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Assessment of Genetic Diversity of Drought Tolerant and Susceptible Rice Genotypes Using Microsatellite Markers

Rice science 2019年第4期26卷 239-247,I0005,I0010-I0014页

作者： Ravindra DONDE Jitendra kumar Gayatri GOUDA Manoj kumar GUPTA Mitadru MUKHERJEE Sk Yasin BAKSH Pradosh MAHADANI Khirod kumar SAHOO Lambodar BEHERA sushanta kumar dash Crop Improvement Division Indian Council of Agricultural Research-National Rice Research Institute/Formerly-Central Rice Research Institute Cuttack Pin-753006.OdishaIndia Ravenshaw University Department of Biotechnology Cuttack Pin-753003Odisha India Department of Biotechnology & Bioinformatics Yogi Vemana University KadapaPin-516003 OdishaIndia

The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472, RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi, RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content (PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.

关键词： genetic diversity marker-assisted selection microsatellite marker rice drought stress submergence chromosome segment substitution line

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Revealing Genetic Relationship and Prospecting of Novel Donors Among Upland Rice Genotypes Using qDTY-Linked SSR Markers

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Rice science 2018年第6期25卷 308-319页

作者： Mitadru MUKHERJEE Barada PADHY Bharathkumar SRINIVASAN Pradosh MAHADANI Sk Yasin BAKSH Ravindra DONDE Onkar Nath SINGH Lambodar BEHERA Padmini SWAIN sushanta kumar dash Crop Improvement Division ICAR-National Rice Research Institute Departement of Botany Kandaswamy Kandar’s College Crop Physiology and Biochemistry Division National Rice Research Institute

A total of 17 simple sequence repeat(SSR) markers linked to QTLs(qDTYs) governing grain yield under reproductive stage(RS) drought stress were used to assess the genetic relationship and prospecting new donors for q DTYs among 32 popular upland rice genotypes. These SSR markers generated a total of 36 alleles with an average allele count of 2.1 per locus. Polymorphic information content value of the markers ranged from 0.376 to 0.662 with an average value of 0.484. The expected heterozyogosity ranged from 0.381 to 0.632. STRUCTURE analysis divided the 32 genotypes into three sub-populations. Subsequent phenotyping revealed that all the tolerant genotypes were grouped into one sub-population, whereas the moderately tolerant and susceptible genotypes were grouped into separate sub-populations. Phylogenetic tree constructed by the unweighted neighbour-joining method also divided the genotypes into three clusters. The grouping pattern of genotypes into the clusters was similar to that into the STRUCTURE analysis, on the basis of drought tolerance level. The average value of genetic dissimilarity coefficient among the genotypes was observed to be 0.486. Furthermore, by combining genotyping data with phenotyping data, 16 new donors for 6 qDTYs were identified.

关键词： drought screening genetic relationship population structure qDTY donor upland rice grain yield

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