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Genetic diversity analysis of Lepidium sativum(Chandrasur) using inter simple sequence repeat(ISSR) markers

Journal of Forestry Research 2015年第1期26卷 107-114页

作者： Amandeep Kaur Rakesh Kumar suman rani Anita Grewal Department of Biotechnology University Institute of Engineering & Technology Kurukshetra University

Lepidium sativum（commonly known as garden cress） belongs to the family Brassicaceae. It is a fastgrowing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic,hypoglycemic, nephrocurative and nephroprotective activities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat（ISSR） markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and reproducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were polymorphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were *** analysis based on unweighted pair-group method,arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of *** and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range32–72 % genetic relatedness among the 18 genotypes.

关键词： ISSR Genetic diversity Polymorphism Lepidium sativum Cluster analysis

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Ultrasonic study on molecular interactions in binary mixtures of formamide with 1-propanol or 2-propanol

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Chinese Journal of Chemical Engineering 2015年第4期23卷 689-698页

作者： Manju rani suman Gahlyan Ankur Gaur Sanjeev Maken Department of Chemical Engineering Deenbandhu Chhotu Ram University of Science and Technology Department of Chemistry Deenbandhu Chhotu Ram University of Science and Technology Department of Chemical Engineering Motilal Nehru National Institute of Technology

Ultrasonic speeds have been measured at 298.15 K and 308.15 K for mixtures of formamide ＋ 1 -propanol or 2-propanol. For an equimolar mixture, excess molar compressibility follows the sequence of l-propanol 〉 2-propanol. The ultrasonic speed data are correlated by various correlations such as Nomoto＇s relation, van Dael＇s mixing relation and impedance dependence relation, and analyzed in terms of Jacobson＇s free length theory and Schaaffs collision factor theory. Excess isentropic compressibility is calculated from ex- perimental ultrasonic speed data and previously reported excess volume data. The excess molar ultrasonic speed and isentropic compressibility values are fitted to Redlich-Kister polynomial equation. Other proper- ties such as molecular association, available volume, free volume, and intermolecular free length are also calculated. The excess isentropic compressibility data are also interpreted in terms of graph theoretical ap- proach. The calculated isentropic compressibility values are well consistent with the experimental data. It is found that the interaction between formamide and propanol increases when hydroxyl group attached to a carbon atom has more -CH3 groups.

关键词： Ultrasonic speedGraph theoretical approachFormamidePropanollntermolecular interaction

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