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Comparative analysis and phylogenetic and evolutionary implications of mitogenomes of Chinese sinocyclocheilus cavefish(Cypriniformes:Cyprinidae)

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Zoological Research 2023年第4期44卷 779-781页

作者： Wan-Sheng jiang Jie li Hong-Mei Xiang Chao sun Jian-Bo chang Jun-xing Yang Hunan Engineering Laboratory for Chinese Giant Salamander’s Resource Protection and Comprehensive Utilization and Key Laboratory of Hunan Forest Products and Chemical Industry EngineeringJishou UniversityZhangjiajieHunan 427000China College of Biology and Environmental Sciences Jishou UniversityJishouHunan 416000China Hubei Fisheries Science Research Institute WuhanHubei 430071China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China State Key Laboratory of Water Resources Engineering and Management School of Water Resources and Hydropower EngineeringWuhan UniversityWuhanHubei 430072China

DEAR EDITOR,Cavefish are of considerable interest due to the remarkable morphological changes that occurred during their adaptation to cave or subterranean river *** changes are thought to involve trade-offs in metabolic requirements and energy *** play a vital role in oxygen use and energy metabolism;thus,mitochondrial genes are likely to have experienced specific selective pressures during cavefish *** this study,we assembled and annotated the mitogenome of sinocyclocheilus sanxiaensis,a typical cavefish species in *** this sequence and other available mitogenomes from the NCBI database,we reconstructed an updated phylogeny of sinocyclocheilus based on 29 *** species groups were revealed,with the recently erected *** species group *** determine whether divergent selective pressures have acted on the protein-coding genes(PCGs)of the 29 sinocyclocheilus species exhibiting different morphological features(including eye shape and body color),the fish were classified into three groups according to their cave-related *** branch model test revealed higher nonsynonymous/synonymous substitution ratios(ω)in the cave-dwelling groups,potentially resulting from a balance between adaptive selection and relaxed functional *** analysis using site and branch-site models identified 24 highly credible positive selection sites located on nine PCGs,with most sites also showing radical changes in amino acid *** study sheds light on the phylogeny and mitogenomic evolution of sinocyclocheilus cavefish,highlighting their successful adaptation and survival in subterranean environments.

关键词： fish phylogeny habitat

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肌电生物反馈疗法与脊髓损伤患者运动功能的恢复

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中国组织工程研究 2025年第14期29卷 3002-3010页

作者：梁佳佳孙姣姣刘文洁邢政李奇李庆雯褚晓蕾天津体育学院运动健康学院天津市301617 天津市天津医院天津市300211

背景:肌电生物反馈技术具有非侵入性、实时反馈、个性化治疗和促进神经可塑性等独特优势,加速了脊髓损伤患者的功能恢复。目的:综述肌电生物反馈联合运动疗法、外骨骼机器人以及虚拟现实技术在脊髓损伤运动功能恢复中的应用现状及治疗... 详细信息

背景:肌电生物反馈技术具有非侵入性、实时反馈、个性化治疗和促进神经可塑性等独特优势,加速了脊髓损伤患者的功能恢复。目的:综述肌电生物反馈联合运动疗法、外骨骼机器人以及虚拟现实技术在脊髓损伤运动功能恢复中的应用现状及治疗效果。方法:应用计算机检索中国知网、PubMed数据库建库至2024年5月期间的相关文献,英文检索词为“Spinal cord injury,EMG biofeedback,physical therapy,robotic exoskeleton,VR,motor function,exercise”,中文检索词为“脊髓损伤,肌电生物反馈,运动疗法,外骨骼机器人,虚拟现实,运动功能,运动”,最终纳入71篇文献进行综述。结果与结论:肌电生物反馈疗法促进脊髓损伤康复的机制包括促进神经可塑性变化、增强神经肌肉连接和改善运动模式。肌电生物反馈作为新兴的技术手段,通常与运动疗法、外骨骼机器人以及虚拟现实联合用于脊髓损伤后运动功能的恢复,综合治疗效果得到明显提升。然而目前仍存在一些问题和挑战,例如缺乏机制的详细解析、缺少为结合疗效提供有力证据的大规模试验、技术适应性有限等。未来的研究可以集中在这些方面:提高肌电生物反馈的个性化和精准性;可以探索将肌电生物反馈与更先进的技术或工学设备相结合开发新型康复设备,扩大应用领域;将肌电生物反馈应用到步态训练系统、呼吸训练系统以及四肢联动康复系统中,提高反馈的准确性和个性化治疗方案的效果,同时提高设备的易用性和舒适性。

关键词：脊髓损伤肌电生物反馈运动疗法外骨骼机器人联合治疗运动康复

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Ferric ion-triggered surface oxidation of galena for efficient chalcopyrite-galena separation

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International Journal of Minerals,Metallurgy and Materials 2024年第2期31卷 261-267页

作者： qiancheng Zhang limin Zhang Feng jiang Honghu Tang li Wang wei sun School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Engineering Research Center of Ministry of Education for Carbon Emission Reduction in Metal Resource Exploitation and Utilization Changsha 410083China School of Resource and Environment Hunan University of Technology and BusinessChangsha 410083China

The efficient separation of chalcopyrite(CuFeS2)and galena(PbS)is essential for optimal resource ***,find-ing a selective depressant that is environmentally friendly and cost effective remains a *** various techniques,such as mi-croflotation tests,Fourier transform infrared spectroscopy,scanning electron microscopy(SEM)observation,X-ray photoelectron spec-troscopy(XPS),and Raman spectroscopy measurements,this study explored the use of ferric ions(Fe^(3+))as a selective depressant for *** results of flotation tests revealed the impressive selective inhibition capabilities of Fe^(3+)when used *** analysis showed that Fe^(3+)significantly reduced the adsorption of isopropyl ethyl thionocarbamate(IPETC)on the galena surface while having a minimal impact on *** analysis using SEM,XPS,and Raman spectra revealed that Fe^(3+)can oxidize lead sulfide to form compact lead sulfate nanoparticles on the galena surface,effectively depressing IPETC adsorption and increasing surface *** findings provide a promising solution for the efficient and environmentally responsible separation of chalcopyrite and galena.

关键词： galena chalcopyrite ferric ions flotation separation surface oxidation

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Minimizing Carbon Content with Three-in-One Functionalized Nano Conductive Ceramics:Toward More Practical and Safer S Cathodes of li-S Cells

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Energy & Environmental Materials 2023年第3期6卷 31-39页

作者： Ning li chang sun Jianhui Zhu Shun li Yanlong Wang Maowen Xu changming li Jian jiang School of Materials and Energy and Chongqing Key Lab for Advanced Materials and Clean Energies of TechnologiesSouthwest UniversityChongqing 400715China School of Physical Science and Technology Southwest UniversityChongqing 400715China Key Laboratory of Chemical Lasers Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China College of Chemistry and Chemical Engineering Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Provinceand Key Laboratory of Functional Materials and Photoelectrochemistry of HaikouHainan Normal UniversityHaikou 571158China

Using porous carbon hosts in cathodes of li-S cells can disperse S actives and offset their poor electrical ***,such reservoirs would in turn absorb excess electrolyte solvents to S-unfilled regions,causing the electrolyte overconsumption,specific energy decline,and even safety hazards for battery *** build better cathodes,we propose to substitute carbons by In-doped SnO_(2)(ITO)nano ceramics that own three-in-one functionalities:1)using conductive ITO enables minimizing the total carbon content to an extremely low mass ratio(~3%)in cathodes,elevating the electrode tap density and averting the electrolyte overuse;2)polar ITO nanoclusters can serve as robust anchors toward li polysulfide(liPS)by electrostatic adsorption or chemical bond interactions;3)they offer catalysis centers for liquid–solid phase conversions of S-based ***,such ceramics are intrinsically nonflammable,preventing S cathodes away from thermal runaway or *** merits entail our configured cathodes with high tap density(1.54 g cm^(−3)),less electrolyte usage,good security for flame retardance,and decent li-storage *** lean and liNO_(3)-free electrolyte,packed full cells exhibit excellent redox kinetics,suppressed liPS shuttling,and excellent *** may trigger great research enthusiasm in rational design of low-carbon and safer S cathodes.

关键词： flame retardance li-S cells minimized carbon ratio nano conductive ceramics three-in-one functionality

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Influences of petroleum on accumulation of copper and cadmium in the polychaete Nereis diversicolor

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Journal of Environmental Sciences 2006年第1期18卷 109-114页

作者： sun Fu-hong ZHOU qi-xing ZHANG qian-ru Key Laboratory of Terrestrial Ecological Process Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China College of Environmental Sciences and Engineering Nankai University Tianjin 300071 China

Using the exposure simulation experiment, the action of petroleum affecting the accumulation of the trace metals including copper （Cu） and cadmium （Cd） in littoral polychaete Nereis diversicolor collected from the Shuangtaizi Estuary in liaoning Province, China was examined. The results showed that there was a markedly non-linear relationship between the accumulation of Cu in worms and the experimental concentration of Cu in exposure solutions when the concentration of petroleum remained at 0, 100, and 220 μl/L, respectively. However, significantly non-linear relationship for worms exposed to Cd was observed only when the concentration of added petroleum was 0 and 220 μl/L. The accumulation of Cu in worms did not differ significantly among the three different levels of petroleum concentrations combined with various concentrations of Cu. So was the accumulation of Cd in worms （p〉0.05）. However, the addition of petroleum in exposure solutions brought about an increase in the accumulation of Cu in Nereis diversicolor, in comparison with single Cu pollution. On the other hand, when the concentration of added petroleum remained at 100 μl/L, the accumulation of Cd in worms was lower than that in worms exposed to various concentrations of only cadmium. However, the worms exposed to Cd and petroleum 220 μl/L did not show obvious and identical increase in the accumulation of Cd, compared with single Cd exposure. The accumulation of both Cu and Cd in worms did not increase significantly with the increases in concentrations of Cu or Cd in exposure solutions combined with petroleum （0, 100, and 220μl/L） under the experimental conditions. Although Nereis diversicolor is exposed to very high Cu and Cd in exposure solutions, accumulation and detoxification mechanisms are sufficient to cope with the extra metal influx in order to survive.

关键词： Nereis diversicolor copper cadmium petroleum combined pollution

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Synthesis of novel thiol-functionalized mesoporous silica nanorods and their sorbent properties on heavy metals

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Frontiers of Materials Science 2012年第3期6卷 278-282页

作者： Xi CHEN qiang CAI lin-Hao sun wei ZHANG xing-Yu jiang Department of Materials Science and Engineering Tsinghua UniversityBeijing 100084China National Center for Nanoscience and Technology Beijing 100190China

Novel thiol-functionalized mesoporous silica nanorods （MSNRs） were synthesized through a base co-condensation method, in which two organoalkoxysilanes, tetraethoxylsilane （TEOS） and bis[3-（triethoxysilyl）propyl]tetrasulfide （TESPT）, were used as silica precursors simultaneously. TESPT was firstly used for both morphology control and inner surface functionalization of mesoporous silica hybrid materials. The micro- structures as well as porous character of the MSNRs were characterized by means of SEM, XRD, TEM and N2 sorption measurements. Infrared spectrum analysis and heavy metal ions （Ag＋ and Cd2＋） adsorption measurements were carried out to confirm the functionalized framework of MSNRs.

关键词： nanocomposite porous material morphology control inner surfacefunctionalization sorbent

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Adaptability of microbial inoculators and their contribution to degradation of mineral oil and PAHs

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Journal of Environmental Sciences 2006年第2期18卷 310-317页

作者： SONG Xue-ying SONG Yu-fang sun Tie-heng ZHOU qi-xing ZHANG wei ZHANG Lei Key Laboratory of Terrestrial Ecological Process Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China Graduate School of Chinese Academy of Sciences Beijing 100039 China Key Laboratory of Environmental Engineering Shenyang University Shenyang 100041 China Institute of Microbiology Chinese Academy of Sciences Beijing 100081 China

Five dominant bacteria strains（A cetobacter sp., Alcdigenes sp., Micrococcus sp., Arthrobacter sp. and Bacillus sp.） and five fungi strains （Cephalosporium sp. Ⅰ, Cephalosporlum sp. Ⅱ, Aspergillus sp. Ⅰ, Aspergillus sp. Ⅱ and Fusarium sp.） isolated from petroleum-contaminated soil were used to assess the potential capability of mineral oil and PAH enhanced degradation separately and jointly using the batch liquid medium cultivation with diesel oil spiked at 1000 mg/L. The experiment was performed on a reciprocal shaker in the darkness at 25℃ to 30℃ for 100 d. The dynamic variation in the activity of microbial inoculators in each treatment and the degradation of the target pollutants during the period of experiment were monitored. Results showed a more rapid biodegradation of mineral oil and PAHs at the beginning of the experiment （about 20 d） by dominant bacteria, fungi and their mixture than that of the indigenous microorganisms, however, thereafter an opposite trcnd was exhibited that the removal ratio by indigenous microorganisms was superior to any other dominant treatments and the tendency lasted till the end of the experiment, indicating the limited competitive capability of dominant microorganisms to degrade the contaminants, and the natural selection of indigenous microorganisms for use in the removal of the contaminants. At the end of the experiment, the removal ratio of mineral oil ranged from 56.8 % to 79.2 % and PAHs ranged from 96.8 % to 99.1% in each treatment by microbial inoculators,

关键词： dominant microorganism indigenous microorganism mineral oil PAHs biodegradation

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Response to weaning and dietary L-glutamine supplementation:metabolomic analysis in piglets by gas chromatography/mass spectrometry

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第7期13卷 567-578页

作者： Ying-ping XIAO Tian-xing WU qi-hua HONG jiang-ming sun An-guo CHEN Cai-mei YANG Xiao-yan li College of Animal Sciences Zhejiang UniversityHangzhou 310058China Key Laboratory for Molecular Design and Nutrition EngineeringNingbo Institute of TechnologyZhejiang UniversityNingbo 315100China Department of Chemistry Tongji UniversityShanghai 200092China

A novel metabolomic method based on gas chromatography/mass spectrometry （GC-MS） was applied to determine the metabolites in the serum of piglets in response to weaning and dietary L-glutamine （Gin） supplementation. Thirty-six 21-d-old piglets were randomly assigned into three groups. One group continued to suckle from the sows （suckling group）, whereas the other two groups were weaned and their diets were supplemented with 1% （w/w） Gin or isonitrogenous L-alanine, respectively, representing Gin group or control group. Serum samples were collected to characterize metabolites after a 7-d treatment. Results showed that twenty metabolites were down-regulated significantly （P〈0.05） in control piglets compared with suckling ones. These data demonstrated that early weaning causes a wide range of metabolic changes across arginine and proline metabolism, aminosugar and nucleotide metabolism, galactose metabolism, glycerophospholipid metabolism, biosynthesis of unsaturated fatty acid, and fatty acid metabolism. Dietary Gin supplementation increased the levels of creatinine, D-xylose, 2-hydroxybutyric acid, palmitelaidic acid, and a-L-galactofuranose （P〈0.05） in eady weaned piglets, and were involved in the arginine and proline metabolism, carbohydrate metabolism, and fatty acid metabolism. A leave-one-out cross-validation of random forest analysis indicated that creatinine was the most important metabolite among the three groups. Notably, the concentration of creatinine in control piglets was decreased （P=0.00001） compared to the suckling piglets, and increased （P=0.0003） in Gin-supplemented piglets. A correlation network for weaned and suckling piglets revealed that early weaning changed the metabolic pathways, leading to the abnormality of carbohydrate metabolism, amino acid metabolism, and lipid metabolism, which could be partially improved by dietary Gin supplementation. These findings provide fresh insight into the complex metabolic changes in response to early weaning

关键词： Piglet Metabolomics Weaning Glutamine Gas chromatography/mass spectrometry

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Metal-free C3-alkoxycarbonylation of quinoxalin-2(1H)-ones with carbazates as ecofriendly ester sources

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Science China Chemistry 2019年第4期62卷 460-464页

作者： Long-Yong Xie Sha Peng Tai-Gang Fan Yan-Fang liu Meng sun li-lin jiang xing-xing Wang Zhong Cao wei-Min He Department of Chemistry Hunan University of Science and Engineering Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education Hunan University of Science and Technology Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation Changsha University of Science and Technology

Quinoxaline-3-carboxylates and analogues are prevalent key structural motifs in bioactive natural products and synthetic ***, the practical protocol for preparation of these motifs from simple raw materials under mild conditions remains rare. In this article, we report a facile protocol for the efficient preparation of various quinoxaline-3-carbonyl compounds(30 examples,63%–92%) through oxidation coupling of quinoxalin-2(1H)-ones with readily available carbazates(or acyl hydrazines) in the presence of K_2S_2O_8 as an oxidant in metal-and base-free conditions. When tert-butyl carbazate was used as the coupling reagent,the decarboxylation product 3-(tert-butyl)-1-methylquinoxalin-2(1H)-one was obtained. The application of this process into a gram-scale synthesis can be easily accomplished. Mechanistic investigations reveal that the functionalization of quinoxalin-2(1H)-ones via a free-radical pathway.

关键词： quinoxalin-2(1H)-ones alkoxycarbonylation carbazates synthetic methods metal-free

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Novel supramolecular organocatalysts of hydroxyprolinamide based on calix[4]arene scaffold for the enantioselective Biginelli reaction

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Science China Chemistry 2011年第11期54卷 1726-1734页

作者： li ZhengYi xing HuaiJie HUANG Guoli sun Xiaoqiang jiang Juli WANG LeYong Key Laboratory of Fine Petrochemical Engineering School of Petrochemical Engineering Changzhou University Changzhou 213164 China Institute of Chemical Biology and Drug Innovation School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China

A series of novel supramolecular organocatalysts of hydroxyprolinamide based on the upper rim of calix[4]arene scaffold have been developed to catalyze enantioselective multi-component Biginelli reaction. Under the optimal conditions, the reactions occurred with moderate-to-excellent enantioselectivities (up to 98% ee). A plausible transition state constructed by the supra- molecular interaction of hydrogen bond and cation-π between catalysts and substrates has been proposed.

关键词： supramolecular organocatalyst calixarene hydroxyprolinamide enantioselective Biginelli reaction

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