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On-chip multiplexed single-cell patterning and controllable intracellular delivery

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Microsystems & Nanoengineering 2020年第1期6卷 1222-1232页

作者： Zaizai Dong Yanli Jiao Bingteng Xie Yongcun Hao Pan Wang Yuanyuan Liu Junfeng Shi Chandani Chitrakar stephen black Yu-Chieh Wang LJames Lee Mo Li Yubo Fan Lingqian Chang School of Biological Science and Medical Engineering Beihang University100083 BeijingChina Institute of Nanotechnology for Single Cell Analysis(INSCA) Beijing Advanced Innovation Center for Biomedical EngineeringBeihang University100083 BeijingChina College of Agricultural and Life Sciences University of FloridaGainesvilleFL 32611USA Center for Reproductive Medicine Peking University Third Hospital100191 BeijingChina Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace Northwestern Polytechnical University710072 Xi’anChina Chemical and Biomolecular Engineering Department Ohio State UniversityColumbusOH 43209USA Department of Biomedical Engineering University of North TexasDentonTX 76207USA Department of Pharmaceutical Sciences University of North Texas Health Science CenterFort WorthTX 76107USA

Conventional electroporation approaches show limitations in the delivery of macromolecules in vitro and in *** limitations include low efficiency,noticeable cell damage and nonuniform delivery of ***,we present a simple 3D electroporation platform that enables massively parallel single-cell manipulation and the intracellular delivery of macromolecules and small molecules.A pyramid pit micropore array chip was fabricated based on a silicon wet-etching method.A controllable vacuum system was adopted to trap a single cell on each *** this chip,safe single-cell electroporation was performed at low *** of various sizes ranging from oligonucleotides(molecular beacons,22 bp)to plasmid DNA(CRISPR-Cas9 expression vectors,>9 kb)were delivered into targeted cells with a significantly higher transfection efficiency than that of multiple benchmark methods(e.g.,commercial electroporation devices and Lipofectamine).The delivered dose of the chemotherapeutic drug could be controlled by adjusting the applied *** using CRISPR-Cas9 transfection with this system,the p62 gene and CXCR7 gene were knocked out in tumor cells,which effectively inhibited their cellular ***,this vacuum-assisted micropore array platform provides a simple,efficient,high-throughput intracellular delivery method that may facilitate on-chip cell manipulation,intracellular investigation and cancer therapy.

关键词： inhibited vacuum limitations

SARS-CoV-2 spike protein induces IL-18-mediated cardiopulmonary inflammation via reduced mitophagy

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1901-1915页

作者： Shuxin Liang Changlei Bao Zi Yang Shiyun Liu Yanan Sun Weitao Cao Ting Wang Tae-Hwi Schwantes-An John S.Choy Samisubbu Naidu Ang Luo Wenguang Yin stephen M.black Jian Wang Pixin Ran Ankit A.Desai Haiyang Tang State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangdong Key Laboratory of Vascular DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina College of Veterinary Medicine Northwest A&F UniversityYanglingShaanxiChina Department of Cellular Biology&Pharmacology Herbert Wertheim College of MedicineMiamiFLUSA Department of Environmental Health Sciences Robert Stempel College of Public Health and Social Work and Center for Translational ScienceFlorida International UniversityPort St.LucieFLUSA Department of Medical and Molecular Genetics Indiana University School of MedicineIndianapolisINUSA Department of Biology The Catholic University of AmericaWashingtonDCUSA Krannert Institute of Cardiology Department of MedicineIndiana UniversityIndianapolisINUSA Guangzhou Laboratory GuangzhouChina

Cardiopulmonary complications are major drivers of mortality caused by the SARS-CoV-2 ***-18,an inflammasomeinduced cytokine,has emerged as a novel mediator of cardiopulmonary pathologies but its regulation via SARS-CoV-2 signaling remains *** on a screening panel,IL-18 was identified amongst 19 cytokines to stratify mortality and hospitalization burden in patients hospitalized with *** clinical data,administration of SARS-CoV-2 Spike 1(S1)glycoprotein or receptor-binding domain(RBD)proteins into human angiotensin-converting enzyme 2(hACE2)transgenic mice induced cardiac fibrosis and dysfunction associated with higher NF-κB phosphorylation(pNF-κB)and cardiopulmonary-derived IL-18 and NLRP3 ***-18 inhibition via IL-18BP resulted in decreased cardiac pNF-κB and improved cardiac fibrosis and dysfunction in S1-or RBD-exposed hACE2 *** in vivo and in vitro work,both S1 and RBD proteins induced NLRP3 inflammasome and IL-18 expression by inhibiting mitophagy and increasing mitochondrial reactive oxygenation *** mitophagy prevented Spike protein-mediated IL-18 ***,IL-18 inhibition reduced Spike protein-mediated pNF-κB and EC ***,the link between reduced mitophagy and inflammasome activation represents a novel mechanism during COVID-19 pathogenesis and suggests IL-18 and mitophagy as potential therapeutic targets.

关键词： NLRP3 expression cardiopulmonary

瞬感血糖监测设备瞬感血糖监测设备:CCG的标准不一致

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英国医学杂志中文版 2019年第11期22卷 685-685页

作者： stephen black 余洁(译) 许岭翎(校) black Box Data Science Biggleswade SGI8 OQAUK 北京协和医院内分泌科

由医疗委员小组(CCGs)编写的不一致的获取瞬感血糖监测设备的标准,比产生邮政编码的彩票更令人担忧,因为它们会严重损害我们在未来做出更好决策的能力1.几乎没有标准有任何意义。它们看起来像一组经过经济决策后编造出来的临床借口,以... 详细信息

由医疗委员小组(CCGs)编写的不一致的获取瞬感血糖监测设备的标准,比产生邮政编码的彩票更令人担忧,因为它们会严重损害我们在未来做出更好决策的能力1.几乎没有标准有任何意义。它们看起来像一组经过经济决策后编造出来的临床借口,以便使瞬感血糖监测设备被处方得越少越好。

关键词：血糖监测 CCG 严重损害邮政编码决策标准

Transport institutions and organisations in the formulation of policies for Australian local area traffic management:A 50-year retrospective

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Journal of Traffic and Transportation Engineering(English Edition) 2023年第5期10卷 866-877页

作者： John black School of Civil and Environmental Engineering University of New South WalesSydneyNSW 2052Australia

A review of 115 studies on Australian local area traffic management(LATM)schemes covers network planning,computer modelling,overall design considerations,the deployment of various traffic control devices,project evaluation and numerous before and after case ***,no research has been published about the formulation of LATM policies and the processes involved that were formulated during the 1970s and 1980s and aimed at discouraging non-local through traffic in residential areas,improving road safety,and improving environmental amenity through physical *** paper develops a conceptual model of the interactions amongst institutions of government(state and local),organisations(national research institutes and universities),and civil society(the consulting industry,lobby groups and community action groups).The model is implemented through a series of unstructured interviews with key players involved with research and advocacy,capacity building,and state government policy makers that determined:who was responsible for the governance of LATM schemes?What were the respective roles of institutions and organisations in relation to the early formulation of policies and plans,especially issues of authority?Who were the key players in these institutions and organisations?To what extent did external influences of ideas by overseas agents(policy transfer)occur in decision making?A recently implemented LATM scheme(Seven Ways)by Waverley Council describes the latest approaches,including community *** conclusions note the importance of a society investing in road research,having universities capable of delivering high-quality professional development programs,and having a consulting industry that is willing to deliver innovative,practical advice to local *** are made about areas for further research.

关键词： Transport policy Policy formulation Conceptual model LATM Transport institutions and organisations

Global Change in Agricultural Flash Drought over the 21st Century

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Advances in Atmospheric Sciences 2024年第2期41卷 209-220,I0002-I0019页

作者： Emily black National Centre for Atmospheric Science Fairbairn House71-75 Clarendon RoadLeedsLS29PHU.K Department of Meteorology Brian Hoskins BuildingUniversity of ReadingWhiteknights campusReadingRG66ETU.K.

Agricultural flash droughts are high-impact phenomena, characterized by rapid soil moisture dry down. The ensuing dry conditions can persist for weeks to months, with detrimental effects on natural ecosystems and crop cultivation. Increases in the frequency of these rare events in a future warmer climate would have significant societal impact. This study uses an ensemble of 10 Coupled Model Intercomparison Project(CMIP) models to investigate the projected change in agricultural flash drought during the 21st century. Comparison across geographical regions and climatic zones indicates that individual events are preceded by anomalously low relative humidity and precipitation, with long-term trends governed by changes in temperature, relative humidity, and soil moisture. As a result of these processes, the frequency of both upperlevel and root-zone flash drought is projected to more than double in the mid-and high latitudes over the 21st century, with hot spots developing in the temperate regions of Europe, and humid regions of South America, Europe, and southern Africa.

关键词： flash drought climate change soil moisture agricultural drought CMIP

Review of current method to determine outburst threshold limits in Australian underground coal mines

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International Journal of Mining Science and Technology 2019年第6期29卷 859-865页

作者： Dennis J.black CoalGAS Department of Civil Mining and Environmental Engineering University of Wollongong

Desorption rate index(DRI)was presented to the Australian underground coal industry in 1995 as a means for determining outburst threshold limits for Australian coal *** is a measure of the gas volume released from a coal sample in the first 30 s of crushing during the Q3 stage of gas content testing,multiplied by the ratio between measured Q3 and *** were identified between QM and DRI for both CO2 and CH4 rich coal samples collected from the Bulli Seam at West Cliff Colliery and that identified relationship was referred to as the Bulli Seam *** outburst mining gas content threshold limit values specified for the Bulli Seam at that time,when applied to the QM-DRI Bulli Seam benchmark,was shown to closely align with a DRI value of 900(DRI900),for both CO2 and CH4 rich seam gas *** Australian coal industry adopted the DRI900 as the basis for determining outburst gas content TLV for Australian coal *** mining experience in Australia has shown that gas content is not the only significant factor that impacts outburst risk,as all significant outburst events have been associated with abnormal geological conditions,such as faults and ***,assessing the potential application of additional outburst risk factors,to accurately define outburst risk zones,set safe mining threshold levels,and determine appropriate mining controls,warrants further *** Australian coal mines have implemented mining procedures enabling mining to continue in areas with gas content greater than the TLV determined using the DRI900 approach,without inducing an *** is a broad lack of understanding among Australian coal mine operators as to the procedure and calculations used to determine ***,there has been growing concern regarding the accuracy and validity of the DRI900 method for determining outburst TLV.A comprehensive set of gas data has been collected from Australian coal seams,including the Bulli Seam,and t

关键词： Outburst Desorption rate DRI Threshold limit

Review of coal and gas outburst in Australian underground coal mines

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International Journal of Mining Science and Technology 2019年第6期29卷 815-824页

作者： Dennis J.black CoalGAS Department of Civil Mining and Environmental Engineering University of Wollongong

Outburst of coal and gas represents a significant risk to the health and safety of mine personnel working in development and longwall production face *** have been over 878 outburst events recorded in twenty-two Australian underground coal *** outburst incidents have been associated with abnormal geological *** of Australian outburst incidents and mining experience in conditions where gas content was above current threshold levels are presented and *** experience suggests that for gas con-tent below 9.0 m^3/t,mining in carbon dioxide(CO2)rich seam gas conditions does not pose a greater risk of outburst than mining in CH4 rich seam gas *** experience also suggests that where no abnormal geological structures are present that mining in areas with gas content greater than the current accepted threshold levels can be undertaken with no discernible increase in outburst *** current approach to determining gas content threshold limits in Australian mines has been effective in prevent-ing injury from outburst,however operational experience suggests the current method is overly conser-vative and in some cases the threshold limits are low to the point that they provide no significant reduction in outburst *** factors that affect outburst risk,such as gas pressure,coal toughness and stress and geological structures are presently not incorporated into outburst threshold limits adopted in Australian *** factors and the development of an outburst risk index applicable to Australian underground coal mining conditions are the subject of ongoing research.

关键词： Outburst Desorption rate DRI Threshold limit

Investigations into the identification and control of outburst risk in Australian underground coal mines

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International Journal of Mining Science and Technology 2017年第5期27卷 749-753页

作者： Dennis J.black PacificMGM Bulli 2516Australia Department of Civil Mining and Environmental EngineeringUniversity of WollongongWollongong 2500Australia

Australian coal mines currently use gas content to assess outburst risk. The gas content threshold values for each mine are indirectly determined from measurement of gas volume liberated from 150 g coal samples during Q_3 residual gas content testing. It has been more than twenty years since this method, known as desorption rate index(DRI), was presented to the Australian coal industry, and in that time, there have been significant changes in mining conditions and the outburst threshold limits used at the benchmark Bulli seam mines. NSW Regulations list matters to be considered in developing control measures to manage the risk of gas outburst, and specifies that gas content, or DRI method, is used as the basis for determining outburst control zone. Whilst Queensland Regulations state that a coal or rock outburst is a high potential incident, there is no guidance provided to assist mine operators to define outburst prone conditions. A research project is planned at UOW to investigate the application of the DRI method and other potentially significant factors, such as gas pressure, coal toughness and permeability, which can be utilised by mine operators to assess outburst risk and determine appropriate outburst threshold limits and controls.

关键词： Outburst Desorption rate DRI Threshold limit

Clustering spatio-temporal bi-partite graphs for finding crowdsourcing communities in IoMT networks

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Big Earth Data 2021年第1期5卷 24-48页

作者： Kaine black Monica Wachowicz Department of Geodesy and Geomatics Engineering University of New BrunswickFrederictonCanada School of Geospatial Science RMIT UniversityMelbourneAustralia

The Internet of Moving Things is rapidly becoming a reality where intelligent devices and infrastructures are fostering real-time data sus-tainability in smart cities and advancing crowdsourced tasks to improve energy consumption,waste management,and traffic *** intelligent devices create a complex network scenario in which they often move together or in conjunction with one another to complete crowdsourced *** research premise is that mobility relationships matter when performing these tasks,and therefore,a graph model based on representing the changes in mobility relation-ships is needed to help identify the neighbour devices that are moving close to one another in our physical world but also seamlessly con-nected in their virtual *** propose a bi-partite community mobility graph model for linking intelligent devices in both virtual and physical worlds,as well as reaching a trade-off between crowd-sourced tasks designed with explicit and implicit citizen *** paper aims to explore a bi-partite graph as a promising spatiotemporal representation of IoMT networks since changes in mobility relationships over time can indicate volunteer organisation at the device and community *** Louvain community detection method is proposed to find communities of intelligent devices to reveal a value conscious participation of *** proposed bi-partite graph model is evaluated using a real-world scenario in transportation,confirming the main role of evolving communities in developing crowdsourcing IoMT networks.

关键词： Bi-partite graph modelling Internet of Moving Things crowdsourcing