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Novel CMS lines in pigeonpea[Cajanus cajan(L.)Millspaugh]derived from cytoplasmic substitutions,and their effective restoration and deployment in hybrid breeding

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The Crop Journal 2017年第1期5卷 89-94页

作者： Abhishek Bohra Rintu Jha Indra Prakash Singh Gaurav Pandey Shalini Pareek Partha Sarathi Basu sushil kumar chaturvedi Narendra Pratap Singh ICAR Indian Institute of Pulses Research(IIPR)Kanpur 208024India

The availability of stable cytoplasmic male sterile(CMS or A) lines coupled with a robust restoration system(R lines) is an essential prerequisite for efficient hybrid ***-enabled hybrid technology holds immense potential to enhance the long-stagnant productivity of pigeonpea. In the present investigation, cytoplasmic substitutions were made in the nuclear backgrounds of early-maturing pigeonpea varieties or lines. Three new CMS lines(ICPL 88039 A, Pusa 992 A, and DPP 3-2A) resulted from genetic crosses involving cytoplasmic donors from A2(GT 288A) and A4(ICPA 2089) categories. In addition to visual inspection of anthers, pollen-staining techniques and scanning electron microscopy(SEM)analysis were used to confirm pollen sterility. Further, given the relevance of the plant mitochondrial genome to CMS manifestation, 25 mitochondrion-specific DNA markers were assayed on these newly developed A lines and isogenic maintainer(B) lines. DNA polymorphism between Pusa 992 A and Pusa 992 B as revealed by the nad7a_del marker confirmed the successful combination of sterilizing cytoplasm(A4) and nonrestoring nuclear background(Pusa 992). Such cytoplasm-specific DNA markers are required for A2-CMS as well. Further, to assess restoration ability, potential restorers were crossed with these CMS lines, and as a consequence, promising A × R combinations exhibiting 100%pollen fertility could be identified. In parallel, we also analyzed the inheritance patterns underlying fertility restoration using ICPL 88039A-derived F2 and BC1F1populations,and established a monogenic dominant model to explain the phenomenon of A2-CMS restoration. In summary, we report the successful development of new CMS lines and describe their effective deployment in hybrid breeding of pigeonpea.

关键词： Cytoplasm Hybrid Mitochondria Pigeonpea Restorer Sterility

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Performance validation of High Mountain Asia 8-meter Digital Elevation Model using ICESat-2 geolocated photons

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Journal of Mountain Science 2024年第8期21卷 2562-2578页

作者： Giribabu DANDABATHULA Subham ROY Omkar Shashikant GHATAGE Vaibhav Balaso KOLASE Shwetambari SATPUTE Koushik GHOSH Sahibnoor KAUR Satyanarayana PONDARI Apurba kumar BERA sushil kumar SRIVASTAV Regional Remote Sensing Centre-West National Remote Sensing CentreIndian Space Research OrganisationJodhpur 342005India Department of Geography School of Earth SciencesBharathidasan UniversityTiruchirappalli 620024India Centre for Climate Change and Water Research Suresh Gyan Vihar UniversityJaipur 302017India Department of Geography Panjab UniversityChandigarh 160014India National Remote Sensing Centre Indian Space Research OrganisationHyderabad 500037India Regional Centres National Remote Sensing CentreIndian Space Research OrganisationNew Delhi 110049India

High Mountain Asia(HMA),recognized as a third pole,needs regular and intense studies as it is susceptible to climate *** accurate and high-resolution Digital Elevation Model(DEM)for this region enables us to analyze it in a 3D environment and understand its intricate role as the Water Tower of *** science teams of NASA realized an 8-m DEM using satellite stereo imagery for HMA,termed HMA 8-m *** this research,we assessed the vertical accuracy of HMA 8-m DEM using reference elevations from ICESat-2 geolocated photons at three test sites of varied topography and land *** were made from statistical quantifiers and elevation *** the world’s highest mountain,Mount Everest,and its surroundings,Root Mean Squared Error(RMSE)and Mean Absolute Error(MAE)resulted in 1.94 m and 1.66 m,respectively;however,a uniform positive bias observed in the elevation profiles indicates the seasonal snow cover change will dent the accurate estimation of the elevation in this sort of test *** second test site containing gentle slopes with forest patches has exhibited the Digital Surface Model(DSM)features with RMSE and MAE of 0.58 m and 0.52 m,*** third test site,situated in the Zanda County of the Qinghai-Tibet,is a relatively flat terrain bed,mostly bare earth with sudden river cuts,and has minimal errors with RMSE and MAE of 0.32 m and 0.29 m,respectively,and with a negligible ***,in one more test site,the feasibility of detecting the glacial lakes was tested,which resulted in exhibiting a flat surface over the surface of the lakes,indicating the potential of HMA 8-m DEM for deriving the hydrological *** results accrued in this investigation confirm that the HMA 8-m DEM has the best vertical accuracy and should be of high use for analyzing natural hazards and monitoring glacier surfaces.

关键词： High Mountain Asia Digital Elevation Model ICESat-2 geolocated photons Accuracy assessment

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Genetic Diversity in Selected Indian Mungbean [<i>Vigna radiata</i>(L.) Wilczek] Cultivars Using RAPD Markers

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American Journal of Plant Sciences 2012年第8期3卷 1085-1091页

作者： Subhojit Datta Sarika Gangwar Shiv kumar Sanjeev Gupta Rita Rai Mayank Kaashyap Pallavi Singh sushil kumar chaturvedi Brij Bhuvan Singh Nagaswamy Nadarajan Applied Genomics Laboratory International Crops Research Institute for the Semi Arid Tropics Patancheru India Crop Sciences Division Indian Council of Agricultural Research New Delhi India Indian Institute of Pulses Research Kanpur India International Center for Agricultural Research in the Dry Areas Aleppo Syria Project Coordination Unit MuLLARP Crops Indian Institute of Pulses Research Kanpur India

Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patterns. Amplification of genomic DNA of most popular 24 Indian mungbean cultivars with these RAPD primers yielded 249 fragments that could be scored, of which 224 were polymorphic, with an average of 7.0 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPI 9) to 17 (OPD 7). Percentage polymorphism ranged from 33% (OPX 5) to a maximum of 100% (OPX 4, OPX 6, OPX 13, OPX 15, OPX 19, OPD 5, OPD 7, OPD 20, OPI 4, OPI 6, OPI 13, OPI 14, OPI 18 and OPF 1), with an average of 90%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis. And genotypes grouped in two major groups. Sixteen out of 24 released cultivars grouped to cluster I. This indicated the narrow genetic base in the Indian mungbean cultivars used in the study. The details of diversity analysis and possible reasons for narrow genetic base in mungbean cultivars are discussed in the present study.

关键词： Fingerprinting Genetic Diversity Mungbean RAPD

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Tuning of Optical Properties via Annealing of Bismuth Ferrite (BiFeO3) Thin Films

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Open Journal of Composite Materials 2024年第3期14卷 124-131页

作者： Harish kumar Meena Khushbu Meena Reena Verma Karishma Jain sushil kumar Jain Kedar Babu Sharma Balram Tripathi Department of Physics S S Jain Subodh PG College Jaipur India Department of Physics University of Rajasthan Jaipur India Department of Physics School of Physical Sciences Manipal University Jaipur Jaipur India

In this study we are reporting annealing induced optical properties of bismuth ferrite (BiFeO3) thin films deposited on glass substrate via spin coating at 5000 rpm. The structural, optical and surface morphology of BiFeO3 (BFO) thin films have been studied via X-ray diffraction (XRD), Fourier transform infrared (FT-IR), Optical absorption (UV-Vis) and Photoluminescence (PL) spectroscopy. XRD spectra confirm annealing induced phase formation of BiFeO3 possessing a rhombohedral R3c structure. The films are dense and without cracks, although the presence of porosity in BFO/glass was observed. Moreover, optical absorption spectra indicate annealing induced effect on the energy band structure in comparison to pristine BiFeO3. It is observed that annealing effect shows an intense shift in the UV-Vis spectra as diffuse absorption together with the variation in the optical band gap. The evaluated optical band gap values are approximately equal to the bulk band gap value of BiFeO3.

关键词： Optical Properties Annealing Ferroelectrics Photo Luminescence BFO Thin Films

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Ion emission from warm dense matter produced by irradiation with a soft x-ray free-electron laser

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Matter and Radiation at Extremes 2024年第1期9卷 71-84页

作者： Josef Krása Tomás Burian Vera Hájková Jaromír Chalupský Šimon Jelínek Katerina Frantálová Michal Krupka Zuzana Kuglerová sushil kumar Singh Vojtech Vozda Ludek Vysín MichalŠmíd Pablo Perez-Martin Marion Kühlman Juan Pintor Jakub Cikhardt Matthias Dreimann Dennis Eckermann Felix Rosenthal Sam M.Vinko Alessandro Forte Thomas Gawne Thomas Campbell Shenyuan Ren YuanFeng Shi Trevor Hutchinson Oliver Humphries Thomas Preston Mikako Makita Motoaki Nakatsutsumi Xiayun Pan Alexander Köhler Marion Harmand Sven Toleikis Katerina Falk Libor Juha Department of Radiation and Chemical Physics Institute of PhysicsCzech Academy of SciencesNa Slovance 218221 Prague 8Czech Republic Laser Plasma Department Institute of Plasma PhysicsCzech Academy of SciencesZa Slovankou 318200 Prague 8Czech Republic Department of Surface and Plasma Science Faculty of Mathematics and PhysicsCharles University in PragueV Holesovi ckách 218000 Prague 8Czech Republic Department of Physical Electronics Faculty of Nuclear Science and Engineering PhysicsCzech Technical University in PragueV Holesovi ckách 218000 Prague 8Czech Republic Helmholtz-Zentrum Dresden-Rossendorf Bautzner Landstraße 40001328 DresdenGermany DESY Photon Science Notkestraße 85D-22607 HamburgGermany Institut de Minéralogie de Physique des Matériaux et de CosmochimieUMR 7590–UPMC/CNRS/IRD/MNHNSorbonne Université4 place Jussieu75005 ParisFrance Department of Physics Faculty of Electrical EngineeringCzech Technical University in PragueTechnická216627 Prague 6Czech Republic Center for Soft Nanoscience University of MünsterBusso-Peus-Straße 10D-48149 MünsterGermany Department of Physics Clarendon LaboratoryUniversity of OxfordParks RoadOxford OX13PUUnited Kingdom Central Laser Facility STFC Rutherford Appleton LaboratoryDidcot OX110QXUnited Kingdom Lawrence Livermore National Laboratory 7000 East AvenueLivermoreCalifornia 94550USA European XFEL GmbH Holzkoppel 4D-22869 SchenefeldGermany Technische Universität Dresden 01062 DresdenGermany

We report on an experiment performed at the FLASH2 free-electron laser(FEL)aimed at producing warm dense matter via soft x-ray isochoric *** the experiment,we focus on study of the ions emitted during the soft x-ray ablation process using time-of-flight electron multipliers and a shifted Maxwell–Boltzmann velocity distribution *** find that most emitted ions are thermal,but that some impurities chemisorbed on the target surface,such as protons,are accelerated by the electrostatic field created in the plasma by escaped *** morphology of the complex crater structure indicates the presence of several ion groups with varying *** find that the ion sound velocity is controlled by the ion temperature and show how the ion yield depends on the FEL radiation attenuation length in different materials.

关键词： electrostatic emitted chemi

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An efficient algorithm to clip a 2D-polygon against a rectangular clip window

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Applied Mathematics(A Journal of Chinese Universities) 2022年第1期37卷 147-158页

作者： sushil Chandra Dimri Umesh kumar Tiwari Mangey Ram Department of Computer Science and Engineering Graphic Era(Deemed to be University)DehradunUttarakhandIndia

Polygon clipping is of great importance in computer *** of the popular algorithms to clip a polygon is Cohan–Sutherland Hodgeman algorithm which is based on line ***–Sutherland Hodgeman algorithm clips the polygon against the given rectangular clip window with the help of line clipping ***–Sutherland algorithm requires traversing the polygon in anti clockwise direction(positive orientation).In this work we propose an efficient polygon clipping algorithm against a rectangular clip *** algorithm uses parametric representation of polygon *** the concept of point clipping,we can find required intersection points of edges of polygon with clip window *** suited numerical illustrations are used to explain the proposed polygon clipping *** proposed algorithm is computationally less expensive and comprehensive.

关键词： point clipping polygon clipping clip window line segment computational complexity

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Quantum cascade lasers operating from 1.4 to 4 THz

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Chinese Optics Letters 2011年第11期9卷 9-15页

作者： sushil kumar Department of Electrical and Computer Engineering and Center for Optical Technologies Lehigh University

The development of teranertz （THz） quantum cascade lasers （QCLs） has progressed considerably since their advent almost a decade *** QCLs operating in a frequency range from 1.4 to 4 THz with electron-phonon scattering mediated depopulation schemes are *** different types of GaAs/AlGaAs superlattice designs are *** of the best temperature performances are obtained by the so-called resonant-phonon designs that are *** above a temperature of 160 K has been obtained across the spectrum for THz QCLs operating at ν 〉 1.8 *** maximum operating temperature of previously reported THz QCLs has empirically been limited to a value of ～ω/k B.A new design scheme for THz QCLs with scattering-assisted injection is shown to surpass this empirical temperature barrier,and is promising to improve the maximum operating temperatures of THz QCLs even further.

关键词： Quantum cascade lasers operating from 1.4 to 4 THz QCL

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Surveying the genomic landscape of silage-quality traits in maize(Zea mays L.)

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The Crop Journal 2023年第6期11卷 1893-1901页

作者： Jatin Sharma Shubham Sharma Krishna Sai Karnatam Om Prakash Raigar Chayanika Lahkar Dinesh kumar Saini sushil kumar Alla Singh Abhijit kumar Das Priti Sharma Ramesh kumar Department of Plant Breeding and Genetics Punjab Agricultural UniversityLudhianaPunjab 141004India School of Agricultural Biotechnology Punjab Agricultural UniversityLudhianaPunjab 141004India ICAR-Indian Institute of Maize Research LudhianaPunjab 141004India Department of Plant and Soil Science Texas Tech UniversityLubbockTX 79409-2122USA

Despite the longstanding importance of silage as a critical feed source for ruminants,its quality improvement has been largely *** numerous quantitative trait loci(QTL)and genes affecting silage quality in maize have been reported,only a few have been effectively incorporated into breeding *** this gap,the present study undertook a comprehensive meta-QTL(MQTL)analysis involving 523 QTL associated with silage-quality traits collected from 14 published *** the 523 QTL,405 were projected onto a consensus map comprising 62,424 genetic markers,resulting in the identification of 60 MQTL and eight *** average confidence interval(CI)of the MQTL was 3.9-fold smaller than that of the source *** of the 60 identified MQTL were classified as breeder’s MQTL owing to their small CIs,involvement of more QTL,and large contribution to phenotypic ***-third of the MQTL co-localized with DNA marker-trait associations identified in previous genomewide association mapping studies.A set of 78 high-confidence candidate genes influencing silage quality were identified in the MQTL *** genes and associated markers may advance marker-assisted breeding for maize silage quality.

关键词： Meta-QTL Silage quality Maize GWAS Candidate genes

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An emerging threat Fileless malware:a survey and research challenges

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Cybersecurity 2018年第1期1卷 668-679页

作者： Sudhakar sushil kumar School of Computer&Systems Sciences Jawaharlal Nehru University110067New DelhiIndia Indian Computer Emergency Response Team Ministry of Electronics&Information Technology110003New DelhiIndia

With the evolution of cybersecurity countermeasures,the threat landscape has also evolved,especially in malware from traditional file-based malware to sophisticated and multifarious fileless *** malware does not use traditional executables to carry-out its ***,it does not use the file system,thereby evading signature-based detection *** fileless malware attack is catastrophic for any enterprise because of its persistence,and power to evade any anti-virus *** malware leverages the power of operating systems,trusted tools to accomplish its malicious *** analyze such malware,security professionals use forensic tools to trace the attacker,whereas the attacker might use anti-forensics tools to erase their *** survey makes a comprehensive analysis of fileless malware and their detection techniques that are available in the *** present a process model to handle fileless malware attacks in the incident response *** the end,the specific research gaps present in the proposed process model are identified,and associated challenges are highlighted.

关键词： Fileless malware Botnet Incident response Memory forensics Incident investigation Memory resident malware Rootkit

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Integration of morpho-physico-biochemical traits with SSR and SRAP markers for characterization of castor genotypes of Indian origin

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Oil Crop Science 2022年第1期7卷 22-30页

作者： Akhila S.R sushil kumar Amar A.Sakure Dipak A.Patel Mukesh P.Patel Department of Agricultural Biotechnology Anand Agricultural UniversityAnand388110India Agriculture Research Station Anand Agricultural UniversityKhambholaj388330India

Castor(Ricinus communis L.)is an important tropical oilseed crop,whose oil has versatile,practical value,especially in *** present study aimed to estimate the nature and magnitude of variability in the castor germplasm concerning yield and its component traits and physico-biochemical *** yield per plant and oil content ranged from 80.90 g(ICS-165)to 248.30 g(RG-3216),and 34.7%(ICS-172)to 58.7%(JI-277),*** iodine value of oil ranged from 76.36(JI-370)to 89.84(P2-135)with an average value of *** mean saponification value of oil was *** genotypic and phenotypic coefficients of variation were high for acid value,capsules on the main raceme,seed yield per plant,and total length of the main raceme.A positive association of porosity,average unit volume,and total length of the main raceme with seed yield per plant showed that these characters might be directly attributed to seed yield *** Manhattan distances,the 30 genotypes were grouped into 3 *** genetic diversity was elucidated using SSR and SRAP *** marker produced higher mean number of total bands(5.71),polymorphic bands(4.57),percentage polymorphism(83.10%),PIC(1.72),RP(5.90),mean RP(1.02),MR(5.71),EMR(4.57)and MI(1.44)values when compared to SSR(2.89,2.11,79.63%,0.61,1.90,0.72,2.89,2.11 and 0.49,respectively)*** highest genetic distance(0.77)was between 48-1 and JI-370,which indicated that these genotypes could be used in biparental mating schemes,QTL map development,and hybridization programmes to increase oil content and quality for industrial purposes.

关键词： Diversity DNA marker Heritability Ricinus communis L. Oil

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