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Characterization of a Novel RING-Type Ubiquitin E3 Ligase GhRING2 Differentially Expressed in Cotton Fiber

作者： (印)苏库马尔·莫拉里塔兰(sukumar Muralidharan)

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American Journal of Plant Sciences 2014年第21期5卷 3364-3379页

作者： Shiva Soma Chuan-Yu Hsu sukumar Saha Johnie N. Jenkins Din-Pow Ma Department of Biochemistry Molecular Biology Entomology and Plant Pa-thology Mississippi State University Mississippi State USA Institute for Genomics Biocomputing & Biotechnology Mississippi State Uni-versity Mississippi State USA Crop Science Research Laboratory Agricultural Research Service United States Department of Agriculture Mississippi State USA Department of Plant and Soil Sciences Mississippi State University Mississippi State USA

The ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By employing affymetrix microarray analysis, we have identified a novel ubiquitin ligase E3 gene GhRING2 that is differentially expressed between two Gossypium hirsutum lines-Texas Marker-1 (TM-1) and Chromosome Substitution Line CS-B25. The CS-B25 line has chromosome 25 from G. barbadense substituted into TM-1. The complete nucleotide sequences of GhRING2 along with its 5’-flanking region were obtained by genomic walking. GhRING2 was highly expressed in elongating fiber, and GUS expression directed by the GhRING2 promoter was found in hypocotyls and young stems of transgenic Arabidopsis plants. Using a yeast two-hybrid assay GhRING2 was found to interact with a PROTODERMAL FACTOR1 (GhPDF1) protein. GhPDF1 was expressed preferentially in immature ovules and fiber initials, and the GhPDF1 gene had been suggested to play a role in cell fate determination and fiber development. Pull down and plasmid swap assays further confirmed the interaction between GhRING2 and GhPDF1. The expression and protein interaction data indicate that GhRING2 may be involved in the turnover of GhPDF1 and participation in the transition from initiation to elongation stages during fiber development. Our data strongly suggest that the ubiquitin-proteasome pathway may regulate cotton fiber growth and development. The nucleotide sequence data of GhRING2 in this article have been submitted to the Gen Bank Nucleotide Sequence Data Bases under the accession number BankIt 1,742,008 SeqKM 108,000.

关键词： Cotton Fiber Gossypium hirsutum G. barbadense RING-Type Ubiquitin Ligase Ubiquitination

中国柱花草炭疽病原菌遗传多态性的RAPD分析

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微生物学报 2003年第3期43卷 379-387页

作者：易克贤黄俊生刘国道 Pualine Weeds sukumar Charkraborty 中国热带农业科学院热带牧草研究中心儋州571737 中国热带农业科学院热带作物生物技术国家重点实验室海口571101 CSIRO Tropical Agriculture

在对中国柱花草炭疽病进行广泛调查和病原采样收集的基础上 ,利用RAPD分子标记技术对 43个代表性菌株进行了基因组DNA分析 ,并与 2 76份国外菌株进行了综合聚类分析。结果表明所用 8个引物的扩增片段位于 0 3～ 2 8kb之间 ,菌株间呈... 详细信息

在对中国柱花草炭疽病进行广泛调查和病原采样收集的基础上 ,利用RAPD分子标记技术对 43个代表性菌株进行了基因组DNA分析 ,并与 2 76份国外菌株进行了综合聚类分析。结果表明所用 8个引物的扩增片段位于 0 3～ 2 8kb之间 ,菌株间呈现显著的DNA多态性。以柱花草起源中心———南美的柱花草炭疽菌分类为基础 ,中国柱花草炭疽菌可划分成 3大类型即Ⅱ、Ⅲ、Ⅵ类。中国菌株与来自柱花草起源中心———南美的菌株相比之下 ,其生物多样性和遗传变异性则相对简单。就中国菌株而言海南菌株与广西、广东菌株相比多样性较丰富 ,中国柱花草胶孢炭疽菌正在出现种内遗传分化。从聚类结果看 ,通常来自于同一个地理区域或同一个寄主基因型的菌株聚成一类 ,即同一RAPD聚类组内的菌株通常来自于同一寄主基因型或同一地理区域。说明来自不同寄主基因型或物种的炭疽菌在遗传基因上具有专化性 ,而地理上隔离的国家或地区的柱花草炭疽病原菌各自具有相对独立的进化途径。

关键词：中国柱花草炭疽病原菌遗传多态性 RAPD分析豆科牧草

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为电器马达提供动力

今日电子 2007年第6期 49-51页

作者： V.sukumar 飞兆半导体公司

近一个世纪以来，感应马达一直是电器行业的动力提供部件。在新型的变频驱动器中，也有许多使用感应马达。但有部分新的电器开始采用更高效、更紧凑轻巧的马达系统。这些新型电器马达实际上可以划分为无刷直流（Brushless DC）马达和开... 详细信息

近一个世纪以来，感应马达一直是电器行业的动力提供部件。在新型的变频驱动器中，也有许多使用感应马达。但有部分新的电器开始采用更高效、更紧凑轻巧的马达系统。这些新型电器马达实际上可以划分为无刷直流（Brushless DC）马达和开关磁阻（switched reluctance）马达两大系列。

关键词：电器行业马达开关磁阻驱动器感应部件变频系统

Isolation, Identification and Characterization of Genes Differentially Expressed between Pima and Upland Cotton Fibers

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棉花学报 2002年第S1期14卷 47-47页

作者： Khairy M.SOLIMAN Allan ZIPF sukumar SAHA Department of Plant and Soil Science Alabama A&M UniversityNormalALDepartment of Plant and Soil ScienceAlabama A&M UniversityNormalALUSDA/ARS/CSRLMississippi StateUSA

The demand for high strength fiber in rawcotton has increased because of the widespreaduse of high speed spinning technology in theyarn and textile *** ofcotton fiber quality through

关键词： Cotton cotton spinning probes genetics yielding developmental differentially homologous remaining

更好的功率MOSFET带来更环保的功率产品

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电子与电脑 2005年第8期5卷 71-73页

作者： V. sukumar 飞兆半导体公司

由于市场预期石油价格将持续处于高位,全球再度掀起了提高电子产品效率的热潮.国际间许多能源和环境保护组织都制定有关技术规范,以提高电源、电机驱动和照明整流的效率.例如,中国的节能产品认证中心(CECP)、美国环保署(EPA)及澳大利亚... 详细信息

由于市场预期石油价格将持续处于高位,全球再度掀起了提高电子产品效率的热潮.国际间许多能源和环境保护组织都制定有关技术规范,以提高电源、电机驱动和照明整流的效率.例如,中国的节能产品认证中心(CECP)、美国环保署(EPA)及澳大利亚和其它国家的类似机构正进行相关协作.

关键词：功率MOSFET 美国环保署电子产品环境保护组织石油价格技术规范电机驱动认证中心节能产品

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为电器马达提供动力

中国电子商情 2007年第12期 44-47页

作者： V.sukumar 飞兆半导体公司

目前，家用电器中的马达驱动器日益复杂完善。为了应对效率更高、可靠性更强及成本更低的挑战，本文探讨了马达驱动和智能功率模块中组成部分的最新发展趋势。

关键词：家用电器马达智能功率模块发展趋势驱动器可靠性

Study on Threshold Patterns with Varying Illumination Using 1.3m Imaging System

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Intelligent Information Management 2010年第1期2卷 21-25页

作者： Vinesh sukumar Herbert L. HESS Ken V. NOREN Greg DONOHOE Suat AY 不详

Human eye can generally distinguish objects from each other or from their background, if the difference in luminance or color is large. This paper concentrates on the luminance portion and makes an attempt to characterize perception detection to varying contrast which is explained in contrast sensitivity terms. This is accomplished using sinusoidal test patterns. Influence of illumination on perception threshold is also shown in this paper. Practical measurements are done using a calibrated monitor with image capture accomplished with a 1/4' 1.3M camera module system.

关键词： perception threshold contrast sensitivity

Chromosome Substitution Lines:Useful Genomic Resources in Cotton

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棉花学报 2008年第S1期20卷 111-页

作者： SAHA sukumar JENKINS Johnie N MCARTY Jack C STELLY David USDA-ARS Crop Science Research Laboratory P.O.Box 5367 Mississippi State Dept.Soil & Crop Sciences TAMU College Station

The genus Gossypium is a very diverse one with about 50 ***,a very narrow germplasm base is presently being used for genetic improvement of Upland *** wild

关键词： CS

Bilateral tubal pregnancy: A diagnostic dilemma

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Open Journal of Obstetrics and Gynecology 2013年第9期3卷 639-641页

作者： Rahul Deb Mandal Sandip Ghosh sukumar Mitra Avijit Basak Prasun Naskar Kousik Seth Atin Halder Department of General Surgery Burdwan Medical College Burdwan India Department of Gynecology and Obstetrics Burdwan Medical College Burdwan India

Bilateral tubal ectopic pregnancy is very rare and usually occurs following ovulation stimulation. Moreover preoperative diagnosis is very difficult. We are presenting a case of bilateral tubal ectopic pregnancy occurring spontaneously. Hence careful follow-up with combination of color Doppler and serum beta HCG estimation of patients after laparoscopic or open surgery for ectopic pregnancies is needed to avoid such unusual event.

关键词： Bilateral Tubal Ectopic Diagnostic Dilemma Colourdoppler