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Genome-wide SNP markers provided insights into the reproductive strategy and genetic diversity of the green tide causative species Ulva prolifera in China

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Journal of Oceanology and Limnology 2024年第2期42卷 533-543页

作者： Song sun Weiwei MA Nan WANG Song FENG Yan sun CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Laoshan LaboratoryQingdao 266237China Jiaozhou Bay Marine Ecosystem Research Station Chinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China

Ulva prolifera is the causative species of the annually occurring large-scale green tides in China since *** specific biological features on reproductivity strategies,as well as intra-species genetic diversity,are still largely unknown,especially at the genome level,despite their importance in understanding the formation and outbreak of massive green *** the present study,the restriction site-associated DNA genotyping approach(2b-RAD)was adopted to identify the genome-wide single-nucleotide polymorphisms(SNPs)of 54 individual thalli including samples collected from Subei Shoal in 2019 and Qingdao coast from 2019 to *** genotype results revealed that most of the thalli in 2019 and 2020 were haploid gametophytes,while only half of the thalli were gametophytes in 2021,indicating flexibility in the reproductive strategies for the formation of the green tides among different years and the dominance of asexual and vegetative reproductive mode for the floating ***,population analysis was conducted,and it revealed a very low genetic diversity among samples from Subei Shoal and the Qingdao coast in the same year and a higher divergence among samples in different *** results showed the efficiency of 2b-RAD in the exploration of SNPs in *** and provided the first genome-wide scale evidence for the origin of the large-scale green tides on the Qingdao *** study improved our understanding of the reproductive strategy and genetic diversity of the green tide causative species and will help further reveal the biological causes of the green tide in China.

关键词： green tide Ulva prolifera 2b-RAD single-nucleotide polymorphism(SNP) reproductive strategy genetic diversity

NIPAM改性PEDOT:PSS导电聚合物的制备及在电致变色器件中的应用

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精细化工 2023年第8期40卷 1697-1703页

作者：汤朋韦铭孙靖江张旭阳钱海宁王向伟哈尔滨工程大学材料科学与化学工程学院黑龙江哈尔滨150001 青岛科技大学高分子科学与工程学院山东青岛266045 哈尔滨工程大学青岛创新发展中心山东青岛266400

以柔性疏水小分子N-异丙基丙烯酰胺(NIPAM)对聚苯乙烯磺酸盐(PSS)进行共聚改性,制备了一系列聚[(苯乙烯磺酸盐)-共-异丙基丙烯酰胺][P(SS-co-NIPAM)],并以其为模板采用氧化聚合法与3,4-乙烯二氧噻吩(EDOT)制备了导电聚合物PEDOT:P(SS-co... 详细信息

以柔性疏水小分子N-异丙基丙烯酰胺(NIPAM)对聚苯乙烯磺酸盐(PSS)进行共聚改性,制备了一系列聚[(苯乙烯磺酸盐)-共-异丙基丙烯酰胺][P(SS-co-NIPAM)],并以其为模板采用氧化聚合法与3,4-乙烯二氧噻吩(EDOT)制备了导电聚合物PEDOT:P(SS-co-NIPAM)。与PEDOT:PSS薄膜相比,NIPAM摩尔分数(以对苯乙烯磺酸钠物质的量为基准,下同)为15%时,PEDOT:P(SS-co-NIPAM)薄膜平均透光率保持在80%左右,水接触角从18.5°增至39.0°,疏水性提高,并且弯曲1000次后方阻变化量为5.71 kΩ/sq,远小于PEDOT:PSS薄膜(10.60 kΩ/sq)。以NIPAM摩尔分数为15%的PEDOT:P(SS-co-NIPAM)薄膜作为离子储存层的电致变色器件的光学对比度(ΔT)为9.83%,循环800次后ΔT仍达到9.55%,衰减量为0.28%,衰减量与PEDOT:PSS器件相当,说明NIPAM共聚改性能改善PEDOT:PSS导电聚合物的柔韧性和疏水性,以其作为离子储存层的器件可维持优异的电致变色性能。

关键词：聚3 4-乙烯二氧噻吩电致变色柔韧性疏水性导电聚合物功能材料

Spatial patterns of zooplankton abundance,biovolume,and size structure in response to environmental variables:a case study in the Yellow Sea and East China Sea

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Journal of Oceanology and Limnology 2024年第1期42卷 113-127页

作者： Song sun Haochen XIAN Xiaoxia sun Mingliang ZHU Mengtan LIU Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Jiaozhou Bay National Marine Ecosystem Research Station Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for Marine Ecology and Environmental Science Laoshan LaboratoryQingdao 266237China

The Yellow Sea(YS)and East China Sea(ECS)are highly dynamic marginal seas of the northwestern Pacific *** gain an in-depth understanding of zooplankton community structure,zooplankton abundance,biovolume,and size structure in summer 2017 in the YS and ECS were assessed using ZooScan imaging *** abundance and biovolume ranged 2.94–1187.14 inds./m^(3)and 3.13–3438.51 mm^(3)/m^(3),*** on the biovolume data of the categorized size classes of 26 identified taxonomic groups,the zooplankton community was classified into five groups,and each group was coupled with distinctive oceanographic *** the influence of the Yellow Sea Cold Water Mass,the Yellow Sea offshore group featured the lowest bottom temperature(10.84±3.42℃)and the most abundant Calanoids(mainly in the 2–3 mm size class).In the Yellow Sea inshore group,Hydrozoans showed the largest biovolume and dominated in the 3–4-mm and>5-mm size *** East China Sea offshore group,which was affected by the Kuroshio Branch Current,featured high temperature and salinity,and the lowest bottom dissolved oxygen(2.58±0.5 mg/L).The lowest values of zooplankton abundance and biovolume in the East China Sea offshore group might be attributed to the bottom dissolved oxygen *** East China Sea inshore group,which was mainly influenced by the Zhejiang-Fujian Coastal Current and Changjiang Diluted Water,was characterized by high chlorophyll a and the largest biovolume of carnivorous Siphonophores(280.82±303.37 mm^(3)/m^(3)).The Changjiang River estuary offshore group showed the most abundant Cyclopoids,which might be associated with the less turbid water mass in this *** temperature was considered the most important factor in shaping the size compositions of Calanoids in different groups.

关键词： zooplankton size structure ZooScan Yellow Sea East China Sea

Effect of nourishing Yin and purging fire Chinese herbal mixture on delaying light-induced premature puberty in rats

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Journal of Traditional Chinese Medicine 2024年第1期44卷 95-102页

作者： sun Yanyan XUE Yuanyuan sun Wen WANG Yonghong YU Jian Department of Traditional Chinese Medicine Children's Hospital of Fudan UniversityShanghai 201102China Department of Pediatrics Zibo Central HospitalZibo 255000China

OBJECTIVE:To elucidate the mechanism of the nourishing Yin and purging fire Chinese herbal mixture(NYPF)in delaying light-induced premature puberty in ***:Twenty-one days old female SpragueDawley rats were randomly assigned to normal group(N),long light exposure group(L),NYPF and normal saline group(NS).Rats in the L,NYPF and NS groups were exposed to 16 h:350 lux light/8 h:dark,while rats in the N group were exposed to 12 h:50 lux light/12 h:*** and normal saline was administered to the rats in the NYPF group or NS group,respectively,from day *** rats in every group were sacrificed at 9 *** day 28(P28),on the day when rat's vulva opened in the L group(L-VO),on the day when the first estrous interphase occurred in rats of L group(L-E1),and on the day when the second estrous interphase occurred in rats of L group(L-E2),***:On day 34,all rats in the L group,80%of rats in the NS group,40%of rats in the N group,and 20%of rats in the NYPF group showed complete opening of the *** P28,m RNA level of hypothalamic kisspeptin(Kiss-1)in the L group was significantly higher than that in the N group(P<0.05).The rats in the L and NS groups had significantly lower hypothalamic argininephenylalanine-amide(RFamide)-related peptide 3(RFRP-3)m RNA levels than those in the N group(P<0.05),whereas RFRP-3 m RNA level was significantly higher in the NYPF group than that in the L group(P<0.05).At L-VO,the ovarian index of the L and NS groups was significantly higher than that of the N group(P<0.05)and estradiol(E2)level of the NYPF group was significantly lower than that of the N and NS groups(P<0.05);hypothalamic Kiss-1 m RNA level in the L and NS groups was significantly higher than that in the N and NYPF groups(P<0.05),whereas hypothalamic RFRP-3 m RNA level in the L,NYPF,and NS groups was significantly lower than that in the N group(P<0.05).At L-E1,E2 level of the L and NS groups was significantly higher than that of the N group(P<0.01),wher

关键词： light puberty precocious kisspeptins RFRP-3 melatonin Nourishing Yin and Purging Fire

Natural polysaccharide-based smart CXCR4-targeted nano-system for magnified liver fibrosis therapy

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Chinese Chemical Letters 2024年第2期35卷 341-346页

作者： Liqiong sun Xinping Luo Chenxi Zhou Zhanwei Zhou Minjie sun College of Horticulture Nanjing Agricultural UniversityNanjing 210095China NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients State Key Laboratory of Natural MedicinesDepartment of PharmaceuticsChina Pharmaceutical UniversityNanjing 210009China

Activated hepatic stellate cells(aHSCs),the main source of extracellular matrix deposition,are key targets in liver ***,no effective drug specific to aHSCs has been clinically applied due to poor drug delivery ***,we designed a CXC chemokine receptor 4(CXCR4)-targeted reactive oxygen species(ROS)-responsive platform AMD-Dex-ROS-responsive-sorafenib(ARS)based on natural polysaccharide and thioctic acid frame,which can deliver anti-fibrosis drug represented by sorafenib specifically to aHSCs on account of CXCR4 over-expression on aHSCs,and smartly disassemble via ROS-responsive thioketal rupture relying on high intracellular ROS in HSCs,realized on-demand drug release and effective liver fibrosis ***,in this platform,the CXCR4 antagonist AMD3100 not only enhanced aHSCs targeting efficiency of sorafenib but also effectively magnified the aHSCs elimination of sorafenib by blocking stroma cell derived factor-1(SDF-1)/CXCR4-induced aHSCs protection,resulting in synergistic anti-fibrosis *** platform provided a new approach for drug delivery system design and liver fibrosis treatment.

关键词： Nanoparticle CXCR4 antagonism ROS-responsive Hepatic stellate cells Liver fibrosis

Online control for pressure regulation of oxygen mask based on neural network

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Control Theory and Technology 2024年第3期22卷 487-501页

作者： Ligan Zhao Qinglin sun Hao sun Jin Tao Zengqiang Chen College of Artificial Intelligence Nankai UniversityTianjin 300350China Silo Al Helsinki 00100Finland

The aviation oxygen mask,which has a small volume of less than 1L and strong air tightness,imposes extremely high requirements on control performance of the oxygen *** on analyses of the operation principle of oxygen supply system,the dynamic model is established through the combination of mechanism analysis and experimental *** that the traditional fixed-parameter controllers are difficult to meet the control requirements with changes in pulmonary ventilation,this paper presents an online feedback controller based on neural network compensation(NNC),with connection weights that can be updated without *** mathematical simulations at different respiratory parameters,such as respiratory rate,are performed to verify the superior lower inspiratory resistance of controller with *** terms of hardware,an embedded AI control platform is to complete the experimental ***,the work may have downward compatibility to achieve stable oxygen supply in civil fields,such as medical ventilators,high-altitude expeditions.

关键词： Neural network Online control Pressure regulation AI platform

Rate-limiting factors in hydrate decomposition through depressurization across various scales:A mini-review

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Chinese Journal of Chemical Engineering 2024年第3期67卷 206-219页

作者： Xian sun Peng Xiao Qinfeng Shi Lingban Wang Zhenbin Xu Yuhao Bu Xiaohui Wang Yifei sun Changyu sun Guangjin Chen State Key Laboratory of Heavy Oil Processing China University of Petroleum(Beijing)Beijing 102249China Jining University Jining 273115China

Natural gas hydrate is an energy resource for methane that has a carbon quantity twice more than all traditional fossil fuels ***,their practical application in the field has been limited due to the challenges of long-term preparation,high costs and associated *** studies,on the other hand,offer a safe and cost-effective means of exploring the mechanisms of hydrate dissociation and optimizing exploitation *** hydrate decomposition is a complicated process along with intrinsic kinetics,mass transfer and heat transfer,which are the influencing factors for hydrate decomposition *** identification of the rate-limiting factor for hydrate dissociation during depressurization varies with the scale of the reservoir,making it challenging to extrapolate findings from laboratory experiments to the actual *** review aims to summarize current knowledge of investigations on hydrate decomposition on the subject of the research scale(core scale,middle scale,large scale and field tests)and to analyze determining factors for decomposition rate,considering the various research scales and their associated influencing factors.

关键词： Gas hydrate Rate-limiting factors Research scale Depressurization Decomposition

In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions

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Journal of Bioresources and Bioproducts 2024年第1期9卷 90-101页

作者： Bianjing sun Ping Wang Jingang Zhang Jianbin Lin Lingling sun Xiaokun Wang Chuntao Chen Dongping sun Institute of Chemicobiology and Functional Materials School of Chemistry and Chemical EngineeringNanjing University of Science and TechnologyNanjing 210094China Zhonghao Chenguang Chemical Research Institute Co. Ltd.Zigong 643201China

Bacterial cellulose(BC)hydrogel spheroid plays a significant role in diverse fields due to its spatial 3D structure and *** the present work,a series of BC spheroids with controllable size and shape was obtained via an in situ *** factors for fabricating BC spheroid in-cluding inoculum concentration of 1.35×10^(3)CFU/mL,shaking speeds at 100 r/min,and 48-96 h incubation time during the biosynthetic process,were comprehensively *** operable mechanism model for tuning the size of BC spheroids from 0.4 to 5.0 mm was proposed with a fresh feeding medium strategy of dynamic *** resulting BC spheroids exhibit an inter-active 3D network of nanofibers,a crystallinity index of 72.3%,a specific surface area of 91.2 m^(2)/g,and good *** study reinforces the understanding of BC spheroid forma-tion and explores new horizons for the design of BC spheroids-derived functional matrix materials for medical care.

关键词： In situ biosynthesis Bacterial cellulose Hydrogel Spheroid Tunable dimension

Cooperative game penetration guidance for multiple hypersonic vehicles under safety critical framework

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Chinese Journal of Aeronautics 2024年第1期37卷 247-255页

作者： Jieqing CHEN Ruisheng sun Yu LU School of Energy and Power Engineering Nanjing University of Science and TechnologyNanjing 210094China

The rapid development of the anti-missile weapon technology brings new challenges to the cooperative penetration strategy solution and the guidance law design for Hypersonic Vehicles(HVs).This paper studies the coordinated game penetration guidance problem for multiple hypersonic vehicles faced with space threat areas.A scheme for seeking cooperative game penetration guidance strategy under safety critical control framework is *** this scheme,a multiHV cooperative game model is proposed in a minimum optimization form which can simplify the solving process and accelerate the computing ***,a second-order control barrier function is developed to transform the implicit nonlinear constraints of the proposed model into linear *** order to obtain better performance of guidance strategy,a composite guidance law under the safety critical control framework is presented to allocate guidance strategies appropriately in the whole *** is shown that the proposed scheme can guarantee successful penetration while avoiding threat ***,a comparative simulation with a two-on-three game is conducted to verify the effectiveness of the proposed method.

关键词： Hypersonic vehicles Safety critical control Penetration game Space constraints Cooperative guidance