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International Journal of Ophthalmology(English edition) 2011年第3期4卷 239-242页

作者： Yun Wang, su-ping cai Ophthalmic Laboratories & Department of Ophthalmology State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Second Affiliated hospital of Nanhua University 421001 Hunan Province China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou Province 510060 China

AIM: To determine the effects of a low dose latrunculin (LAT)-A on dexamethasone (Dex)-induced upregulation of extracellular matrix proteins fibronectin (FN) in cultured human trabecular meshwork (HTM) cells. METHODS: HTM cells were cultured to confluent and incubated with 0.4 mu mol/L Dex and/or 0.05 mu mol/L LAT-A. FN expression in HTM cells was evaluated by Western blot and immunofluorescence microscopy. REsuLTS: Dex up-regulated FN production in HTM cells, failed to do so when co-incubated with LAT-A. LAT-A decreased production of FN in cultured HTM cells. CONCLUSION: This study indicated that LAT-A may modulate the expression of fibronectin in trabecular meshwork to achieve treatment for steroids and other types of glaucoma. It has an important prospect as an intraocular pressure-lowering drug.

关键词： latrunculin-A dexamethasone human trabecular meshwork cells fibronectin

Arg124Cys mutation of the TGFBI gene in a Chinese pedigree of Reis-Bücklers corneal dystrophy

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International Journal of Ophthalmology(English edition) 2011年第3期4卷 235-238页

作者： Qiao-Na Yang, su-ping cai Ophthalmic Laboratories & Department of Ophthalmology West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Ophthalmology the Affiliated Hospital of Vocational Technology Institute of Yongzhou Hunan Province China

AIM: To analyze mutations in transforming growth factor beta-induced (TGFBI) gene in a Chinese pedigree with Reis-Bücklers corneal dystrophy (RBCD,also known as GCD3).METHODS: In a five-generation Chinese family,eight members were identified with RBCD and the rest were *** members of the family underwent complete ophthalmologic *** of TGFBI were amplified by polymerase chain reaction,sequenced,and compared with a reference ***: A single heterozygous C>T (R124C) point mutation was found in exon 4 of TGFBI in all the affected members of the pedigree,but not in the unaffected ***: R124C which was a known mutation for lattice corneal dystrophy type I,segregated with the RBCD in this *** elucidated the correlation between genotype and phenotype in a Chinese family of RBCD.

关键词： Reis-Bücklers corneal dystrophy molecular genetics mutation TGFBI R124C

A mutated CRYGD associated with congenital coralliform cataracts in two Chinese pedigrees

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International Journal of Ophthalmology(English edition) 2021年第6期14卷 800-804页

作者： su-ping cai Lan Lu Xi-Zhen Wang Yun Wang Fen He Ning Fan Jing-Ning Weng Jun-Hua Zhang Xu-Yang Liu Shenzhen Key Laboratory of Ophthalmology Shenzhen Eye HospitalJinan UniversityShenzhen 518000Guangdong ProvinceChina Department of Ophthalmology Fujian Medical University Union HospitalFujian Medical University College of Medical Technology and EngineeringFuzhou 350001Fujian ProvinceChina Xiamen Eye Center Xiamen UniversityXiamen 361100Fujian ProvinceChina Department of Ophthalmology Shenzhen People’s Hospitalthe 2^(nd)Clinical Medical CollegeJinan UniversityShenzhen 518020Guangdong ProvinceChina

AIM:To investigate the causal gene mutation and clinical characteristics for two Chinese families with autosomal dominant congenital coralliform ***:Two Chinese pedigrees with congenital cataract were *** ophthalmic examinations were performed on all patients and non-affected family *** blood samples were collected,and the genomic DNAs were *** coding regions of proband’s DNAs were analyzed with cataract gene *** identified mutation was amplified by polymerase chain reaction,and automated sequencing was performed in other members of two families to verify whether the mutated gene was co-segregated with the ***:Congenital coralliform cataract was inherited in an autosomal dominant mode in both *** each family,more than half of the family members were *** patients presented with severe visual impairment after birth as a result of bilateral symmetric coralliform lens *** exact the same defect in the same gene,a heterozygous mutation of c.70 C>A(p.P24 T)in exon 2 of γ Dcrystallin gene,was detected in both probands from each *** sequencing analysis demonstrated that the mutated CRYGD was co-segregated in these two ***:A c.70 C>A(p.P24 T)variant in CRYGD gene was reconfirmed to be the causal gene in two Chinese *** is known that mutated CRYGD caused most of the congenital coralliform cataracts,suggesting that the CRYGD gene is associated with coralliform congenital cataract.

关键词： congenital cataract mutation CRYGD gene autosomal dominant

Single nucleotide polymorphism of MYOC affected the severity of primary open angle glaucoma

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International Journal of Ophthalmology(English edition) 2013年第3期6卷 264-268页

作者： Xiao-Min Zhou Yan Yin Ning Fan Hong-Bo Cheng Xiao-Hong Li Yun Wang Wen-Han Yu su-ping cai Xu-Yang Liu Regenerative Medicine Research Center West China Hospital Sichuan University Ophthalmic Laboratories & Department of Ophthalmology the State Key Laboratory of Biotherapy West China Hospital Sichuan University Shenzhen Eye Hospital Jinan University

AIM: To detect the mutations in two candidate genes, myocilin (MYOC ) and cytochrome P450 1B1 (CYP1B1 ), in a Chinese family with primary open angle glaucoma (POAG). ·METHODS:Thefamilywascomposedofthreemembers, the parents and a daughter. All members of the family underwent complete ophthalmologic examinations. Exons of MYOC and CYP1B1 genes were screened for sequence alterations by polymerase chain reaction (PCR) and direct DNA sequencing. ·REsuLTS: The mother was the proband, she was diagnosed as POAG in both eyes. Her daughter was diagnosed as juvenile -onset POAG. The father was asymptomatic. One MYOC heterozygous mutation c.1150 G >A (D384N) in exon 3 was identified in the mother, another MYOC heterozygous variation c.1058 C>T (T353I) in exon 3 was identified in the father, and the daughter inherited both of the variations. Meanwhile, three single nucleotide polymorphisms (SNPs) in CYP1B1 gene were found in the family. ·CONCLUSION: The D384N mutation of MYOC has been reported as one of disease-causing mutations in POAG, whereas T353I variation of MYOC was thought as a high risk factor for POAG. The two variations of MYOC were first reported in one juvenile -onset POAG patient whopresented with more severe clinical manifestations, suggesting that T353I polymorphism of MYOC may be associated with the severity of POAG.

关键词： primary open angle glaucoma myocilin mutation D384N T353I

Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase

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International Journal of Ophthalmology(English edition) 2023年第8期16卷 1196-1209页

作者： Jun-Kai Tan Ying Xiao Guo Liu Long-Xiang Huang Wen-Hao Ma Yan Xia Xi-Zhen Wang Xian-Jun Zhu su-ping cai Xiao-Bing Wu Yun Wang Xu-Yang Liu Xiamen Eye Center Xiamen UniversityXiamen 361004Fujian ProvinceChina Department of Pathology Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610032Sichuan ProvinceChina Sichuan Provincial Key Laboratory for Human Disease Gene Study Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072Sichuan ProvinceChina The First Affiliated Hospital of Fujian Medical University Fuzhou 350004Fujian ProvinceChina Beijing FivePlus Molecular Medicine Institute Co. Ltd.Beijing 102600China Shenzhen Key Laboratory of Ophthalmology Shenzhen Eye HospitalSchool of OptometryJinan UniversityShenzhen 518040Guangdong ProvinceChina Department of Ophthalmology Shenzhen People’s Hospitalthe 2nd Clinical Medical CollegeJinan UniversityShenzhen 518020Guangdong ProvinceChina

AIM:To evaluate the potential of two trabecular meshwork(TM)-specific promoters,Chitinase 3-like 1(Ch3L1)and matrix gla protein(MGP),for improving specificity and safety in glaucoma gene therapy based on self-complementary AAV2(scAAV2)vector ***:An scAAV2 vector with C3 transferase(C3)as the reporter gene(scAAV2-C3)was *** scAAV2-C3 vectors were driven by Ch3L1(scAAV2-Ch3L1-C3),MGP(scAAV2-MGP-C3),enhanced MGP(scAAV2-eMGP-C3)and cytomegalovirus(scAAV2-CMV-C3),*** cultured primary human TM cells were treated with each vector at different multiplicities of *** in cell morphology were observed by phase contrast *** stress fibers and Rho GTPases/Rho-associated protein kinase pathway-related molecules were assessed by immunofluorescence staining,real-time quantitative polymerase chain reaction and Western *** vector was injected intracamerally into the one eye of each rat at low and high doses *** vivo green fluorescence was visualized by a Micron III Retinal Imaging *** pressure(IOP)was monitored using a rebound *** responses were evaluated by slit-lamp *** histopathology analysis was examined by hematoxylin and eosin ***:In TM cell culture studies,the vectormediated C3 expression induced morphologic changes,disruption of actin cytoskeleton and reduction of fibronectin expression in TM cells by inhibiting the Rho GTPases/Rhoassociated protein kinase signaling *** the same dose,these changes were significant in TM cells treated with scAAV2-CMV-C3 or scAAV2-Ch3L1-C3,but not in cells treated with scAAV2-eMGP-C3 or *** lowinjected dose,the IOP was significantly decreased in the scAAV2-Ch3L1-C3-injected eyes but not in scAAV2-MGPC3-injected and scAAV2-eMGP-C3-injected *** highinjected dose,significant IOP reduction was observed in the scAAV2-eMGP-C3-injected eyes but not in scAAV2-MGP-C3-injected *** t

关键词： self-complementary AAV2 chitinase 3-like 1 matrix gla protein trabecular meshwork C3 transferase

山西省繁峙县义兴寨金矿地质特征及成矿条件分析

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科技创新与生产力 2011年第1期 104-106页

作者：苏彩萍山西省地质勘查局二一一地质队山西忻州034000

义兴寨金矿床是五台山区具有代表性的矿床类型,该类型矿床规模大、品位好,具有很大的开发利用价值。对繁峙县义兴寨金矿的地质特征及成矿条件进行了分析,为今后该地区的找矿有重要的指导意义。

关键词：义兴寨金矿地质特征成矿条件

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繁峙黄草金矿地质特征及成因

太原科技 2010年第1期192卷 69-71页

作者：苏彩萍山西省地质勘查局二一一地质队山西忻州034000

介绍了黄草金矿的地质背景,包括地层和构造,并对矿床的地质特征、矿床成因及找矿标志进行了分析。

关键词：繁峙黄草金矿地质特征找矿标志

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代县滩上金银矿床类型成矿序列特征分析

太原科技 2009年第3期 65-67页

作者：苏彩萍山西省地质勘查局二一一地质队山西忻州034000

代县滩上一带是晋北地区主要的火山岩成矿区之一。探讨了代县滩上矿床地质特征,在充分研究前人已有成果的前提下,对区内各矿床的成矿规律进行了对比分析。

关键词：代县滩上金银矿床成矿类型成矿规律成矿序列

Experimental Tibetan monkey domestication and its application for intraocular pressure measurement

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Experimental Tibetan monkey domestication and its applicatio...

Porous Organic Cage‑Based Quasi‑Solid‑State Electrolyte with Cavity‑Induced Anion‑Trapping Effect for Long‑Life Lithium Metal Batteries

第十届中国实验动物科学年会

作者： Yong Yi Tao Zeng Liang Zhou su-ping cai Yan Yin Yun Wang Xu Cao Yue-Zhong Xu Hong-Xing Wang Xu-Yang Liu Institute of Laboratory Animals of Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Shenzhen Eye Hospital Jinan University Ophthalmic Laboratories & Department of Ophthalmology West China HospitalSichuan University

AIM:To train Tibetan monkey(Macaca thibetana) for intraocular pressure(IOP) measurement in conscious state and obtain normal IOP in conscious Tibetan Macaque. METHODS:The training was based on award-conditioned *** stimulation and human-animal interaction were used in this training. REsuLTS:Trained Tibetan monkeys calmly accepted IOP measurement by the TonoVetrebound tonometer without sedation or anesthesia and their IOP values were similar to other primates. CONCLUSION:Human-cultivated Thibetan monkeys are tamable,and can be used for biomedical research such as ophthalmic research without anesthesia.

关键词： Macaca thibetana domestication conscious intraocular pressure measurement

来源： cnki会议评论

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Nano-Micro Letters 2025年第2期17卷 376-386页

作者： Wei-Min Qin Zhongliang Li Wen‑Xia su Jia‑Min Hu Hanqin Zou Zhixuan Wu Zhiqin Ruan Yue‑Peng cai Kang Li Qifeng Zheng School of Chemistry South China Normal UniversityGuangzhou 510006People’s Republic of China Key Laboratory of Functional Metal‑Organic Compounds of Hunan Province College of Chemistry and Material ScienceHengyang Normal UniversityHengyang 421008People’s Republic of China Guangzhou Key Laboratory of Energy Conversion and Energy Storage Materials Guangzhou 510006People’s Republic of China

Porous organic cages(POCs)with permanent porosity and excellent host–guest property hold great potentials in regulating ion transport behavior,yet their feasibility as solid-state electrolytes has never been testified in a practical ***,we design and fabricate a quasi-solid-state electrolyte(QSSE)based on a POC to enable the stable operation of Li-metal batteries(LMBs).Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC,the resulting POC-based QSSE exhibits a high Li+transference number of 0.67 and a high ionic conductivity of 1.25×10^(−4) S cm^(−1) with a low activation energy of 0.17 *** allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 *** a proof of concept,the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85%capacity retention after 1000 ***,our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems,such as Na and K batteries.

关键词： Porous organic cage Cavity-induced anion-trapping Quasi-solid-state electrolyte Homogeneous Li+flux Lithium metal battery