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No difference in high-magnification morphology and hyaluronic acid binding in the selection of euploid spermatozoa with intact DNA

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Asian Journal of Andrology 2013年第3期15卷 421-424页

作者： suchada mongkolchaipak Teraporn Vutyavanich Assisted Conception Center Phyathai Sriracha Hospital Cholburi 20110 Thailand ivision of Reproductive medicine Department of Obstetrics and Gynecology Faculty of Medicine Chiang Mai University Chiang Mai 50200 Thailand

In this study, we compared conventional sperm selection with high-magnification morphology based on the motile sperm organellar morphology examination （mSOmE） criteria, and hyaluronic acid （HA） binding for sperm chromosome aneuploidy and DNA fragmentation rates. Semen from 50 severe male factor cases was processed through density gradient centrifugation, and subjected to sperm selection by using the conventional method （control）, high magnification at x6650 or HA binding. Aneuploidy was detected by fluorescence in situ hybridization with probes for chromosomes 13, 18, 21, X and Y, and DNA fragmentation by the terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） method. Spermatozoa selected under high-magnification had a lower DNA fragmentation rate （2.6% vs. 1.7%; P=0.032）, with no significant difference in aneuploidy rate （0.8% vs0.7%; P=0.583）, than those selected by the HA binding method. Spermatozoa selected by both methods had much lower aneuploidy and DNA fragmentation rate than the controls （7% aneuploidy and 26.8% DNA fragmentation rates, respectively）. In the high-magnification group, the aneuploidy rate was lower when the best spermatozoa were selected than when only the second-best spermatozoa were available for selection, but the DNA fragmentation rate was not different. In conclusion, sperm selection under high magnification was more effective than under HA binding in selecting spermatozoa with low DNA fragmentation rate, but the small difference （0.9%） might not be clinically meaningful. Both methods were better than the conventional method of sperm selection.

关键词： aneuploidy DNA fragmentation hyaluronic acid binding intracytoplasmic sperm injection (ICSI) intracytoplasmic mor-phologically selected sperm injection (ImSI) motile sperm organellar morphology examination physiologic intracytoplasmic sperminjection

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Crystal Structure of 2-(2-Carboxy-4-hydroxy-6-methoxyphenoxy)-3,5-dichloro-6-hydroxy-4-methylbenzoic Acid 1-methyl Ester

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Chinese Journal of Structural Chemistry 2013年第11期32卷 1742-1748页

作者： suchada Jongrungruangchok Thammarat Aree Sanya Sureram Prasat Kittakoop Department of Pharmaceutical Chemistry Faculty of PharmacyRangsit University Department of Chemistry Faculty of ScienceChulalongkorn University Chulabhorn Research Institute Vibhavadi-Rangsit Highway Chulabhorn Graduate Institute and the Center for Environmental Health Toxicology and Management of ChemicalsChemical Biology ProgramVibhavadi-Rangsit Highway

The title compound, neogeodin hydrate （C17H14C1208, CAS： 94540-50-8）, was derived from marine fungus Aspergilhts terreus CRIm301. It was unequivocally characterized by IR, NmR spectroscopies, and single-crystal X-ray crystallography and tested for various biological activities. Neogeodin hydrate crystallizes in the triclinic space group P1 with a = 8.1159（5） A, b = 8.2472（4） A, c= 14.1278（7） A, a = 81.448（2）°, β = 84.860（2）°, γ= 70.400（2）°, V = 880.13（8） A3; Z = 2. It comprises a diphenyl ether, asterric acid skeleton and dichloro substituents. The methoxyphenoxy rings of the inversely related molecules form a ribbon-like structure that is stabilized by O-H...O hydrogen bonds through the doubly disordered carboxyl groups and by C-H...O interactions, generating the same R22（8） ring motif. The chlorinated methylbenzoate rings, making mostly a right angle, link the parallel upper and lower ribbons via bifurcated O-H...O and C-H...O hydrogen bonds, yielding endless channels. The channels formed are further sustained by C-H...O and π...π interactions Neogeodin hydrate exhibits inhibition against superoxide anion radical formation in the xanthine/xanthine oxidase （XXO） assay, but has no aromatase inhibitory activity.

关键词： Aspergiilus terreus biological activity hydrogen bonds,marine natural products neogeodin hydrate

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mitigating microbiologically influenced corrosion of an oilfield biofilm consortium on carbon steel in enriched hydrotest fluid using 2,2-dibromo-3-nitrilopropionamide(DBNPA) enhanced by a 14-mer peptide

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Journal of materials Science & Technology 2020年第22期54卷 146-152页

作者： Di Wang mahmoud Ramadan Sith Kum seranee suchada Punpruk Tingyue Gu Department of Chemical&Biomolecular Engineering Institute for Corrosion and Multiphase TechnologyOhio UniversityAthens 45701USA PTT Exploration and Production Bangkok 10900Thailand

In the oil and gas industry,microbiologically influenced corrosion(mIC) is a major threat to hydrotest,a procedure which is required to certify whether a pipeline can be *** is frequently used as a hydrotest *** this bio film prevention lab study,an oilfield biofilm consortium was grown in an enriched artificial seawater anaerobically at 37℃ for 60 *** combination of 100 ppm(w/w) 2,2-dibromo-3-nitrilopropionamide(DBNPA)+100 nm(180 ppb) Peptide A(a biofilm dispersal agent) led to extra SRB(sulfate reducing bacteria),APB(acid producing bacteria) and GHB(general heterotrophic bacteria) sessile cell count reductions of 0.9-log,0.8-log and 0.6-log,respectively,compared with the outcome obtained by using 100 ppm DBNPA *** Peptide Aenhancement also led to extra reductions of 44 % in weight loss,43 % in maximum pit depth,and 54 % in corrosion current density.

关键词： Hydrotest microbiologically influenced corrosion Anti-biofilm peptide Biocide enhancer 2 2-dibromo-3-nitrilopropionamide(DBNPA)

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“Corrosion-resistant”chromium steels for oil and gas pipelines can suffer from very severe pitting corrosion by a sulfate-reducing bacterium

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Journal of materials Science & Technology 2024年第7期174卷 23-29页

作者： Lingjun Xu Pruch Kijkla Sith Kumseranee suchada Punpruk Tingyue Gu Department of Chemical&Biomolecular Engineering Institute for Corrosion and Multiphase TechnologyOhio UniversityAthens 45701USA PTT Exploration and Production Bangkok 10900Thailand

*** CO_(2)corrosion of pipelines is one of the major threats in the oil and gas industry[1].Chromium steels(CrSs)and more expen-sive stainless steels(SSs)can mitigate CO_(2)corrosion[2,3].A higher content of Cr results in greater uniform corrosion resistance to CO_(2),which is generally attributed to the enrichment of Cr in the corro-sion product film[4,5].The film,which mainly consists of FeCO_(3),Fe(OH)_(3),Cr(OH)_(3),and Cr_(2)O_(3),forms a passive layer on the metal surface,and the enrichment of Cr in this layer helps block corro-sive anions from contacting with the steel surface[6-8].

关键词： corrosion pitting steel

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Effect of high-pressure homogenization on stability of emulsions containing zein and pectin

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Asian Journal of Pharmaceutical Sciences 2017年第1期12卷 21-27页

作者： maneerat Juttulapa suchada Piriyaprasarth Hirofumi Takeuchi Pornsak Sriamornsak Department of Pharmaceutical Technology Faculty of PharmacySilpakorn UniversityNakhon Pathom 73000Thailand Pharmaceutical Biopolymer Group(PBiG) Faculty of PharmacySilpakorn UniversityNakhon Pathom 73000Thailand Laboratory of Pharmaceutical Engineering Gifu Pharmaceutical University1-25-4 Daigaku-NishiGifu 501-1196Japan

The aim of this study was to investigate the effect of high-pressure homogenization on the droplet size and physical stability of different formulations of pectin–zein stabilized rice bran oil emulsions. The obtained emulsions, both before and after passing through highpressure homogenizer, were subjected to stability test under environmental stress conditions,that is, temperature cycling at 4 °C/40 °C for 6 cycles and centrifugal test at 3000 rpm for 10 min. Applying high-pressure homogenization after mechanical homogenization caused only a small additional decrease in emulsion droplet size. The droplet size of emulsions was influenced by the type of pectin used;emulsions using high methoxy pectin(HmP) were smaller than that using low methoxy pectin(LmP). This is due to a greater emulsifying property of HmP than LmP. The emulsions stabilized by HmP–zein showed good physical stability with lower percent creaming index than those using LmP, both before and after passing through high-pressure homogenizer. The stability of emulsions after passing through high-pressure homogenizer was slightly higher when using higher zein concentration, resulting from stronger pectin–zein complexes that could rearrange and adsorb onto the emulsion droplets.

关键词： Pectin Zein Emulsions High-pressure homogenization Stability

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A new self-emulsifying formulation of mefenamic acid with enhanced drug dissolution

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Asian Journal of Pharmaceutical Sciences 2015年第2期10卷 121-127页

作者： Pornsak Sriamornsak Sontaya Limmatvapirat suchada Piriyaprasarth Punyanutch mansukmanee Zongkang Huang Department of Pharmaceutical Technology Faculty of PharmacySilpakorn UniversityNakhon Pathom 73000Thailand Pharmaceutical Biopolymer Group(PBiG) Faculty of PharmacySilpakorn UniversityNakhon Pathom 73000Thailand

To enhance the dissolution of poorly soluble mefenamic acid,self-emulsifying formulation(SEF),composing of oil,surfactant and co-surfactant,was *** the oils and surfactants studied,Imwitor■ 742,Tween■ 60,Cremophore■ EL and Transcutol■ HP were selected as they showed maximal solubility to mefenamic *** ternary phase diagram was constructed to find optimal concentration that provided the highest drug *** droplet size after dispersion and drug dissolution of selected formulations were *** results showed that the formulation containing Imwitor■ 742,Tween■ 60 and Transcutol■ HP(10:30:60)can encapsulate high amount of mefenamic *** dissolution study demonstrated that,in the medium containing surfactant,nearly 100% of mefenamic acid were dissolved from SEF within 5 min while 80% of drugs were dissolved from the commercial product in 45 *** phosphate buffer(without surfactant),80% of drug were dissolved from the developed SEF within 5 min while only about 13% of drug were dissolved in 45 min,from the commercial *** results suggested that the SEF can enhance the dissolution of poorly soluble drug and has a potential to enhance drug absorption and improve bioavailability of drug.

关键词： Self-emulsifying formulation Poorly water-soluble drug mefenamic acid Drug dissolution

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Synthesis and Crystal Structure of Dinuclear Cadmium Complex [Cd_2(phen)_4(fca)_2](ClO_4)_2(H_2O)_2

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Chinese Journal of Structural Chemistry 2003年第5期22卷 558-562页

作者： ZHANGZe WUJie-Ying TIANYu-Peng DUPeng LIUQing-Liang FUNHoongKun C.suchada YANGJia-Xiang DepartmentofChemistry UniversityofScienceandTechnologyofChinaHefei230026China DepartmentofChemistry AnhuiUniversityHefei230039China StateKeyLaboratoryofCoordinationChemistry NanjingUniversityNanjing210093China X-rayCrystallographyUnit SchoolofPhysicsUniversitySainsMalaysia11800USMPenangMalaysia

A novel complex [Cd2(phen)4(fca)2](ClO4)2(H2O)2 (fca = dianion of 3-ferrocenyl- 2-crotonic acid, phen = 1,10-phenanthroline) has been synthesized and characterized by X-ray single-crystal structure analysis. It crystallizes in triclinic system, space group P?with a = 11.6297(5), b = 12.5165(5), c = 13.8986(6) ? a = 63.345(1), b = 87.747(1), g = 71.862(1), V = 1706.20(1) ?, Z = 1, Dc = 1.673 g/cm3, F(000) = 868 and m(moKa) = 1.185 mm-1 (l = 0.71073 ). R = 0.0800 and wR = 0.1793 for 7653 observed reflections (I > 2s(I)). The structure consists of a dinuclear cation [Cd2(phen)4(fca)2]2+, two discrete perchlorate anions and two water molecules. The cationic part of the complex shows a tetrametallic core in which two fca groups act as the (O, O) bridging ligands between two cadmium(Ⅱ) ions with a distorted octahedral environment. The Cd…Cd intradimer distance is 4.128 ?

关键词： crystal structure cadmium complex ferrocene derivative

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Tafel scan schemes for microbiologically influenced corrosion of carbon steel and stainless steel

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Journal of materials Science & Technology 2022年第35期130卷 193-197页

作者： Di Wang Pruch Kijkla mazen A.Saleh Sith Kumseranee suchada Punpruk Tingyue Gu Shenyang National Lab for materials Science Northeastern UniversityShenyang 110819China Department of Chemical and Biomolecular EngineeringInstitute for Corrosion and Multiphase TechnologyOhio UniversityAthens 45701United States Department of Chemical and Biomolecular Engineering Institute for Corrosion and Multiphase TechnologyOhio UniversityAthens 45701United States PTT Exploration and ProductionBangkok 10900Thailand Research and Development Center Saudi Arabian Oil CompanyDhahran 31311Saudi Arabia PTT Exploration and Production Bangkok 10900Thailand Department of Chemical and Biomolecular Engineering Institute for Corrosion and Multiphase TechnologyOhio UniversityAthens 45701United States

*** microbial biofilms cause microbiologically influenced corrosion(mIC),which is one of the operational risks that can lead to equipment failures in the oil and gas industry and other industries [1-4].mitigation measures such as biocide treatment and corrosion monitoring programs are implemented to ensure vital equipment integrity [5,6].The mechanism investigations in mIC can assist case analyses,field detections and biocide treatment strategies [7-9].

关键词： corrosion steel treatment

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Synthesis, Crystal Structures and Properties of Tetrametallic Complexes： [m2（phen）4（FCA）2]（ClO4）2·（H2O）2 （m=Zn or Co, phen=1,10-phenanthroline, FCA-anion of 3-ferrocenyl-2-crotonic acid）

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Chinese Journal of Chemistry 2005年第5期23卷 533-538页

作者：杨家祥胡张军张泽田玉鹏刘清亮 CHANTRAPROmmA,suchada FUN,Hoong-Kun DepartmentofChemistry AnhuiUniversityHefeiAnhui230039China DepartmentofChemistry UniversityofScienceandTechnologyofChinaHefeiAnhui230026China DepartmentofChemistry AnhuiUniversityHefeiAnhui230039China//StateKeyLaboratoryofCoordinationChemistryNanjingUniversityNanjingJiangsu210093China X-rayCrystallographyUnit SchoolofPhysicsUniversityofSainsMalaysia11800USMPenangMalaysia

Two new complexes [Zn2(phen)4(FCA)2](ClO4)2·(H2O)2 (1) and [Co2(phen)4 (FCA)2](ClO4)2·(H2O)2 (2) (FCA=anion of 3-ferrocenyl-2-crotonic acid, phen=1,10-phenanthroline) have been synthesized, and characterized by elemental analysis, IR, UV-Vis spectra, thermal analyses, and single-crystal X-ray diffraction. Two m(II) (m=Zn or Co) ions are bridged by two FCA anions with syn-anti bridging ligands, leading to dimeric cores, [m2(phen)4(FCA)2]2+, and each m(II) ion is six-coordinated in a distorted octahedral geometry by two chelate phen ligands and two μ2-carboxylate oxygen atoms from two FCA groups. The m(II)…m(II) intradimer distances are 0.4391 and 0.4462 nm in 1 and 2, respectively. Electrochemical properties of the complexes have been discussed.

关键词： assembly crystal structure ferrocene carboxylate-bridged complex electrochemistry

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Preparation and physical properties of itraconazole-loaded nanoemulsions using pineapple starch as co-emulsifier

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 110-111页

作者： Su Su Latt Kanit Boontara Tanapourn Teeraprasatkul Warisa Yangngam Vipaluk Patomchaiviwat Pornsak Sriamornsak suchada Piriyaprasarth Department of Pharmaceutical Technology Faculty of PharmacySilpakorn UniversityNakhon Pathom 73000Thailand Pharmaceutical Biopolymer Group(PBiG) Faculty of PharmacySilpakorn UniversityNakhon Pathom 73000Thailand

Pineapple plant(Ananascomosus ***.)is a leading edible member of the Bromeliaceae family[1].Nanoemulsions containing itraconazole,a poorly water-soluble drug using pectin as a polymeric emulsifier,are currently under investigation[2].The physicochemical and rheological properties as well as structural characterizations of cassava and mungbean starches have been reported[3].However,the application of pineapple starch as co-emulsifier of pharmaceutical excipients for pharmaceutical industry has not been investigated.

关键词： Pineapple starch Pectin Co-emulsifier Polysorbate 80 Itraconazole Nanoemulsions

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