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Cloning and characterization of a new actin gene from Oryza sativa L.

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Progress in Natural Science:Materials International 2004年第10期14卷 27-34页

作者： LIANG Weihong,TANG Chaorong and wu naihu (institute of {3. and {4. biology,chinese academy of Sciences,Beijing100080,china) institute of genetics and developmental {3. Chinese Academy of Sciences Beijing 100080 China

Using Rho family member osRACD as bait,a new member of actin gene family-Act was isolated from Oryza sativa by yeast two-hybrid *** full-length cDNA was cloned with5′RACE technology,which contains an open reading frame of113.bp with a predicted protein of3.7amino *** alignment revealed96%to81.8%identities with some known actin proteins in *** method of bioinformatics was used to analyze the protein modification sites,structure and evolution of the *** blot analysis showed that Act is a single-copy gene in the *** result of RT-PCR showed it is ubiquitously expressed in root,shoot,callus and panicle in a temporal *** relationship between Rho family and actin family in evolution and function was also studied.

关键词： rice panicle yeast two-hybrid actin Rho gene clone

A new trick to tune down TGFβ signal

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Cell Research 2004年第5期14卷 439-440页

作者： Jian zhang* Center for developmental biology, {3. of {4. and developmental biology, chinese academy of Sciences, No. 3.Nan-Yi-Tiao, Zhong guan Cun, Beijing 100080, china Center for developmental biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences No. 3 Nan-Yi-Tiao Zhong Guan Cun Beijing 100080 China.

Signal transduction in early embryogenesis needs to be properly controlled. A new player involved in tuning down TGF β signaling has now been identified – new evidence that multi-layer control of signaling is essent... 详细信息

关键词： A new trick to tune down TGF signal

Structural Analysis and Identification of Cis-Elements of Rice osRACD Gene

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Acta Biochimica et Biophysica Sinica 2004年第3期36卷 191-198页

作者： Yun-Zhe XU, Rui-Lin YOU and Nai-Hu wu institute of genetics and {3. {4., chinese academy of Sciences, Beijing 100080, china 2College of Life Sciences, Peking University, Beijing 100871, china institute of genetics and developmental {3. Chinese Academy of Sciences College of Life Sciences Peking University

The osRACD gene correlated with fertility transformation in the photoperiod sensitive genic male sterile rice (PGMR), Nongken 58S, encoded a rice (Oryza sativa L. ssp. japonica) small GTPase belonging to the Rac/Rho family. Inverse PCR was performed to amplify a fragment about 1.4.kb in 5' upstream region of the osRACD promoter. Deletion mutation and gel mobility shift assay characterized two fragments (–799 to –686 nt, and –686 to –4.0} nt) in the osRACD promoter that could be involved in its transcriptional regulation. When these two deletion fragments were used as probe respectively, a retarded band appeared in the nuclear extracts of fertile 58S rice under short day (58S-SD). Whereas no retarded band was shown in the nuclear extracts of sterile 58S rice under long day (58S-LD). Competition assay indicated that the factors in the retarded bands binding to these two fragments were the same trans-acting factor (termed rice factor, RF). The binding affinity of RF was affected by phosphorylation and was higher in SD-growth rice than that of LD-growth rice.

关键词： photoperiod-sensitive male genic sterile rice osRACD gene photoperiod fertility transformation cis-element

Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development

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Cell Research 2004年第1期14卷 8-15页

作者： Yu Xin HU~*, Yong Hong WANG~*, Xin fang LIU, Jia Yang LI~(**) institute of genetics and {3. {4., chinese academy of Sciences, Beijing 100101, china. Li JY Chinese Acad Sci Inst Genet & Dev Biol Beijing 100101 Peoples R China

RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants, but its role in plant growth and development remains unknown. Using cDNA array, we found that transcription of RAV1 is downregulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein, and does not require the functional BRI1, suggesting that this regulation might be through a new BR signaling pathway. Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development, and the underexpression causes an earlier flowering phenotype, implying that RAV1 may function as a negative regulatory component of growth and development.

关键词： RAV1 Brassinosteroid signal transduction Arabidopsis thaliana.

Effects of space environment on biological characters of tissue cultured rose seedlings

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Progress in Natural Science:Materials International 2005年第5期15卷 414-418页

作者： XUE Huai, LIU Min*, LU Jinying, PAN Yi and zhang Chunhua(institute of genetics and {3. {4., chinese academy of Sciences, Beijing 100101, china) institute of genetics and developmental {3. Chinese Academy of Sciences Beijing 100101 China

Tissue cultured rose seedlings were carried into space by SHENZHOU-4.spacecraft and then used as the experimental material to investigate effects of the space environmental conditions on morphology, cytology, physiology and molecular biology of the seedlings. After loaded on the space flight, the plant’s height, number of leaves, and fresh weight per seedling were all increased significantly compared to the ground controls. The content of chlorophyll was basically unchanged. In some cells, the ultrastructural changes involved twist, contraction and deformation of cell wall, curvature and loose arrangement of lamellae of some chloroplasts, and a significant increase in number of starch grains per chloroplast. In addition, the number of mitochondria increased, but some mitochondrial outer membrane broke, and some mitochondrial cristae disappeared. The activities of the defense enzymes, such as superoxide dismutase, peroxidase and catalyse, in rose leaves increased and the content of malondialdehyde decreased. In the RAPD analysis with 4. 10-mer primers, 3. primers generated 14. DNA bands from both of the space flight treated seedlings and the ground controls, and five primers amplified polymorphic products. The rate of DNA variation was 6.3.%.

关键词： space environmental conditions rose biological characters tissue cultured seedlings.

Structure and function of Rac genes in higher plants

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Progress in Natural Science:Materials International 2003年第10期 1-9页

作者： LUO Min and wu naihu(institute of {3. and {4. biology, chinese academy of Sciences, Beijing 100080, china) institute of genetics and developmental {3. Chinese Academy of Sciences Beijing 100080 China

As the sole ubiquitous signal GTP binding protein in higher plants, Rac genes act as pivotal molecular switches and participate in regulations of many life activities, such as cell morphogenesis and polarity growth, programmed cell death, production of H 2O 2, cell differentiation, and hormone reaction. Based on our work on rice Rac genes, this paper summarizes the researches on Rac genes in higher plant of the last ten years. It will help us to understand the relation between the signal tranduction and the biological functions of plant Rac.

关键词： Rac family higher plant gene distribution structure and function.

Cloning and roles of goldfish maternal factor β-Catenin cDNA in embryonic development

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chinese Science Bulletin 2004年第7期49卷 683-688页

作者： zhang Jingpu1,2,WANG Weixian1 & ZHU Shaoxia1 1.institute of genetics and {3. {4.,chinese academy of Sciences,Beijing 100080,china 2.The Graduate School of the chinese academy of Sciences,Beijing 10003.,china Correspondence should be addressed to zhang Jingpu institute of genetics and developmental {3. Chinese Academy of Sciences Beijing 100080 China The Graduate School of the chinese academy of Sciences Beijing 100039 China

Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. b-Catenin, an important maternal factor in early embryo-genesis, has been analyzed in its expression pattern and func-tions in this paper. We have cloned goldfish b-Catenin cDNA gene and compared it with zebrafish b-Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93. (2227/23.4.bp) and 98.5% (768/780 aa) respectively. The expression pattern of b-Catenin by in situ hybridization and the roles of b-Catenin on embryonic de-velopment by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that b-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of b-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposte-rior axes, and leads embryos to die before larva get hatched.

关键词：胚胎发育母体因子金鱼基因克隆氨基酸序列 cDNA 细胞质细胞核

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Genetic variations of glycinin subunit genes among cultivated and wild type soybean species

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Progress in Natural Science:Materials International 2008年第1期18卷 33-41页

作者： Chunmei Wang, Xiaolei wu, Faxing Jia, Jinsong zhang *, Shouyi Chen * National Key Laboratory of Plant Genomics, institute of {3. and {4. biology, chinese academy of Sciences, Beijing 100101, china National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Glycinin is a predominant storage protein in most soybean accessions. It is a hexamer constituted by five major subunits, which can be classified into two groups. Group I contains G1, G2 and G3. and Group II contains G4.and G5. The genes encoding these subunits have been designated from Gy1 to Gy5, respectively. In the present study, Gy1 genomic fragments were cloned from wild accessions of sub- genera Glycine glycine, Glycine soja and a cultivar of Glycine max. Their sequences and the deduced amino acid sequences were com- pared. The residues critical for assembling of G1 subunits from the wild perennial accession were conservative. The Gy4.fragments were cloned from two wild perennial accessions and compared with that from subgenus Soja. The intron 3.of Gy4.had abundant vari- ations between the subgenera G. soja and G. glycine as well as within the subgenus G. glycine. Abundant variations existed in the dis- ordered regions 3.and 4.of G4.subunits from two wild perennial accessions. The genomic organization of glycinin genes was analyzed in 19 accessions from subgenera Soja and Glycine. The hybridization patterns were identical among the accessions of subgenus Soja. On the contrary, abundant polymorphisms existed between the accessions from subgenus Glycine. These results indicated that gly- cinin genes have high degree of conservation within subgenus Soja but more variations within subgenus Glycine.

关键词： Genetic variation Glycinin subunit genes Soybean

Plant gene co-expression defines the biosynthetic pathway of neuroactive alkaloids

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Molecular Plant 2024年第3期17卷 372-374页

作者： fang Liu' Alisdair R.Fernie Youjun zhang Key Laboratory of Seed Innovation Institute of Genetics and Developmental BiologyChinese Academy of Sciences100101 BeijingChina Max Planck institute of Molecular Plant Physiology Am Muhlenberg 114476 Potsdam-GolmGermany Center for Plant Systems biology and Biotechnology 4000 PlovdivBulgaria University of chinese academy of Sciences 100049 BeijingChina

Transcriptomes comprise gene co-expression clustersor modules,showcasing distinct patterns of coordinated expression among groups of genes across independent biological samples(Serin et al.,2016).The"guilt-by-association"hypothesis suggests the functional importance of these clusters;for example,co-expressed genes associated with metabolism typically contain genes encoding several enzymes associated with the same pathways.

关键词： metabolism alkaloids