaccharides promotes peripheral axon regeneration via the miR-132-5p/CAMKK1 axis

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Bioactive Materials 2023年第8期26卷 249-263页

作者： Yahong Zhao Jina Liu Sha Liu Panpan Yang Yunyun Liang Jinyu Ma susu mao Cheng Sun Yumin Yang Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education Co-Innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong University19 Qixiu RoadNantongJiangsu226001China School of Medicine Nantong University19 Qixiu RoadNantongJiangsu226001China

Chitosan and its degradation product,oligosaccharides,have been shown to facilitate peripheral nerve ***,the underlying mechanisms are not well *** this study,we analyzed the protein expression profiles in sciatic nerves after injury using proteomics.A group of proteins related to exosome packaging and transport is up-regulated by chitosan oligosaccharides(COS),implying that exosomes are involved in COS-induced peripheral nerve *** fact,exosomes derived from fibroblasts(f-EXOs)treated with COS significantly promoted axon extension and *** protein identification and functional studies,revealed that TFAP2C is a key factor in neurite outgrowth induced by ***,we showed that TFAP2C targets the pri-miRNA-132 gene and represses miR-132-5p expression in dorsal root ganglion ***1 is a downstream substrate of miR-132-5p that positively affects axon *** rats,miR-132-5p antagomir stimulates CAMKK1 expression and improves axon regeneration and functional recovery in sciatic nerves after *** data reveal the mechanism for COS in axon regeneration,that is COS induce fibroblasts to produce TFAP2C-enriched EXOs,which are then transferred into axons to promote axon regeneration via miR-132-5p/***,these results show a new facet of fibroblasts in axon regeneration in peripheral nerves.

关键词： peripheral nerves chitosan oligosaccharides fibroblast exosomes TFAP2C axon regeneration

miR-20a Promotes the Axon Regeneration of DRG Neurons by Targeting Nr4a3

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Neuroscience Bulletin 2021年第4期37卷 569-574页

作者： Lili Zhao Leilei Gong Ping Li Jing Qin Lingchi Xu Qiyao Wei Huimin Xie susu mao Bin Yu Xiaosong Gu Songlin Zhou Model Animal Research Center and Ministry of Education Key Laboratory of Animal Models of Disease Nanjing UniversityNanjing210000China Key Laboratory of Neuroregeneration of Jiangsu and the Ministry of Education Co-innovation Center of NeuroregenerationNantong UniversityNantong226001China Pathological Anatomy Department Medical CollegeNantong UniversityNantong226001China School of Life Sciences East China Normal UniversityShanghai200241China Medical College Nantong UniversityNantong226001China

Dear Editor,The peripheral nervous system(PNS)regenerates more easily after injury than the central nervous system(CNS)[1].Sensory neurons in the L4–L6 dorsal root ganglia(DRGs)extend axons to form the sciatic nerve ... 详细信息

关键词： Regeneration exceptional injury

MicroRNA-23-27 clusters alleviate neuronal apoptosis though targeting Apaf-1 protein

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MicroRNA-23-27 clusters alleviate neuronal apoptosis though ...

环状RNA circ-Crebbp对Schwann细胞增殖和迁移的影响

中国神经科学学会第十届全国学术会议

作者： Qun Chen Jie Xu Hanqin Li Liang Li susu mao Ke Zen Chen-Yu Zhang Qipeng Zhang Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing University

Apoptotic protease activating factor-1（Apaf1） is a key apoptotic protein and plays a critical role in the regulation of neuronal *** of Apaf1 is gradually decreased during the central nervous system development,which responsible to the decreased caspase ***,the mechanism of decreased expression of Apaf1 in mouse cortex during development remains *** present study,we found that four microRNAs encoded by miR-23a-27a cluster and miR-23b-27b cluster participated in the regulation of Apaf1 *** assay and western blotting assay revealed that these four microRNAs suppressed the expression of Apaf1 in ***,the expression miR-23-27 clusters in mouse cortex are gradually increased,showed a reversed-correlated pattern to the Apaf1 expression *** injury,the most commonly cause of neurological damages in fetus during the perinatal period,could cause neuronal *** examined whether miR-23-27 cluster could attenuate the susceptibility of fetal *** primary neurons,miR-23-27 clusters inhibited the apoptosis induced by hypoxia in vitro;in neuronal specific transgenic mice,over-expression miR-23b-27b cluster inhibited the neuronal apoptosis induced by intrauterine hypoxia in *** together,our results indicate that miR-23-27 clusters are the endogenous inhibitory factors of Apaf1 expression;increased expression of miR-23-27 clusters may control the sensitivity of neurons to *** the first time,we discovered that microRNAs play roles in the neuronal apoptosis during *** study implied that microRNAs might be a potential target to treat the neuronal apoptosis diseases.

关键词： Apafl apoptosis neuron microRNA hypoxia

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南通大学学报（医学版） 2022年第4期42卷 315-320页

作者：姜纯一张珊珊王亚先张竣杰于彬毛苏苏南通大学教育部/江苏省神经再生重点实验室/神经再生协同创新中心南通226001 南通大学附属医院病理科

目的:研究环状RNA circ-Crebbp对Schwann细胞增殖和迁移的影响。方法:(1)采用生物信息学技术对circ-Crebbp序列特性进行分析。(2)提取大鼠坐骨神经总RNA,用RNase R消化,进行聚合酶链式反应(polymerase chain reaction,PCR)扩增和琼脂糖... 详细信息

目的:研究环状RNA circ-Crebbp对Schwann细胞增殖和迁移的影响。方法:(1)采用生物信息学技术对circ-Crebbp序列特性进行分析。(2)提取大鼠坐骨神经总RNA,用RNase R消化,进行聚合酶链式反应(polymerase chain reaction,PCR)扩增和琼脂糖凝胶电泳以证实circ-Crebbp为环状RNA。(3)通过核质分离和实时定量PCR(quantitative real time PCR,qRT-PCR)检测circ-Crebbp在原代Schwann细胞核质中的分布。使用qRT-PCR检测损伤后不同时间点(0、1、4、7、14 d)坐骨神经中circ-Crebbp的表达变化。(4)通过5-乙炔基-2′-脱氧尿苷(5-ethynyl-2′-deoxyuridine,EdU)实验和transwell迁移实验观察circ-Crebbp对Schwann细胞增殖、迁移的影响。结果:(1)circ-Crebbp全长710 nt,由Crebbp基因的2号外显子独立环化形成,主要在Schwann细胞的细胞质中表达。(2)在坐骨神经损伤后,circ-Crebbp表达逐渐升高,到第7天达到最高峰,之后逐渐回复到基础水平。(3)干扰circ-Crebbp的表达后促进了Schwann细胞的增殖、迁移。结论:Circ-Crebbp可以调控坐骨神经损伤后Schwann细胞的增殖和迁移。

关键词：环状RNA circ-Crebbp 坐骨神经损伤 Schwann细胞增殖迁移

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改良序贯法测定氢吗啡酮用于潜伏期硬膜外分娩镇痛的半数有效剂量

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临床麻醉学杂志 2020年第6期36卷 552-554页

作者：张素素毛毛李彩娟沈晓凤徐世琴南京医科大学附属妇产医院(南京市妇幼保健院)麻醉科 210004

目的测定氢吗啡酮用于潜伏期硬膜外分娩镇痛的半数有效剂量(ED50)和90%有效剂量(ED90)。方法选择接受硬膜外分娩镇痛的单胎初产妇28例,年龄25~45岁,BMI 19~29 kg/m2,ASAⅠ或Ⅱ级,宫口扩张≤3 cm。所有产妇于L2-L3间隙行硬膜外腔穿刺并置... 详细信息

目的测定氢吗啡酮用于潜伏期硬膜外分娩镇痛的半数有效剂量(ED50)和90%有效剂量(ED90)。方法选择接受硬膜外分娩镇痛的单胎初产妇28例,年龄25~45岁,BMI 19~29 kg/m2,ASAⅠ或Ⅱ级,宫口扩张≤3 cm。所有产妇于L2-L3间隙行硬膜外腔穿刺并置管,推注氢吗啡酮10 ml。每例产妇所接受的氢吗啡酮剂量按序贯法确定,相邻药物剂量比值为1∶1.2。首例产妇硬膜外注射氢吗啡酮0.4 mg,若VAS疼痛评分≤3分,则下一例产妇接受的氢吗啡酮降低一个剂量梯度;若VAS疼痛评分>3分,则下一例产妇上升一个剂量梯度。记录给药前(T0)及给药后10 min(T1)、20 min(T2)、30 min(T3)时的VAS疼痛评分。计算氢吗啡酮用于潜伏期硬膜外分娩镇痛的ED50和ED90及其95%可信区间(CI)。记录循环抑制、呼吸抑制、恶心呕吐、皮肤瘙痒、寒颤等不良反应的发生情况。结果氢吗啡酮用于潜伏期硬膜外分娩镇痛的ED50为0.237 mg(95%CI 0.206~0.271 mg),ED90为0.479 mg(95%CI 0.320~0.717 mg)。1例产妇出现皮肤轻度瘙痒,无其他不良反应发生。结论氢吗啡酮用于潜伏期硬膜外分娩镇痛的ED50为0.237 mg(95%CI 0.206~0.271 mg),ED90为0.479 mg(95%CI 0.320~0.717 mg),不良反应少,起效时间快。

关键词：分娩镇痛氢吗啡酮硬膜外半数有效剂量 90%有效剂量

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集中带量采购政策对药品供应保障的影响

中国药事 2021年第4期35卷 380-385页

作者：刘苏苏茅宁莹中国药科大学国际医药商学院南京211198

目的:分析集中带量采购政策对药品供应保障存在的隐患,为今后的集采提出相关政策的完善措施。方法:依据药品供应链理论,基于采购政策制度设计和实行效果,探究涉及药品短缺风险的环节。结果:中标价大幅下降,原料药稳定供应存在隐患;独家... 详细信息

目的:分析集中带量采购政策对药品供应保障存在的隐患,为今后的集采提出相关政策的完善措施。方法:依据药品供应链理论,基于采购政策制度设计和实行效果,探究涉及药品短缺风险的环节。结果:中标价大幅下降,原料药稳定供应存在隐患;独家配送不能保障基层地区药品可获得性;较大的地区差价易产生供应隐患,行政倾向的用药方式无法满足患者多样化需求。结论:针对以上影响药品供应保障的风险因素,提出完善相关原料药政策,有序保障中选品种的生产与供给;提高配送商遴选标准,合理规划配送方式提高基层地区配送能力;缩小特殊品种地区间差价,实行梯度降价等方式满足临床用药需求的建议。

关键词：集中带量采购药品短缺药品供应链

水性聚氨酯-丙烯酸酯包覆颜料色浆在涂料染色中的应用

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纺织学报 2018年第9期39卷 71-76,83页

作者：刘素素姜蕾隋晓锋毛志平徐红张琳萍钟毅东华大学生态纺织教育部重点实验室上海201620 东华大学化学化工与生物工程学院上海201620

为提高涂料染色色浆分散稳定性,简化染色工艺流程,以颜料-聚丙烯酸酯为核、聚氨酯为壳,采用无皂乳液聚合法制成了水性聚氨酯-丙烯酸酯包覆颜料色浆,用于棉织物涂料轧染。借助纳米粒度仪与透射电子显微镜对有色乳液平均粒径及分布、颗粒... 详细信息

为提高涂料染色色浆分散稳定性,简化染色工艺流程,以颜料-聚丙烯酸酯为核、聚氨酯为壳,采用无皂乳液聚合法制成了水性聚氨酯-丙烯酸酯包覆颜料色浆,用于棉织物涂料轧染。借助纳米粒度仪与透射电子显微镜对有色乳液平均粒径及分布、颗粒形貌进行分析;通过调节浸轧工艺配方中海藻酸钠用量,改变浸轧次数与焙烘温度优化染色工艺条件。结果表明:水性聚氨酯-丙烯酸酯包覆颜料乳胶粒具有明显的核壳结构,平均粒径为126 nm;染色配方中海藻酸钠质量分数为0.2%,二浸二轧,80℃烘干,140℃焙烘,染色织物得色量较高;经柔软整理,织物手感较好,干摩擦牢度达到4~5级,湿摩擦牢度达到4级,皂洗褪色牢度达到4级。

关键词：水性聚氨酯丙烯酸酯包覆颜料色浆棉织物涂料染色

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两平移一转动并联机器人的控制研究

机床与液压 2007年第12期35卷 48-51页

作者：毛军华高国琴蒯苏苏赵德安江苏大学电气信息工程学院江苏镇江212013

结合中医传统推拿手法,设计了一种两平移一转动的三自由度的并联机构中医推拿机器人。该机器人不仅能模拟中医推拿常用的几种手法,而且结构简单、位置分析求解容易、解耦性强,易于实时控制。在硬件控制回路中,采用模块化处理,使得机器... 详细信息

结合中医传统推拿手法,设计了一种两平移一转动的三自由度的并联机构中医推拿机器人。该机器人不仅能模拟中医推拿常用的几种手法,而且结构简单、位置分析求解容易、解耦性强,易于实时控制。在硬件控制回路中,采用模块化处理,使得机器人组装简便,可移植性好,便于错误排查。在软件设计时,运用VC++6.0多线程技术,实现了远程实时监控的功能。其控制界面简洁、友善、操作方便,不易出错,符合医用界面的要求。

关键词：并联机构位置反解运动分析界面多线程