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Impeded Carbohydrate Metabolism in Rice Plants under Submergence Stress

Rice science 2011年第2期18卷 116-126页

作者： Malay Kumar ADAK Nirmalya GHOSH Dilip Kumar DASgupta sudha gupta Plant Physiology and Plant Molecular Biology Research Unit Department of Botany University of Kalyani Department of Crop Physiology University of Calcutta

The detrimental effects of submergence on physiological performances of some rice varieties with special references to carbohydrate metabolisms and their allied enzymes during post-flowering stages have been documented and clarified in the present investigation. It was found that photosynthetic rate and concomitant translocation of sugars into the panicles were both related to the yield. The detrimental effects of the complete submergence were recorded in generation of sucrose, starch, sucrose phosphate synthase and phosphorylase activity in the developing panicles of the plants as compared to those under normal or control （i.e. non-submerged） condition. The accumulation of starch was significantly lower in plants under submergence and that was correlated with ADP-glucose pyrophosphorylase activity. Photosynthetic rate was most affected under submergence in varying days of post-flowering and was also related to the down regulation of Ribulose bisphosphate carboxylase activity. However, under normal or control condition, there recorded a steady maintenance of photosynthetic rate at the post-flowering stages and significantly higher values of Ribulose bisphosphate carboxylase activity. Still, photosynthetic rate of the plants under both control and submerged conditions had hardly any significant correlation with sugar accumulation and other enzymes of carbohydrate metabolism like invertase with grain yield. Finally, plants under submergence suffered significant loss of yield by poor grain filling which was related to impeded carbohydrate metabolism in the tissues. It is evident that loss of yield under submergence is attributed both by lower sink size or sink capacity （number of panicles, in this case） as well as subdued carbohydrate metabolism in plants and its subsequent partitioning into the grains.

关键词： photosynthesis sucrose starch phosphorylase grain yield rice submergence

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Amperometric Biosensor:Increased Sensitivity Using Enzyme Nanoparticles

Amperometric Biosensor:Increased Sensitivity Using Enzyme Na...

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International Conference on Nanotechnology and Biosensors（ICNB 2010）

作者： Shikha Sharma Aditi Shrivastav Nidhi gupta sudha Srivastava Dept.of Biotechnology Jaypee Institute of Information Technology

Here we present a comparative study of amperometric biosensor developed by using nanoparticles of Glucose oxidase enzyme itself （GOx np） with that of enzyme immobilized on to gold nanoparticles （Au np-GOx）.The biosensor developed by immobilizing the GOx np （45-70 nm in size） directly onto the *** could detect glucose concentrations as low as 1 mM while the one with Au np-GOx （Au np 10-16 nm in size） showed a detection limit up to 5 mM *** response showed linearity up to 35 mM in both the *** the best of our knowledge the glucose biosensors prepared using various metal nanoparticles,Au,Ni,Ag （as reported in literature） showed a linear range from 2.7-30 mM glucose *** are in the process of optimizing the immobilization methods and functionalization of the enzyme nanoparticles so as to have a wider temperature and pH stability of the *** feel the direct immobilization of enzyme nanoparticles,with proper choice of functionalization seems to be a promising strategy for biosensor development with higher sensitivities.

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A 10 Years Comparative Study to Assess Trends in Seroprevalence of Transfusion Transmitted Infections among Blood Donors at Gwalior, India

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Open Journal of Blood Diseases 2014年第2期4卷 24-32页

作者： Dharmesh Chandra Sharma Sunita Rai Savita Bharat sudha Iyenger Shruti gupta Bharat Jain Department of Microbiology Gajra Raja Medical College Gwalior India Department of Pathology Blood Bank Gajra Raja Medical College Gwalior India

Introduction: Transfusion Transmitted Infections (TTIs) threaten safety of the recipients and the community as a whole and are the subject of real concern worldwide. Aims and Objectives: To know the prevalence of transfusion transmitted infections amongst the blood donors, to evaluate the changing trends of TTIs and to compare these observations within the study as well as with the other relevant studies. Place and Duration of Study: This study was carried out at Blood Bank, Department of Pathology, Gajra Raja Medical College, Gwalior, India, from January 2004 to December 2013 (ten years). Materials and Methods: In this study 122,006 voluntary and replacement donations were screened for TTIs;HIV, HBV, HCV, Syphilis, Malaria and their seroprevalence was calculated. Further study was divided in Group “A” (from 2004 to 2008) and Group “B” (from 2009 to 2013) to compare the results. Results: Out of total 122,006 blood units collected, 79,750 (65.3%) were voluntary and 42,256 (34.7%) were replacement donors. The seropositivity of TTIs in the entire study, in group “A” and in Group “B” was 3.26% (3985/122,006) (p = 0.000005), 2.25% (1238/54,874) (p = 0.000005) and 4.09% (2747/67,123) (p = 0.000005) respectively. In Group “A” and “B” seroprevalence of HIV, HBV, HCV, Syphilis and Malaria was 0.29%, 1.16%, 0.61%, 0.06%, 0.11% and 0.13%, 3.15%, 0.24%, 0.17%, 0.03% respectively. Conclusion: Our study concluded that there was significant increase in seroprevalence of HBV and syphilis whereas decreasing pattern in HIV, HCV and Malaria was observed in last five years as compared to previous five years among the blood donors.

关键词： Transfusion Transmitted Infections (TTIs) Blood Donor (BD) Human Immunodeficiency Virus (HIV) Hepatitis B Virus (HBV) Hepatitis C Virus (HCV) Syphilis and Malaria

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Journey of DNA Evidence in Legal Arena:An Insight on Its Legal Perspective Worldwide and Highlight on Admissibility in India

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Journal of Forensic Science and Medicine 2016年第2期2卷 102-106页

作者： Ramakant gupta Swati gupta Manju gupta Department of Oral and Maxillofacial Surgery Sudha Rustagi College of Dental Sciences and Research1Department of Oral and Maxillofacial PathologyLala Diwan Chand Hospital Department of Psychology Dr.Jatinder Gupta’s Gupta Clinic and DiagnosticsFaridabadHaryanaIndia

DNA profiling is one of the powerful breakthroughs in *** specialized technique has made the identification of an individual possible even by a tiny shred of tissue or drop of blood thus,has strongly revolutionized various criminal ***,paternity,and murder cases are the type of criminal cases commonly solved by the use of this *** has been recently introduced to forensic odontology and is also used *** this is a powerful and reliable scientific technique but its forensic use is a major contribution to the debate on law *** application of DNA profiling in the criminal justice system,i.e.,the admissibility of DNA evidence in court of law is an important issue which is being faced by the courts and forensic experts worldwide ***,a proper legal outlook is required while dealing with this kind of scientific ***,this review intends to make forensic experts/odontologists aware about the admissibility of DNA evidence in court,with a highlight on the laws related to the admissibility of evidence worldwide,having a special focus on the laws related to admissibility of evidence in Indian judicial *** this review,the literature was overviewed from articles on DNA evidence and admissibility retrieved by searches on electronic databases such as Google,PubMed,and EMBASE from 1975 through July 2015.

关键词： Admissibility DNA evidence DNA profiling forensic odontology

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Evaluation of the Secretome Profile and Functional Characteristics of Human Bone Marrow Mesenchymal Stromal Cells-Derived Conditioned Medium Suggest Potential for Skin Rejuvenation

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Journal of Cosmetics, Dermatological Sciences and Applications 2017年第1期7卷 99-117页

作者： sudha Balasubramanian Charan Thej Ankita Walvekar Priyanka Swamynathan Pawan K. gupta Raviraja N. Seetharam Anish S. Majumdar Stempeutics Research Pvt. Ltd. Bangalore India Manipal University Manipal India

Background/Purpose: Multipotent bone marrow-derived mesenchymal stromal cells (BMMSC) have been shown to possess the potential for tissue regeneration. The application of mesenchymal stromal cells (MSC)-derived growth factors and cytokines (GF/CKs) has been implicated for the repair and regeneration of the damaged skin that occurs due to aging and exposure to environmental stress factors. Methods: We have used both qualitative and quantitative measurements of the GF/CKs from the conditioned medium (CM) of a pooled population of BMMSC by antibody array analysis as well as by enzyme-linked immunosorbent assay (ELISA). Furthermore, the CM was also used in a variety of in vitro biological assays to measure its protective properties in human skin fibroblasts. Results: We have characterized the secretome of BMMSC by analyzing the composition of the CM using a forty-one growth factor array system. Thirteen of these GF/CK/extra cellular matrix (ECM)/ matrix metalloproteinases (MMP)-inhibitors in the CM were quantified owing to their involvement in skin repair cascade. In addition, we report that the BMMSC-CM was also able to protect dermal fibroblasts against tert-Butyl hydro peroxide (tbOH) induced oxidative stress and ultra violet B (UV-B) radiation induced cell damage. Conclusion: Based on the data presented here, we propose that BMMSC-derived CM may have the potential to promote health and rejuvenation of the aging skin.

关键词： Bone Marrow-Derived MSC Conditioned Medium Secretome Oxidative Stress UV-B Damage

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