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Correlation of serum SARS-CoV-2 IgM and IgG serology and clinical outcomes in COVID-19 patients:Experience from a tertiary care centre

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World Journal of Biological Chemistry 2023年第2期14卷 52-61页

作者： Mohan suresh Pratap Kumar Prasan Kumar Panda Vikram jain Rohit Raina Sarama Saha Subbiah Vivekanandhan Balram Ji Omar Department of Internal Medicine All India Institute of Medical SciencesRishikesh 249203India Department of Biochemistry All India Institute of Medical SciencesRishikesh 249203India Department of Microbiology All India Institute of Medical SciencesRishikesh 249203India

BACKGROUND The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus has become a pandemic for the last 2 *** response to the virus leads to organ dysfunction and *** the severity of inflammatory response helps in managing critical patients using serology tests IgG and *** To investigate the correlation of the serology(IgM and IgG)with reverse transcriptase polymerase chain reaction(RT-PCR)status,disease severity[mild to critical],intensive care unit(ICU)admission,septic shock,acute kidney injury,and in-hospital *** We conducted a longitudinal study to correlate serum SARS-CoV-2 immunoglobulin M(IgM)and immunoglobulin G(IgG)serology with clinical outcomes in coronavirus disease 2019(COVID-19)*** analyzed patient data from March to December 2020 for those who were admitted at All India Institute of Medical Sciences *** and laboratory data of these patients were collected from the e-hospital portal and analyzed.A correlation was seen with clinical outcomes and was assessed using MS Excel 2010 and SPSS *** Out of 494 patients,the mean age of patients was 48.95±16.40 years and there were more male patients in the study(66.0%).The patients were classified as mild-moderate 328(67.1%),severe 131(26.8%),and critical 30(6.1%).The mean duration from symptom onset to serology testing was 19.87±30.53 ***-hospital mortality was observed in 25.1%of *** seropositivity rate(i.e.,either IgG or IgM>10 AU)was 50%.IgM levels(AU/mL)(W=33428.000,P≤0.001)and IgG levels(AU/mL)(W=39256.500,P≤0.001),with the median IgM/IgG levels(AU/mL),were highest in the RT-PCR-Positive group compared to RT-PCR-Negative clinical *** was no significant difference between the two groups in terms of all other clinical outcomes(disease severity,septic shock,ICU admission,mechanical ventilation,and mortality).CONCLUSION The study showed that serology levels are high in RT-PCR positive group compared

关键词： Inflammatory response Reverse transcription polymerase chain reaction SARS-CoV-2 Serology IgM and IgG

Estimation of Area under Receiver Operating Characteristic Curve for Bi-Pareto and Bi-Two Parameter Exponential Models

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Open Journal of Statistics 2014年第1期4卷 1-10页

作者： Bhavna Kaushal Kanchan jain suresh K. Sharma Department of Statistics Panjab University Chandigarh India

In this paper, we find the ROC curves for Bi-Pareto and Bi-two parameter exponential distributions. Theoretical, parametric and non-parametric values of area under receiver operating characteristic (AUROC) curve for different parametric combinations have been calculated using simulations. These values are compared in terms of root mean square and mean absolute errors. The results are demonstrated for two real data sets.

关键词： ROC AUROC Pareto Two Parameter Exponential Root Mean Square Error Mean Absolute Error

Tin Sulfide Nanoparticle Synthesis from Waste Waters

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American Journal of Analytical Chemistry 2014年第2期5卷 50-54页

作者： Jitender Gaur Shilpa jain suresh Chand Narender Kumar Kaushik Department of Chemistry University of Delhi New Delhi India Department of Chemistry University of Delhi New Delhi India J & S Research and Innovations New Delhi India Department of Chemistry University of Delhi New Delhi India J & S Research and Innovations New Delhi India 3Physics of Energy Harvesting CSIR-National Physical Laboratory New Delhi India Physics of Energy Harvesting CSIR-National Physical Laboratory New Delhi India

Tin and its derivatives are extensively used in modern technology tools which lead requirement for development of green methods for its waste management and profitable recycling. Presence of tin in water bodies causes enormous environmental concern due to its acute toxicity to living beings. We demonstrate a simple and zero emission process for extraction of tin ions from aqueous solution using dithiocarbamate-based copolymer and its single step quantitative recovery and transformation into SnS nanoparticles. The polymer shows high metal extraction capacity, 1.06 g SnCl2 per gram of polymer. The polymer metal composite and SnS nanoparticles are analyzed using TEM, FTIR, XRD spectroscopy techniques. The FTIR shows formation of Sn-S bonding in the polymer metal composite. This study has implication for cost effective and green approach for purification of water and waste management.

关键词： Tin Sulfide Nanoparticles Metal Extraction Green Chemistry Hydrothermal Synthesis Water Purification

Mediastinal Neuro-Fibro-Lipoma with Atypia: A Case Report

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Open Journal of Radiology 2014年第1期4卷 97-100页

作者： Kishor Taori suresh Dhakate Jawhar Rathod Amit Disawal Anand Hatgoankar Rohit Khisti Mansi jain Shashikant Mane Rahul jain Department of Radiodiagnosis Government Medical College Nagpur India

Mediastinal lipomatous tumors with additional malignant soft tissue components are exceedingly rare. Patients can have substantially large sized tumors with long duration of symptoms and can be misinterpreted on radiographs. Enhancing soft tissue component within a fat density lesion within the mediastinum is alarming and should raise the suspicion of sarcomatous component. Along with diagnostic imaging, selective CT guided biopsy/FNAC from the enhancing soft tissue component can help in making correct diagnosis.

关键词： Mediastinal Neuro-Fibro-Lipoma CT Scan

Prenatal Diagnosis of Fetus in Fetu with a Well Formed Skull: A Rare Case Report

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Open Journal of Obstetrics and Gynecology 2014年第12期4卷 710-715页

作者： Kishor Taori suresh Dhakate Ramesh Parate Jawhar Rathod Amit Disawal Anand Hatgaonkar Rahul jain Akshat Kasat Dinesh Kumar Government Medical College Nagpur India

Fetus in fetu is an extremely rare condition embryologically arising in monozygotic diamniotic twins with an incidence of 1:500,000 births. To the best of our knowledge, number of reported cases should not be more than 100 with majority of cases presenting in post natal life with only 5 cases detected prenatally. We are presenting a case diagnosed at 24 weeks of gestation on ultrasound with presence of well formed skull, facial skeleton and bony orbit.

关键词： Fetus in Fetu Prenatal Diagnosis

Rauvolfia serpentina-Mediated Green Synthesis of CuO Nanoparticles and Its Multidisciplinary Studies

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Acta Metallurgica Sinica(English Letters) 2015年第9期28卷 1134-1140页

作者： K.Lingaraju H.Raja Naika K.Manjunath G.Nagaraju D.suresh H.Nagabhushana Department of Studies and Research in Environmental Science Tumkur University Centre for Nano and Material Sciences Jain University Department of Chemsitry Siddaganga Institute of Technology Department of Studies and Research in Chemistry Tumkur University Prof. C.N.R. Rao Center for Advanced Materials Tumkur University

Copper oxide nanoparticles （CuO Nps） were successfully synthesized by solution combustion method using aqueous leaf extract of Rauvolfia serpentina as a fuel. The structure and morphology of the CuO nanoparticles （Nps） were characterized by powder X-ray diffraction （PXRD）, UV-visible spectroscopy （UV-visible）, scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, etc. The PXRD patterns reveal the formation of monoclinic phase with crystallite structure. SEM images indicate that the particles have sponge-like structure being highly porous and agglom- erated with large surface area. The average crystallite sizes were found to be in the range of 10-20 nm by Scherrer＇s method. The CuO Nps size was further confirmed by TEM. Further, CuO Nps exhibit good photocatalytic activity for the photodegradation of trypan blue dye, indicating that it acts as a promising semiconducting material. The antibacterial properties of CuO nanoparticles were investigated against pathogenic bacterial strains, namely Gram -ve Escherichia coli （NCIM-5051） and Pseudomonas desmolyticum （NCIM-2028） and Gram ＋ve bacteria Staphylococcus aureus （NCIM- 5022） using the agar well diffusion method.

关键词： CuO Nanoparticles Rauvolfia serpentina Photocatalytic activity Antibacterial activity

Design and development of a plastic scintillator based whole body β/γ contamination monitoring system

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Nuclear Science and Techniques 2021年第5期32卷 33-42页

作者： Vaishali M.Thakur Amit jain P.Ashokkumar Rekha Anilkumar Pravin Sawant Probal Chaudhury L.M.Chaudhari Radiation Safety Systems Division Bhabha Atomic Research CentreMumbai 400085India Health Physics Division Bhabha Atomic Research CentreMumbai 400085India Department of Physics Nowrosjee Wadia CollegePuneMaharashtra 411001India

Plastic scintillation detectors based whole body β/γ contamination monitors are developed for use in radiation *** microcontroller-based multi-detector system uses 13 plastic scintillator detectors,with minimized dead detection zones,monitoring the whole body,and conforming to the contamination limit prescribed by the regulatory *** system has the features for monitoring hands,feet,head,and faceβ/γusing contamination monitors and portal exit *** can detect gamma sources at a dose rate of 10 n Gyh^(-1).The system is calibrated using b sources^(90)Sr/^(90)Y,^(204)Tl,and^(36)Cl,and the efficiency is found to be 29%,22%,and 18%,***β/γcontaminationis0.15 Bqcm^(-2),which is significantly less than the minimum detection objectives on head,face,hands,and feet.

关键词： Plastic scintillation detector Contamination monitor Microcontroller Minimum Detection Objective(MDO) Minimum Detection Concentration(MDC)

Protective effect of diosmin against diabetic neuropathy in experimental rats

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Journal of Integrative Medicine 2014年第1期12卷 35-41页

作者： Dilpesh jain Manish Kumar Bansal Rahul Dalvi Aman Upganlawar Rahul Somani Department of Pharmacology Sinhgad College of Pharmacy Department of Pharmacology SSDJ College of Pharmacy Department of Research Suresh Gyan Vihar University

OBJECTIVE： The present study was undertaken to evaluate the effect of diosmin in diabetic neuropathy in type 2 diabetic rats. METHODS： Type 2 diabetes was induced in male Sprague-Dawley rats by single intraperitoneal injection of streptozotocin （35 mg/kg） and high-fat diet. Four weeks after the confirmation of diabetes, diabetic rats were treated with diosmin （50 and 100 mg/kg, p.o.） for next 4 weeks. Rats were evaluated for biochemical, behavioral and oxidative stress parameters. Eddy＇s hot plate and tail immersion test were performed on 6th, 7th, 8th, 9th and 10th weeks of experiment to assess thermal hyperalgesia and cold allodynia respectively. Further, the walking function test was performed for assessing the motor responses at the end of the treatment schedule. RESULTS： Rats were fed with high-fat diet throughout the experiment schedule and administration of low-dose streptozotocin induced significant elevation in blood glucose level and insulin resistance which was confirmed by oral glucose tolerance test. Treatment with diosmin at doses of 50 and 100 mg/kg significantly restored the reduced body weight, elevated blood sugar and lipid profiles. Further the dose-dependent improvement was observed in thermal hyperalgesia, cold allodynia and walking function in diabetic rats treated with diosmin. Elevated levels of malondialdehyde, and nitric oxide and decreased glutathione levels and superoxide dismutase activity in diabetic rats were restored significantly after the 4 weeks of diosmin treatment. CONCLUSION： Diosmin has shown beneficial effect in preventing the progression of early diabetic neuropathy in rats.

关键词： diosmin plant extracts diabetic neuropathies diabetes mellitus, type 2 rats

Green Approach for <i>In-Situ</i>Growth of CdS Nanorods in Low Band Gap Polymer Network for Hybrid Solar Cell Applications

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Advances in Nanoparticles 2014年第3期3卷 106-113页

作者： Ramil K. Bhardwaj Vishal Bharti Abhishek Sharma Dibyajyoti Mohanty Vikash Agrawal Nakul Vats Gauri D. Sharma Neeraj Chaudhary Shilpa jain Jitender Gaur Kamalika Banerjee suresh Chand J & S Research and Innovations Delhi India Physics of Energy Harvesting CSIR-National Physical Laboratory New Delhi India Physics of Energy Harvesting CSIR-National Physical Laboratory New Delhi India School of Science India Gandhi National Open University New Delhi India School of Science India Gandhi National Open University New Delhi India

In-situ growth of CdS nanorods (NRs) has been demonstrated via solvothermal, in a low band gap polymer, poly [[4,8-bis[(2-ethylhexyl)oxy] benzo [1,2-b:4,5-b’] dithiophene-2,6-diyl] [3-fluoro-2-[(2-ethylhexyl) carbonyl] thieno [3,4-b] thiophenediyl]] (PTB7). It is a high yielding, green approach as it removes use of volatile and hazardous chemicals such as pyridine as ligand which are conventionally used to synthesize precursors of CdS (NRs). Moreover the solvothermal process is a zero emission process being a close vessel synthesis and hence no material leaching into the atmosphere during the synthesis. The PTB7:CdS nanocomposite has been characterized by SEM, XRD, FTIR, UV-visible spectroscopy techniques. The photoluminescence (PL) spectroscopy study of PTB7 with CdS NRs has shown significant PL quenching by the incorporation of CdS NRs in PTB7;this shows that CdS NRs are efficient electron acceptors with the PTB7. The PTB7:CdS is used as active layer in the fabrication of hybrid solar cells (HSC) as donor-acceptor combination in the bulk heterojunction (BHJ) geometry. The HSCs fabricated using this active layer without any additional supporting fullerene based electron acceptor has given power conversion efficiency of above 1%.

关键词： CdS Nanorods PTB7 In-Situ Growth Solvothermal Hybrid Solar Cell