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Hypothemycin production and its derivatives diversifying of <i>Aigialus parvus</i>BCC 5311 influenced by cultural condition

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Advances in Bioscience and Biotechnology 2013年第12期4卷 1049-1056页

作者： Kanokarn Kocharin sumalee supothina Wai Prathumpa National Center for Genetic Engineering and Biotechnology Na-tional Science and Technology Development Agency Pathum Thani Thailand

Many metabolites produced by various microorganisms have proven their usefulness in the area concerning human health. However, most of their diverse natural compound biosyntheses are hardly discovered. These metabolites might have specific or novel functions and these diverse active compounds can be achieved by biosynthesis, semi-biosynthesis, or chemical synthesis. A strategy to exploit the biosynthesis potential of a fungal strain is to use various culture conditions and to evaluate the chemical profiles of the culture extracts. The value of this approach was demonstrated with the fungal strain Aigialus parvus BCC 5311, producer of hypothemycin, aigialospirol, and aigialomycin A-D. The optimization of hypothemycin production and its derivative diversity by Aigialus parvus BCC 5311 was carried out using qualitative (general factorial design) and quantitative analysis (two-level fractional factorial design). Qualitative analysis revealed that soluble starch and yeast extract were shown to be the best carbon and nitrogen source respectively for the production of hypothemycin, aigialospirol and aigialomycin A-D. Quantitative analysis showed that the initial pH of culture medium is the most important factor that affects the production of hypothemycin and its derivatives (aigialospirol and aigialomycin A-D) production. Optimal medium composition used in a 5 L bioreactor generated a specific growth rate of A. parvus BCC 5311 of 0.0295 h-1, biomass yield of 1.6 g×gstarch-1, hypothemycin yield of 13.6 mg×gbiomass-1, and hypothemycin production rate of 0.6 mg×L-1×day-1. The maximum concentration of 58.0 mg×L-1 of hypothemycin was obtained at 120 h of culturing. Furthermore, the Aigialomycin A-D and Aigialospirol obtained were diversified towards various cultural conditions used. The high amount of hypothemycin produced and the diversity of derivatives obtained from this study should be useful for future mass production.

关键词： Hypothemycin Aigialus parvus Aigialomycin Aigialospirol Fractional Factorial Design Derivative Diversifying

Beauvericin production by the Lepidoptera pathogenic fungus Isaria tenuipes: Analysis of natural specimens, synnemata from cultivation, and mycelia from liquid-media fermentation

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Natural Products and Bioprospecting 2011年第3期1卷 112-115页

作者： sumalee supothina Urarat SRISANOH Sutichai NITHITHANASILP Kanoksri TASANATHAI J.Jennifer LUANGSA-ARD Chun-Ru LI Masahiko ISAKA National Center for Genetic Engineering and Biotechnology(BIOTEC) 113 Thailand Science ParkPhaholyothin RoadKlong LuangPathumthani 12120Thailand Anhui Provincial Key Laboratory for Microbial Control Anhui Agricultural UniversityHefei 230036AnhuiChina

Beauvericin was analyzed in three forms of the Lepidoptra pathogenic fungus Isaria tenuipes(4 isolates):(a)natural specimen,(b)cultivated synnemata on rice media,and(c)mycelia from fermentation in liquid *** was detected in very low amounts in all tested natural *** on rice contained much higher concentrations of beauvericin than the corresponding natural materials,although the concentrations were lower than mycelia from liquid *** results casted a caution that beauvericin concentration should be carefully checked,as a possible toxic constituent,upon mass production of a selected strain of Isaria tenuipes for health food purposes.

关键词： beauvericin Isaria tenuipes chemotaxonomy toxin

心理契约、组织公民行为与任务绩效的关系研究

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心理契约、组织公民行为与任务绩效的关系研究

作者： Chanwattanakit sumalee（柳素莉）华南理工大学

学位级别：硕士

经济全球化推动着跨国企业的发展，同时，随着组织管理的不断变革，心理契约、组织公民行为以及任务绩效等等都成为了各国学者研究的热点。泰国与中国一直都有密切的贸易往来，泰国在华公司的数量也在不断增长。由于中泰企业的员工之间... 详细信息

经济全球化推动着跨国企业的发展，同时，随着组织管理的不断变革，心理契约、组织公民行为以及任务绩效等等都成为了各国学者研究的热点。泰国与中国一直都有密切的贸易往来，泰国在华公司的数量也在不断增长。由于中泰企业的员工之间的差异，企业内部的员工管理也存在差别，这些差异通过员工心理与行为显著地影响着员工的任务绩效。心理契约指的是员工与组织间关系的一种未书面化的契约，一种期望。在亚洲文化的企业中，心理契约很大程度上会影响员工的态度和行为，进而影响任务绩效。本文从组织公民行为的角度出发，尝试探索心理契约与员工任务绩效之间的相关关系。在阅读和梳理现有的相关文献的基础上，选用组织公民行为作为两者之间的中介变量，建立了心理契约、组织公民行为、任务绩效三者之间的关系模型以及相应的理论假设。使用调查问卷的方法，收集泰国在华企业员工的数据，并运用统计软件SPSS17.0对调查取得的数据进行统计与分析。通过研究，本文得出了以下主要结论：（1）年龄与性别差异对心理契约、组织公民行为以及任务绩效的影响不显著，而学历差异对这三者的影响均显著;（2）心理契约与组织公民行为之间存在显著的正相关关系;（3）组织公民行为与任务绩效之间存在显著的正相关关系;（4）心理契约与任务绩效存在显著的正相关关系，组织公民行为在心理契约与任务绩效之间起部分中介作用。

关键词：心理契约组织公民行为任务绩效

Development of the Lower Central Plain of Thailand with History of Human Settlements： Evidence from Pollen, Spores and Diatoms

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Journal of Geological Resource and Engineering 2015年第2期3卷 98-107页

作者： Wickanet Songtham sumalee Musika Dallas C. Mildenhall Ursula A. Cochran Darya Kojevnikova Northeastern Research Institute of Petrified Wood and Mineral Resources Nakhon Ratchasima Rajabhat University NakhonRatchasima 30000 Thailand GNS Science Lower Hutt PO Box 30 368 New Zealand Institute of Geology Azerbaijan National Academy of Sciences Baku AZ1 143 Azerbaijan

论电子媒体对泰国学生学习汉语的作用——基于电子媒体与传统教学方法之比较

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经济与社会发展 2011年第6期9卷 142-145页

作者： sumalee pongtiyapaiboon 黄旺贵泰国碧武里皇家大学教育学院泰国华欣中学

目前,汉语已经成为泰国一门非常重要的语言,全国各地的中小学校都纷纷开设了汉语课程,泰国的很多学生都纷纷学习汉语。但随之而来广大汉语教师所面临的问题是泰国学生不能很好地记住汉字,不能正确地书写汉字,这成为了他们学习汉语的障... 详细信息

目前,汉语已经成为泰国一门非常重要的语言,全国各地的中小学校都纷纷开设了汉语课程,泰国的很多学生都纷纷学习汉语。但随之而来广大汉语教师所面临的问题是泰国学生不能很好地记住汉字,不能正确地书写汉字,这成为了他们学习汉语的障碍。传统的教学方法并不能改变现状,唯有使用电子媒体教学才能很好地帮助泰国学生提升书写汉字的能力和耐久性。

关键词：电子媒体书写汉字的能力耐久性