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Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter

Light(Science & Applications) 2015年第1期4卷 430-437页

作者： Andreu Llobera Joan Juvert Alfredo Gonzalez-Fernandez Bergoi Ibarlucea Ester Carregal-Romero stephanus buttgenbach Cesar Fernandez-Sanchez Instituto de Microelectronica de Barcelona IMB-CNM(CSIC) Campus UAB08193 BellaterraSpain Institut fur Mikrotechnik Technische Universitat BraunschweigBraunschweig 38106Germany

A photonic lab on a chip(PhLOC),comprising a solid-state light emitter(SSLE)aligned with a biofunctionalized optofluidic multiple internal reflection(MIR)system,is presented.The SSLE is obtained by filling a microfluidic structure with a phenyltrimethoxysilane(PhTMOS)aqueous sol solution containing a fluorophore organic dye.After curing,the resulting xerogel solid structure retains the emitting properties of the fluorophore,which is evenly distributed in the xerogel matrix.Photostability studies demonstrate that after a total dose(at λ5365 nm)greater than 24 J cm^(-2),the xerogel emission decay is only 4.1%.To re-direct the emitted light,the SSLE includes two sets of air mirrors that surround the xerogel.Emission mapping of the SSLE demonstrates that alignment variations of 150 mm(between the SSLE and the external pumping light source)provide fluctuations in emitted light smaller than 5%.After this verification,the SSLE is monolithically implemented with a MIR,forming the PhLOC.Its performance is assessed by measuring quinolone yellow,obtaining a limit of detection(LOD)of(0.6060.01)mM.Finally,the MIR is selectively biofunctionalized with horseradish peroxidase(HRP)for the detection of hydrogen peroxide(H_(2)O_(2))target analyte,obtaining a LOD of(0.760.1)μM for H_(2)O_(2),confirming,for the first time,that solid-state xerogel-based emitters can be massively implemented in biofunctionalized PhLOCs.

关键词： medical devices photonic lab on a chip polymer technology solid-state light emitters sol–gel

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Application of UV depth lithography in micro system technology

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Optoelectronics Letters 2008年第1期4卷 1-4页

作者： stephanus Büttgenbach Marco Feldmann Institute for Microtechnology Technical University of Braunschweig Langer Kamp 8 38106 Braunschweig Germany

This letter reports on new types of photo resists allowing UV-light exposure of thick layers. Optimized processes Cthat for the positive resist AZ 9260 and the negative resist Epon SU-8 have been developed enabling the fabrication of high aspect ratio metallic and polymer micro components. The high potential of this technology will be demonstrated on the basis of two examples, a variable reluctance micro motor with compensated attractive force and an optical microphone developed for application in electro-magnetic interference environments.

关键词：微系统技术紫外线平版印刷术微电子学

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The in vitro effects of superoxide, some commercially available antioxidants and red palm oil on sperm motility

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Asian Journal of Andrology 2009年第6期11卷 695-702页

作者： Yapo Guillaume Aboua Stefan stephanus du Plessis Patricia Reichgelt Nicole Brooks Department of Biomedical Sciences Faculty of Health and Wellness Sciences Cape Peninsula University of TechnologyBellville 7535 South Africa Division of Medical Physiology Faculty of Health Sciences Stellenbosch University Tygerberg 7505 South Africa

In this study, two commercially available superoxide scavengers, tetrakis （1-methyl-4-pyridyl） porphyrin （Mn[III]TMPyP） and superoxide dismutase （SOD）, as well as red palm oil （RPO）, a natural vegetable oil, had been used to investigate their possible in vitro effects against the toxic effects of superoxide （O2＋） on human sperm motility. Semen samples were obtained from 12 normozoospermic healthy volunteer donors aged between 19 and 23 years. The O2＋donor 2,3-dimetoxyl-l,4-naphthoquinone （DMNQ）（2.5 μmol· L^-1-100 μmol· L^-1） was added to normozoospermic post-swim-up sperm in the presence or absence of Mn（III）TMPyP （50 μmol· L^-1）, SOD （50 IU） or RPO （0.1% or 0.5%）. Computer-assisted semen analysis was used to analyze various motility parameters. The parameters of interest were percentage of motile cells, progressive motility, rapid cells and static cells. Concentrations of higher than 25 μmol· L^-1 DMNQ were detrimental to sperm motility. Mn（III）TMPyP was able to attenuate the effect of O2＋ on the motility parameters. In vitro addition of SOD and RPO showed harmful effects on sperm motility.

关键词： antioxidants motility red palm oil superoxide

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Formidable females redux:male social integration into female networks and the value of dynamic multilayer networks

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Current Zoology 2021年第1期67卷 49-57页

作者： Tyler R.BONNELL ChloéVILETTE Christopher YOUNG stephanus Peter HENZI ouise BARRETT Department of Psychology University of Lethbridge4401 University DriveLethbridgeT1K 3M4Canada Applied Behavioural Ecology and Ecosystems Research Unit University of South AfricaFloridaGautengSouth Africa Endocrine Research Laboratory Mammal Research InstituteFaculty of Natural and Agricultural ScienceUniversity of PretoriaPretoriaSouth Africa

The development of multilayer network techniques is a boon for researchers who wish to understand how different interaction layers might influence each other,and how these in turn might influence group dynamics.Here,we investigate how integration between male and female grooming and aggression interaction networks influences male power trajectories in vervet monkeys Chlorocebus pygerythrus.Our previous analyses of this phenomenon used a monolayer approach,and our aim here is to extend these analyses using a dynamic multilayer approach.To do so,we constructed a temporal series of male and female interaction layers.We then used a multivariate multilevel autoregression model to compare cross-lagged associations between a male's centrality in the female grooming layer and changes in male Elo ratings.Our results confirmed our original findings:changes in male centrality within the female grooming network were weakly but positively tied to changes in their Elo ratings.However,the multilayer network approach offered additional insights into this social process,identifying how changes in a male's centrality cascade through the other network layers.This dynamic view indicates that the changes in Elo ratings are likely to be short-lived,but that male centrality within the female network had a much stronger impact throughout the multilayer network as a whole,especially on reducing intermale aggression(i.e.,aggression directed by males toward other males).We suggest that multilayer social network approaches can take advantage of increased amounts of social data that are more commonly collected these days,using a variety of methods.Such data are inherently multilevel and multilayered,and thus offer the ability to quantify more precisely the dynamics of animal social behaviors.

关键词： multilayer networks multilevel multivariate autoregressive model primate social dynamics social networks sociality time-aggregated networks vervet monkeys

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Poly(lactic acid)-starch/Expandable Graphite (PLA-starch/EG) Flame Retardant Composites

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Journal of Renewable Materials 2018年第1期6卷 26-37页

作者： Mfiso Emmanuel Mngomezulu Adriaan stephanus Luyt Steve Anthony Chapple Maya Jacob John Department of Chemistry University of the Free State(Qwaqwa Campus)Private Bag X13Phuthaditjhaba 9866South Africa Center for Advanced Materials Qatar UniversityPO Box 2713DohaQatar CSIR Materials Science and Manufacturing Polymers and Composites Competence AreaPO Box 1124Port Elizabeth 6000South Africa Department of Chemistry Faculty of ScienceNelson Mandela Metropolitan UniversityPO Box 1600Port Elizabeth 6000South Africa

This work reports on the effect of commercial expandable graphite(EG)on the flammability and thermal decomposition properties of PLA-starch blend.The PLA-starch/EG composites were prepared by melt-mixing and their thermal stability,volatile pyrolysis products and flammability characteristics were investigated.The char residues of the composites,after combustion in a cone calorimeter,were analyzed with environmental scanning electron microscopy(ESEM).The thermal decomposition stability of the composites improved in the presence of EG.However,the char content was less than expected as per the combination of the wt%EG added into PLA-starch and the%residue of PLA-starch.The flammability performance of the PLA-starch/EG composites improved,especially at 15 wt%EG content,due to a thick and strong worm-like char structure.The peak heat release rate(PHRR)improved by 74%,the total smoke production(TSP)by 40%and the specific extinction area(SEA)by 55%.The improvements are attributed to the ability of EG to exfoliate at increased temperatures during which time three effects occurred:(i)cooling due to an endothermic exfoliation process,(ii)dilution due to release of H2O,SO2 and CO2 gases,and (iii)formation of a protective intumescent char layer.However,the CO and CO2 yields were found to be unfavorably high due to the presence of EG.

关键词： Poly(lactic acid)-starch composites expandable graphite thermal stability flammability properties

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Multi-agent Based Protection on Highly Dominated Distributed Energy Resources

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Energy and Power Engineering 2013年第4期5卷 927-931页

作者： stephanus A. Ananda Jyh-Cherng Gu Department of Electrical Engineering National Taiwan University of Science and Technology Department of Electrical Engineering National Taiwan University of Science and Technology.

Highly penetration of Distributed Energy Resources (DER) on the grid systems nowadays makes the systems grow dynamically. The system become more complex and the protection system become more complicated. The protection relay should accommodate the system changes according to the system conditions and topologies. As part of developmental aspect of Distributed Artificial Intelligent, Multi Agent System (MAS) is a challenging method for improving the intelligent properties of relay protection. This paper introduces the use of MAS approach on radial distribution system protection dominated with DER using dispersed adaptive rule-based protection supported by distributed database agent. The simulation results confirmed that the proposed algorithm can respond within 15.05 ms.

关键词： Multi-Agent System Power System Protection Distributed Energy Resources

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Integration of a Dolomite Risk Management Programme in Local Governance in South Africa: Tlokwe City Council

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Journal of Environmental Protection 2016年第7期7卷 1041-1049页

作者： Abraham S. Potgieter stephanus J. Pretorius Izak Jacobus Van Der Walt AGES North West Potchefstroom South Africa School of Environmental Sciences North-West University Potchefstroom South Africa

Development on dolomite poses a risk to people and their assets living on it. Although development in the Tlokwe City Council (TCC) broadly took cognisance of the presence of dolomite, some parts of the township had been developed on dolomite. Therefore, since the Council is accountable for safe development within its jurisdiction, a Dolomite Risk Management Programme (DRMPr) should be put in place according to prevailing legislation, standards and guidelines. For long-term mitigation measures to be implemented successfully, the DRMPr should be integrated and aligned within the existing structure and processes of the Council. This exercise is described, with some challenges identified and recommendations made, for consideration in the same or similar programmes in future.

关键词： Dolomite Hazard Development Risk Management Mitigation Strategy Plan Programme Integrate Implement

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《Reflection》

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海峡影艺 2013年第3期 68-页

作者： stephanus

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计算机辅助微技术设计

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工程设计学报 2003年第2期10卷 62-65页

作者： [德]Christoph Germer Ulli Hansen Hans-Jochamin Franke stephanus Büttgenbach 桂贵生(译) 刘全坤(译校) 不详 Braunschweig工业大学设计学机械零件和精密零件研究所合肥工业大学机械与汽车工程学院合肥工业大学研究生部 230009

微机构和微系统设计需要综合应用许多不同的制造工艺,因而是非常复杂的.介绍了用于计算机辅助微技术设计的系统,它综合工艺描述工具,并将其输出存储在全程数据模型中.

关键词：计算机辅助微技术设计微机构硅基微系统数据储存

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Association between TNF-a and IL-1β genotypes vs Helicobacter pylori infection in Indonesia

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World Journal of Gastroenterology 2013年第46期19卷 8758-8763页

作者： Yang Zhao Jing-Wen Wang Tsutomu Tanaka Akihiro Hosono Ryosuke Ando Shinkan Tokudome Soeripto FX Ediati Triningsih Tegu Triono Suwignyo Sumoharjo EY Wenny Astuti Achwan stephanus Gunawan Yu-Min Li Department of Cancer Center Second Hospital of Lanzhou University Department of Public Health Nagoya City University Graduate School of Medical Sciences Bell Research Center for Reproductive Health and Cancer Medical Corporation Kishokai Department of Nephro-urology Nagoya City University Graduate School of Medical Sciences Department of Nutritional Epidemiology National Institute of Health and Nutrition Department of Anatomic Pathology Gadjah Mada University Faculty of MedicineDaerah Istimewa Yogyakarta Department of Biomedical Research Unit General Hospital of West Nusa Tenggara Province First Department of General Surgery Second Hospital of Lanzhou University Gansu Provincial Key Laboratory of Digestive System Tumors

AIM:To investigate the correlation between the Helicobacter pylori(H.pylori)infection and host genetic background of healthy populations in Indonesia.METHODS:In March 2007,epidemiological studies were undertaken on the general population of a city in Indonesia(Mataram,Lombok).The participants included 107 men and 187 women,whose ages ranged from6 to 74 years old,with an average age of 34.0(±14.4)(±SD).The H.pylori of subject by UBT method determination,and through the polymerase chain reaction with confronting two-pair primers(PCR-CTPP)method parsing the single nucleotide polymorphism of interleukin(IL)-8,IL-4,IL-1β,CD14,tumor necrosis factor(TNF-a)and tyrosine-protein phosphates non-receptor type 11(PTPN11)genotypes.The experimental data were analyzed by the statistical software SAS.RESULTS:The H.pylori infection rates in the healthy Indonesian population studied were 8.4%for men and12.8%for women;no obvious differences were noted for H.pylori infection rates by sex or age.TC genotypes of IL-4,TC and CC genotypes of TNF-a,and GA genotypes of PTPN11,were higher in frequency.Both CC and TC genotype of TNF-a T-1031C loci featured higher expressions in the healthy Indonesian population Indonesia studied of(OR=1.99;95%CI:0.67-5.89)and(OR=1.66;95%CI:0.73-3.76),respectively.C allele of IL-1βT-31C gene locus was at a higher risk(OR=1.11;95%CI:0.70-1.73)of H.pylori infection,but no statistical significance was found in our study.CONCLUSION:We reveal that the association between the TNF-a and IL-1βgenotypes may be the susceptibility of H.pylori in the studied population.

关键词： Helicobacter pylori Tumor necrosis factor Interleukin-1β Infection Allele

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