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Overexpression of Dlx2 enhances osteogenic differentiation of BMSCs and MC3T3-E1 cells via direct upregulation of Osteocalcin and Alp

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International Journal of Oral Science 2019年第2期11卷 119-130页

作者： Jianfei Zhang Wenbin Zhang Jiewen Dai Xudong Wang steve Guofang Shen Department of Oral & Cranio-maxillofacial Surgery Shanghai Ninth People’s Hospital Shanghai Jiao Tong University School of Medicine Shanghai Key Laboratory of Stomatology

Genetic studies have revealed a critical role of Distal-homeobox (Dlx) genes in bone formation,and our previous study showed that Dlx2 overexpressing in neural crest cells leads to profound abnormalities of the craniofacial *** aim of this study was to investigate the role and the underlying molecular mechanisms of Dlx2 in osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) and pre-osteoblast MC3T3-E1 ***,we observed upregulation of Dlx2 during the early osteogenesis in BMSCs and MC3T3-E1 ***,Dlx2 overexpression enhanced alkaline phosphatase (ALP) activity and extracellular matrix mineralization in BMSCs and MC3T3-E1 cell *** addition,micro-CT of implanted tissues in nude mice confirmed that Dlx2 overexpression in BMSCs promoted bone formation in ***,Dlx2 overexpression had little impact on the expression level of the pivotal osteogenic transcription factors Runx2,Dlx5,Msx2,and Osterix,but led to upregulation of Alp and Osteocalcin (OCN),both of which play critical roles in promoting osteoblast ***,luciferase analysis showed that Dlx2 overexpression stimulated both OCN and Alp promoter *** chromatin-immunoprecipitation assay and site-directed mutagenesis analysis,we provide molecular evidence that Dlx2 transactivates OCN and Alp expression by directly binding to the Dlx2-response cis-acting elements in the promoter of the two *** on these findings,we demonstrate that Dlx2 overexpression enhances osteogenic differentiation in vitro and accelerates bone formation in vivo via direct upregulation of the OCN and Alp gene,suggesting that Dlx2 plays a crucial role in osteogenic differentiation and bone formation.

关键词： BMSCs MC3T3-E1 Osteocalcin

An all-optical polarization monitoring scheme for polarization division multiplexed transmission

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Chinese Optics Letters 2010年第10期8卷 979-982页

作者：叶佳闫连山易安林潘炜罗斌郭振 X.steve Yao Center for Information Photonics and Communications School of Information Science and Technology Southwest Jiaotong University General Photonics Corp.

A novel polarization monitoring scheme for polarization division multiplexed （PDM） systems is proposed. By using a tag light transmitted in different but close wavelength to the data signal as the feedback control, the scheme can demultiplex automatically two orthogonal polarizations in PDM systems. The effectiveness of the scheme is demonstrated experimentally in a 2×10-Gb/s on-off-keying （OOK） PDM transmission system.

关键词： Multiplexing

Mshpy23:a user-friendly,parameterized model of magnetosheath conditions

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Earth and Planetary Physics 2024年第1期8卷 89-104页

作者： Jaewoong Jung Hyunju Connor Andrew Dimmock steve Sembay Andrew Read Jan Soucek NASA Goddard Space Flight Center GreenbeltMDUSA Department of Astronomy University of MarylandCollege ParkMDUSA Swedish Institude of Space Physics UppsalaSweden University of Leicester LeicesterUK Institute of Atmospheric Physics Academy of Sciences of the Czech Republic

Lunar Environment heliospheric X-ray Imager(LEXI)and Solar wind−Magnetosphere−Ionosphere Link Explorer(SMILE)will observe magnetosheath and its boundary motion in soft X-rays for understanding magnetopause reconnection modes under various solar wind conditions after their respective launches in 2024 and *** conditions,namely,plasma density,velocity,and temperature,are key parameters for predicting and analyzing soft X-ray images from the LEXI and SMILE *** developed a userfriendly model of magnetosheath that parameterizes number density,velocity,temperature,and magnetic field by utilizing the global Magnetohydrodynamics(MHD)model as well as the pre-existing gas-dynamic and analytic *** this parameterized magnetosheath model,scientists can easily reconstruct expected soft X-ray images and utilize them for analysis of observed images of LEXI and SMILE without simulating the complicated global magnetosphere ***,we created an MHD-based magnetosheath model by running a total of 14 OpenGGCM global MHD simulations under 7 solar wind densities(1,5,10,15,20,25,and 30 cm)and 2 interplanetary magnetic field Bz components(±4 nT),and then parameterizing the results in new magnetosheath *** compared the magnetosheath model result with THEMIS statistical data and it showed good agreement with a weighted Pearson correlation coefficient greater than 0.77,especially for plasma density and plasma ***,we compiled a suite of magnetosheath models incorporating previous magnetosheath models(gas-dynamic,analytic),and did two case studies to test the *** MHD-based model was comparable to or better than the previous models while providing self-consistency among the magnetosheath ***,we constructed a tool to calculate a soft X-ray image from any given vantage point,which can support the planning and data analysis of the aforementioned LEXI and SMILE missions.A release of the code has been uploaded to a Git

关键词： magnetosheath python modeling

Investigation of Combustion of Emulated Biogas in a Gas Turbine Test Rig

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Journal of Thermal Science 2018年第4期27卷 331-340页

作者： Agustin VALERA-MEDINA Anthony GILES Daniel PUGH steve MORRIS Marcel POHL Andreas ORTWEIN Cardiff University Queen's Building Cardiff CF24 3AA United Kingdom DBFZ Deutsches Biomasseforschungszentrum gemeinnutzige GmbH Leipzig 04347 Germany Hochschule Merseburg Department Engineering and Natural Sciences Merseburg 06217 Germany

Combustion of biogas in gas turbines is an interesting option for provision of renewable combined heat and power from biomass. Due to an increasing share of fluctuating renewable energies in the power grid(especially from wind and solar power), flexible power generation is of increasing importance. Additionally, with an increasing share of agricultural and municipal waste in biogas production, biogas composition is expected to be within a broader range. In this paper, the combustion of synthetic biogas(carbon dioxide and methane) in a combustion test rig with a swirl burner and a high pressure optical chamber is researched at different conditions. Results are compared to a CHEMKIN-PRO simulation using a detailed reaction mechanism. The results show that within the researched experimental matrix, stable biogas combustion for gas turbines can be achieved even with significantly changing gas composition and nominal power. Carbon dioxide concentration is varied from 0 to 60%. CO concentrations(normalized to 15% O_2) in the flue gas do not change significantly with increasing carbon dioxide in the fuel gas and, for the researched conditions, stayed below 10 ppm. NO_x concentration is below 10 ppm(normalized to 15% O_2) for pure methane, and is further decreasing with increasing carbon dioxide share in the fuel gas, which is mainly due to changing reaction paths as reaction analysis showed. Thermal load of the combustor is varied from 100% to 20% for the reference gas composition. With decreasing thermal load, normalized carbon monoxide flue gas concentration is further reduced, while NOx concentrations are remaining at a similar level around 5 ppm(normalized to 15% O_2).

关键词： gas turbine biogas combustion test chemiluminescence

How Can Practitioners Support Citizen Volunteers in Disaster Risk Reduction?Insight from“Good and Ready”in Aotearoa New Zealand

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International Journal of Disaster Risk Science 2024年第3期15卷 374-387页

作者： Loïc Le Dé steve Ronoh Ei Mon Thinn Kyu Brigitte Rive School of Public Health and Interdisciplinary Studies Auckland University of TechnologyAuckland2025New Zealand Pacific Health Research Centre Auckland University of TechnologyAuckland2025New Zealand New Zealand Red Cross Auckland2241New Zealand

Global and national policy frameworks emphasize the importance of people's participation and volunteers'role in disaster risk *** research has extensively focused on volunteers in disaster response and recovery,less attention has been paid on how organizations involved in disaster risk management can support volunteers in leading and coordinating communitybased disaster risk *** 2019,the New Zealand Red Cross piloted the Good and Ready initiative in Auckland,Aotearoa New Zealand,with the objective to empower local people in resilience building with a focus on volunteers and community *** research examined the positive and negative outcomes of Good and Ready and investigated volunteers'experiences in the disaster resilience *** involved the codesign of a questionnaire-based survey using participatory methods with Good and Ready volunteers,the dissemination of the survey to gather volunteers'viewpoints,and a focus group discussion with participatory activities with Red Cross *** findings highlight that a key challenge lies in finding a balance between a program that provides flexibility to address contextual issues and fosters communities'ownership,versus a prescriptive and standardized approach that leaves little room for creativity and *** pinpoints that supporting volunteers with technical training is critical but that soft skills training such as coordinating,communicating,or facilitating activities at the local level are *** concludes that the sustainability of Good and Ready requires understanding and meeting volunteers'motivations and expectations and that enhancing partnerships with local emergency management agencies would strengthen the program.

关键词： Community-based approach Disaster risk reduction New Zealand Participation Volunteers

The Subtelomere of Oryza sativa Chromosome 3 Short Arm as a Hot Bed of New Gene Origination in Rice

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Molecular Plant 2008年第5期1卷 839-850页

作者： Chuanzhu Fana Yong Zhang Yeisoo Yu steve Rounsley Manyuan Long Rod A. Wing Arizona Genomics Institute Department of Plant Sciences University of Arizona Tucson AZ 85721 USA Department of Ecology and Evolution University of Chicago Chicago IL 60637 USA BIO5 Institute for Collaborative Research University of Arizona Tucson AZ 85721 USA

Despite general observations of non-random genomic distribution of new genes, it is unclear whether or not new genes preferentially occur in certain genomic regions driven by related molecular mechanisms. Using 1.5 Mb of genomic sequences from short arms of chromosome 3 of Oryza glaberrima and O. punctata, we conducted a comparative genomic analysis with the reference O. sativa ssp. japonica genome. We identified a 60-kb segment located in the middle of the subtelomeric region of chromosome 3, which is unique to the species O. sativa. The region contained gene duplicates that occurred in Asian cultivated rice species that diverged from the ancestor of Asian and African cultivated rice one million years ago （MYA）. For the 12 genes and one complete retrotransposon identified in this segment in O. sativa ssp. japonica, we searched for their parental genes. The high similarity between duplicated paralogs further supports the recent origination of these genes. We found that this segment was recently generated through multiple independent gene recombination and transposon insertion events. Among the 12 genes, we found that five had chimeric gene structures derived from multiple parental genes. Nine out of the 12 new genes seem to be functional, as suggested by Ka/Ks analysis and the presence of cDNA and/or MPSS data. Furthermore, for the eight transcribed genes, at least two genes could be classified as defense or stress response-related genes. Given these findings, and the fact that subtelomeres are associated with high rates of recombination and transcription, it is likely that subtelomeres may facilitate gene recombination and transposon insertions and serve as hot spots for new gene origination in rice genomes.

关键词： comparative genomics gene duplication Oryza sativa subtelomere new genes.

Research on the Challenges Facing Curriculum Developers in Transitioning from Offline to Online Teacher Training:A Case Study of the National Institute for Educational Development in Namibia

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Research on the Challenges Facing Curriculum Developers in T...

作者： LILUNGWE steve NYAMBE 浙江师范大学

学位级别：硕士

The National Institute for Educational Development(NIED)is a directorate within the Ministry of Basic Education,Arts and Culture(Mo EAC)in the republic of *** was established by act of parliament in 1990 to be responsible for the development and spearheading of basic education reform or the national curriculum for schools from pre-primary to grade 12/ advanced ***'s also responsible for the continuous professional development of *** to the poor economic status of the country and other emergencies such as pandemics,the ministry has put directives or changed the policy to discourage travelling allowances for staff members to conduct face-to-face workshops and seminars due to budgetary constraints;the National Institute for Educational Development(NIED)adopted the use of online mode to conduct continuous professional development of *** objective of this study was to describe internal,external and individual challenges faced by curriculum developers in terms of education provision to transition from offline to online teacher *** study was guided by three fundamental research questions,namely;what are the challenges facing curriculum developers in terms of education provision in transitioning from offline to online teacher training,what are the challenges facing curriculum developers in conducting teacher training using online medium and what are the suggestions from different departments and Institutions on how to solve the challenges faced by curriculum developers in using online medium to train teachers? A qualitative and quantitative research method was used in this study to extract quality findings from 43 participants which were selected from the two divisions of curriculum development and professional *** research identified key challenges as lack of an adequate supply of ICT resources,lack of quality internet service providers,lack of training on the usage of the Clevertouch smart screen,lack of knowledge

关键词： Curriculum Developers Transition Online Teacher Training

De novo serine synthesis regulates chondrocyte proliferation during bone development and repair

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Bone Research 2022年第2期10卷 274-285页

作者： steve Stegen Shauni Loopmans Ingrid Stockmans Karen Moermans Peter Carmeliet Geert Carmeliet Laboratory of Clinical and Experimental Endocrinology Department of Chronic Diseases and MetabolismKU Leuven3000 LeuvenBelgium Laboratory of Angiogenesis and Vascular Metabolism VIB Center for Cancer Biology3000 LeuvenBelgium Laboratory of Angiogenesis and Vascular Metabolism Department of Oncology and Leuven Cancer InstituteKU Leuven3000 LeuvenBelgium State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-Sen UniversityGuangzhou 510080China

The majority of the mammalian skeleton is formed through endochondral ossification starting from a cartilaginous *** cells, or chondrocytes, survive, proliferate and synthesize extracellular matrix in an avascular environment, but the metabolic requirements for these anabolic processes are not fully understood. Here, using metabolomics analysis and genetic in vivo models, we show that maintaining intracellular serine homeostasis is essential for chondrocyte function. De novo serine synthesis through phosphoglycerate dehydrogenase(PHGDH)-mediated glucose metabolism generates nucleotides that are necessary for chondrocyte proliferation and long bone growth. On the other hand, dietary serine is less crucial during endochondral bone formation, as serine-starved chondrocytes compensate by inducing PHGDH-mediated serine ***, this metabolic flexibility requires ATF4, a transcriptional regulator of amino acid metabolism and stress *** demonstrate that both serine deprivation and PHGDH inactivation enhance ATF4 signaling to stimulate de novo serine synthesis and serine uptake, respectively, and thereby prevent intracellular serine depletion and chondrocyte dysfunction. A similar metabolic adaptability between serine uptake and de novo synthesis is observed in the cartilage callus during fracture ***, the results of this study reveal a critical role for PHGDH-dependent serine synthesis in maintaining intracellular serine levels under physiological and serine-limited conditions, as adequate serine levels are necessary to support chondrocyte proliferation during endochondral ossification.

关键词： synthesis starting thereby

Wastewater Based Surveillance of SARS-CoV-2:Challenges and Perspective from a Canadian Inter-laboratory Study

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Journal of Environmental Sciences 2022年第6期34卷 229-232页

作者： Teresa Kumblathan Nicholas Piroddi steve E.Hrudey Xing-Fang Li Division of Analytical and Environmental Toxicology Department of Laboratory Medicine and PathologyFaculty of Medicine and DentistryUniversity of AlbertaAlbert T6G 2G3aCanada

The current rapidly rising 5^(th) wave of the Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2),dominated by the omicron variant,has clearly shown the challenges in monitoring,and tracing clinical cases of *** has been identified by the World Health Organization(WHO)as a variant of concern(VOC)on November 26^(th) of 2021,because of the reported higher risk of transmission,reinfection.

关键词： Wastewater infection. Canadian