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Brainbow示踪小鼠心肌细胞增殖中他莫昔芬最适标记剂量的探究

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中国病理生理杂志 2024年第6期40卷 1147-1152页

作者：高洋洋高佳孙萌王睿山西医科大学第一临床医学院山西太原0300001 山西医科大学第一医院山西太原0300001

目的:运用Brainbow 2.1小鼠进行心肌细胞谱系追踪,探讨用于心肌细胞增殖标记的最适他莫昔芬剂量。方法:采用4周龄的Brainbowfl/+;Myh6-MerCreMe+小鼠,通过腹腔注射的方式给予3个不同剂量(3、9和27 mg/kg)的他莫昔芬诱导Cre重组酶表达,... 详细信息

目的:运用Brainbow 2.1小鼠进行心肌细胞谱系追踪,探讨用于心肌细胞增殖标记的最适他莫昔芬剂量。方法:采用4周龄的Brainbowfl/+;Myh6-MerCreMe+小鼠,通过腹腔注射的方式给予3个不同剂量(3、9和27 mg/kg)的他莫昔芬诱导Cre重组酶表达,使心肌细胞标上特定的颜色,4周后取材染色,共聚焦显微镜下定量不同剂量的他莫昔芬标记的心肌细胞数量,以及被标记的细胞中单个细胞、临近的2个细胞簇和临近的3个及以上细胞簇的比例。选择合适剂量(9 mg/kg)的他莫昔芬标记Brainbow小鼠,对比假手术组与心肌梗死(mi)模型组心肌细胞增殖情况。结果:(1)注射9 mg/kg的他莫昔芬后标记的单个细胞比例为99.13%±0.03%,临近的2个细胞簇比例为0.82%±0.09%,3个及以上细胞簇比例为0.05%±0.01%。3 mg/kg组标记总数较少;27 mg/kg组出现明显克隆扩增富集。(2)用9 mg/kg的他莫昔芬标记心肌细胞后,mi小鼠2个细胞簇和3个及以上细胞簇标记比例较假手术组均显著升高(Pmi后心肌细胞增殖比例增加。结论:9 mg/kg他莫昔芬标记的临近心肌细胞簇的比例更接近生理状态下的心肌细胞增殖比例,因此9 mg/kg为示踪心肌细胞增殖的最适他莫昔芬剂量。

关键词： Brainbow小鼠心肌细胞细胞增殖他莫昔芬荧光标记

The HIF-1α/PLOD2 axis integrates extracellular matrix organization and cell metabolism leading to aberrant musculoskeletal repair

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Bone Research 2024年第2期12卷 289-301页

作者： Heeseog Kang Amy L.Strong Yuxiao sun Lei Guo Conan Juan Alec C.Bancroft Ji Hae Choi Chase A.Pagani Aysel A.Fernandes michael Woodard Juhoon Lee Sowmya Ramesh Aaron W.James David Hudson Kevin N.Dalby Lin Xu Robert J.Tower Benjamin Levi Center for Organogenesis Regeneration and TraumaDepartment of SurgeryUniversity of Texas SouthwesternDallasTX 75390USA Section of Plastic Surgery Department of SurgeryUniversity of MichiganAnn ArborMI 48109USA Quantitative Biomedical Research Center Peter O’Donnell Jr.School of Public HealthUniversity of Texas SouthwesternDallasTX 75390USA Department of Orthopedics and Sports Medicine University of WashingtonSeattleWA 98195USA Division of Chemical Biology and Medicinal Chemistry College of PharmacyUniversity of Texas at AustinAustinTX 78712USA Department of Pathology Johns Hopkins UniversityBaltimoreMD 21218USA

While hypoxic signaling has been shown to play a role in many cellular processes,its role in metabolism-linked extracellular matrix(ECM)organization and downstream processes of cell fate after musculoskeletal injury remains to be ***(HO)is a debilitating condition where abnormal bone formation occurs within extra-skeletal *** andhypoxia-inducible factor 1α(HIF-1α)activation have been shown to promote ***,the underlying molecular mechanisms bywhich the HIF-1αpathway in mesenchymal progenitor cells(MPCs)contributes to pathologic bone formation remain to ***,we used a proven mouse injury-induced HO model to investigate the role of HIF-1αon aberrant cell ***-cell RNA sequencing(scRNA-seq)and spatial transcriptomics analyses of the HO site,we found that collagen ECM organizationis the most highly up-regulated biological process in *** mesenchymal cell-specific deletion of Hif1α(Hoxa11-CreER^(T2);Hif1a^(fl/fl))significantly mitigated HO in ***-seq analysis of these Hoxa11-CreER^(T2);Hif1a^(fl/fl)mice identified the PLOD2/LOXpathway for collagen cross-linking as downstream of the HIF-1αregulation of ***,our scRNA-seq data and mechanisticstudies further uncovered that glucose metabolism in MPCs is most highly impacted by HIF-1α*** a translational aspect,a pan-LOX inhibitor significantly decreased HO.A newly screened compound revealed that the inhibition of PLOD2 activity in MPCssignificantly decreased osteogenic differentiation and glycolytic *** suggests that the HIF-1α/PLOD2/LOX axis linked tometabolism regulates HO-forming MPC *** results suggest that the HIF-1α/PLOD2/LOX pathway represents a promisingstrategy to mitigate HO formation.

关键词： metabolism skeletal aberrant

人类免疫缺陷病毒高值假阳性样本2例分析报告

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实用医技杂志 2024年第2期31卷 87-89页

作者：米慧孙秀明吴虹张玉洁李敏杨伟黄成毅刘小溪孙磊长治市人民医院检验科长治046000 长治市人民医院感染科长治046000 迈克生物股份有限公司研发中心成都610000

目的对山西省长治市人民医院检验科2022年化学发光法筛选出的人类免疫缺陷病毒(HIV)高值假阳性患者2例血清样本进行分析报告。方法2份标本均采用直接化学发光法(CmiA法)复检,对其进行干扰原因分析,使用类风湿因子(RF)测定试剂盒测定RF,... 详细信息

目的对山西省长治市人民医院检验科2022年化学发光法筛选出的人类免疫缺陷病毒(HIV)高值假阳性患者2例血清样本进行分析报告。方法2份标本均采用直接化学发光法(CmiA法)复检,对其进行干扰原因分析,使用类风湿因子(RF)测定试剂盒测定RF,用2-巯基乙醇、异嗜性抗体封闭管、6%的聚乙二醇和12%的聚乙二醇处理样本后用HIV抗原抗体检测试剂盒复检。结果2份样本CmiA法结果为有反应,经过处理后结果降为阴性。结论样本1中干扰物质可能同时与P24-Fab端和P24-Fc端产生非特异性吸附,导致检测结果为假阳性,而2-巯基乙醇可以破坏干扰物质,6%聚乙二醇(PEG6000)和12%PEG6000可以沉淀干扰物质,因此处理后结果将为阴性;样本2可能是由于异嗜性抗体吸附P24-Fc端、HIV-Ⅱ型抗原以及磁珠产生的假阳性,根据PEG6000处理后的结果推测异嗜性抗体存在IgM型和IgG型。

关键词： HIV 发光测定法假阳性反应

Enrichment characteristics and exploration directions of deep shale gas of Ordovician-Silurian in the Sichuan Basin and its surrounding areas,China

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Petroleum Exploration and Development 2022年第4期49卷 744-757页

作者： NIE Haikuan LI Pei DANG Wei DING Jianghui sun Chuanxiang LIU mi WANG Jin DU Wei ZHANG Peixian LI Donghui SU Haikun State Key Laboratory of Shale Oil and Gas Enrichment Mechanisms and Effective Development Beijing 102206China Sinopec Petroleum Exploration and Production Research Institute Beijing 102206China School of Earth Sciences and Engineering Xi’an Shiyou UniversityXi’an 710300 CNPC Engineering Technology R&D Company limited Beijing 102206China Research Institute of Petroleum Exploration and Development Sinopec Jianghan Oilfield CompanyWuhan 430223China Sinopec East China Company Nanjing 210019China School of Energy Resources China University of GeosciencesBeijing 100083China

The enrichment characteristics of deep shale gas in the Ordovician Wufeng-Silurian Longmaxi formations in the Sichuan Basin and its surrounding areas are investigated through experiments under high temperature and high pressure,including petrophysical properties analyses,triaxial stress test and isothermal adsorption of methane experiment.(1)The deep shale reservoirs drop significantly in porosity and permeability compared with shallower shale reservoirs,and contain mainly free gas.(2)With higher deviatoric stress and axial strain,the deep shale reservoirs have higher difficulty fracturing.(3)Affected by structural location and morphology,fracture characteristics,geofluid activity stages and intensity,deep shale gas reservoirs have more complicated preservation conditions.(4)To achieve the commercial development of deep shale gas reservoirs,deepening geological understanding is the basis,and exploring reservoir simulation technology befitting the geological features is the key.(5)The siliceous shale and limestone-bearing siliceous shale in the Metabolograptus persculptus-Parakidograptus acuminatus zones(LM1-LM3 graptolite zones)are the high-production intervals for deep shale gas and the most favorable landing targets for horizontal *** water areas such as Jiaoshiba,Wulong,Luzhou and Changning with deep shale reservoirs over 10 m thickness are the most favorable areas for deep shale gas *** is recommended to carry out exploration and development practice in deep-water shale gas areas deposited deep with burial depth no more than 5000 m where the geological structure is simple and the shale thickness in the LM1-LM3 graptolite zone is greater than 10 *** is better to increase the lateral length of horizontal wells,and apply techniques including high intensity of perforations,large volume of proppant,far-field and near-wellbore diversions to maximize the stimulated deep reservoir volume.

关键词： deep shale gas enrichment conditions reservoir characteristics exploration direction Ordovician Wufeng Formation Silurian Longmaxi Formation Sichuan Basin

动态心电图联合血清IMA、GRP78、miR-29a诊断冠心病心肌缺血的临床价值

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临床和实验医学杂志 2024年第5期23卷 548-551页

作者：王茹孙瑞孙娜宿迁市第一人民医院心血管内科江苏宿迁223800

目的前瞻性研究动态心电图(DCG)联合血清缺血修饰白蛋白(IMA)、葡萄糖调节蛋白78(GRP78)、微小RNA-29a(miR-29a)诊断冠心病心肌缺血的临床价值。方法回顾性选择2020年7月至2022年8月于宿迁市第一人民医院就诊的冠心病心肌缺血患者100例... 详细信息

目的前瞻性研究动态心电图(DCG)联合血清缺血修饰白蛋白(IMA)、葡萄糖调节蛋白78(GRP78)、微小RNA-29a(miR-29a)诊断冠心病心肌缺血的临床价值。方法回顾性选择2020年7月至2022年8月于宿迁市第一人民医院就诊的冠心病心肌缺血患者100例,按照冠脉病变支分为单支组(n=67)、多支组(n=33),两组均行DCG联合血清IMA、GRP78、miR-29a检查。观察两组DCG特征;血清IMA、GRP78、miR-29a水平。以冠脉造影诊断结果为金标准,评价DCG及血清IMA、GRP78、miR-29a单独、联合诊断冠心病心肌缺血的诊断效能。分析冠心病心肌缺血患者DCG特征及血清IMA、GRP78、miR-29a水平与冠心病心肌缺血的病变程度相关性。结果单支组ST段压低幅度、ST段压低持续时间、心肌缺血发作频次分别为(1.41±0.16)mm、(7.82±0.80)min、(2.04±0.22)次、均小于多支组[(3.42±0.36)mm]、(17.13±1.93)min、(4.32±0.45)次],差异均有统计学意义(PmiR-29a水平分别为(1.32±0.12)ng/mL、1.84±0.21,均低于单支组[(2.26±0.24)ng/mL、3.95±0.42],差异均有统计学意义(PmiR-29a四者联合诊断冠心病心肌缺血的敏感度、特异度、阳性预测值及阴性预测值均高于四者单独、两两及三三联合诊断(PmiR-29a水平与冠心病心肌缺血不同病变程度呈正相关(r=0.812、0.737、0.713、0.594、0.686,PmiR-29a可提高对冠心病心肌缺血的诊断效能。

关键词：动态心电图 IMA GRP78 miR-29a 冠心病心肌缺血

Impact of guideline adherence on the prognosis of Barcelona clinic liver cancer stage B hepatocellular carcinoma

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World Journal of Gastroenterology 2023年第47期29卷 6122-6137页

作者： Ji Eun Han Hyo Jung Cho Jae Youn Cheong sun Gyo lim min Jae Yang Choong-Kyun Noh Gil Ho Lee Soon sun Kim Department of Gastroenterology Ajou University School of MedicineSuwon 16499Gyeonggi-doSouth Korea

BACKGROUND Patients with Barcelona clinic liver cancer(BCLC)stage B hepatocellular carcinoma(HCC)are considerably heterogeneous in terms of tumor burden,liver function,and performance *** improve the poor survival outcomes of these patients,treatment approaches other than transarterial chemoembolization(TACE),which is recommended by HCC guidelines,have been adopted in realworld clinical *** hypothesize that this non-adherence to treatment guidelines,particularly with respect to the use of liver resection,improves survival in patients with stage B *** To assess guideline adherence in South Korean patients with stage B HCC and study its impact on *** A retrospective analysis was conducted using data from 2008 to 2016 obtained from the Korea Central Cancer *** with stage B HCC were categorized into three treatment groups,guideline-adherent,upward,and downward,based on HCC guidelines recommended by the Asian Pacific Association for the Study of the Liver(APASL),the European Association for the Study of the Liver(EASL),and the American Association for the Study of Liver Diseases(AASLD).The primary outcome was HCC-related deaths;tumor recurrence served as the secondary *** among the groups was compared using the Kaplan-Meier method and the log-rank *** of survival outcomes were identified using multivariable Cox regression *** In South Korea, over the study period from 2008 to 2016, a notable trend was observed in adherence to HCCguidelines. Adherence to the EASL guidelines started relatively high, ranging from 77% to 80% between 2008 and2012, but it gradually declined to 58.8% to 71.6% from 2013 to 2016. Adherence to the AASLD guidelines began at71.7% to 75.9% from 2008 to 2010, and then it fluctuated between 49.2% and 73.8% from 2011 to 2016. In contrast,adherence to the APASL guidelines remained consistently high, staying within the range of 90.14% to 94.5%throughout the entire study per

关键词： Hepatocellular carcinoma Barcelona clinic liver cancer stage B Guideline adherence Liver neoplasms Transarterial chemoembolization Liver resection

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烟台市尘肺病患者生存质量及影响因素分析

职业与健康 2024年第1期40卷 14-17,23页

作者：米兆娟陈超孙东亮王磊刘薇阎西革烟台市疾病预防控制中心职业卫生科山东烟台264003 烟台市芝罘区疾病预防控制中心职业卫生科山东烟台264001 烟台市蓬莱区疾病预防控制中心职业卫生科山东烟台265600

目的了解烟台市尘肺病患者生存质量现状及影响因素,为制定提升尘肺病患者生存质量的措施提供参考依据。方法在1949—2020年烟台市诊断的职业性尘肺病现患病例数据库中按病程分层抽取300例为病例组,同性别、年龄相差不超过3岁的普通人... 详细信息

目的了解烟台市尘肺病患者生存质量现状及影响因素,为制定提升尘肺病患者生存质量的措施提供参考依据。方法在1949—2020年烟台市诊断的职业性尘肺病现患病例数据库中按病程分层抽取300例为病例组,同性别、年龄相差不超过3岁的普通人群600例为对照组。使用生存质量测定表简表(World Health Organization quality of life-bref,WHOQOL-BREF)和St George’s呼吸问卷(St George’s respiratory questionnaire,SGRQ)综合评价研究对象的生存质量,采用多元线性回归分析尘肺病患者生存质量的影响因素。结果病例组WHOQOL-BREF总分[M(P_(25),P_(75))]为224.20(199.96,246.60)分,显著低于对照组(P<0.05);SGRQ总分[M(P_(25),P_(75))]为137.30(109.70,186.50)分,显著高于对照组(P<0.05)。尘肺病患者生存质量的负向影响因素为尘肺合并症数量、其他慢性病数量;正向影响因素为集中供暖、个人月收入和睡眠时间。结论烟台市尘肺病患者的生存质量明显低于普通人群。积极预防尘肺并发症和其他慢性病的发生,改善患者采暖方式、收入水平和睡眠状况可提高尘肺病患者的生存质量。

关键词：尘肺病生存质量生存质量测定表简表 St George’s呼吸问卷影响因素

某油气终端处理厂水资源消耗分析及节水减排措施

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化工管理 2024年第3期 56-60页

作者：周开浪凌宇丁勇超米占虎谢进宜孙诗洋中海石油(中国)有限公司湛江分公司广东湛江524057

随着海上油田群滚动开发以及当地政府关于加强水资源开发利用和保护方案的实施,南海西部某油气终端处理厂生产过程中水资源供需矛盾日趋凸显。通过对终端水系统基本情况、用水结构以及存在的问题进行分析,从工艺优化、设备更新换型、输... 详细信息

随着海上油田群滚动开发以及当地政府关于加强水资源开发利用和保护方案的实施,南海西部某油气终端处理厂生产过程中水资源供需矛盾日趋凸显。通过对终端水系统基本情况、用水结构以及存在的问题进行分析,从工艺优化、设备更新换型、输水管道检测、非常规水资源利用以及加强综合管理五个方面出发,提出并针对实施相应节水减排措施,实现了生产用水自给自足,保障了正常稳定清洁生产,并为相关企业节水减排提供了借鉴和依据。

关键词：水资源用水结构节水减排清洁生产

Angular and planar transport properties of antiferromagnetic V_(5)S_(8)

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Chinese Physics B 2024年第2期33卷 66-71页

作者：吴晓凯王彬吴德桐陈博文弭孟娟王以林沈冰 Center for Neutron Science and Technology and School of Physics Guangdong Provincial Key Laboratory of Magnetoelectric Physics and Devicesand Key Laboratory of Optoelectronic Materials and TechnologiesSun Yat-Sen UniversityGuangzhou 510275China School of Integrated Circuits Shandong Technology Center of Nanodevices and IntegrationState Key Laboratory of Crystal MaterialsShandong UniversityJinan 250100China

Systemically angular and planar transport investigations are performed in layered antiferromagnetic(AF)V_(5)S_(8).In this AF system,obvious anomalous Hall effect(AHE)is observed with a large Hall angle of 0.1 compared to that in ferromagnetic(FM)*** can persist to the temperatures above AF transition and exhibit strong angular field *** phase diagram reveals various magnetic states by rotating the applied *** analyzing the anisotropic transport behavior,magnon contributions are revealed and exhibit obvious angular dependence with a spin-flop vanishing *** observed prominent planar Hall effect and anisotropic magnetoresisitivity exhibit two-fold systematical angular dependent *** behaviors are attributed to the scattering from spin–orbital coupling instead of nontrivial topological *** results reveal anisotropic interactions of magnetism and electron in V5S8,suggesting potential opportunities for the AF spintronic sensor and devices.

关键词： antiferromagnetism planar Hall effect magnetic and topological properties