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Virologica Sinica 2010年第1期25卷 45-51页

作者： Peng-fei WEI Yan-jun LUO Tian-xian LI Hua-lin WANG Zhi-hong HU Fu-chun ZHANG Yu-jiang ZHANG Fei DENG su-rong sun Xinjiang Key Laboratory of Biological Resources and Technology Xinjiang University Urumqi 830046 China Genetic Engineering College of Life Science and State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region Urumqi 830002 China

The Crimean-congo hemorrhagic fever virus(CCHFV)is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV *** this study,expression vectors carrying series truncated fragments of the NP(nucleocapsid protein)gene from the S fragment of CCHFV strain YL04057 were *** recombinant proteins were expressed in *** and purified for *** antigenic of the truncated fragments of NP was detected with a polyclonal serum(rabbit)and 2 monoclonal(mAbs)(14B7 and 43E5)against CCHFV by Western-blot analyses. The results showed that the three expressed constructs,which all contained the region 235AA to 305AA could be detected by mAbs polyclonal *** results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein.

关键词： Crimean-congo hemorrhagic fever virus (CCHFV) Expression Epitope Nucleocapsid protein (NP)

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我国高校体育产业协同创新发展研究

广州体育学院学报 2015年第2期35卷 117-119,122页

作者：孙素玲上海体育学院上海200438

协同创新是知识经济社会转型后提出的新的发展目标,而近年来我国高校体育产业协同创新发展在政府和市场发展的迫切需求下逐步发展,通过探究高校体育产业协同创新发展的迫切性,并思考其存在的诘症以及未来的发展路径。

关键词：高校体育产业协同创新

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一种基于特征的纺丝元件建模方法研究

计算机辅助设计与图形学学报 1999年第3期11卷 266-268页

作者：孙苏榕北京服装学院工业设计与自动化系

基于产品特征建模理论，通过对不同纺丝工艺和纺丝元件结构特点的分析，研究了以形状特征和纺丝工艺特征为对象的纺丝元件特征模型的定义方法，建立了一种面向工程设计的、又具有工程技术语义的纺丝元件特征模型，并在此基础上讨论了特... 详细信息

基于产品特征建模理论，通过对不同纺丝工艺和纺丝元件结构特点的分析，研究了以形状特征和纺丝工艺特征为对象的纺丝元件特征模型的定义方法，建立了一种面向工程设计的、又具有工程技术语义的纺丝元件特征模型，并在此基础上讨论了特征库中纺丝元件的定义．

关键词：纺丝元件特征建模化学纤维纺丝生产

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Web服务信誉度评估模型的研究

计算机工程与设计 2008年第9期29卷 2259-2261,2308页

作者：孙素云广东轻工职业技术学院计算机系广东广州510300

对Web服务的信誉度、信誉关系属性和信任类型进行了形式化的定义,并据此来评估Web服务的信誉行为,考虑到Web服务信誉度的不确定性,在引入信誉量化概念的基础上,对现有的UDDI规范进行扩展,提出了Web服务信誉度评估模型,并深入研究了Web... 详细信息

对Web服务的信誉度、信誉关系属性和信任类型进行了形式化的定义,并据此来评估Web服务的信誉行为,考虑到Web服务信誉度的不确定性,在引入信誉量化概念的基础上,对现有的UDDI规范进行扩展,提出了Web服务信誉度评估模型,并深入研究了Web服务的信誉度评估方法及模型的实现方案,与其它的Web服务信誉度模型相比,该模型通过使用客户反馈、主动监视的机制以及采用第三方权威机构评价Web服务的信誉度,从而保证了信誉度的公平性和有效性。

关键词： Web服务信誉度主动监视客户反馈评估模型

鸡传染性贫血病胶体金快速诊断试纸条的研制——鸡传染性贫血血清抗体的制备、纯化及检测

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安徽农业科学 2008年第12期36卷 4980-4981页

作者：孙素玲安徽科技学院安徽凤阳233100

[目的]为建立鸡传染性贫血病(CIA)诊断方法奠定基础。[方法]对15周龄SPF鸡进行4次免疫后,制备鸡传染性贫血病血清抗体并对其进行纯化,测定抗原最佳包被浓度和血清的最佳稀释度,研究纯化后血清抗体的蛋白含量和效价。[结果]阳性血清和阴... 详细信息

[目的]为建立鸡传染性贫血病(CIA)诊断方法奠定基础。[方法]对15周龄SPF鸡进行4次免疫后,制备鸡传染性贫血病血清抗体并对其进行纯化,测定抗原最佳包被浓度和血清的最佳稀释度,研究纯化后血清抗体的蛋白含量和效价。[结果]阳性血清和阴性血清的最佳稀释度为1∶100,病毒包被抗原最佳包被浓度为5μg/ml。血清抗体蛋白含量为13.2 mg/ml,血清抗体效价为1∶12 800。[结论]该研究为制备CIA胶体金快速诊断试纸条奠定了基础。

关键词：鸡传染性贫血病血清抗体胶体金试纸条

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立井箕斗闸门形式的探讨

煤矿机械 2006年第6期27卷 990-992页

作者：孙素娟河北能源职业技术学院河北唐山063000

一种箕斗是否好用,在很大程度上取决于它的闸门结构,所以分析和研究箕斗的闸门是很必要的。在大型箕斗设计中,这个问题更应慎重考虑。根据目前我国煤矿主井立井普遍使用的箕斗闸门,存在问题的分析及国外煤矿常用的几种箕斗的探讨,寻求... 详细信息

一种箕斗是否好用,在很大程度上取决于它的闸门结构,所以分析和研究箕斗的闸门是很必要的。在大型箕斗设计中,这个问题更应慎重考虑。根据目前我国煤矿主井立井普遍使用的箕斗闸门,存在问题的分析及国外煤矿常用的几种箕斗的探讨,寻求一种较好的新型结构的箕斗闸门,来满足我国煤矿不断生产的需求。

关键词：立井箕斗闸门形式探讨

Targeting a disintegrin and metalloprotease(ADAM)17-CD122 axis enhances CD8^(+)T cell effector differentiation and anti-tumor immunity

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Signal Transduction and Targeted Therapy 2024年第7期9卷 3097-3111页

作者： Lina sun Anjun Jiao Haiyan Liu Renyi Ding Ning Yuan Biao Yang Cangang Zhang Xiaoxuan Jia Gang Wang Yanhong su Dan Zhang Lin Shi Chenming sun Aijun Zhang Lianjun Zhang Baojun Zhang Department of Pathogenic Microbiology and Immunology School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anShaanxi 710061China Institute of Infection and Immunity Translational Medicine InstituteXi’an Jiaotong University Health Science CenterXi’anShaanxi 710061China Key Laboratory of Environment and Genes Related to Diseases Ministry of EducationXi’anShaanxi 710061China Xi’an Key Laboratory of Immune Related Diseases Xi’anShannxi 710061China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer InstituteXuzhou Medical UniversityXuzhouJiangsuChina Department of Pediatrics Qilu Hospital of Shandong UniversityJinanChina National Key Laboratory of Immunity and Inflammation Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina Key Laboratory of Synthetic Biology Regulatory Elements Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina

CD8^(+)T cell immune responses are regulated by multi-layer networks,while the post-translational regulation remains largely *** ectodomain shedding is an important post-translational process orchestrating receptor expression and signal transduction through proteolytic cleavage of membrane ***,by targeting the sheddase A Disintegrin and Metalloprotease(ADAM)17,we defined a post-translational regulatory mechanism mediated by the ectodomain shedding in CD8^(+)T *** and proteomic analysis revealed the involvement of post-translational regulation in CD8^(+)T cells.T cellspecific deletion of ADAM17 led to a dramatic increase in effector CD8^(+)T cell differentiation and enhanced cytolytic effects to eliminate pathogens and ***,ADAM17 regulated CD8^(+)T cells through cleavage of membrane ***17 inhibition led to elevated CD122 expression and enhanced response to IL-2 and IL-15 stimulation in both mouse and human CD8^(+)T ***,inhibition of ADAM17 in CD8^(+)T cells improved the efficacy of chimeric antigen receptor(CAR)T cells in solid *** findings reveal a critical post-translational regulation in CD8^(+)T cells,providing a potential therapeutic strategy of targeting ADAM17 for effective anti-tumor immunity.

关键词： immunity stimulation involvement

Application of Landscape Plants in the Huaxi National Urban Wetland Park

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Journal of Landscape Research 2017年第2期9卷 103-106,112页

作者： WANG Jian WANG Xiurong sun su TAN Li LIU Hongyu College of Forestry Guizhou University

The current situation of plants in Shilihe Beach of the Huaxi National Urban Wetland Park was investigated and analyzed. The results show that there are about 181 species of landscape plants in this park, of which arbors and shrubs are dominant. Various species of plants grow differently because of different types of artificial maintenance. The overall partition of the wetland park dose not follow the features of a river-type wetland and is not clear. In this park, the planting area of hygrophyte is far less than that of terrestrial plants. As a result, the wetland park is evolving into a public garden. It is suggested that the overall design of the park should be conducted in accordance with site characteristics and resources, and the park should be developed and built reasonably based on the principle that the ecology is a priority.

关键词： Urban wetland park Plants Investigation and analysis Huaxi

羚羊角及其角塞的傅里叶红外光谱快速识别研究

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光谱学与光谱分析 2010年第1期30卷 42-44页

作者：刘炎张贵君孙素琴北京中医药大学中药学院北京100102 清华大学化学系北京100084

采用傅里叶变换红外光谱(FTIR)技术,对羚羊角、羚羊角塞以及二者的混合物进行了对比研究。结果表明:羚羊角的红外光谱主要由蛋白质组分的特征吸收峰组成,而羚羊角塞的红外光谱主要由蛋白质和磷酸钙组分的特征吸收峰构成,二者的酰胺谱带... 详细信息

采用傅里叶变换红外光谱(FTIR)技术,对羚羊角、羚羊角塞以及二者的混合物进行了对比研究。结果表明:羚羊角的红外光谱主要由蛋白质组分的特征吸收峰组成,而羚羊角塞的红外光谱主要由蛋白质和磷酸钙组分的特征吸收峰构成,二者的酰胺谱带、C—O伸缩振动谱带以及CH基团的伸缩振动谱带也均有明显区别。通过上述差异,可有效地鉴别羚羊角、角塞以及掺有角塞的羚羊角样品。该研究为羚羊角的掺伪和杂质检查提供了快速、科学的新方法,也为羚羊角的成分与质量评价体系研究提供了一定的信息。

关键词：羚羊角角塞傅里叶变换红外光谱快速识别

Expression of estrogen receptor （ER） -α and -βtranscripts in the neonatal and adult rat cerebral cortex, cerebellum, and olfactory bulb

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Cell Research 2001年第4期11卷 321-324页

作者： GUO XING ZHONG, JUN DA su, QING WEN sun, BING HUA JIAO Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433 School of Life Sciences and Medical Engineering, Tongji University, Shanghai 200092, China Shangha Department of Biochemistry and Molecular Biology Second Military Medical University Shanghai School of Life Sciences and Medical Engineering Tongji University Shanghai China Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai China

In the present study expression of estrogen receptor subtype -α(ERα) and -β (ERβ) in the cerebral cortex, cerebellum, and olfactory bulb was investigated and compared between neonatal (1～3-days-old) and adult (250～350g) rats, using reverse transcription-polymerase chain reaction (RT- PCR). No ERα transcripts were detectable in the adult cerebellum and olfactory bulb, whereas very weak expression of ERα was present in the adult cerebral cortex. No significant difference in ERβ transcripts was detectable between the neonatal and adult rats. While transcripts for both ER subtypes were co-expressed in these brain areas of neonatal rats, although ERα expression was significantly weaker than ERβ. Even in the cerebral cortex known to contain both ER subtypes in adult rats, ERα transcripts in neonatal rats were much higher than in adult. These observations provide evidence for the existence of different expression patterns of ERcα/ERβ transcripts in these three brain areas between the neonatal and adult rats, suggesting that each ER subtype may play a distinct role in the regulation of differentiation, development, and functions of the brain by estrogen.

关键词： Cerebellum cerebral cortex estrogen receptor (ER) olfactory bulb RT-PCR.