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A new osteogenic protein isolated from Dioscorea opposita Thunb accelerates bone defect healing through the mTOR signaling axis

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Bioactive Materials 2023年第9期27卷 429-446页

作者： John Akrofi Kubi Augustine Suurinobah Brah Kenneth Man Chee Cheung Yin Lau Lee Kai-Fai Lee stephen cho wing sze Wei Qiao Kelvin Wai-Kwok Yeung Department of Orthopaedics and Traumatology Li Ka Shing Faculty of MedicineThe University of Hong Kong(HKU)Hong Kong S.A.R.PR China Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma HKU-Shenzhen HospitalShenzhen518053PR China Department of Obstetrics and Gynaecology Li Ka Shing Faculty of MedicineHKU21 Sassoon RoadHong Kong S.A.RPR China Shenzhen Key Laboratory of Fertility Regulation Reproductive Medicine CenterHKU-Shenzhen HospitalShenzhenPR China Department of Biology Faculty of ScienceHong Kong Baptist UniversityKowloon TongHong Kong S.A.RPR China Golden Meditech Center for NeuroRegeneration Sciences Hong Kong Baptist UniversityKowloon TongHong Kong S.A.RPR China Applied Oral Sciences and Community Dental Care Faculty of DentistryHong Kong S.A.RPR China

Delayed bone defect repairs lead to severe health and socioeconomic impacts on patients. Hence, there are increasing demands for medical interventions to promote bone defect healing. Recombinant proteins such as BMP-2 have been recognized as one of the powerful osteogenic substances that promote mesenchymal stem cells (MSCs) to osteoblast differentiation and are widely applied clinically for bone defect repairs. However, recent reports show that BMP-2 treatment has been associated with clinical adverse side effects such as ectopic bone formation, osteolysis and stimulation of inflammation. Here, we have identified one new osteogenic protein, named ‘HKUOT-S2’ protein, from Dioscorea opposita Thunb. Using the bone defect model, we have shown that the HKUOT-S2 protein can accelerate bone defect repair by activating the mTOR signaling axis of MSCs-derived osteoblasts and increasing osteoblastic biomineralization. The HKUOT-S2 protein can also modulate the transcriptomic changes of macrophages, stem cells, and osteoblasts, thereby enhancing the crosstalk between the polarized macrophages and MSCs-osteoblast differentiation to facilitate osteogenesis. Furthermore, this protein had no toxic effects in vivo. We have also identified HKUOT-S2 peptide sequence TKSSLPGQTK as a functional osteogenic unit that can promote osteoblast differentiation in vitro. The HKUOT-S2 protein with robust osteogenic activity could be a potential alternative osteoanabolic agent for promoting osteogenesis and bone defect repairs. We believe that the HKUOT-S2 protein may potentially be applied clinically as a new class of osteogenic agent for bone defect healing.

关键词： Dioscorea spp protein Mesenchymal stem cells(MSCs) Osteoblast differentiation Bone mineral density(BMD) Bone defect repair mTOR signaling pathway

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Combing Network Phytochemistry with Networking Pharmacology Approaches to Elucidate the Action Mechanism of Traditional Chinese Medicine Formula:Chemical Constitutes of Erxian Decoction Up-Regulated Estrogen Receptor Expression and Induced Estradiol-Biosyn

Combing Network Phytochemistry with Networking Pharmacology ...

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第十四届全国中药和天然药物学术研讨会

作者： Shiwei Wang Tzi-Bun Ng Lixing Lao stephen cho wing sze The University of Hong Kong The Chinese University of Hong Kong

＜正＞Background Menopause is an aging process due to estrogen deficiency caused by ovarian *** include osteoporosis *** replacement therapy used for relieving menopausal symptoms can increase the incidence of breast ...

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Science-based Holistic Philosophy of Chinese Medicine Formula:Reflected in cytotoxicity of Tian-xian Liquid（in vitro） and the protein expression of p21,MMP-1 and MDR-1 in Human Colorectal Carcinoma（in vivo） in Response to Tian-xian Liquid

Science-based Holistic Philosophy of Chinese Medicine Formul...

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第十四届全国中药和天然药物学术研讨会

作者： Annballaw Bridget Leigh Yau Ming Lai Tzi Bun.Ng Yao Tong stephen cho wing sze The University of Hong Kong The Hong Kong Polytechnic University The Chinese University of Hong Kong

＜正＞Aim This study aims at investigating the holistic effects of three functional constituent fractions obtained from Tian-xian Liquid（TXL）contributing to anti-tumorigenicity of TXL in human colorectal carcinoma i...

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Comparative Analysis of Estradiol-stimulating Proteins from Four Different Dioscorea Species

Comparative Analysis of Estradiol-stimulating Proteins from ...

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第十四届全国中药和天然药物学术研讨会

作者： Jia Lu Ricky NS Wong Liang Zhang Lixing Lao Yanbo Zhang Tzi Bun Ng stephen cho wing sze The University of Hong Kong Hong Kong Baptist University The Chinese University of Hong Kong

＜正＞Background Dioscorea plants are used in traditional Chinese medicine as a single herbal medicine or formula for relieving menopausal *** previously isolated an estradiol-stimulating protein,DOI,from the rhizomes of Dioscorea opposite,one of classical Chinese herbal medicines clinically used for treating menopausal ***-like proteins have herein

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Home range establishment and utilization by reintroduced lions(Panthera leo)in a small South African wildlife reserve

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Integrative Zoology 2017年第4期12卷 318-332页

作者： sze-wing YIU Francesca PARRINI Leszek KARCZMARSKI Mark KEITH The Swire Institute of Marine Science School of Biological Sciencesthe University of Hong KongHong Kong SARChina Centre for African Ecology School of AnimalPlant and Environmental SciencesUniversity of the WitwatersrandJohannesburgSouth Africa Centre for Wildlife Management Department of Animal and Wildlife SciencesUniversity of PretoriaPretoriaSouth Africa

Understanding of animal spatial behavior is essential for informed management *** southern Africa,reintroduction of lions(Panthera leo)to small reserves(60 separate visits)around the largest dam and along rivers sugge... 详细信息

Understanding of animal spatial behavior is essential for informed management *** southern Africa,reintroduction of lions(Panthera leo)to small reserves(wing 3 years their home range sizes continued to increase,but in each individual case the size remained smaller than half of the reserve area(60 separate visits)around the largest dam and along rivers suggest the importance of water and its surrounding vegetation in the lions’space utilization *** home range size did not differ with season or sex of the individuals,whereas shifts in locations of home ranges revealed differences in the response of the 2 sexes to territorial conflicts and management *** study shows a dynamic home range utilization pattern and highlights the importance of both fine-scale space use patterns(frequency and duration of visits)and broadscale home range changes in understanding the ranging behavior of reintroduced animals.

关键词： carnivore movement space use T-LoCoH utilization distribution

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Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β

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Chinese Journal of Integrative Medicine 2022年第3期28卷 229-235页

作者： LIN Kai-li ZHANG Ji CHUNG Hau-lam WU Xin-yi LIU Bin ZHAO Bo-xin sze stephen cho-wing ZHOU Ping-zheng YUNG Ken Kin-lam ZHANG Shi-qing School of Public Health Guangzhou Medical UniversityGuangzhou(511436)China Department of Biology Hong Kong Baptist University(HKBU)Hong Kong Special Administrative Region(999077)China HKBU Shenzhen Research Institute and Continuing Education ShenzhenGuangdong Provinee(518057)China Department of Plastic Surgery Zhujiang HospitalSouthern Medical UniversityGuangzhou(510515)China Guangzhou Institute of Cardiovascular Disease the Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou(510260)China Department of Pharmacy Nan fang HospitalSouther n Medical Un iversityGuan gzhou(510515)China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou(510515)China

Objective:To study the effects of total ginsenosides(TG)extract from Panax ginseng on neural stem cell(NSC)proliferation and differentiation and their underlying ***:The migration of NSCs after treatment with various concentrations of TG extract(50,100,or 200μg/mL)were *** proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere *** differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2(MAP2).The GSK-3β/β-catenin pathway in TG-treated NSCs was examined by Western blot *** NSCs with constitutively active GSK-3βmutant were made by adenovirus-mediated gene transfection,then the proliferation and differentiation of NSCs mediated by TG were further ***:TG treatment significantly enhanced NSC migration(P<0.01 or P<0.05)and increased the proliferation of NSCs(P<0.01 or P<0.05).TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression(P<0.01 or P<0.05).TG extract also significantly induced GSK-3βphosphorylation at Ser9,leading to GSK-3βinactivation and,consequently,the activation of the GSK-3β/β-catenin pathway(P<0.01 or P<0.05).In addition,constitutive activation of GSK-3βin NSCs by the transfection of GSK-3βS9 A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation(P<0.01 or P<0.05).Conclusion:TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3β.

关键词： ginsenoside neural stem cell proliferation neuronal differentiation GSK-3β

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Monitoring interannual dynamics of desertification in Minqin County,China,using dense Landsat time series

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International Journal of Digital Earth 2020年第8期13卷 886-898页

作者： Xiaolin Zhu Kwok Huen Leung wing sze Li Lek Kin Cheung The Department of Land Surveying and Geo-Informatics The Hong Kong Polytechnic UniversityHong KongPeople’s Republic of China The Hong Kong Polytechnic University Shenzhen Research Institute ShenzhenPeople’s Republic of China

Minqin County in northwestern China is highly affected by *** have been initiated in recent decades to combat desertification in *** assess the effectiveness of these campaigns,this study used a dense Landsat time series from 1987 to 2017 to investigate the interannual dynamics of vegetation coverage and greenness over the past 31 ***,this study applied an advanced technology to reconstruct a high-quality Landsat annual time ***,one image in the vegetation-peak season was selected as the base image in each year,and then problematic pixels were interpolated by the neighborhood similar pixel interpolator using ancillary images in the same ***,the land cover map and the enhanced vegetation index(EVI)were derived from all reconstructed ***,the change of vegetation coverage and EVI values over the 31 years were *** results show that the total vegetation coverage and greenness increased during the 31 *** this change trend to other factors suggests that vegetation in Minqin County is highly affected by agriculture and groundwater supply rather than by *** mitigate desertification in a sustainable way,agriculture should be well managed to avoid overconsumption of natural resources such as underground water.

关键词： Minqin County desertification agriculture Landsat time series vegetation cover

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Exact Solution for Thermal Stagnation-Point Flow with Surface Curvature and External Vorticity Effects

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Journal of Applied Mathematics and Physics 2017年第4期5卷 966-989页

作者： Ronald Ming cho So Elizabeth wing sze Kam Mechanical Engineering Department the Hong Kong Polytechnic University Hong Kong China Department of Mathematical Sciences Rensselaer Polytechnic Institute Troy NY USA

Exact solution of the steady Navier-Stokes equations has been obtained for the thermal stagnation-point flow at the leading edge of a turbine blade under the assumptions of constant nose radius and external vorticity, and fluid properties independent of temperature. The solutions reveal that curvature affects local heat transfer and skin friction while external vorticity does not. The effect of external vorticity is to shift the zero skin friction point away from the stagnation point. This solution is valid for all Reynolds number, external vorticity, and nose radius. In the limit of nose radius going to infinity and external vorticity, going to zero, the exact solution for two-dimensional plane stagnation-point flow is recovered identically. In addition, it can be shown that the velocity field around the stagnation point of a rotating curved surface is the same as that around the stagnation point of a stationary curved surface with an external vorticity which equals to twice of the rotational speed. This realization renders the present solution equally valid for thermal stagnation point flow at the leading edge of centrifugal impeller blades.

关键词： Navier-Stokes Equations Exact Solutions Thermal Stagnation-Point Flow Displacement Effect

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Applications and potentials of nanopore sequencing in the(epi)genome and(epi)transcriptome era

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The Innovation 2021年第4期2卷 11-26页

作者： Shangqian Xie Amy wing-sze Leung Zhenxian Zheng Dake Zhang Chuanle Xiao Ruibang Luo Ming Luo Shoudong Zhang Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants College of ForestryHainan UniversityHaikou 570228China Department of Computer Science The University of Hong KongHong Kong 999077China Beijing Advanced Innovation Centre for Biomedical Engineering Key Laboratory for Biomechanics and Mechanobiology of Ministry of EducationSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing 100083China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CentreSun Yat-sen UniversityGuangzhou 510060China Agriculture and Biotechnology Research Center Guangdong Provincial Key Laboratory of Applied BotanyCenter of Economic BotanyCore Botanical GardensSouth China Botanical GardenChinese Academy of SciencesGuangzhou 510650China School of Life Sciences The Chinese University of Hong KongShatinHong Kong 999077China Center for Soybean Research of the State Key Laboratory of Agrobiotechnology The Chinese University of Hong KongShatinHong Kong 999077China

The Human Genome Project opened an era of(epi)genomic research,and also provided a platform for the development of new sequencing *** and after the project,several sequencing technologies continue to dominate nucleic acid sequencing ***,Illumina(short-read),PacBio(long-read),and Oxford Nanopore(longread)are the most popular sequencing *** PacBio or the popular short-read sequencers before it,which,as examples of the second or so-called Next-Generation Sequencing platforms,need to synthesize when sequencing,nanopore technology directly sequences native DNA and RNA *** sequencing,therefore,avoids converting mRNA into cDNA molecules,which not only allows for the sequencing of extremely long native DNA and full-length RNA molecules but also document modifications that have been made to those native DNA or RNA *** this review on direct DNA sequencing and direct RNA sequencing using Oxford Nanopore technology,we focus on their development and application achievements,discussing their challenges and future *** also address the problems researchers may encounter applying these approaches in their research topics,and how to resolve them.

关键词： nanopore sequencing direct DNA sequencing direct RNA sequencing base modification base-calling long-read sequencing tools and algorithms

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Isolated penile urethral injury:A rare case following male coital trauma

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Asian Journal of Urology 2015年第3期2卷 175-178页

作者： Ka wing Wong Brian sze Ho Ho Chi Ho Ip Ming Kwong Yiu Division of Urology Department of SurgeryUniversity of Hong KongPokfulamHong KongChina

Penile fractures are an uncommon urological ***,penile fractures involve the corpus cavernosum and are sometimes associated with urethral *** corpus spongiosum and urethral injuries without concomitant corpus cavernosum injury are,however,*** proper knowledge of the management of penile fractures and urethral injuries,this distinct entity can be diagnosed,assessed and managed successfully without complications.

关键词： Penile fracture Urethral injury Penis Corpus spongiosum Urological trauma Urethroplasty

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