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Isolation, purification and characterization of an N-acetyl-D-lactosamine binding mitogenic and anti-proliferative lectin from tubers of a cobra lily Arisaema utile Schott

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Advances in Bioscience and Biotechnology 2010年第2期1卷 79-90页

作者： Vikram Dhuna Kshitija Dhuna Jatinder singh Ajit Kumar Saxena Satyam Kumar Agrawal sukhdev singh kamboj 不详

Lectins are the carbohydrate-binding proteins of non-immune origin which have been the subject of intense investigation over the last few decades owing to the variety of interesting biological properties. Most of the lectins which have been purified and characterized from plants have been obtained from dicotyledons. In the present study a lectin was purified from tubers of a monocot plant Arisaema utile (AUL) Schott by affinity chromatography on asialofetuin-linked amino activated silica beads. AUL gave a single band in SDS-PAGE at pH 8.3 corresponding to subunit Mr 13.5 kDa. The native molecular mass of AUL was 54 kDa suggesting a homotetrameric structure. AUL gave multiple bands in isoelectric focusing and in native PAGE at pH 8.3. AUL was inhibited by N-acetyl-D-lactosamine (Lac NAc), a disaccharide and asialofetuin, a complex desialylated serum glycoprotein. When treated with denaturing agents, the lectin was stable in the presence of urea (3 M), thiourea (4 M) and guanidine HCl (4 M). AUL was a glycoprotein with a carbohydrate content of 1.2%. Complete loss of activity was observed upon modification of tryptophan residues of the lectin. The activity was reduced to 25% after modification of tyrosine. Chemical modification of arginine, histidine, serine and cysteine residues of AUL did not affect its activity. Using Far UV CD spectra the estimated secondary structure was 37% α-helix, 25% β-sheet and 38% random contributions. The lectin showed potent mitogenic response towards human lymphocytes. In vitro anti-proliferative assay using 11 human cancer cell lines resulted in 50% inhibition of six cell lines viz. SW-620, HCT-15, SK-N-SH, IMR-32, Colo-205 and HT-29 at 38, 42, 43, 49, 50 and 89 µg/ml, respectively.

关键词： N-acetyl-D-lactosamine Arisaema Utile Affinity Purification Anti-Proliferative Asialofetuin Chemical Modification Lectin Mitogenicity

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Isolation, purification and characterization of an N-acetyl-D-lactosamine binding mitogenic and anti-proliferative lectin from tubers of a cobra lily Arisaema utile Schott

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Advances in Bioscience and Biotechnology（生命科学与技术进展） 2010年第2期

作者： Vikram Dhuna Kshitija Dhuna Jatinder singh Ajit Kumar Saxena Satyam Kumar Agrawal sukhdev singh kamboj

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A fuzzy time series forecasting method induced by intuitionistic fuzzy sets

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International Journal of Modeling, Simulation, and Scientific Computing 2015年第4期6卷 130-152页

作者： Sanjay Kumar sukhdev singh Gangwar G.B.Pant University and Agriculture and Technology Pantnagar UttarakhandIndia

Intuitionistic fuzzy sets(IFSs)are well established as a tool to handle the hesitation in the decision *** this research paper,fuzzy sets induced by IFS are used to develop a fuzzy time series forecasting model to incorporate degree of hesitation(nondeterminacy).To improve the forecasting accuracy,induced fuzzy sets are used to establish fuzzy logical *** verify the performance of the proposed model,it is implemented on one of the benchmarking time series ***,developed forecasting method is also tested and validated by applying it on a financial time series *** order to show the accuracy in forecasting,the method is compared with other forecasting methods using different error measures.

关键词： Fuzzy time series intuitionistic fuzzy sets nondeterminacy induced fuzzy set data models.

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Genetic Variation for Anaerobic Germination and Emergence from Deeper Soil Depth in Oryza nivara Accessions

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Rice science 2022年第4期29卷 304-308页

作者： Revanayya MGOTHE Dharminder BHATIA Akashdeep kamboj Nitika SANDHU Buta singh DHILLON Department of Plant Breeding and Genetics Punjab Agricultural UniversityLudhiana 141004India School of Agricultural Biotechnology Punjab Agricultural UniversityLudhiana 141004India

Anaerobic germination and emergence from deeper soil depth are two important traits for breeding rice suitable for direct seeded *** order to evaluate genetic variation for these traits,a total of 300 Oryza nivara accessions were evaluated along with checks,of which,159 accessions germinated under anaerobic conditions indicating presence of immense variability in *** 159 accessions,69 germinated in submerged conditions and 90 germinated after removing water with availability of aerobic conditions,indicating two different mechanisms operating in ***,out of 300 *** accessions,218 germinated from 6 cm and 95 germinated from 8 cm soil *** accessions that germinated from deeper soil depth had longer mesocotyl and coleoptile lengths.

关键词： soil breeding removing

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Ipsilateral dislocation of the radial head associated with fracture of distal end of the radius： a case report and review of the literature

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Chinese Journal of Traumatology 2013年第3期16卷 182-185页

作者： Vinay Gupta Zile singh Kundu Maneet Kaur Pradeep kamboj Jitesh Gawande Department of Orthopaedics Fortis Healthcare MohaliPunjab 160062 Inida Department of Orthopaedics Physical MedicineParaplegia and Rehabilitation Pt. B.D.Sharma Post Gradu-ate Institute of Medical Sciences Rohtak 124001 India Department of Emergency Medicine Dayanand Medi-cal College and Hospital Ludhiana 141001 Inida )

Dislocation of the radial head in adults is uncommon. A simultaneous dislocation of the radial head and fracture of the ipsilateral distal end of radius with no other associated injuries is extremely rare. As far as we know, such an injury after an unusual mode of injury has been seldom reported in the English literature. We report such a case without any associated injuries or comorbidity. Closed reduction was performed within two hours after injury and results were satisfactory. Immobilisation was continued for3 weeks. Gradual mobilisation was started after removal of the plaster under the supervision of a physiotherapist. At 6 months＇ follow-up, the patient had no residual pain at the elbow with full flexion ＆ extension. Almost full supination with a restriction of last 10 degrees of pronation was achieved. There was no evidence of instability of the elbow.

关键词： Radial heads Dislocations Radius fractures

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Fracture Bilateral Talus in Children

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Surgical Science 2013年第9期4卷 405-409页

作者： Vinit Verma Amit Batra Pradeep kamboj Suresh Bhuriya Raj singh Sumit Kumar Rakesh Gupta Narender Kumar Magu Pt. B. D. Sharma PGIMS Rohtak Rohtak India

Talar body injuries are rare, particularly in children. To our knowledge, there has not been a single case report of bilateral talus fracture in a child till date. We report two cases of fracture bilateral talus in children. The first case is of a fracture separation of the distal tibial epiphysis and a fracture of the body of the talus with subluxation of ankle on right side and a fracture neck of talus on left side. The second is fracture bilateral talus with epiphyseal injury of left distal tibia. A minimal or undisplaced fracture of talus is less likely to undergo avascular necrosis than a displaced fracture but even with optimal treatment, avascular necrosis may still occur. It is of prime significance that these fractures should be diagnosed well in time to avert complications. Therefore an appropriate length of follow-up is required.

关键词： Paediatric Talus Fracture Bilateral Avascular Necrosis

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Mechanized Transplanting of Rice(Oryza sativa L.)in Nonpuddled and No-Till Conditions in the Rice-Wheat Cropping System in Haryana,India

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American Journal of Plant Sciences 2013年第12期4卷 2409-2413页

作者： Baldev Raj kamboj Dharam Bir Yadav Ashok Yadav Narender Kumar Goel Gurjeet Gill Ram K.Malik Bhagirath singh Chauhan Krishi Vigyan Kendra CCS Haryana Agricultural UniversityYamuna NagarIndia Regional Research Station CCS Haryana Agricultural UniversityKarnalIndia Department of Agronomy CCS Haryana Agricultural UniversityHisarIndia School of Agriculture Food&WineUniversity of AdelaideAdelaideAustralia CIMMYT-IRRI CSISA Bihar Hub PatnaIndia Crop and Environmental Sciences Division International Rice Research InstituteLos BanosPhilippines

The common practice of establishing rice in the rice-wheat system in India is manual transplanting of seedlings in the puddled soil. Besides being costly, cumbersome, and time consuming, puddling results in degradation of soil and the formation of a hard pan, which impedes root growth of subsequent upland crops. In addition, decreased availability and increasing cost of labor have increased the cost of rice cultivation through conventional methods. Because of these concerns, there is a need for mechanized transplanting of rice which is less labor-intensive and can ensure optimum plant population under nonpuddled and/or no-till conditions. A large number of on-farm trials were conducted at farmers’ fields in Haryana, India, from 2006 to 2010 to evaluate the performance of the mechanical transplanted rice (MTR) under nonpuddled and no-till situations as compared to conventional puddled transplant rice (CPTR). Compared with CPTR, nonpuddled MTR produced 3%-11% higher grain yield in different years. Rice cultivars, viz. HKR47, HKR127, PR113, PR114, PB1, PB1121, CSR30, and Arize6129, performed consistently better under nonpuddled MTR as compared to CPTR. Performance of different cultivars (PR113, PR114, HKR47, and Pusa 44) was also better under no-till MTR as compared to CPTR. The “basmati” cultivar CSR30 performed equally in no-till MTR and CPTR systems. The results of our study suggest that rice can be easily grown under nonpuddled and no-till conditions with yield advantages over the CPTR system. Even in the case of similar yield between CPTR and MTR systems, the MTR system will help in reducing labor requirement and ultimately, will increase overall profits to farmers.

关键词： Farmer Participatory Research Self Propelled Paddy Transplanter Double No-Till Rice-Wheat System Nonpuddled Transplanted Rice

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印度Ispat CSP工厂生产优质热轧卷

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钢铁 2004年第Z1期39卷 393-400页

作者： Fritz-Peter Pleschiutschnigg Vilas Vishnu Jamnis sukhdev Raj Talwar Atul Kumar Misra Ratna Prasad V. Atluri R. B. singh Prem Shankar Rajendra K. Verma Rajesh K. Goyal Vidiya P. Mishra Bidyapati Deepu B. Peter Meierling Joseph Pleschiutschnig United Steel Center Operations SIP&HSM Operations Steel Melt Shop Operations Caster Operations Process Quality Rolling Mill Operations 　　　　 Ispat Industries Ltd Ispat Industries Ltd

　　1998年,印度Ispat公司开始投产CSP生产线(图1).这个生产线原计划年产300万t热轧卷,年产210万t高炉提供铁水.Midren装置年产160万吨海绵铁.……

关键词：印度工厂生产热轧卷生产线海绵铁装置投产铁水高炉

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