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Role of climatic variability in shaping intraspecific variation of thermal tolerance in Mediterranean water beetles

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Insect Science 2024年第1期31卷 285-298页

作者： susana Pallarés David Garoffolo Belén rodríguez David Sánchez-Fernández Department of Zoology University of SevilleSevilleSpain Faculty of Biology Department of Ecology and HydrologyUniversity of MurciaCampus EspinardoMurciaSpain

The climatic variability hypothesis(CVH)predicts that organisms in more thermally variable environments have wider thermal breadths and higher thermal plasticity than those from more stable ***,due to evolutionary trade-offs,taxa with greater absolute thermal limits may have little plasticity of such limits(trade-off hypothesis).The CVH assumes that climatic variability is the ultimate driver of thermal tolerance variation across latitudinal and altitudinal gradients,but average temperature also varies along such *** explored intraspecific variation of thermal tolerance in three typical Mediterranean saline water beetles(families Hydrophilidae and Dytiscidae).For each species,we compared two populations where the species coexist,with similar annual mean temperature but contrasting thermal variability(continental *** population).We estimated thermal limits of adults from each population,previously acclimated at 17,20,or 25℃.We found species-specific patterns but overall,our results agree with the CVH regarding thermal ranges,which were wider in the continental(more variable)*** the two hydrophilid species,this came at the cost of losing plasticity of the upper thermal limit in this population,supporting the trade-off hypothesis,but not in the dytiscid *** results support the role of local adaptation to thermal variability and trade-offs between basal tolerance and physiological plasticity in shaping thermal tolerance in aquatic ectotherms,but also suggest that intraspecific variation of thermal tolerance does not fit a general pattern among aquatic *** such intraspecific variation could lead to inaccurate predictions of the vulnerability of aquatic insects to global warming.

关键词： acclimation capacity aquatic insects climate change heat coma temperature supercooling point thermal plasticity

Chitosan Nanoparticles as Biostimulant in Lettuce (Lactuca sativa L.) Plants

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Phyton-International Journal of Experimental Botany 2024年第4期93卷 777-787页

作者： Silvia C.Ramírez-rodríguez Pablo Preciado-Rangel Marcelino Cabrera-De La Fuente susana González-Morales Hortensia Ortega-Ortiz Tecnológico Nacional de México Instituto Tecnológico de TorreónEjido AnaTorreónCoahuila27170México Departamento de Horticultura Universidad Autónoma Agraria Antonio NarroSaltillo25315México Departamento de Materiales Avanzados Centro de Investigación en Química AplicadaSaltilloCoahuila25294México

Biodegradable nanoparticles such as chitosan nanoparticles (CSNPs) are used in sustainable agriculture since theyavoid damage to the environment;CSNPs have positive effects such as the accumulation of bioactive compoundsand increased productivity in plants. This study aimed to investigate the impact of applying CSNPs on lettuce,specifically focusing on enzymatic activity, bioactive compounds, and yield. The trial was conducted using a completelyrandomized design, incorporating CSNPs: 0, 0.05, 0.1, 0.2, 0.4, and 0.8 mg mL−1. The doses of 0.4 mg mL−1improve yields up to 24.6% increases and 0.1 mg mL−1 of CSNPs increases total phenols by 31.2% and antioxidantcapacity by 34.6%. In addition, when low concentrations of CSNPs (0.05 and 0.1 mg L−1) were applied, anincrease in catalase was determined. The CSNPs represent a good alternative to be used as a biostimulant in sustainableagriculture because they improve the yield and quality of lettuce by increasing the bioactive compounds.

关键词： Biostimulant bioactive compounds enzymatic activity

Output feedback adaptive super twisting sliding mode control for quadrotor UAVs

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Control Theory and Technology 2024年第1期22卷 92-105页

作者： Oscar Salas-peña Jesus DeLeón-Morales susana V.Gutiérrez-Martínez École Centrale de Nantes-LS2N UMR 6004 CNRS44300NantesFrance Facultad de Ingeniería Mecánica y Eléctrica Universidad Autónoma de Nuevo León66451San Nicolás de Los GarzaMexico

In this paper, a sliding mode control with adaptive gain combined with a high-order sliding mode observer to solve the tracking problem for a quadrotor UAV is addressed, in presence of bounded external disturbances and parametric uncertainties. The high order sliding mode observer is designed for estimating the linear and angular speed in order to implement the proposed scheme. Furthermore, a Lyapunov function is introduced to design the controller with the adaptation law, whereas an analysis of finite time convergence towards to zero is provided, where sufficient conditions are obtained. Regarding previous works from literature, one important advantage of proposed strategy is that the gains of control are parameterized in terms of only one adaptive parameter, which reduces the control effort by avoiding gain overestimation. Numerical simulations for tracking control of the quadrotor are given to show the performance of proposed adaptive control–observer scheme.

关键词： Adaptive super twisting controller High-order sliding modes observer UAV

Contribution of glial cells to the neuroprotective effects triggered by repetitive magnetic stimulation:a systematic review

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Neural Regeneration Research 2024年第1期19卷 116-123页

作者： susana A.Ferreira Nuno Pinto Inês Serrenho Maria Vaz Pato Graça Baltazar Centro de Investigação em Ciências da Saúde(CICS-UBI) Universidade da Beira InteriorCovilhãPortugal Faculdade de Ciências da Saúde Universidade da Beira InteriorCovilhãPortugal GRUBI-Systematic Reviews Group University of Beira InteriorCovilhãPortugal

Repetitive transcranial magnetic stimulation has been increasingly studied in different neurological diseases,and although most studies focus on its effects on neuronal cells,the contribution of nonneuronal cells to the improvement trigge red by repetitive transcranial magnetic stimulation in these diseases has been increasingly *** systematically review the effects of repetitive magnetic stimulation on non-neuronal cells two online *** of Science and PubMed were searched fo r the effects of high-frequency-repetitive transcranial magnetic stimulation,low-frequencyrepetitive transcranial magnetic stimulation,intermittent theta-bu rst stimulation,continuous thetaburst stimulation,or repetitive magnetic stimulation on non-neuronal cells in models of disease and in unlesioned animals or cells.A total of 52 studies were *** protocol more frequently used was high-frequency-repetitive magnetic stimulation,and in models of disease,most studies report that high-frequency-repetitive magnetic stimulation led to a decrease in astrocyte and mic roglial reactivity,a decrease in the release of pro-inflammatory cyto kines,and an increase of oligodendrocyte *** trend towards decreased microglial and astrocyte reactivity as well as increased oligodendrocyte proliferation occurred with intermittent theta-burst stimulation and continuous theta-burst *** papers analyzed the low-frequency-repetitive transcranial magnetic stimulation protocol,and the parameters evaluated were restricted to the study of astrocyte reactivity and release of pro-inflammatory cytokines,repo rting the absence of effects on these paramete *** what concerns the use of magnetic stimulation in unlesioned animals or cells,most articles on all four types of stimulation reported a lack of *** is also important to point out that the studies were developed mostly in male rodents,not evaluating possible diffe rential effects of repetitive transcranial magneti

关键词： astrocyte glia high-frequency repetitive magnetic stimulation inflammation low-frequency repetitive magnetic stimulation microglia neurologic disorders oligodendrocyte repetitive magnetic stimulation theta-burst stimulation

Feeding ecology and interactions with mammal hosts in a symbiotic genus of birds(Buphagus spp.)in Namibia

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Avian Research 2024年第3期15卷 336-344页

作者： Michael S.Lukubwe Daniel Velarde-Garcéz Fernando Sequeira susana Lopes Adrian J.F.K.Craig Vanessa A.Mata Department of Zoology and Entomology Rhodes UniversityMakhandaSouth Africa Department of Wildlife Management and Tourism Studies University of NamibiaKatima Mulilo CampusNamibia Departamento de Biologia Faculdade de CiênciasUniversidade do PortoPortoPortugal CIBIO Centro de Investigação em Biodiversidade e Recursos GenéticosInBIO Laboratório AssociadoCampus de VairãoVairãoPortugal BIOPOLIS Program in Genomics Biodiversity and Land PlanningCIBIOCampus de VairãoVairãoPortugal

As the sole obligate symbiotic birds in Africa,oxpeckers offer a unique model for studying symbiotic *** to the multitrophic level they occupy and the context dependent foraging behavior they exhibit,the type of symbiotic relationship can be *** addition to providing a cleaning service to the host by removing ticks,oxpeckers frequently feed on blood,mucus,and saliva,inflicting potential damage on the ***,we used DNA metabarcoding on faecal samples to analyze the taxonomic composition of the trophic interactions of the Yellow-billed Oxpecker(Buphagus africanus)and Red-billed Oxpecker(***)in northeastern *** contrast to conventional methods,DNA metabarcoding allows for a detailed identification of dietary resources encompassing both mammal hosts and consumed arthropods within the same *** this information,we examined differences in the diet composition between oxpecker species and localities,as well as the co-occurrence between host and arthropod *** findings revealed that oxpeckers predominantly source their diet from mammals,ticks,and flies;however,ticks and flies rarely co-occur in the diet of an *** observed variability among individuals in their feeding ecology,which is strongly correlated with locality and,to a lesser extent,with the mammal *** noted a high degree of mobility between hosts within relatively short periods,with 32%of the samples showing traces of at least two mammal *** study illustrates the dynamic foraging behavior of these specialized symbiotic birds,shedding light on their potential role in pest control services and disease transmission.

关键词： Commensalism Diet DNA metabarcoding Oxpeckers Parasitism Trophic ecology

Changes in microhabitat, but not allelopathy, affect plant establishment after Acacia dealbata invasion

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Journal of Plant Ecology 2017年第4期10卷 610-617页

作者： Paula Lorenzo Jonatan rodríguez Luís González susana rodríguez-Echeverría Centre for Functional Ecology(CFE) Department of Life SciencesUniversity of CoimbraCalçada Martim de Freitas3000-456 CoimbraPortugal Facultade de Bioloxía Departamento de Bioloxía Vexetal e Ciencia do SoloUniversidade de VigoAs Lagoas-Marcosende s/n36310PontevedraSpain

Aims The tree legume Acacia dealbata Link is an aggressive Australian invader that severely affects abiotic and biotic compartments of eco-systems *** invasive species outcompetes native plant communities through direct competition,changes in microhabitat and soil properties under the canopy and the release of allelopathic ***,these effects are usually studied separately and under controlled *** objective of this study was to evaluate the combined effect of these modifications exerted by *** on the establishment of native and invasive species in the *** A full factorial experiment was performed in order to test the com-bined effect of microhabitat,soil type and allelopathy on the establish-ment of the invasive *** and three native species in the field(North-West Spain).We sowed seeds of native or invasive species in pots with different soil type(soil collected under *** or Pinus pinaster),allelopathy status(soil treated or untreated with activated car-bon)and microhabitat(under the canopy of *** or ***).The number and total biomass of established plants were evaluated after 8 *** abiotic properties were determined in all *** Findings The establishment of native and invasive species was signifi-cantly affected by the microhabitat,with a higher number of seedlings under the native forest(***).The establishment of *** seedlings was also significantly promoted by its own soil.A negligible effect of allelopathy was detected on the establishment and growth of both invasive and native *** conclude that(i)the main factor affecting seedling establish-ment was microhabitat rather than changes in soil properties or allelopathy,(ii)soil modifications by *** promote the establishment of its own seedlings,contributing to the progress of invasion and(iii)allelochemicals released by *** do not seem to play a key role for the establishment of native

关键词： plant invasion microhabitat soil changes allelopathy plant establishment

Lithium-Metal-Free Sulfur Batteries with Biochar and Steam-Activated Biochar-Based Anodes from Spent Common Ivy

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ENERGY & ENVIRONMENTAL MATERIALS 2024年第6期7卷 118-128页

作者： Salimi, pejman Vercruysse, Willem Chauque, susana Yari, Saeed Venezia, Eleonora Lataf, Amine Ghanemnia, Nahal Zafar, Muhammad Shajih Safari, Mohammadhosein Hardy, An Zaccaria, Remo Proietti Vandamme, Dries Ist Italiano Tecnol Via Morego 30 I-16163 Genoa Italy Univ Genoa Dept Chem & Ind Chem Via Dodecaneso 31 I-16146 Genoa Italy Hasselt Univ Analyt & Circular Chem ACC Martelarenlaan 42 BE-3500 Hasselt Belgium Hasselt Univ Inst Mat Res Imoimomec Martelarenlaan 42 BE-3500 Hasselt Belgium Hasselt Univ Ctr Environm Sci CMK Martelarenlaan 42 BE-3500 Hasselt Belgium Energyville Thor Pk 8310 BE-3600 Genk Belgium IMEC Div IMOMEC Wetenschapspk 1 BE-3590 Diepenbeek Belgium

Lithium-sulfur batteries are emerging as sustainable replacements for current lithium-ion batteries. The commercial viability of this novel type of battery is still under debate due to the extensive use of highly reactive lithium-metal anodes and the complex electrochemistry of the sulfur cathode. In this research, a novel sulfur-based battery has been proposed that eliminates the need for metallic lithium anodes and other critical raw materials like cobalt and graphite, replacing them with biomass-derived materials. This approach presents numerous benefits, encompassing ample availability, cost-effectiveness, safety, and environmental friendliness. In particular, two types of biochar-based anode electrodes (non-activated and activated biochar) derived from spent common ivy have been investigated as alternatives to metallic lithium. We compared their structural and electrochemical properties, both of which exhibited good compatibility with the typical electrolytes used in sulfur batteries. Surprisingly, while steam activation results in an increased specific surface area, the non-activated ivy biochar demonstrates better performance than the activated biochar, achieving a stable capacity of 400 mA h g(-1) at 0.1 A g(-1) and a long lifespan (>400 cycles at 0.5 A g-1). Our results demonstrate that the presence of heteroatoms, such as oxygen and nitrogen positively affects the capacity and cycling performance of the electrodes. This led to increased d-spacing in the graphitic layer, a strong interaction with the solid electrolyte interphase layer, and improved ion transportation. Finally, the non-activated biochar was successfully coupled with a sulfur cathode to fabricate lithium-metal-free sulfur batteries, delivering a specific energy density of similar to 600 Wh kg(-1).

关键词： activated biochar biochar electrochemical performance functional groups lithium-metal-free sulfur batteries ION BATTERIES ELECTROCHEMICAL peRFORMANCE MARINE BIOMASS CARBON ANODES HARD CARBON RICE HUSK ELECTROLYTE PYROLYSIS MICROWAVE LIQUID

An internal control applied to RT-PCR detection of HCV and HIV-1 in human pooled plasma and plasma-derived medicinal products

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BIOCELL 2015年第2期39卷 15-23页

作者： Gonzalo rodrÍguez-LOMBARDI Luciana REYNA María susana VITALI susana GENTI-RAIMONDI Laboratorio de Hemoderivados Universidad Nacional de Córdoba Centro de Investigaciones en Bioquímica Clínica e Inmunología Departamento de Bioquímica ClínicaFacultad de Ciencias QuímicasUniversidad Nacional de Córdoba-Consejo Nacional de Investigaciones Científicas y TécnicasX5000HUA CórdobaArgentina

A competitive internal control(IC)adapted to RT-PCR in-house assay was developed for HCV RNA detection in human pooled ***,it was applied in a multiplex RT-PCR for the HIV-1 and HCV RNA screening in human pooled plasma and plasma-derived products.A 258-bp PCR product from the 5´non-coding region of HCV genome was obtained.A competitive IC template was constructed by inserting a 52-bp double strand sequence into the NheI site of the 258-bp *** sequence was cloned and the obtained plasmid was used to generate a synthetic *** IC/RNA was incorporated in in-house HCV and/or HIV PCR technique to monitor the efficiency of extraction,reverse transcription,and PCR amplification *** was also used to detect all major genotypes of HCV and HIV-1 strains with similar *** detection limit of the assay for HCV and HIV-1 was 52.7 IU/mL and 164.2 IU/mL,*** techniques have been evaluated in international programs of external quality assurance with highly satisfactory *** IC is an essential reagent in PCR techniques to detect and identify HCV and HIV-1 in pooled plasma samples involved in the manufacture of plasma-derived products as well as in the field of clinical microbiology with limited resources.

关键词： HCV HIV internal control RT-PCR

Effectiveness of ivermectin mass drug administration in the control of soil-transmitted helminth infections in endemic populations: a systematic review and meta-analysis

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Infectious Diseases of Poverty 2024年第1期13卷 1-15页

作者： Brandon Le Naomi E.Clarke Nicolas Legrand susana Vaz Nery The Kirby Institute University of New South WalesLevel 6Wallace Wurth BuildingSydneyNSW2052Australia

Background Current soil-transmitted helminth(STH)control guidelines endorse the use of albendazole or meben-dazole for school-based targeted preventive chemotherapy(PC),yet their reduced efficacy against Strongyloides sterc-oralis and Trichuris trichiura presents significant *** evidence indicates that community-wide PC[or mass drug administration(MDA)]using ivermectin,commonly used in other neglected tropical disease(NTD)control programs,may play an important role in controlling these *** conducted a systematic review and meta-analysis to evaluate the effectiveness of ivermectin PC in reducing STH prevalence in endemic *** We searched Pubmed,EMBASE,and Web of Science on February 14,2023,for studies that investigated the effectiveness of ivermectin PC,either alone or in combination with other anthelmintic drugs,on STH infec-tions,and provided a measure of STH prevalence before and after *** calculated pooled prevalence reductions for each STH using random-effects *** protocol is available on PROSpeRO(registration number CRD42023401219).Results A total of 21 were eligible for the systematic review,of which 15 were eligible for *** studies delivered ivermectin through *** pooled prevalence reduction of *** following MDA with ivermec-tin alone was 84.49%(95%CI:54.96-94.66)across five studies and 81.37%(95% CI:61.62-90.96)across seven studies with or without *** prevalence reduction of *** was 49.93%(95%CI:18.23-69.34)across five studies with ivermectin alone,and 89.40%(95%CI:73.66-95.73)across three studies with the addition of *** was high heterogeneity for all syntheses(I^(2)>65%).Conclusions This study underscores the key role of ivermectin-based MDA in addressing limitations in current global STH guidelines in terms of limited efficacy against *** and *** on these findings,revising inter-national STH guidelines to include i

关键词： Soil-transmitted helminths Ivermectin Albendazole Mass drug administration Preventive chemotherapy