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A novel ascosporogenous yeast species,Zygosaccharomyces siamensis,and the sugar tolerant yeasts associated with raw honey collected in Thailand

引用

Fungal Diversity 2012年第1期52卷 123-139页

作者： sujinan saksinchai Motofumi Suzuki Panuwan Chantawannakul Moriya O.hkuma Saisamorn Lumyong The Graduate School Chiang Mai UniversityChiang Mai 50200Thailand Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Microbe Division/Japan Collection of Microorganisms(JCM) RIKEN BioResource CenterWakoSaitama 351-0198Japan

Diversity of yeasts in association with bees and their food sources has been explored during the last *** Thailand,there has been no study of yeast identification in honey and ***,a total of 186 yeast strains were isolated from 37 honey samples of 12 different bee *** the basis of morphological and physiological characteristics,55 representative strains were chosen and identified by sequence analysis of the 26S rDNA D1/D2 domain and the ITS *** data were compared with the published sequences and the results showed the occurrence of 19 ascomycetous and 1 basidiomycetous yeast *** strains of the new species were *** analysis of the 26S rDNA D1/D2 sequence revealed that they were conspecific and most closely related to Zygosaccharomyces *** on the ITS sequence,the new species was clustered with the type"and clearly distinguished from the type!.Sequence analysis of combined ITS-26S rDNA D1/D2 showed similar *** occurrence of these two types,with a divergence of more than 1%in their sequences,and low DNA relatedness among them suggested that members of the typeβcan be regarded as separate *** analysis of the morphological and physiological characteristics was *** formation was observed on acetate agar and Gorodkowa *** new Zygosaccharomyces species differed physiologically from *** in 4 assimilation *** data supports the hypothesis that the new species,Zygosaccharomyces siamensis,is a novel ascosporogenous *** type strain is JCM 16825T(=CBS 12273T)and a description is given here.

关键词： Ascosporogenous yeast Zygosaccharomyces siamensis Sugar tolerant yeasts Raw honey Thailand

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