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Genome-Wide Identification and Functional Analysis of Genes Expressed Ubiquitously in Rice

Molecular Plant 2015年第2期8卷 276-289页

作者： Ki-Hong Jung sung-ruyl kim Hoi-Khoanh Giong Minh Xuan Nguyen Hyun-Jung Koh Gynheung An Department of Plant Molecular Systems Biotechnology & Crop Biotech Institute Kyung Hee University Yongin 446-701 Korea Graduate School of Biotechnology Kyung Hee University Yongin 446-701 Korea These authors contributed equally to the article.

Genes that are expressed ubiquitously throughout all developmental stages are thought to be necessary for basic biological or cellular functions. Therefore, determining their biological roles is a great challenge. We identified 4034 of these genes in rice after studying the results of Agilent 44K and Affymetrix meta- anatomical expression profiles. Among 105 genes that were characterized by loss-of-function analysis, 79 were classified as members of gene families, the majority of which were predominantly expressed. Using T-DNA insertional mutants, we examined 43 genes and found that loss of expression of six genes caused developing seedor seedling-defective phenotypes. Of these, three are singletons without similar family members and defective phenotypes are expected from mutations. Phylogenomic analyses integrating genome-wide transcriptome data revealed the functional dominance of three ubiquitously expressed fam- ily genes. Among them, we investigated the function of OsO3g19890, which is involved in ATP generation within the mitochondria during endosperm development. We also created and evaluated functional net- works associated with this gene to understand the molecular mechanism. Our study provides a useful strategy for pheonome analysis of ubiquitously expressed genes in rice.

关键词： functional redundancy phylogenomic analysis rice systematic phenotype screening ubiquitouslyexpressed genes

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Development and application of a multi-step porcine in vitro system to evaluate feedstuffs and feed additives for their efficacy in nutrient digestion, digesta characteristics, and intestinal immune responses

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Animal Nutrition 2024年第2期17卷 265-282页

作者： Hee Yeon kim Jun-Ok Moon sung Woo kim Application Center CJ Blossom Park Department of Animal Science North Carolina State University

In vitro model provides alternatives to the use of live animals in research. In pig nutrition, there has been a tremendous increase in in vivo research over the decades. Proper utilization of in vitro models could provide a screening tool to reduce the needs of in vivo studies, research duration, cost, and the use of animals and feeds. This study aimed to develop a multi-step porcine in vitro system to simulate nutrient digestion and intestinal epithelial immune responses affected by feedstuffs and feed additives. Seven feedstuffs(corn, corn distillers dried grains with solubles [corn DDGS], barley, wheat, soybean meal, soy protein concentrates, and Corynebacterium glutamicum cell mass [CGCM]), feed enzymes(xylanase and phytase), and supplemental amino acids(arginine, methionine, and tryptophan), were used in this in vitro evaluation for their efficacy on digestibility, digesta characteristics, and intestinal health compared with the results from previously published in vivo studies. All in vitro evaluations were triplicated. Data were analyzed using Mixed procedure of SAS9.4. Evaluations included(1) nutrient digestibility of feedstuffs,(2) the effects of feed enzymes, xylanase and phytase, on digestibility of feedstuffs and specific substrates, and(3) the effects of amino acids, arginine, tryptophan, and methionine, on anti-inflammatory, anti-oxidative, and anti-heat stress statuses showing their effects(P < 0.05) on the measured items. Differences in dry matter and crude protein digestibility among the feedstuffs as well as effects of xylanase and phytase were detected(P < 0.05), including xylo-oligosaccharide profiles and phosphorus release from phytate. Supplementation of arginine, tryptophan, and methionine modulated(P < 0.05) cellular inflammatory and oxidative stress responses. The use of this in vitro model allowed the use of 3 experimental replications providing sufficient statistical power at P < 0.05. This indicates in vitro models can have increased prec

关键词： Digestion Feed additive Feedstuff In vitro Intestine Pig

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Soleus arthrogenic muscle inhibition following acute lateral ankle sprain correlates with symptoms and ankle disability but not with postural control

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Journal of Sport and Health Science 2024年第4期13卷 559-568,F0003页

作者： Kyung-Min kim Joo-sung kim Alan R.Needle Department of Sport Science Sungkyunkwan UniversitySuwon-si 16419Republic of Korea Department of Health and Human Performance Texas State UniversitySan MarcosTX 78666USA Department of Public Health&Exercise Science Appalachian State UniversityBooneNC 28608USA Department of Rehabilitation Science Appalachian State UniversityBooneNC 28608USA

Background:Acute lateral ankle sprains(ALAS)are associated with long-term impairments and instability tied to altered neural *** muscle inhibition(AMI)has been observed in this population;however,relationships with injury-related impairments are unclear,potentially due to the resting,prone position in which AMI is typically *** AMI during bipedal stance may provide a better understanding of this ***:AMI was assessed in 38 young adults(19 ALAS within 72 h of injury:10 males,21.4±2.7 years;19 healthy controls:10 males,21.9±2.2 years;mean±SD)using the Hoffmann reflex(H-reflex)during bipedal *** stimulation was administered to identify the _(max)imal H-reflex(H_(max))and _(max)imal motor response(M_(max))from the soleus,fibularis longus,and tibialis anterior *** primary outcome measure was the H_(max)/M_(max) *** outcomes included acute symptoms(pain and swelling),postural control during bipedal stance,and self-reported ***:No significant group-by-limb interactions were observed for any ***,a significant group main effect was observed in the soleus muscle(F(1,35)=6.82,p=0.013),indicating significantly lower H_(max)/M_(max) ratios following ALAS(0.38±0.20)compared to healthy controls(0.53±0.16).Furthermore,lower H_(max)/M_(max) ratios in the soleus significantly correlated with acute symptoms and self-reported function but not with postural ***:This study supports previous evidence of AMI in patients with ALAS,providing insight into neurophysiologic impacts of musculoskeletal *** results suggest that assessing AMI in a standing position following acute injury may provide valuable insight into how AMI develops and guide potential therapeutic options to curb and offset the formation of joint instability.

关键词： Acute injuries Balance Hoffmann reflex Neural excitability Patient-reported outcomes

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Comparison of commercial silicon-based anode materials for the design of a high-energy lithium-ion battery

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Nano Research 2024年第6期17卷 5270-5277页

作者： Minhong Choi Eunhan Lee Jaekyung sung Namhyung kim Minseong Ko Division of Convergence Materials Engineering Pukyong National UniversityBusan 48547Republic of Korea Department of Materials Engineering and Convergence Technology Gyeongsang National University501 Jinju-daeroJinju 52828Republic of Korea Department of Materials System Engineering Pukyong National UniversityBusan 48547Republic of Korea

Silicon(Si)is considered a potential alternative anode for next-generation Li-ion batteries owing to its high theoretical capacity and ***,the commercial use of Si anodes is hindered by their large volume expansion(~300%).Numerous efforts have been made to address this *** these efforts,Si-graphite co-utilization has attracted attention as a reasonable alternative for high-energy anodes.A comparative study of representative commercial Si-based materials,such as Si nanoparticles,Si suboxides,and Si−Graphite composites(SiGC),was conducted to characterize their overall performance in high-energy lithium-ion battery(LIB)design by incorporating conventional ***-Si was found to exhibit poor electrochemical performance,with severe volume expansion during *** suboxide provided excellent cycling stability in a full-cell evaluation with stable volume variation after 50 cycles,but had a large irreversible capacity and remarkable volume expansion during the first *** displayed a good initial Coulombic efficiency and the lowest volume change in the first cycle owing to the uniformly distributed nano-Si layer on graphite;however,its long-term cycling stability was relatively *** complement each disadvantage of Si suboxide and SiGC,a new combination of these Si-based anodes was suggested and a reasonable improvement in overall battery performance was successfully achieved.

关键词： silicon-based anode high-energy comparison lithium ion battery blended electrode

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Strengthening mechanisms analysis and tailoring of bimodal grain structures for enhanced strength in CoCrFeMnNi high-entropy alloys

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Rare Metals 2024年第8期43卷 3893-3903页

作者： Jaesoung Lee Seulgi kim Taehyun Kwon Young II kim Suyeon kim sung Ho Song Bin Lee Dongju Lee Department of Urban Energyand Environmental EngineeringChungbuk National UniversityCheongju 28644Republic of Korea Division of Advanced Materials Engineering Kongju National UniversityChungnam 330-717Republic of Korea Department of Advanced Materials Engineering for Information and Electronics Integrated-Education Institute for Frontier Science and Technology(BK21 Four)Kyung Hee UniversityYongin17104Republic of Korea

The CoCrFeMnNi high-entropy alloys(HEAs)with a(face-centered cubic) FCC structure has garnered considerable attention for its exceptional ductility and strain hardening ***,its yield strength is insufficient for structural *** this study,strengthening mechanisms in these HEAs were investigated to gain insight into the mechanical properties according to alloy powder ***,we present a novel approach to achieve both high strength and high ductility through the creation of a bimodal structure consisting of both coarse and fine grains via gas atomization and spark plasma sintering processes.A bimodally structured HEA prepared with a mass ratio of 2:8 between coarse particles(75-106 μm) and fine particles(≤25 μm)yielded optimal results,with a strength of 491.95 MPa and elongation of 19.64%.This strength value represents an~41% increase compared with the sample that displayed a fine single microstructure(347.08 MPa for yield strength).The strength enhancement was attributed to the prevention of plastic deformation initiation from the fine particles during *** innovative approach to the creation of HEAs with bimodal structures shows promise for various applications,such as structural components that require a combination of high strength and high ductility.

关键词： High-entropy alloys CrCoFeMoNi Bimodal Gas atomization Mechanical properties

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