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In-Vivo Anti-Hyperlipidemic Activity and Preliminary Phytochemical Screening of Canephora robusta

American Journal of Molecular Biology 2020年第1期10卷 44-50页

作者： Kishan Pavani Jankuti Anusha Govindula Rajkumar Jampala Mohammad Arif Pasha Aligeti Sravan Kumar sunayana soorammagari Chinnela Satish Mahankali Naga Ganesh Department of Pharmaceutics Vaageswari College of Pharmacy Karimnagar India Department of Pharmacology Vaageswari College of Pharmacy Karimnagar India Department of Pharmaceutical Chemistry Vaageswari College of Pharmacy Karimnagar India Department of Clinical Pharmacy Vaageswari College of Pharmacy Karimnagar India Department of Pharmaceutics Geethanjali College of Pharmacy Hyderabad India

The main aim of this study is to determine the anti-hyerlipidemic and anti-obesity activity of Canephora robusta in hyperlipidemia induced rats. Prepared coffee bean extract (GCE) was procured from the market which is unroasted and contains more quantity of caffeine and chlorogenic acid when compared to roasted coffee. Male albino Wister rats are fed with high fat diet (HFD) for weeks to induce hyperlipidemia in rats, which are divided into 4 groups with 4 animals in each group. Test GCBE was given in doses of 200 mg/kg and 400 mg/kg to III and IV groups which are fed with HFD for 30 days. Then blood samples were collected through retro-orbital sinus by capillaries and serum is separated for analysis. The result obtained from lipid profile which includes total cholesterol, triglycerides, very low density lipoproteins, and low density lipoproteins shows the decreased level when compared to the hyperlipidemic control. This shows the significant reduction of total body weight (p < 0.05) when given with dose of 200 mg/kg and 400 mg/kg. The present study suggests that GCBE has anti-obesity and anti-hyperlipidemic activity, where 400 mg/kg is more effective to reduce the total body weight and lipid levels when compared to 200 mg/kg. Further studies on this extract may lead to identify the possible mechanism of action and isolation of active principle from the same.

关键词： Anti-Hyerlipidemic Anti-Obesity Lipoproteins Hyperlipidemia

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Population Structure and Marker-Trait Association in Indigenous Aromatic Rice

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Rice science 2017年第3期24卷 145-154页

作者： Preeti Rekha TALUKDAR sunayana RATHI Khanin PATHAK Sanjay Kumar CHETIA Ramendra Nath SARMA Department of Plant Breeding and Genetics Assam Agricultural University Jorhat 785013 India Biochemistry & Agricultural Chemistry Assam Agricultural University Jorhat 785013 India Regional Agricultural Research Station Assam Agricutural University Jorhat 785013 India

Joha rice of Assam is a class of rice with aroma, good cooking qualities and excellent palatability, which are grown in different places of Assam with their adaptability to local situations. A total of 143 simple sequence repeat markers were used in this study to reveal sufficient genetic variations among the 40 Joha and 14 non-Joha rice genotypes. Polymorphism information content of these markers were from 0.17 to 0.88, where the average of 3.7 alleles were observed. The model-based population assignment and dendrogram analysis identified three distinct groups in the 54 rice accessions, which established a clear identity of Joha accessions from the Basmati accessions, indicating high diversity and strong population structure in Joha rice. Furthermore, a total of 29 significant marker-trait associations(P < 0.05) for 10 characters were detected. The QTLs related with yield and grain quality can be used effectively in crop improvement programs and for further fine mapping and validation of specific genes to develop gene-based perfect markers in rice breeding and for mining of better alleles of these genes in Joha rice collections.

关键词： Joha rice genetic diversity association mapping population structure quantitative trait locus aroma

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Variability in Grain Quality Characters of Upland Rice of Assam,India

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Rice science 2010年第4期17卷 330-333页

作者： sunayana RATHI Raj Narain Singh YADAV Ramendra Nath SARMA Centre for Studies in Biotechnology Dibrugarh University Department of Plant Breeding & Genetics Assam Agricultural University

The grain quality of 100 uplandlahu rice genotypes from Assam, India was assessed. The characteristics measured were grain length, grain width, cooked grain length, cooked grain width, grain elongation ratio after cooking, grain widening ratio after cooking, alkali spreading value, gel consistency, as well as starch, amylose, amylopectin and total soluble sugar contents. Considerable genetic variability among the 100 rice genotypes was observed for the characteristics under study. Analysis of vadance revealed highly significant differences among the genotypes for all the characteristics. The difference between genotypic coefficient of variation （GCV） and phenotypic coefficient of variation （PCV） was very small for all the characteristics analyzed. High GCV and PCV were found in the characteristic of total soluble sugar content followed by alkali spreading value. The high values for these traits signify the scope for selection to develop superior genotypes. High heritability was recorded in all the characteristics analyzed along with diverse genetic advance which varied from 9.71% to 52.83%. The characteristic of total soluble sugar content showed high heritability with high genetic advance, whereas alkali spreading value and grain widening ratio presented high hedtability with moderate genetic advance. This implies that these characteristics are mainly under additive gene effects and could be relied upon further selection based on phenotypic performance. The genotypic correlation coefficient was higher than the phenotypic correlation coefficient, indicating strong inherent associations among the characteristics studied.

关键词： upland rice alkali spreading value gel consistency genetic variability grain quality

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